WO1997044005A1 - Lecithin-based microemulsions containing proteolytic enzymes and method for permanent enzymic depilation - Google Patents

Lecithin-based microemulsions containing proteolytic enzymes and method for permanent enzymic depilation Download PDF

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Publication number
WO1997044005A1
WO1997044005A1 PCT/GR1997/000015 GR9700015W WO9744005A1 WO 1997044005 A1 WO1997044005 A1 WO 1997044005A1 GR 9700015 W GR9700015 W GR 9700015W WO 9744005 A1 WO9744005 A1 WO 9744005A1
Authority
WO
WIPO (PCT)
Prior art keywords
enzyme
microemulsion
lecithin
depilatory
depilation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GR1997/000015
Other languages
French (fr)
Inventor
Evangelia Protopapa
Aristotelis Xenakis
Spyridon Avramiotis
Konstantinos Sekeris
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US09/180,904 priority Critical patent/US6203791B1/en
Priority to DE69719559T priority patent/DE69719559T2/en
Priority to AU27098/97A priority patent/AU733595B2/en
Priority to BR9709341-6A priority patent/BR9709341A/en
Priority to EP97920886A priority patent/EP0955991B1/en
Priority to PL97330602A priority patent/PL187389B1/en
Priority to CA002255649A priority patent/CA2255649C/en
Priority to AT97920886T priority patent/ATE233537T1/en
Priority to IL12715097A priority patent/IL127150A/en
Priority to APAP/P/1998/001405A priority patent/AP1077A/en
Priority to NZ332898A priority patent/NZ332898A/en
Priority to DK97920886T priority patent/DK0955991T3/en
Application filed by Individual filed Critical Individual
Priority to SK1592-98A priority patent/SK281923B6/en
Publication of WO1997044005A1 publication Critical patent/WO1997044005A1/en
Priority to BG102938A priority patent/BG63658B1/en
Priority to IS4899A priority patent/IS2106B/en
Anticipated expiration legal-status Critical
Priority to NO985439A priority patent/NO985439D0/en
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/66—Enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q9/00—Preparations for removing hair or for aiding hair removal
    • A61Q9/04—Depilatories

Definitions

  • the present invention refers to the depilatory preparations of lecithin-based microemulsions containing proteolytic enzymes
  • the proposed invention introduces for
  • microemulsions as media for the soubilization of proteolytic enzymic systems, that assist the penetration of active molecules through the
  • the present invention refers to a depilation method, that applies the preparations of microemulsion containing the enzyme ⁇ - chymotrypsin, or the enzyme trypsin, for the depilation of any type of skin, as well as to
  • microemulsion containing the enzyme ⁇ -chymotrypsin followed by the application of the
  • Microemulsions are systems consisting of a continuous inert organic medium, where water droplets are dispersed with the help of lecithin acting as a natural surfactant
  • the dispersion of the aqueous phase, where the enzyme molecules ⁇ -chymotrypsin or trypsin are located, is achieved by simple and gentle shaking
  • Our invention improves the depilatory action of the enzymes ⁇ -chymotrypsin and trypsin, because of the fact that they are applied through the microemulsion The shape
  • microemulsion is such that allows its easy spreading over the whole surface of the microemulsion
  • the presence of the microemulsion offers the facility to be applied on every type of skin, such as the sensitive-dry ones, or the resistant-fatty ones
  • microemulsion containing the enzyme ⁇ -chymotrypsin followed by the application of the preparation of microemulsion containing the enzyme trypsin, provokes important degradation of the hair follicle, and that the sequential application first of the preparation
  • microemulsion containing the enzyme ⁇ -chymotrypsin and then of the preparation of
  • microemulsion containing the enzyme trypsin has better depilatory results than the
  • microemulsions as a carrier for the transdermal transfer of active
  • proteolytic enzymes ⁇ -chymotrypsin and trypsin will be used in
  • the enzymes will be stored lyophilized in a freezer (-18 °C) so that they are kept constant, avoiding denaturation
  • a freezer 18 °C
  • lecithin-based microemulsions will be prepared according to the
  • pH value for the activities of the above enzymes which are 7 8 and 8 8 respectively.
  • lecithin-based microemulsion for the preparation of the final product, which, if stored at a temperature of about 2-6 °C, retains its depilative action for at least three days
  • capsules will be stored at a temperature of about 2-6 °C Before using the preparations and before opening the vial, by slightly pressing the cap, the capsule will be broken and
  • the contained enzymic solution will be mixed with the microemulsion contained in the vial
  • the final microemulsion will be prepared containing the enzyme ⁇ -chymotrypsin or the enzyme trypsin at a final concentration of 0 05 mg/ml,
  • the enzymic solution may be kept in a separate vial and mixed in a similar way with the microemulsion solution just before use

