WO1996017082A3 - Compound microsatellite primers for the detection of genetic polymorphisms - Google Patents

Compound microsatellite primers for the detection of genetic polymorphisms Download PDF

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Publication number
WO1996017082A3
WO1996017082A3 PCT/US1995/015150 US9515150W WO9617082A3 WO 1996017082 A3 WO1996017082 A3 WO 1996017082A3 US 9515150 W US9515150 W US 9515150W WO 9617082 A3 WO9617082 A3 WO 9617082A3
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WO
WIPO (PCT)
Prior art keywords
compound
primers
detection
genetic polymorphisms
phase
Prior art date
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Ceased
Application number
PCT/US1995/015150
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French (fr)
Other versions
WO1996017082A2 (en
Inventor
Michele Morgante
Julie Marie Vogel
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EIDP Inc
Original Assignee
EI Du Pont de Nemours and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co
Priority to DE69507646T priority Critical patent/DE69507646T2/en
Priority to NZ298236A priority patent/NZ298236A/en
Priority to EP95942451A priority patent/EP0804618B1/en
Priority to JP8517186A priority patent/JPH10509594A/en
Priority to CA 2203143 priority patent/CA2203143A1/en
Priority to AU43674/96A priority patent/AU704660B2/en
Publication of WO1996017082A2 publication Critical patent/WO1996017082A2/en
Publication of WO1996017082A3 publication Critical patent/WO1996017082A3/en
Anticipated expiration legal-status Critical
Priority to US08/849,021 priority patent/US5955276A/en
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/172—Haplotypes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Compound microsatellites used as self-anchoring primers for screening genomes to detect polymorphisms are provided. The preferred compound microsatellite primers are perfect compound dinucleotide primers wherein the adjacent dinucleotide pairs contain a common nucleotide which is in-phase across the compound junction. In a useful embodiment, these in-phase compound primers have been adapted to other known screening assays including variations of the AFLP assays.
PCT/US1995/015150 1994-11-28 1995-11-21 Compound microsatellite primers for the detection of genetic polymorphisms Ceased WO1996017082A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
DE69507646T DE69507646T2 (en) 1994-11-28 1995-11-21 MICROSATELLITE CONNECTION FOR DETECTING GENETIC POLYMORPHISMS
NZ298236A NZ298236A (en) 1994-11-28 1995-11-21 Primers for detecting genetic polymorphisms
EP95942451A EP0804618B1 (en) 1994-11-28 1995-11-21 Compound microsatellite primers for the detection of genetic polymorphisms
JP8517186A JPH10509594A (en) 1994-11-28 1995-11-21 Composite microsatellite primers for detection of genetic polymorphism
CA 2203143 CA2203143A1 (en) 1994-11-28 1995-11-21 Compound microsatellite primers for the detection of genetic polymorphisms
AU43674/96A AU704660B2 (en) 1994-11-28 1995-11-21 Compound microsatellite primers for the detection of genetic polymorphisms
US08/849,021 US5955276A (en) 1994-11-28 1997-07-03 Compound microsatellite primers for the detection of genetic polymorphisms

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US34645694A 1994-11-28 1994-11-28
US08/346,456 1994-11-28

Publications (2)

Publication Number Publication Date
WO1996017082A2 WO1996017082A2 (en) 1996-06-06
WO1996017082A3 true WO1996017082A3 (en) 1996-08-08

Family

ID=23359478

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1995/015150 Ceased WO1996017082A2 (en) 1994-11-28 1995-11-21 Compound microsatellite primers for the detection of genetic polymorphisms

Country Status (10)

