WO1997026270A3 - Synthesis of methoxy nucleosides and enzymatic nucleic acid molecules - Google Patents

Synthesis of methoxy nucleosides and enzymatic nucleic acid molecules Download PDF

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Publication number
WO1997026270A3
WO1997026270A3 PCT/US1996/020527 US9620527W WO9726270A3 WO 1997026270 A3 WO1997026270 A3 WO 1997026270A3 US 9620527 W US9620527 W US 9620527W WO 9726270 A3 WO9726270 A3 WO 9726270A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
synthesis
acid molecules
enzymatic nucleic
nucleosides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1996/020527
Other languages
French (fr)
Other versions
WO1997026270A2 (en
Inventor
Francine Wincott
Nassim Usman
Leonid Beigelman
Peter Haeberli
Jasenka Matulic-Adamic
Alexander Karpeisky
David Sweedler
Thale Jarvis
Anthony Direnzo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sirna Therapeutics Inc
Original Assignee
Ribozyme Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/585,682 external-priority patent/US5728818A/en
Priority claimed from US08/632,882 external-priority patent/US5998203A/en
Application filed by Ribozyme Pharmaceuticals Inc filed Critical Ribozyme Pharmaceuticals Inc
Priority to AU14300/97A priority Critical patent/AU1430097A/en
Priority to CA002253382A priority patent/CA2253382A1/en
Priority to EP96944523A priority patent/EP0886641A2/en
Publication of WO1997026270A2 publication Critical patent/WO1997026270A2/en
Publication of WO1997026270A3 publication Critical patent/WO1997026270A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/121—Hammerhead

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

This invention relates to chemical synthesis of 2'-O-methyl, 3'-O-methyl and 5'-O-methyl nucleosides, incorporation of novel chemical modifications in enzymatic nucleic acid molecules and improved methods for the synthesis of enzymatic nucleic acid molecules.
PCT/US1996/020527 1996-01-16 1996-12-23 Synthesis of methoxy nucleosides and enzymatic nucleic acid molecules Ceased WO1997026270A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU14300/97A AU1430097A (en) 1996-01-16 1996-12-23 Synthesis of methoxy nucleosides and enzymatic nucleic acid molecules
CA002253382A CA2253382A1 (en) 1996-01-16 1996-12-23 Synthesis of methoxy nucleosides and enzymatic nucleic acid molecules
EP96944523A EP0886641A2 (en) 1996-01-16 1996-12-23 Synthesis of methoxy nucleosides and enzymatic nucleic acid molecules

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US08/585,682 1996-01-16
US08/585,682 US5728818A (en) 1996-01-16 1996-01-16 Chemical linkage of ribozyme protions
US60042996A 1996-02-13 1996-02-13
US08/600,429 1996-02-13
US08/632,882 1996-04-16
US08/632,882 US5998203A (en) 1996-04-16 1996-04-16 Enzymatic nucleic acids containing 5'-and/or 3'-cap structures

Publications (2)

Publication Number Publication Date
WO1997026270A2 WO1997026270A2 (en) 1997-07-24
WO1997026270A3 true WO1997026270A3 (en) 1997-11-20

Family

ID=27416459

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1996/020527 Ceased WO1997026270A2 (en) 1996-01-16 1996-12-23 Synthesis of methoxy nucleosides and enzymatic nucleic acid molecules

Country Status (6)

Country Link
EP (2) EP1108724B1 (en)
AT (1) ATE373672T1 (en)
AU (1) AU1430097A (en)
CA (1) CA2253382A1 (en)
DE (1) DE69637256T2 (en)
WO (1) WO1997026270A2 (en)

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US9150606B2 (en) 2002-11-05 2015-10-06 Isis Pharmaceuticals, Inc. Compositions comprising alternating 2'-modified nucleosides for use in gene modulation
US9394330B2 (en) 2012-03-21 2016-07-19 Alios Biopharma, Inc. Solid forms of a thiophosphoramidate nucleotide prodrug
US11932854B2 (en) 2010-10-29 2024-03-19 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using short interfering nucleic acids (siNA)

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CA2253382A1 (en) 1997-07-24
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