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Dermatology (AREA)
  • Birds (AREA)
  • Epidemiology (AREA)
  • Cosmetics (AREA)
  • Medicinal Preparation (AREA)
  • Emulsifying, Dispersing, Foam-Producing Or Wetting Agents (AREA)
  • General Preparation And Processing Of Foods (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

The invention refers to depilatory preparations containing: proteolytic enzymes solubilized in microemulsions, formed with lecithin, aliphatic hydrocarbon, alipathic alcohol and buffer solution pH 7 to 9, value corresponding to the pH range near the optimum pH value for the catalytic activity of the proteolytic enzymes, to be applied for permanent enzymic depilation. The application of these preparations will assure, as shown by our experimental studies, more permanent depilation than the one resulting from other depilatory methods. The application of these preparations is suitable for every type of skin (fatty-resistant or dry-sensitive). The present invention introduces the use of microemulsions as a medium for the facilitated penetration of the enzymic activity in the epithelial cells of the skin, as shown by our studies.

Description

Lecithin-based microemulsions containing proteolytic enzymes and method for permanent enzymic depilation
The present invention refers to the depilatory preparations of lecithin-based microemulsions containing proteolytic enzymes The proposed invention introduces for
the first time the use of microemulsions as media for the soubilization of proteolytic enzymic systems, that assist the penetration of active molecules through the
5 phosphohpidic membranes In addition, the present invention refers to a depilation method, that applies the preparations of microemulsion containing the enzyme α- chymotrypsin, or the enzyme trypsin, for the depilation of any type of skin, as well as to
the depilation method based on the sequential application of the preparation of
microemulsion containing the enzyme α-chymotrypsin, followed by the application of the
10 preparation of microemulsion containing the enzyme trypsin, depending on the
particularities of each type of skin The application of the depilatory preparations of
microemulsions containing either the enzyme α-chymotrypsin, or the enzyme trypsin, or
the sequential application first of the preparation of microemulsion containing the enzyme α-chymotrypsin, followed by the application of the preparation of microemulsion
15 containing the enzyme trypsin, is carried out by simple spreading at appropriate intervals
Experimental studies published by us for the first time in the international literature, have shown that the action of the enzyme α-chymotrypsin provokes destruction
of the stem cells and of the hair follicle, explaining the positive results in depilation
Similar positive results in hair depilation also has trypsin, but with a milder action
20 Microemulsions are systems consisting of a continuous inert organic medium, where water droplets are dispersed with the help of lecithin acting as a natural surfactant
The dispersion of the aqueous phase, where the enzyme molecules α-chymotrypsin or trypsin are located, is achieved by simple and gentle shaking
Our invention improves the depilatory action of the enzymes α-chymotrypsin and trypsin, because of the fact that they are applied through the microemulsion The shape
of the microemulsion is such that allows its easy spreading over the whole surface of the
skin to be depilated Furthermore, the presence of the microemulsion offers the facility to be applied on every type of skin, such as the sensitive-dry ones, or the resistant-fatty ones
We have also observed that the sequential application first of the preparation of
microemulsion containing the enzyme α-chymotrypsin, followed by the application of the preparation of microemulsion containing the enzyme trypsin, provokes important degradation of the hair follicle, and that the sequential application first of the preparation
of microemulsion containing the enzyme α-chymotrypsin and then of the preparation of
microemulsion containing the enzyme trypsin has better depilatory results than the
sequential application first of the preparation of microemulsion containing the enzyme
trypsin followed by the application of the one containing the enzyme α-chymotrypsin We
have also observed that the sequential application first of the preparation of microemulsion containing α-chymotrypsin followed by the application of the preparation of microemulsion containing trypsin, has much better depilatory results than the application
of only one of each depilatory preparation of microemulsion containing either enzyme In
addition, we have observed that the sequential application of the preparation of microemulsion containing α-chymotrypsin followed by the application of the preparation of microemulsion containing trypsin, even at low concentrations of 0 05 mg/ml, had a
degrading effect on the hair follicle, rendering thus, the preparations with low enzyme