Country Link
US (1) US5955276A (en)
EP (1) EP0804618B1 (en)
JP (1) JPH10509594A (en)
AR (1) AR000215A1 (en)
AU (1) AU704660B2 (en)
DE (1) DE69507646T2 (en)
IL (1) IL116135A0 (en)
NZ (1) NZ298236A (en)
WO (1) WO1996017082A2 (en)
ZA (1) ZA9510097B (en)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0721987A1 (en) * 1995-01-16 1996-07-17 Keygene N.V. Amplification of simple sequence repeats
DE19525284A1 (en) * 1995-06-28 1997-01-02 Inst Pflanzengenetik & Kultur Microsatellite markers for plants of the species Triticum aestivum and of the Tribus Triticeae and their use
US6218119B1 (en) 1996-01-16 2001-04-17 Keygene, N. V. Amplification of simple sequence repeats
WO1998042867A1 (en) * 1997-03-21 1998-10-01 Greg Firth Extraction and utilisation of vntr alleles
IL131610A0 (en) * 1997-03-21 2001-01-28 Greg Firth Extraction and utilisation of vntr alleles
JP2002503954A (en) 1997-04-01 2002-02-05 グラクソ、グループ、リミテッド Nucleic acid amplification method
US6054276A (en) 1998-02-23 2000-04-25 Macevicz; Stephen C. DNA restriction site mapping
US6500616B1 (en) 1998-04-16 2002-12-31 Case Western Reserve University Methods of monitoring genomic integrity and detecting genomic destabilization of plant cells in tissue culture
US6287825B1 (en) * 1998-09-18 2001-09-11 Molecular Staging Inc. Methods for reducing the complexity of DNA sequences
AR021815A1 (en) 1998-09-18 2002-08-07 Univ Nebraska Lincoln HIGH RESOLUTION ANALYSIS OF GENOMAS.
US6703228B1 (en) 1998-09-25 2004-03-09 Massachusetts Institute Of Technology Methods and products related to genotyping and DNA analysis
EP1001037A3 (en) * 1998-09-28 2003-10-01 Whitehead Institute For Biomedical Research Pre-selection and isolation of single nucleotide polymorphisms
AU6372099A (en) * 1998-10-16 2000-05-08 Keygene N.V. Method for the generation of dna fingerprints
AU1402900A (en) * 1998-11-09 2000-05-29 Methexis N.V. Restricted amplicon analysis
CA2352476A1 (en) * 1998-12-04 2000-06-15 Keygene N.V. Array and method for analysing nucleic acid sequences
US7166432B2 (en) 1999-03-12 2007-01-23 Integragen Compositions and methods for genetic analysis
DE19911130A1 (en) * 1999-03-12 2000-09-21 Hager Joerg Methods for identifying chromosomal regions and genes
EP1173612B1 (en) * 1999-04-09 2004-09-29 Keygene N.V. METHOD FOR THE DETECTION AND/OR ANALYSIS, BY MEANS OF PRIMER EXTENSION TECHNIQUES, OF SINGLE NUCLEOTIDE POLYMORPHISMS IN RESTRICTION FRAGMENTS, IN PARTICULAR IN AMPLIFIED RESTRICTION FRAGMENTS GENERATED USING AFLP$m(3)
US6420117B1 (en) 1999-09-14 2002-07-16 The University Of Georgia Research Foundation, Inc. Miniature inverted repeat transposable elements and methods of use
EP1130114A1 (en) * 2000-03-03 2001-09-05 Dr. van Haeringen Laboratorium B.V. Universal variable fragments
WO2001083813A1 (en) * 2000-05-02 2001-11-08 Centre National De La Recherche Scientifique Identification of genetic markers
EP1282729A2 (en) * 2000-05-15 2003-02-12 Keygene N.V. Microsatellite-aflp
EP1158056A1 (en) * 2000-05-15 2001-11-28 Keygene N.V. Microsatellite-AFLP
EP1170379A1 (en) * 2000-06-30 2002-01-09 Centre National de Genotypage Sample generation for genotyping by mass spectrometry
US7695901B2 (en) * 2000-07-26 2010-04-13 North Carolina State University Identification of poinsettia cultivars
FR2822711B1 (en) * 2001-03-28 2003-06-13 Oreal TREATMENT DEVICE COMPRISING AN ENVELOPE DEFINING A CAVITY IN WHICH A PART OF THE BODY MAY BE ENGAGED
US7176303B2 (en) * 2003-11-06 2007-02-13 Isis Pharmaceuticals, Inc. Modulation of STAT5 expression
US20040110142A1 (en) * 2002-12-09 2004-06-10 Isis Pharmaceuticals Inc. Modulation of AAC-11 expression
US7115370B2 (en) * 2002-06-05 2006-10-03 Capital Genomix, Inc. Combinatorial oligonucleotide PCR
JPWO2004044243A1 (en) * 2002-11-13 2006-03-09 株式会社日立ハイテクノロジーズ Nucleic acid amplification method and device
WO2005014796A2 (en) * 2003-08-08 2005-02-17 Invitrogen Corporation Methods and compositions for seamless cloning of nucleic acid molecules
KR100645445B1 (en) 2004-09-09 2006-11-15 강원대학교산학협력단 Method for producing markers for detecting polymorphism of plants
DK2302070T3 (en) 2005-06-23 2012-11-26 Keygene Nv Strategies for the identification and detection of high-throughput polymorphisms
WO2007037678A2 (en) 2005-09-29 2007-04-05 Keygene N.V. High throughput screening of mutagenized populations
US10316364B2 (en) 2005-09-29 2019-06-11 Keygene N.V. Method for identifying the source of an amplicon
CN103937899B (en) 2005-12-22 2017-09-08 凯津公司 Method for the high flux polymorphic detection based on AFLP
ES2301342B1 (en) * 2006-03-14 2009-05-01 Oryzon Genomics, S.A. "METHOD OF ANALYSIS OF NUCLEIC ACIDS".
EP3239304B1 (en) 2006-04-04 2020-08-19 Keygene N.V. High throughput detection of molecular markers based on aflp and high troughput sequencing
DE102007010311A1 (en) * 2007-02-23 2008-08-28 Thines, Marco, Dr. Organism-specific hybridizable nucleic acid molecule
US20140053294A1 (en) * 2010-11-19 2014-02-20 The University Of Queensland Pongamia Genetic Markers and Method of Use
CN110172525A (en) * 2019-06-26 2019-08-27 广西壮族自治区林业科学研究院 Forest difference expression gene SSR primer sets and polymorphism SSR marker development approach
EP4055162A1 (en) * 2019-11-05 2022-09-14 Florida State University Research Foundation, Inc. Evaluating genomic variation using repetitive nucleic acid sequences
US12601008B2 (en) 2021-11-04 2026-04-14 Florida State University Research Foundation, Inc. Using hairpin formation to identify DNA and RNA sequences having a target nucleic acid sequence

