WO1997017457A3 - Stable packaging cell line producing pseudotyped retroviruses - Google Patents

Stable packaging cell line producing pseudotyped retroviruses Download PDF

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Publication number
WO1997017457A3
WO1997017457A3 PCT/US1996/017807 US9617807W WO9717457A3 WO 1997017457 A3 WO1997017457 A3 WO 1997017457A3 US 9617807 W US9617807 W US 9617807W WO 9717457 A3 WO9717457 A3 WO 9717457A3
Authority
WO
WIPO (PCT)
Prior art keywords
present
retroviral
expression
relates
packaging cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1996/017807
Other languages
French (fr)
Other versions
WO1997017457A2 (en
Inventor
Daniel S Ory
Michel Sadelain
Richard C Mulligan
Jean E Schaffer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Washington
Washington University in St Louis WUSTL
Whitehead Institute for Biomedical Research
Original Assignee
University of Washington
Washington University in St Louis WUSTL
Whitehead Institute for Biomedical Research
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Washington, Washington University in St Louis WUSTL, Whitehead Institute for Biomedical Research filed Critical University of Washington
Priority to EP96942729A priority Critical patent/EP0859856A2/en
Priority to JP9518303A priority patent/JP2000500013A/en
Publication of WO1997017457A2 publication Critical patent/WO1997017457A2/en
Publication of WO1997017457A3 publication Critical patent/WO1997017457A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13051—Methods of production or purification of viral material
    • C12N2740/13052—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to a stable, pseudotyped retrovirus packaging cell line comprising packaging cells which generate helper-free recombinant pseudotyped retrovirus. The packaging cell line comprises one or more non-retroviral expression constructs, such as an expression construct with the human cytomegalovirus (CMV) immediate early promoter or derivatives of this promoter (e.g., pMD), which direct expression of: (a) retroviral gagpol genes and (b) a non-retroviral gene which is under the control of an inducible operator system and whose gene product pseudotypes retroviral core virions. The present invention further relates to a method of making a stable, pseudotyped retrovirus packaging cell line which generates helper-free recombinant pseudotyped retrovirus. The present invention further relates to the particular packaging cell lines described herein (i.e., H29 gagpol, H29 new gagpol) and the particular cells and constructs (i.e., packaging elements) used to produce the stable, pseudotyped retrovirus packaging cell line described herein (e.g., H29 cells and pMD, pMDtet, pMDtet.G, PMD.gagpol, pMD.new gagpol constructs). The present invention relates to a retroviral vector for producing a cDNA library for expression in mammalian cells, comprising two retroviral long terminal repeats, a cloning site for insertion of cDNA and a cytomegalovirus promoter. The invention also relates to a cDNA library for expression in mammalian cells, the library comprising retroviral vectors of the present invention. The present invention also relates to a method of expression cloning in mammalian cells. The present invention also relates to a method of cDNA expression cloning in mammalian cells. The present invention also relates to a method of identifying a gene defect responsible for a mutant phenotype using cDNA expression cloning by complementation in mammalian cells.
PCT/US1996/017807 1995-11-08 1996-11-07 Stable packaging cell line producing pseudotyped retroviruses Ceased WO1997017457A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP96942729A EP0859856A2 (en) 1995-11-08 1996-11-07 Stable packaging cell line producing pseudotyped retroviruses
JP9518303A JP2000500013A (en) 1995-11-08 1996-11-07 Stable packaging cell line producing pseudotype retrovirus

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US55515595A 1995-11-08 1995-11-08
US65105096A 1996-05-21 1996-05-21
US08/651,050 1996-05-21
US08/555,155 1996-05-21

Publications (2)

Publication Number Publication Date
WO1997017457A2 WO1997017457A2 (en) 1997-05-15
WO1997017457A3 true WO1997017457A3 (en) 1997-09-12

Family

ID=27070812

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1996/017807 Ceased WO1997017457A2 (en) 1995-11-08 1996-11-07 Stable packaging cell line producing pseudotyped retroviruses

Country Status (4)

