WO1997024452A3 - Toxic-protein expressing viruses and producer cell lines - Google Patents

Toxic-protein expressing viruses and producer cell lines Download PDF

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Publication number
WO1997024452A3
WO1997024452A3 PCT/US1996/020587 US9620587W WO9724452A3 WO 1997024452 A3 WO1997024452 A3 WO 1997024452A3 US 9620587 W US9620587 W US 9620587W WO 9724452 A3 WO9724452 A3 WO 9724452A3
Authority
WO
WIPO (PCT)
Prior art keywords
toxic
cell lines
producer cell
protein expressing
expressing viruses
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1996/020587
Other languages
French (fr)
Other versions
WO1997024452A2 (en
Inventor
Sunil Chada
Nicholas J Depolo
Sybille Sauter
Joan Robbins
Douglas J Jolly
William C Manning
John E Murphy
Robert O Ii Ralston
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis Vaccines and Diagnostics Inc
Original Assignee
Chiron Viagene Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chiron Viagene Inc filed Critical Chiron Viagene Inc
Priority to DK96945414T priority Critical patent/DK0870050T3/en
Priority to AT96945414T priority patent/ATE219521T1/en
Priority to EP96945414A priority patent/EP0870050B1/en
Priority to DE69621949T priority patent/DE69621949T2/en
Priority to JP09523909A priority patent/JP2000502261A/en
Publication of WO1997024452A2 publication Critical patent/WO1997024452A2/en
Publication of WO1997024452A3 publication Critical patent/WO1997024452A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/13043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/20011—Rhabdoviridae
    • C12N2760/20211—Vesiculovirus, e.g. vesicular stomatitis Indiana virus
    • C12N2760/20222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
    • C12N2830/003—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor tet inducible
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/10—Vectors comprising a special translation-regulating system regulates levels of translation
    • C12N2840/105—Vectors comprising a special translation-regulating system regulates levels of translation enhancing translation

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Expression vectors are provided which have a tightly controllable promoter which directs the expression of a fusogenic protein. Also provided are nucleic acid molecules which encode toxic of fusogenic proteins, wherein one or more cysteine residues in the nucleic acid molecule has been altered to an opal termination codon.
PCT/US1996/020587 1995-12-29 1996-12-20 Toxic-protein expressing viruses and producer cell lines Ceased WO1997024452A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
DK96945414T DK0870050T3 (en) 1995-12-29 1996-12-20 Virus expressing a toxic protein and producer cell lines
AT96945414T ATE219521T1 (en) 1995-12-29 1996-12-20 TOXIC PROTEIN EXPRESSING VIRUSES AND PRODUCTION CELL LINES
EP96945414A EP0870050B1 (en) 1995-12-29 1996-12-20 Toxic-protein expressing viruses and producer cell lines
DE69621949T DE69621949T2 (en) 1995-12-29 1996-12-20 TOXIC PROTEIN-EXPRESSING VIRUSES AND PRODUCTION CELL LINES
JP09523909A JP2000502261A (en) 1995-12-29 1996-12-20 Toxic protein expressing viruses and producer cell lines

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US58055695A 1995-12-29 1995-12-29
US58055495A 1995-12-29 1995-12-29
US08/580,554 1995-12-29
US08/580,556 1995-12-29

Publications (2)

Publication Number Publication Date
WO1997024452A2 WO1997024452A2 (en) 1997-07-10
WO1997024452A3 true WO1997024452A3 (en) 1997-11-06

Family

ID=27078078

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1996/020587 Ceased WO1997024452A2 (en) 1995-12-29 1996-12-20 Toxic-protein expressing viruses and producer cell lines

Country Status (8)

Country Link
EP (1) EP0870050B1 (en)
JP (1) JP2000502261A (en)
AT (1) ATE219521T1 (en)
DE (1) DE69621949T2 (en)
DK (1) DK0870050T3 (en)
ES (1) ES2175184T3 (en)
PT (1) PT870050E (en)
WO (1) WO1997024452A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2337520B (en) * 1998-05-22 2000-06-14 Oxford Biomedica Ltd Delivery system
KR20010079671A (en) * 1998-08-20 2001-08-22 스티븐 에이. 서윈. 엠.디. USE OF SUPPRESSOR tRNA'S TO REGULATE CYTOTOXICITY DURING THE PRODUCTION OF RECOMBINANT GENE PRODUCTS

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987003905A1 (en) * 1985-12-18 1987-07-02 Genetics Institute, Inc. Promoter system
WO1994029440A1 (en) * 1993-06-04 1994-12-22 The Regents Of The University Of California Generation, concentration and efficient transfer of vsv-g pseudotyped retroviral vectors

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987003905A1 (en) * 1985-12-18 1987-07-02 Genetics Institute, Inc. Promoter system
WO1994029440A1 (en) * 1993-06-04 1994-12-22 The Regents Of The University Of California Generation, concentration and efficient transfer of vsv-g pseudotyped retroviral vectors

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DANIEL S. ORY ET AL.: "A stable human-derived packaging cell line for production of high titer retrovirus/vesicular stomatitis virus G pseudotypes", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 93, no. 21, 15 October 1996 (1996-10-15), WASHINGTON US, pages 11400 - 11406, XP002030515 *
MANFRED SCHUBERT ET AL.: "Insertion of the Human Immunodeficiency Virus CD4 receptor into the envelope of Vesicular Stomatitis Virus particles", JOURNAL OF VIROLOGY, vol. 66, no. 3, March 1992 (1992-03-01), pages 1579 - 1589, XP000644669 *

Also Published As

Publication number Publication date
JP2000502261A (en) 2000-02-29
EP0870050B1 (en) 2002-06-19
ATE219521T1 (en) 2002-07-15
EP0870050A2 (en) 1998-10-14
PT870050E (en) 2002-10-31
DE69621949D1 (en) 2002-08-01
WO1997024452A2 (en) 1997-07-10
DE69621949T2 (en) 2002-09-26
DK0870050T3 (en) 2002-07-15
ES2175184T3 (en) 2002-11-16

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