WO1997042327A3 - MÉTODO PARA INCREMENTAR EL CONTENIDO DE TREHALOSA DE LOS ORGANISMOS POR MEDIO DE SU TRANSFORMACIÓN CON EL ADNc DE LA TREHALOSA-6-FOSFATO SINTASA/FOSFATASA DE SELAGINELLA LEPIDOPHYLLA - Google Patents

MÉTODO PARA INCREMENTAR EL CONTENIDO DE TREHALOSA DE LOS ORGANISMOS POR MEDIO DE SU TRANSFORMACIÓN CON EL ADNc DE LA TREHALOSA-6-FOSFATO SINTASA/FOSFATASA DE SELAGINELLA LEPIDOPHYLLA Download PDF

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Publication number
WO1997042327A3
WO1997042327A3 PCT/MX1997/000012 MX9700012W WO9742327A3 WO 1997042327 A3 WO1997042327 A3 WO 1997042327A3 MX 9700012 W MX9700012 W MX 9700012W WO 9742327 A3 WO9742327 A3 WO 9742327A3
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WIPO (PCT)
Prior art keywords
trehalose
complementary dna
plants
phosphatase
increasing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/MX1997/000012
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English (en)
French (fr)
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WO1997042327A2 (es
Inventor
De La Fuente Gabriel Iturriaga
Gomez Rodolfo Zentella
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Universidad Nacional Autonoma de Mexico
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Universidad Nacional Autonoma de Mexico
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Publication date
Application filed by Universidad Nacional Autonoma de Mexico filed Critical Universidad Nacional Autonoma de Mexico
Priority to AU29135/97A priority Critical patent/AU727509B2/en
Priority to BR9710436A priority patent/BR9710436A/pt
Priority to EP97923309A priority patent/EP0933428A2/en
Priority to JP9539760A priority patent/JP2000509602A/ja
Publication of WO1997042327A2 publication Critical patent/WO1997042327A2/es
Publication of WO1997042327A3 publication Critical patent/WO1997042327A3/es
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Nutrition Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • General Preparation And Processing Of Foods (AREA)
  • Seasonings (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)

Abstract

Se clonó y determinó la secuencia nucleotídica de una molécula completa de ADN complementario que codifica para la enzima bifuncional trehalosa-6-fosfato sintasa/fosfatasa de plantas. La molécula de ADN complementario codifica para una enzima bifuncional que sintetiza trehalosa. El ADN complementario se puede subclonar en vectores apropiados para su expresión en células huéspedes. Las células transformadas producen trehalosa, en contraste con las células no transformadas, lo cual aumentará la tolerancia al estrés por calor, frío, salinidad y sequía. Esta invención se podrá usar para mejorar la termotolerancia y osmotolerancia de las plantas cultivadas en zonas áridas o semiáridas y también contribuirá a disminuir el uso de agua de riego. Por otro lado, la trehalosa producida en plantas transgénicas podría incrementar la vida de anaquel de los productos agrícolas, preservándolos por períodos largos en estado deshidratado sin que estos pierdan sus propiedades de olor, sabor y textura, una vez que sean rehidratados. Por último, la sobreproducción de trehalosa en bacterias, levaduras, hongos, células animales o plantas transgénicas constituirá una fuente barata de este producto para ser usado como aditivo para preservar diversos productos biológicos o alimentos procesados.
PCT/MX1997/000012 1996-05-08 1997-05-06 MÉTODO PARA INCREMENTAR EL CONTENIDO DE TREHALOSA DE LOS ORGANISMOS POR MEDIO DE SU TRANSFORMACIÓN CON EL ADNc DE LA TREHALOSA-6-FOSFATO SINTASA/FOSFATASA DE SELAGINELLA LEPIDOPHYLLA Ceased WO1997042327A2 (es)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU29135/97A AU727509B2 (en) 1996-05-08 1997-05-06 Method for increasing the trehalose content in organisms by means of their transformation with the cDNA of trehalose- 6-phosphate synthase/phosphatase from selaginella lepidophylla
BR9710436A BR9710436A (pt) 1996-05-08 1997-05-06 Processo para aumentar o teor de trealose em organismos por meio de sua transforma-Æo com o cdna da sintese-fosfatase de trealose-6-fosfato de selaginella lepidophylla
EP97923309A EP0933428A2 (en) 1996-05-08 1997-05-06 METHOD FOR INCREASING THE CONTENT OF TREHALOSE IN ORGANISMS THROUGH THE TRANSFORMATION THEREOF WITH THE cDNA OF THE TREHALOSE-6-PHOSPHATE SYNTHETASE/PHOSPHATASE OF SELAGINELLA LEPIDOPHYLLA
JP9539760A JP2000509602A (ja) 1996-05-08 1997-05-06 セラギネラ・レピドフィラ由来のトレハロース―６―リン酸シンターゼ／ホスファターゼのｃＤＮＡで形質転換することによる、生物中のトレハロース含量の増加法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
MX9601719A MX205414B (es) 1996-05-08 1996-05-08 Metodo para incrementar de trehalosa de los organismos por medio de su transformacion con el adnc de la trehalosa-6-fosfato sintasa/fosfatasa de selaginella lepidophylla.
MX961719 1996-05-08

