WO1996017956A3 - Method of detection of nucleic acids with a specific sequence composition - Google Patents

Method of detection of nucleic acids with a specific sequence composition Download PDF

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Publication number
WO1996017956A3
WO1996017956A3 PCT/US1995/015944 US9515944W WO9617956A3 WO 1996017956 A3 WO1996017956 A3 WO 1996017956A3 US 9515944 W US9515944 W US 9515944W WO 9617956 A3 WO9617956 A3 WO 9617956A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
detection
nucleic acids
specific
novel
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1995/015944
Other languages
French (fr)
Other versions
WO1996017956A2 (en
Inventor
Susan Weininger
Arthur M Weininger
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gene Pool Inc
Original Assignee
Gene Pool Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gene Pool Inc filed Critical Gene Pool Inc
Priority to DK95943035T priority Critical patent/DK0796344T3/en
Priority to AU44189/96A priority patent/AU4418996A/en
Priority to EP95943035A priority patent/EP0796344B1/en
Priority to DE69535384T priority patent/DE69535384T2/en
Priority to BR9509977A priority patent/BR9509977A/en
Priority to JP8517797A priority patent/JPH10510157A/en
Publication of WO1996017956A2 publication Critical patent/WO1996017956A2/en
Priority to NO19972611A priority patent/NO321557B1/en
Anticipated expiration legal-status Critical
Publication of WO1996017956A3 publication Critical patent/WO1996017956A3/en
Priority to NO20052968A priority patent/NO20052968D0/en
Priority to US12/493,877 priority patent/US8114962B2/en
Priority to US12/899,251 priority patent/US20110172169A1/en
Priority to US13/093,127 priority patent/US20110263045A1/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

This invention is a novel method for detecting and localizing specific nucleic acid sequences in a sample with a high degree of sensitivity and specificity. The method and novel compositions used in the method involve the use of Probe Nucleic Acids, the production of nucleic acid binding regions and the use of nucleic acid Target Binding Assemblies to detect and localize specific Target Nucleic Acids. The detection and localization of the Target Nucleic Acid is accomplished even in the presence of nucleic acids which have similar sequences. The method provides for a high degree of amplification of the signal produced by each specific binding event. In particular, methods and compositions are presented for the detection of HIV and HPV nucleic acid in samples. These methods and compositions find use in diagnosis of disease, genetic monitoring, forensics, and analysis of nucleic acid mixtures. Some of the novel compositions used in the detection method are useful in preventing or treating pathogenic conditions.
PCT/US1995/015944 1994-12-09 1995-12-07 Method of detection of nucleic acids with a specific sequence composition Ceased WO1996017956A2 (en)

Priority Applications (11)

Application Number Priority Date Filing Date Title
DK95943035T DK0796344T3 (en) 1994-12-09 1995-12-07 Method for Detecting Nucleic Acids with a Specific Sequence Composition
AU44189/96A AU4418996A (en) 1994-12-09 1995-12-07 Method of detection of nucleic acids with a specific sequence composition
EP95943035A EP0796344B1 (en) 1994-12-09 1995-12-07 Method of detection of nucleic acids with a specific sequence composition
DE69535384T DE69535384T2 (en) 1994-12-09 1995-12-07 METHOD FOR DETECTION OF NUCLEIC ACID WITH A SPECIFIC SEQUENCE COMPOSITION
BR9509977A BR9509977A (en) 1994-12-09 1995-12-07 Detection process of nucleic acids with a specific sequence composition
JP8517797A JPH10510157A (en) 1994-12-09 1995-12-07 Method for detecting nucleic acid having specific sequence composition
NO19972611A NO321557B1 (en) 1994-12-09 1997-06-06 Procedure for Detecting Nucleic Acids with a Specific Sequence Composition
NO20052968A NO20052968D0 (en) 1994-12-09 2005-06-17 Procedure for Detecting Nucleic Acids with a Specific Sequence Composition.
US12/493,877 US8114962B2 (en) 1994-12-09 2009-06-29 Method of detection of nucleic acids with a specific sequence composition
US12/899,251 US20110172169A1 (en) 1994-12-09 2010-10-06 Method of Detection of Nucleic Acids with a Specific Sequence Composition
US13/093,127 US20110263045A1 (en) 1994-12-09 2011-04-25 Method of Detection of Nucleic Acids With a Specific Sequence Composition

