WO1996041015A3 - Enhancement of alkaline phosphatase with sds in chemiluminescent substrates and enzyme inhibition assays - Google Patents

Enhancement of alkaline phosphatase with sds in chemiluminescent substrates and enzyme inhibition assays Download PDF

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Publication number
WO1996041015A3
WO1996041015A3 PCT/US1996/009767 US9609767W WO9641015A3 WO 1996041015 A3 WO1996041015 A3 WO 1996041015A3 US 9609767 W US9609767 W US 9609767W WO 9641015 A3 WO9641015 A3 WO 9641015A3
Authority
WO
WIPO (PCT)
Prior art keywords
alkaline phosphatase
sds
enhancement
enzyme inhibition
inhibition assays
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1996/009767
Other languages
French (fr)
Other versions
WO1996041015A2 (en
WO1996041015A9 (en
Inventor
Patrick J Sheridan
Julio C Gagne
Mary L Anderson
Douglas N Ludtke
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis Vaccines and Diagnostics Inc
Original Assignee
Chiron Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/472,756 external-priority patent/US5780227A/en
Priority to DE69635109T priority Critical patent/DE69635109T2/en
Priority to AT96921438T priority patent/ATE302856T1/en
Priority to AU62663/96A priority patent/AU716770B2/en
Priority to CA2221509A priority patent/CA2221509C/en
Priority to JP9501991A priority patent/JPH11507531A/en
Application filed by Chiron Corp filed Critical Chiron Corp
Priority to EP96921438A priority patent/EP0832296B1/en
Publication of WO1996041015A2 publication Critical patent/WO1996041015A2/en
Publication of WO1996041015A9 publication Critical patent/WO1996041015A9/en
Publication of WO1996041015A3 publication Critical patent/WO1996041015A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

A method of preparing a homogeneous alkaline phosphatase-oligonucleotide probe conjugate having high specific enzyme activity for use in nucleic acid hybridization assays is disclosed. Methods and compositions for enhancing the chemiluminescence from a stable 1,2-dioxetane triggered to produce a chemiluminescence are also disclosed. Indirect, competitive nucleic acid hybridization assay formats are also described that employ these methods and compositions.
PCT/US1996/009767 1995-06-07 1996-06-07 Enhancement of alkaline phosphatase with sds in chemiluminescent substrates and enzyme inhibition assays Ceased WO1996041015A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP96921438A EP0832296B1 (en) 1995-06-07 1996-06-07 Enhancement of alkaline phosphatase with sds in chemiluminescent substrates and enzyme inhibition assays
AT96921438T ATE302856T1 (en) 1995-06-07 1996-06-07 ENHANCEMENT OF ALKALINE PHOSPHATASE USING SDS AS A CHEMILUMINESE SUBSTRATE AND METHOD FOR ENZYME INHIBITION
AU62663/96A AU716770B2 (en) 1995-06-07 1996-06-07 Enhancement of alkaline phosphatase with SDS in chemiluminescent substrates and enzyme inhibition assays
CA2221509A CA2221509C (en) 1995-06-07 1996-06-07 Enhancement of alkaline phosphatase with sds in chemiluminescent substrates and enzyme inhibition assays
JP9501991A JPH11507531A (en) 1995-06-07 1996-06-07 Enhanced alkaline phosphatase and enzyme inhibition assays using SDS in chemiluminescent substrates
DE69635109T DE69635109T2 (en) 1995-06-07 1996-06-07 REINFORCEMENT OF ALKALINE PHOSPHATASE BY SDS AS A CHEMILUMINESUBSTRATE AND METHOD FOR ENZYMIN HIBITION

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US08/472,756 1995-06-07
US08/472,756 US5780227A (en) 1995-06-07 1995-06-07 Oligonucleotide probe conjugated to a purified hydrophilic alkaline phosphatase and uses thereof
US08/610,955 US5853974A (en) 1995-06-07 1996-03-05 Enhancement of alkaline phosphatase with SDS in chemiluminescent substrates
US08/610,955 1996-03-05

