WO1996040939A3 - Expression of functional vertebrate phospholipases in yeast - Google Patents

Expression of functional vertebrate phospholipases in yeast Download PDF

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Publication number
WO1996040939A3
WO1996040939A3 PCT/US1996/010002 US9610002W WO9640939A3 WO 1996040939 A3 WO1996040939 A3 WO 1996040939A3 US 9610002 W US9610002 W US 9610002W WO 9640939 A3 WO9640939 A3 WO 9640939A3
Authority
WO
WIPO (PCT)
Prior art keywords
yeast
phospholipases
expression
vertebrate
functional
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1996/010002
Other languages
French (fr)
Other versions
WO1996040939A2 (en
Inventor
Jeremy I Paul
James R Broach
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cadus Pharmaceutical Corp
Original Assignee
Cadus Pharmaceutical Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cadus Pharmaceutical Corp filed Critical Cadus Pharmaceutical Corp
Priority to JP50214497A priority Critical patent/JP2001507201A/en
Priority to EP96918448A priority patent/EP0832248A2/en
Priority to AU61108/96A priority patent/AU6110896A/en
Publication of WO1996040939A2 publication Critical patent/WO1996040939A2/en
Publication of WO1996040939A3 publication Critical patent/WO1996040939A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4722—G-proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
    • C07K14/723—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH receptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Toxicology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Endocrinology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Yeast cells comprising a heterologous phospholipase C protein and methods for their use in drug screening assays to identify a modulator (i.e. agonist or antagonist) of a phospholipase C are described.
PCT/US1996/010002 1995-06-07 1996-06-07 Expression of functional vertebrate phospholipases in yeast Ceased WO1996040939A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP50214497A JP2001507201A (en) 1995-06-07 1996-06-07 Expression of functional vertebrate phospholipase in yeast
EP96918448A EP0832248A2 (en) 1995-06-07 1996-06-07 Expression of functional vertebrate phospholipases in yeast
AU61108/96A AU6110896A (en) 1995-06-07 1996-06-07 Expression of functional vertebrate phospholipases in yeast

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US48163295A 1995-06-07 1995-06-07
US08/481,632 1995-06-07

Publications (2)

Publication Number Publication Date
WO1996040939A2 WO1996040939A2 (en) 1996-12-19
WO1996040939A3 true WO1996040939A3 (en) 1997-01-23

Family

ID=23912754

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1996/010002 Ceased WO1996040939A2 (en) 1995-06-07 1996-06-07 Expression of functional vertebrate phospholipases in yeast

Country Status (5)

Country Link
EP (1) EP0832248A2 (en)
JP (1) JP2001507201A (en)
AU (1) AU6110896A (en)
CA (1) CA2220459A1 (en)
WO (1) WO1996040939A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU7820498A (en) * 1997-06-06 1998-12-21 University Of Iowa Research Foundation, The (candida albicans) two-component hybrid kinase gene, canik1, and use thereof
AU2001250774B2 (en) * 2000-02-08 2005-01-27 Sangamo Biosciences, Inc. Cells expressing zinc finger proteins for drug discovery
WO2020076697A1 (en) * 2018-10-08 2020-04-16 Novozymes A/S Enzyme-expressing yeast for ethanol production
CN114891765B (en) * 2019-11-21 2023-12-19 天津科技大学 A kind of phospholipase and its application
CN111004787B (en) * 2020-01-06 2022-05-10 江南大学 A kind of Streptomyces phospholipase D mutant, transformation method and application thereof
CN114525266B (en) * 2022-02-22 2023-06-20 华南理工大学 A kind of phospholipase D mutant derived from Antarctic bacteria and its application

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992005244A1 (en) * 1990-09-13 1992-04-02 Duke University Expression of g protein coupled receptors in yeast
DE4138621A1 (en) * 1991-11-25 1993-06-17 Boehringer Ingelheim Int METHOD FOR SCREENING SUBSTANCES WITH MODULATING EFFECT ON A RECEPTACLE-RELATED CELLULAR SIGNAL TRANSMISSION PATH
WO1994023025A1 (en) * 1993-03-31 1994-10-13 Cadus Pharmaceuticals, Inc. Yeast cells engineered to produce pheromone system protein surrogates, and uses therefor
WO1995008624A1 (en) * 1993-09-24 1995-03-30 Shionogi & Co., Ltd. HUMAN PHOSPHOLIPASE C-α AND DNA SEQUENCE CODING FOR THE SAME
WO1995021925A1 (en) * 1994-02-14 1995-08-17 American Cyanamid Company Heterologous g protein coupled receptors expressed in yeast, their fusion with g proteins and use thereof in bioassay
WO1995030012A1 (en) * 1994-04-26 1995-11-09 Cadus Pharmaceutical Corporation Functional expression of mammalian adenylyl cyclase in yeast