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991018114A1 (en) * 1990-05-22 1991-11-28 Medical Research Council Polynucleotide amplification
WO1992013969A1 (en) * 1991-01-31 1992-08-20 Baylor College Of Medicine Dna typing with short tandem repeat polymorphisms and identification of polymorphic short tandem repeats
EP0534858A1 (en) * 1991-09-24 1993-03-31 Keygene N.V. Selective restriction fragment amplification : a general method for DNA fingerprinting
WO1993007166A1 (en) * 1991-10-09 1993-04-15 The Salk Institute For Biological Studies Polymorphic locus
EP0552545A1 (en) * 1992-01-17 1993-07-28 Pioneer Hi-Bred International, Inc. Detection of polymorphisms in simple sequence repeats using oligonucleotide ligation
WO1994017203A1 (en) * 1993-01-29 1994-08-04 The Governors Of The University Of Alberta Amplified dna fingerprinting method for detecting genomic variation
WO1995015400A1 (en) * 1993-12-03 1995-06-08 The Johns Hopkins University Genotyping by simultaneous analysis of multiple microsatellite loci

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8818020D0 (en) * 1988-07-28 1988-09-01 Ici Plc Method for amplification of nucleotide sequences
US5206137A (en) * 1988-09-08 1993-04-27 Lifecodes Corporation Compositions and methods useful for genetic analysis
DE3834636A1 (en) * 1988-10-11 1990-04-19 Max Planck Gesellschaft METHOD FOR ANALYZING LENGTH POLYMORPHISMS IN DNA AREAS
US5126239A (en) * 1990-03-14 1992-06-30 E. I. Du Pont De Nemours And Company Process for detecting polymorphisms on the basis of nucleotide differences
WO1992013968A1 (en) * 1991-02-07 1992-08-20 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Monolocus-specific hypervariable probes