Publications (2)

Publication Number Publication Date
WO1997042327A2 WO1997042327A2 (es) 1997-11-13
WO1997042327A3 true WO1997042327A3 (es) 1998-01-08

Family

ID=19744918

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/MX1997/000012 Ceased WO1997042327A2 (es) 1996-05-08 1997-05-06 MÉTODO PARA INCREMENTAR EL CONTENIDO DE TREHALOSA DE LOS ORGANISMOS POR MEDIO DE SU TRANSFORMACIÓN CON EL ADNc DE LA TREHALOSA-6-FOSFATO SINTASA/FOSFATASA DE SELAGINELLA LEPIDOPHYLLA

Country Status (6)

Country Link
EP (1) EP0933428A2 (es)
JP (1) JP2000509602A (es)
AU (1) AU727509B2 (es)
BR (1) BR9710436A (es)
MX (1) MX205414B (es)
WO (1) WO1997042327A2 (es)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IN1997CH00924A (en) 1996-05-03 2005-03-04 Syngenta Mogen Bv Regulating metabolism by modifying the level of trehalose-6-phosphate
TW466116B (en) 1997-03-04 2001-12-01 Hayashibara Biochem Lab Reduction inhibitory agent for active-oxygen eliminating activity, method for inhibiting the reduction of said activity, and composition containing said agent
WO1999023225A1 (en) * 1997-10-30 1999-05-14 Mogen International N.V. Novel high-fermenting microorganisms
WO1999024558A2 (en) * 1997-10-30 1999-05-20 Mogen International N.V. Novel high-fermenting microorganisms
HUP0102598A3 (en) * 1998-03-11 2003-03-28 Syngenta Participations Ag Expression of trehalose biosynthetic genes in plants
US7345217B2 (en) 1998-09-22 2008-03-18 Mendel Biotechnology, Inc. Polynucleotides and polypeptides in plants
EP1002867A1 (en) 1998-10-15 2000-05-24 K.U. Leuven Research & Development Specific genetic modification of the activity of trehalose-6-phosphate synthase and expression in a homologous or heterologous environment
MXPA02004870A (es) * 1999-11-17 2004-09-10 Mendel Biotechnology Inc Genes de tolerancia al estres ambiental.
EP2169069A3 (en) * 2000-04-07 2010-07-14 BASF Plant Science GmbH Phosphatase stress-related proteins and methods of use in plants
US8426678B2 (en) 2002-09-18 2013-04-23 Mendel Biotechnology, Inc. Polynucleotides and polypeptides in plants
EP1375669A1 (en) * 2002-06-13 2004-01-02 Stichting Voor De Technische Wetenschappen Method for enhancing the disease resistance in plants by altering trehalose-6-phosphate levels
EP1873247A1 (en) * 2006-06-29 2008-01-02 VIB vzw Novel bifunctional trehalose synthase
CL2009000440A1 (es) 2008-02-29 2010-11-12 Monsanto Technology Llc Polinucleotidos que comprende un promotor de actina de arroz truncado ligado operativamente al gen cspb, cromosoma de maiz que comprende dicho policleotido, procedimiemto para la generacion de especies vegetales resisentes a la sequia, y alimento basado en semillas de dichas especies vegetales.
CN103739548B (zh) * 2013-12-27 2015-08-05 南京中医药大学 具有抗真菌活性的化合物及其制备方法与应用