Country Link
EP (1) EP0859856A2 (en)
JP (1) JP2000500013A (en)
CA (1) CA2237000A1 (en)
WO (1) WO1997017457A2 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1717248A1 (en) 1997-06-04 2006-11-02 Oxford Biomedica (UK) Limited Tumor targeted vector
AU1334999A (en) * 1997-10-20 1999-05-10 Universita Degli Studi Di Padova A packaging cell line producing siv-pseudotyped mlv
PT1076715E (en) 1998-05-13 2007-10-26 Genetix Pharmaceuticals Inc Lentiviral packaging cells
GB0009760D0 (en) 2000-04-19 2000-06-07 Oxford Biomedica Ltd Method
GB0024550D0 (en) 2000-10-06 2000-11-22 Oxford Biomedica Ltd
CA2344208A1 (en) 2001-04-30 2002-10-30 Oxford Biomedica (Uk) Limited Method
AUPR518501A0 (en) 2001-05-22 2001-06-14 Unisearch Limited Yin yang-1
EP1504108B1 (en) 2002-02-01 2013-04-17 Oxford Biomedica (UK) Limited Lentiviral vector
EP2045268B1 (en) 2005-05-13 2011-09-21 Oxford BioMedica (UK) Limited Mhc class I and II peptide antigens derived from tumour antigen 5t4
WO2010085660A2 (en) 2009-01-23 2010-07-29 Roger Williams Hospital Viral vectors encoding multiple highly homologous non-viral polypeptides and the use of same
CN103328038A (en) 2010-12-01 2013-09-25 史拜诺莫度雷森公司 Directed delivery of agents to neural anatomy
GB201318804D0 (en) 2013-10-24 2013-12-11 Adaptimmune Ltd Vectors for transgene expression
DE102020111571A1 (en) 2020-03-11 2021-09-16 Immatics US, Inc. WPRE MUTANT CONSTRUCTS, COMPOSITIONS, AND RELATED PROCEDURES
DE102020106710A1 (en) 2020-03-11 2021-09-16 Immatics US, Inc. WPRE MUTANT CONSTRUCTS, COMPOSITIONS, AND RELATED PROCEDURES
WO2025240518A1 (en) 2024-05-14 2025-11-20 Dispatch Biotherapeutics, Inc. Modified lentiviral transfer plasmids

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994029440A1 (en) * 1993-06-04 1994-12-22 The Regents Of The University Of California Generation, concentration and efficient transfer of vsv-g pseudotyped retroviral vectors
WO1996004934A1 (en) * 1994-08-17 1996-02-22 Genetic Therapy Inc. Retroviral vectors produced by producer cell lines resistant to lysis by human serum
WO1996035454A1 (en) * 1995-05-08 1996-11-14 St. Jude Children's Research Hospital Stable virus packaging cell lines

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994029440A1 (en) * 1993-06-04 1994-12-22 The Regents Of The University Of California Generation, concentration and efficient transfer of vsv-g pseudotyped retroviral vectors
WO1996004934A1 (en) * 1994-08-17 1996-02-22 Genetic Therapy Inc. Retroviral vectors produced by producer cell lines resistant to lysis by human serum
WO1996035454A1 (en) * 1995-05-08 1996-11-14 St. Jude Children's Research Hospital Stable virus packaging cell lines

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
D.S. ORY ET AL.: "A stable human-derived packaging cell line for production of high titer retrovirus/vesicular stomatitis virus G pseudotypes", PROC. NATL. ACAD. SCI. USA, vol. 93, 1996, pages 11400 - 11406, XP002030784 *
J.-K. YEE ET AL.: "Generation of high-titer pseudotyped retroviral vectors with very broad host range", METHODS CELL BIOL., vol. 43, 1994, pages 99 - 112, XP000570503 *
Y. YANG ET AL.: "Inducible, high-level production of infectious murine leukemia retroviral vector particles pseudotyped with vesicular stomatitis virus G envelope protein", HUMAN GENE THERAPY, vol. 6, 1995, pages 1203 - 1213, XP000569741 *

Also Published As

Publication number Publication date
WO1997017457A2 (en) 1997-05-15
CA2237000A1 (en) 1997-05-15
EP0859856A2 (en) 1998-08-26
JP2000500013A (en) 2000-01-11

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