Publications (3)

Publication Number Publication Date
WO1996041015A2 WO1996041015A2 (en) 1996-12-19
WO1996041015A9 WO1996041015A9 (en) 1997-02-20
WO1996041015A3 true WO1996041015A3 (en) 1997-05-01

Family

ID=27043908

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1996/009767 Ceased WO1996041015A2 (en) 1995-06-07 1996-06-07 Enhancement of alkaline phosphatase with sds in chemiluminescent substrates and enzyme inhibition assays

Country Status (8)

Country Link
US (1) US5853974A (en)
EP (2) EP0832296B1 (en)
JP (1) JPH11507531A (en)
AT (2) ATE302856T1 (en)
AU (1) AU716770B2 (en)
DE (2) DE69637766D1 (en)
ES (2) ES2315769T3 (en)
WO (1) WO1996041015A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9622524D0 (en) 1996-10-29 1997-01-08 London Biotechnology Ltd Enzyme labels for assays
US6783965B1 (en) 2000-02-10 2004-08-31 Mountain View Pharmaceuticals, Inc. Aggregate-free urate oxidase for preparation of non-immunogenic polymer conjugates
ATE440964T1 (en) * 2000-03-23 2009-09-15 Lumigen Inc METHOD FOR DETECTING POLYNUCLEOTIDE KINASE AND USE THEREOF AS A MARKER
JP4576752B2 (en) * 2001-05-29 2010-11-10 富士レビオ株式会社 Chemiluminescence enhancer
US20040018495A1 (en) * 2002-07-24 2004-01-29 Xing-Xiang Li Microparticle based signal amplification for the detection of analytes
JP5414424B2 (en) * 2009-08-31 2014-02-12 シスメックス株式会社 Method for detecting target substance and chromatographic test kit for detecting target substance
CN113008879B (en) * 2021-02-25 2023-05-09 陕西科技大学 A method for detecting alkaline phosphatase using inverse opal hydrogel film

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0225807A2 (en) * 1985-12-11 1987-06-16 Chiron Corporation Solution phase nucleic acid sandwich assay and polynucleotide probes useful therein
EP0254051A2 (en) * 1986-07-17 1988-01-27 The Board Of Governors Of Wayne State University Chemiluminescent 1,2-dioxetane compounds
EP0317077A1 (en) * 1987-10-15 1989-05-14 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
EP0417841A2 (en) * 1989-09-12 1991-03-20 Eastman Kodak Company An oligonucleotide-enzyme conjugate and a method for its preparation
WO1993013221A1 (en) * 1991-12-23 1993-07-08 Chiron Corporation Chlamydiae probes for use in solution phase sandwich hybridization assays
US5254469A (en) * 1989-09-12 1993-10-19 Eastman Kodak Company Oligonucleotide-enzyme conjugate that can be used as a probe in hybridization assays and polymerase chain reaction procedures