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991018114A1 (en) * 1990-05-22 1991-11-28 Medical Research Council Polynucleotide amplification
WO1992013969A1 (en) * 1991-01-31 1992-08-20 Baylor College Of Medicine Dna typing with short tandem repeat polymorphisms and identification of polymorphic short tandem repeats
EP0534858A1 (en) * 1991-09-24 1993-03-31 Keygene N.V. Selective restriction fragment amplification : a general method for DNA fingerprinting
WO1993007166A1 (en) * 1991-10-09 1993-04-15 The Salk Institute For Biological Studies Polymorphic locus
EP0552545A1 (en) * 1992-01-17 1993-07-28 Pioneer Hi-Bred International, Inc. Detection of polymorphisms in simple sequence repeats using oligonucleotide ligation
WO1994017203A1 (en) * 1993-01-29 1994-08-04 The Governors Of The University Of Alberta Amplified dna fingerprinting method for detecting genomic variation
WO1995015400A1 (en) * 1993-12-03 1995-06-08 The Johns Hopkins University Genotyping by simultaneous analysis of multiple microsatellite loci

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
D.L. BROWNE AND M. LITT: "Characterization of (CA)n microsatellites with degenerate sequencing primers", NUCLEIC ACIDS RESEARCH, vol. 20, no. 1, 11 January 1992 (1992-01-11), IRL PRESS LIMITED,OXFORD,ENGLAND, pages 141, XP002002457 *
E. ZIETKIEWICZ ET AL.: "Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction", GENOMICS, vol. 20, no. 2, 15 March 1994 (1994-03-15), ACADEMIC PRESS INC., NY, US, pages 176 - 183, XP000569780 *
GRIST S A ET AL: "DINUCLEOTIDE REPEAT POLYMORPHISMS ISOLATED BY THE POLYMERASE CHAIN REACTION", BIOTECHNIQUES, vol. 15, no. 2, 1 August 1993 (1993-08-01), pages 304 - 309, XP000382830 *
J.L.WEBER AND P.E. MAY: "Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction", AM.J. HUMAN. GENET., vol. 44, 1989, AM.SOC.HUM.GENET.,CHICAGO,US, pages 388 - 396, XP000431459 *
K.-S. WU ET AL.: "Detection of microsatellite polymorphisms without cloning", NUCLEIC ACIDS RESEARCH, vol. 22, no. 15, 11 August 1994 (1994-08-11), IRL PRESS LIMITED,OXFORD,ENGLAND, pages 3257 - 3258, XP002002453 *
M. LYNN ET AL.: "mapping maize microsatellites and polymerase chain reaction confirmation of the target repeats using a CT primer", GENOME, vol. 36, no. 5, October 1993 (1993-10-01), OTTAWA, CA, pages 884 - 889, XP000569548 *
M. MORGANTE AND A.M. OLIVERI: "PCR-amplified microsatellites as markers in plant genetics", PLANT JOURNAL, vol. 3, no. 1, January 1993 (1993-01-01), BLACKWELL SCIENCE LTD, OXFORD, UK, pages 175 - 182, XP002002455 *
M.A. S. MAROOF ET AL.: "Extraordinarily polymorphic microsatellite DNA in barley: Species diversity, chromosomal locations, and population dynamics", PROC. NATL.ACAD SCI., vol. 91, no. 12, June 1994 (1994-06-01), NATL. ACAD SCI.,WASHINGTON,DC,US;, pages 5466 - 5470, XP002002454 *
M.A.R.YUILLE ET AL.: "Rapid determination of sequences flanking microsatellites", NUCLEIC ACIDS RESEARCH, vol. 19, no. 8, 25 April 1991 (1991-04-25), IRL PRESS LIMITED,OXFORD,ENGLAND, pages 1950, XP002002456 *
Z. WANG ET AL.: "Survey of plant short tandem repeats", THEORETICAL AND APPLIED GENETICS, no. 88, 1901 - April 1994 (1994-04-01), SPRINGER INTERNATIONAL, NY, US, pages 1 - 6, XP000569790 *

Also Published As

Publication number Publication date
AR000215A1 (en) 1997-05-21
JPH10509594A (en) 1998-09-22
IL116135A0 (en) 1996-01-31
DE69507646D1 (en) 1999-03-11
ZA9510097B (en) 1997-05-28
EP0804618B1 (en) 1999-01-27
US5955276A (en) 1999-09-21
WO1996017082A2 (en) 1996-06-06
EP0804618A2 (en) 1997-11-05
AU4367496A (en) 1996-06-19
AU704660B2 (en) 1999-04-29
DE69507646T2 (en) 1999-09-16
NZ298236A (en) 1999-01-28

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