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/353,476 1994-12-09
US08/353,476 US5871902A (en) 1994-12-09 1994-12-09 Sequence-specific detection of nucleic acid hybrids using a DNA-binding molecule or assembly capable of discriminating perfect hybrids from non-perfect hybrids

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
US08/353,476 Continuation-In-Part US5871902A (en) 1994-12-09 1994-12-09 Sequence-specific detection of nucleic acid hybrids using a DNA-binding molecule or assembly capable of discriminating perfect hybrids from non-perfect hybrids

Related Child Applications (3)

Application Number Title Priority Date Filing Date
US08/860,844 A-371-Of-International US20030104361A1 (en) 1994-12-09 1995-12-07 Method of detection of nucleic acids with a specific sequence composition
US08860844 A-371-Of-International 1995-12-07
US10/407,543 Continuation US20030175789A1 (en) 1994-12-09 2003-04-03 Method of detection of nucleic acids with a specific sequence composition

Publications (2)

Publication Number Publication Date
WO1996017956A2 WO1996017956A2 (en) 1996-06-13
WO1996017956A3 true WO1996017956A3 (en) 1997-07-24

Family

ID=23389290

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1995/015944 Ceased WO1996017956A2 (en) 1994-12-09 1995-12-07 Method of detection of nucleic acids with a specific sequence composition

Country Status (15)

Country Link
US (1) US5871902A (en)
EP (2) EP0796344B1 (en)
JP (2) JPH10510157A (en)
CN (2) CN1255551C (en)
AT (1) ATE353372T1 (en)
BR (1) BR9509977A (en)
CA (1) CA2206127A1 (en)
DE (1) DE69535384T2 (en)
DK (1) DK0796344T3 (en)
ES (1) ES2279516T3 (en)
IL (2) IL150713A (en)
MX (1) MX9704281A (en)
NO (2) NO321557B1 (en)
PT (1) PT796344E (en)
WO (1) WO1996017956A2 (en)

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CA2196419C (en) 1994-08-20 2007-08-21 Yen Choo Improvements in or relating to binding proteins for recognition of dna
US20030104361A1 (en) * 1997-09-29 2003-06-05 Susan Weininger Method of detection of nucleic acids with a specific sequence composition
US5871902A (en) * 1994-12-09 1999-02-16 The Gene Pool, Inc. Sequence-specific detection of nucleic acid hybrids using a DNA-binding molecule or assembly capable of discriminating perfect hybrids from non-perfect hybrids
US5853990A (en) 1996-07-26 1998-12-29 Edward E. Winger Real time homogeneous nucleotide assay
JP2001501825A (en) 1996-10-04 2001-02-13 ダコ アクティーゼルスカブ Novel probe for detection of Mycobacteria
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US6410248B1 (en) 1998-01-30 2002-06-25 Massachusetts Institute Of Technology General strategy for selecting high-affinity zinc finger proteins for diverse DNA target sites
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EP1820864A3 (en) 2008-01-23
NO321557B1 (en) 2006-05-29
NO20052968L (en) 1997-08-11
CN1255551C (en) 2006-05-10
IL150713A (en) 2011-02-28
WO1996017956A2 (en) 1996-06-13
DE69535384T2 (en) 2007-09-13
ATE353372T1 (en) 2007-02-15
MX9704281A (en) 1998-07-31
CN1176663A (en) 1998-03-18
BR9509977A (en) 1998-06-09
CN1936020A (en) 2007-03-28
EP0796344A1 (en) 1997-09-24
NO20052968D0 (en) 2005-06-17
US5871902A (en) 1999-02-16
NO972611D0 (en) 1997-06-06
CA2206127A1 (en) 1996-06-13
PT796344E (en) 2007-05-31
IL150713A0 (en) 2003-02-12
ES2279516T3 (en) 2007-08-16
DK0796344T3 (en) 2007-06-04
EP0796344B1 (en) 2007-02-07
NO972611L (en) 1997-08-11
JP2006320326A (en) 2006-11-30
DE69535384D1 (en) 2007-03-22
EP1820864A2 (en) 2007-08-22
IL116321A (en) 2004-09-27
IL116321A0 (en) 1996-03-31
JPH10510157A (en) 1998-10-06
JP4598722B2 (en) 2010-12-15

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