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992005244A1 (en) * 1990-09-13 1992-04-02 Duke University Expression of g protein coupled receptors in yeast
DE4138621A1 (en) * 1991-11-25 1993-06-17 Boehringer Ingelheim Int METHOD FOR SCREENING SUBSTANCES WITH MODULATING EFFECT ON A RECEPTACLE-RELATED CELLULAR SIGNAL TRANSMISSION PATH
WO1994023025A1 (en) * 1993-03-31 1994-10-13 Cadus Pharmaceuticals, Inc. Yeast cells engineered to produce pheromone system protein surrogates, and uses therefor
WO1995008624A1 (en) * 1993-09-24 1995-03-30 Shionogi & Co., Ltd. HUMAN PHOSPHOLIPASE C-α AND DNA SEQUENCE CODING FOR THE SAME
EP0731164A1 (en) * 1993-09-24 1996-09-11 Shionogi & Co., Ltd. HUMAN PHOSPHOLIPASE C-$g(a) AND DNA SEQUENCE CODING FOR THE SAME
WO1995021925A1 (en) * 1994-02-14 1995-08-17 American Cyanamid Company Heterologous g protein coupled receptors expressed in yeast, their fusion with g proteins and use thereof in bioassay
WO1995030012A1 (en) * 1994-04-26 1995-11-09 Cadus Pharmaceutical Corporation Functional expression of mammalian adenylyl cyclase in yeast

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
ARKINSTALL, S. ET AL.: "Activation of Phospholipase Cgamma in Schizosaccharomyces pombe by Coexpression of Receptor or Nonreceptor Tyrosine Kinase", MOLECULAR AND CELLULAR BIOLOGY, vol. 15, no. 3, March 1995 (1995-03-01), pages 1431 - 1438, XP000609196 *
ARKINSTALL, S. ET AL.: "Co-Expression of the Neurokinin NK2 Receptor and G-Protein Components in the Fission Yeast Schizosaccharomyces pombe", FEBS LETTERS, vol. 375, November 1995 (1995-11-01), pages 183 - 187, XP002018654 *
ARKINSTALL, S. ET AL.: "Mapping Regions of Galphaq Interacting with PLCbeta1 Using Multiple Overlapping Synthetic Peptides", FEBS LETTERS, vol. 364, May 1995 (1995-05-01), pages 45 - 50, XP002018653 *
BOYER, J. ET AL.: "G-Protein-Mediated Regulation of Phospholipase C - In volvement of beta / gamma Subunits", TCM, vol. 4, no. 2, 1994, pages 88 - 95, XP000611204 *
FLICK, J. S. AND THORNER, J.: "Genetic and Biochemical Characterization of a Phosphatidylinositol-Specific Phospholipase C in Saccharomyces cerevisiae", MOLECULAR AND CELLULAR BIOLOGY, vol. 13, no. 9, September 1993 (1993-09-01), pages 5861 - 5876, XP000609195 *
MELDRUM, E. ET AL.: "The PtdIns-PLC Superfamily and Signal Transduction", BIOCHIMICA AND BIOPHYSICA ACTA, vol. 1092, 1991, pages 49 - 71, XP000611209 *
YOKO-O, T. ET AL.: "The Putative Phosphoinositide-Specific Phospholipase C Gene, PLC1, of the Yeast Saccharomyces cerevisiae is important for Cell Growth", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 90, March 1993 (1993-03-01), pages 1804 - 1808, XP002018652 *

Also Published As

Publication number Publication date
JP2001507201A (en) 2001-06-05
CA2220459A1 (en) 1996-12-19
WO1996040939A2 (en) 1996-12-19
AU6110896A (en) 1996-12-30
EP0832248A2 (en) 1998-04-01

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