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0451896A1 (en) * 1990-03-28 1991-10-16 Gist-Brocades N.V. New yeast strains with enhanced trehalose content, process to obtain such yeasts and the use of these yeasts
WO1993017093A2 (en) * 1992-02-14 1993-09-02 Oy Alko Ab Increasing the trehalose content of organisms by transforming them with combinations of the structural genes for trehalose synthase
EP0577915A1 (fr) * 1992-07-09 1994-01-12 N.V. Algist-Bruggeman Souches de levures transformées de manière à posséder une résistance au stress et/ou un pouvoir fermentatif amélioré
WO1995001446A1 (en) * 1993-06-30 1995-01-12 Mogen International N.V. Production of trehalose in plants
WO1996000789A1 (en) * 1994-06-29 1996-01-11 Alko Group Limited Transgenic plants producing trehalose
WO1996021030A1 (en) * 1995-01-04 1996-07-11 Mogen International N.V. Enhanced accumulation of trehalose in plants
EP0784095A2 (en) * 1996-01-12 1997-07-16 Mogen International N.V. Enhanced accummulation of trehalose in plants

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IN1997CH00924A (en) * 1996-05-03 2005-03-04 Syngenta Mogen Bv Regulating metabolism by modifying the level of trehalose-6-phosphate

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0451896A1 (en) * 1990-03-28 1991-10-16 Gist-Brocades N.V. New yeast strains with enhanced trehalose content, process to obtain such yeasts and the use of these yeasts
WO1993017093A2 (en) * 1992-02-14 1993-09-02 Oy Alko Ab Increasing the trehalose content of organisms by transforming them with combinations of the structural genes for trehalose synthase
EP0577915A1 (fr) * 1992-07-09 1994-01-12 N.V. Algist-Bruggeman Souches de levures transformées de manière à posséder une résistance au stress et/ou un pouvoir fermentatif amélioré
WO1995001446A1 (en) * 1993-06-30 1995-01-12 Mogen International N.V. Production of trehalose in plants
WO1996000789A1 (en) * 1994-06-29 1996-01-11 Alko Group Limited Transgenic plants producing trehalose
WO1996021030A1 (en) * 1995-01-04 1996-07-11 Mogen International N.V. Enhanced accumulation of trehalose in plants
EP0784095A2 (en) * 1996-01-12 1997-07-16 Mogen International N.V. Enhanced accummulation of trehalose in plants

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
ADAMS R P ET AL: "COMPARISON OF FREE SUGARS IN GROWING AND DESICCATED PLANTS OF SELAGINELLA LEPIDOPHYLLA", BIOCHEMICAL SYSTEMATICS AND ECOLOGY, vol. 18, no. 2/03, 1 January 1990 (1990-01-01), pages 107 - 110, XP000567557 *
GANCEDO C.: "A thaliana mRNA for trehalose-6-phosphate synthase", EMBL SEQUENCE DATABASE, REL. 51, ACCESSION NO. Y08568, XP002044599 *
GODDIJN O J M ET AL: "TRANSGENIC TOBACCO PLANTS AS A MODEL-SYSTEM FOR THE PRODUCTION OF TREHALOSE", PLANT PHYSIOLOGY, vol. 108, no. 2, 1 June 1995 (1995-06-01), pages 149, XP002000477 *
HOLMSTRÖM, K.-O., ET AL.: "Drought tolerance in tobacco", NATURE, vol. 379, February 1996 (1996-02-01), pages 683 - 684, XP002043918 *
PALVA, E. TAPIO ET AL: "Enhanced desiccation survival by engineering osmolyte biosynthesis in plants", PHYS. STRESSES PLANTS: GENES THEIR PROD. TOLERANCE, PROC. WORKSHOP (1996), MEETING DATE 1995, 187-198. EDITOR(S): GRILLO, STEFANIA;LEONE, ANTONELLA. PUBLISHER: SPRINGER, BERLIN, GERMANY. CODEN: 64JRAU, XP002044597 *
PLANT BIOLOGY 1996, MEETING HELD JULY 27 - AUGUST 2, 1996. *
ROSER, B., ET AL.: "A sweeter way to fresher food", NEW SCIENTIST, vol. 134, 1993, pages 24 - 28, XP002044603 *
ZENTELLA, R., ET AL.: "Molecular characterization of a cDNA encoding trehalose-6-phosphate synthase/phosphatase from the resurrection plant Selaginella lepidophylla", PLANT PHYSIOLOGY SUPPLEMENT, vol. 111, no. 2, June 1996 (1996-06-01), pages 47, XP002044598 *

Also Published As

Publication number Publication date
AU727509B2 (en) 2000-12-14
BR9710436A (pt) 1999-08-17
MX205414B (es) 2001-12-07
WO1997042327A2 (es) 1997-11-13
EP0933428A2 (en) 1999-08-04
JP2000509602A (ja) 2000-08-02
AU2913597A (en) 1997-11-26
MX9601719A (es) 1997-11-29

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