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1260372A (en) * 1984-04-27 1989-09-26 Elazar Rabbani Hybridization method for the detection of genetic materials
US4978614A (en) * 1988-10-26 1990-12-18 Tropix, Inc. Method of detecting a substance using enzymatically-induced decomposition of dioxetanes
US5112960A (en) * 1989-07-17 1992-05-12 Bronstein Irena Y Chemiluminescent 3-(substituted adamant-2'-ylidene) 1,2-dioxetanes
CA1340590C (en) * 1986-07-24 1999-06-08 John C. Voyta Chemiluminescence enhancement
ZA877772B (en) * 1986-10-23 1988-04-20 Amoco Corporation Target and background capture methods and apparatus for affinity assays
US5124246A (en) * 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
US5094939A (en) * 1988-07-19 1992-03-10 Fujirebio, Inc. Chemiluminescence assays using stabilized dioxetane derivatives
CA2025178A1 (en) * 1989-10-02 1991-04-03 William G. Weisburg Nucleic acid probes for the detection of lyme disease spirochetes
AU7953091A (en) 1990-06-27 1992-01-23 Wilhelm Ernst Bucket with means for increasing its stability
US5474895A (en) * 1990-11-14 1995-12-12 Siska Diagnostics Inc. Non-isotopic detection of nucleic acids using a polystyrene support-based sandwich hybridization assay and compositions useful therefor
JPH0579985A (en) * 1991-09-24 1993-03-30 Kyoto Daiichi Kagaku:Kk Chemical luminescence reinforcing agent and reinforcing method
EP0625214A4 (en) * 1991-12-23 1997-07-16 Chiron Corp Cmv probes for use in solution phase sandwich hybridization assays.
WO1993013223A1 (en) * 1991-12-23 1993-07-08 Chiron Corporation Hiv probes for use in solution phase sandwich hybridization assays
US5747244A (en) 1991-12-23 1998-05-05 Chiron Corporation Nucleic acid probes immobilized on polystyrene surfaces
CA2119701A1 (en) * 1993-04-16 1994-10-17 Colleen M. Nycz Nucleic acid assay procedure
US5451347A (en) * 1993-06-24 1995-09-19 Lumigen, Inc. Methods and compositions providing enhanced chemiluminescence from chemiluminescent compounds using dicationic surfactants
US5578253A (en) * 1994-11-23 1996-11-26 Lumigen, Inc. Chemiluminescent dialkyl-substituted 1,2-dioxetane compounds, methods of synthesis and use
US5747248A (en) 1994-12-05 1998-05-05 Chiron Corporation Discontinuous probe design using hybritope mapping

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0225807A2 (en) * 1985-12-11 1987-06-16 Chiron Corporation Solution phase nucleic acid sandwich assay and polynucleotide probes useful therein
EP0254051A2 (en) * 1986-07-17 1988-01-27 The Board Of Governors Of Wayne State University Chemiluminescent 1,2-dioxetane compounds
EP0317077A1 (en) * 1987-10-15 1989-05-14 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
EP0417841A2 (en) * 1989-09-12 1991-03-20 Eastman Kodak Company An oligonucleotide-enzyme conjugate and a method for its preparation
US5254469A (en) * 1989-09-12 1993-10-19 Eastman Kodak Company Oligonucleotide-enzyme conjugate that can be used as a probe in hybridization assays and polymerase chain reaction procedures
WO1993013221A1 (en) * 1991-12-23 1993-07-08 Chiron Corporation Chlamydiae probes for use in solution phase sandwich hybridization assays

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DAVINI E ET AL: "ALKALINE PHOSPHATASE INHIBITORS AS LABELS OF DNA PROBES", GENETIC ANALYSIS TECHNIQUES AND APPLICATIONS, vol. 9, no. 2, 1 April 1992 (1992-04-01), pages 39 - 47, XP000307396 *
R. BUBLITZ ET AL.: "Heterogeneity of glycosylphosphatidylinositol-anchored alkaline phosphatase", EUR. J. BIOCHEM., vol. 217, no. 1, October 1993 (1993-10-01), SPRINGER, BERLIN, D, pages 199 - 207, XP000612166 *

Also Published As

Publication number Publication date
EP0832296B1 (en) 2005-08-24
ES2247605T3 (en) 2006-03-01
AU716770B2 (en) 2000-03-09
EP1616966A2 (en) 2006-01-18
EP1616966B1 (en) 2008-11-26
DE69635109T2 (en) 2006-06-14
EP0832296A2 (en) 1998-04-01
ATE415494T1 (en) 2008-12-15
JPH11507531A (en) 1999-07-06
DE69637766D1 (en) 2009-01-08
ATE302856T1 (en) 2005-09-15
AU6266396A (en) 1996-12-30
EP1616966A3 (en) 2007-10-03
ES2315769T3 (en) 2009-04-01
WO1996041015A2 (en) 1996-12-19
DE69635109D1 (en) 2005-09-29
US5853974A (en) 1998-12-29

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