concentrations appropriate to be applied on dry, sensitive areas of the skin On the other hand we have observed that the use of high enzyme concentrations on dry-sensitive skins,
leads to a significant depilation but with an intense irritation Therefore it is recommended to apply on such type of skins preparations with low enzyme concentrations Moreover, the use of microemulsions as a carrier for the transdermal transfer of active
macromolecules, such as the proposed enzymes, offers a considerable improvement of the
enzyme depilation methodology, since the lecithin of the microemulsion interferes with the phospholipidic membrane of the epithelial cells, allowing the penetration of the enzymes in the cells
The proposed proteolytic enzymes α-chymotrypsin and trypsin will be used in
absolutely pure state. The enzymes will be stored lyophilized in a freezer (-18 °C) so that they are kept constant, avoiding denaturation For the production of the preparations the
enzymes will be dissolved in a solution of hydrochloric acid 0 001 M in doubly distilled
water (pH 3) obtaining concentrations of 1 to 5 mg/ml, and will be kept in a sterile
environment in a freezer (-18 °C) By this procedure the enzyme activity is kept unvaried for at least 8 months
In parallel, lecithin-based microemulsions will be prepared according to the
following For every ml of final solution, 0 75 to 1 ml of aliphatic hydrocarbon are mixed
with 20 to 75 mg of lecithin, 0 to 0.25 ml of aliphatic alcohol and 0 005 to 0 05 ml of buffer solution, pH 7 to 9 By mixing the above components, after gentle shaking, a clear solution (microemulsion) is obtained This solution is thermodynamically stable and has,
thus, unlimited time life To avoid perturbing the stability by a possible partial evaporation of the organic constituents and consequently altering the total microemulsion composition,
the solution is stored in cupped vessels
The choice of the buffer solutions used for the preparations of microemulsions containing the proteolytic enzymes α-chymotrypsin or trypsin, is based on the optimum
pH value for the activities of the above enzymes, which are 7 8 and 8 8 respectively One
possible buffer solution to use for the preparation of the lecithin-based microemulsions,
is the solution of 0 1 M tris-hydroxymethylamino-methane- hydrochloric acid, for the pH range 7.5-9, although many other buffers, covering the desired range of pH values, can be used
Just before applying the preparations the enzyme solution will be mixed with the
lecithin-based microemulsion for the preparation of the final product, which, if stored at a temperature of about 2-6 °C, retains its depilative action for at least three days
According to a suggested materialization of the present invention, in sterilized vials
of 2.5 ml will be placed 2 ml of lecithin-based microemulsion in isooctane, prepared by mixing 1 8 ml isooctane, 64 mg soy bean lecithin, 0 2 ml propanol- 1 and, either 0 03 ml buffer solution 0 1 M tris-hydroxymethylamino-methane-hydrochloric acid, pH 7 5-8 for
the preparations of α-chymotrypsin, or 0 03 ml buffer solution 0 1 M tris-hydroxymethyl-
aminomethane-hydrochloric acid, pH 8 5-9 for the preparations of trypsin In small
sterilized capsules located in the vials and incorporated in a special cap, will be placed 0.03 ml of the solution containing, either the enzyme α-chymotrypsin, or the enzyme
trypsin at a concentration of 3 4 mg/ml The microemulsion containing vials, and the small
capsules will be stored at a temperature of about 2-6 °C Before using the preparations and before opening the vial, by slightly pressing the cap, the capsule will be broken and
the contained enzymic solution will be mixed with the microemulsion contained in the vial
By repeatedly shaking the vial, the final microemulsion will be prepared containing the enzyme α-chymotrypsin or the enzyme trypsin at a final concentration of 0 05 mg/ml,
ready to be used Alternatively to the small capsule, the enzymic solution may be kept in a separate vial and mixed in a similar way with the microemulsion solution just before use
The application of the final preparations on the skin to be depilated will be carried out immediately after and for three consecutive days During this interval the preparations will
be stored in a freezer (-18°C)
By this way we finally offer for use
a) Preparations of lecithin-based microemulsions containing the enzyme α- chymotrypsin at a concentration of 0 05 mg/ml and pH 7.5-8, and according to a proposed application of the invention at pH 7 8
b) Preparations of lecithin-based microemulsions containing the enzyme trypsin
at a concentration of 0 05 mg/ml and pH 8 5-9, and according to a proposed application of the invention at pH 8 8
For areas of fatty resistant skins it is proposed to apply sequentially first the
preparation of microemulsion containing the enzyme α-chymotrypsin and then the
preparation of microemulsion containing the enzyme trypsin, whereas at areas of dry
sensitive skins it is proposed to apply the preparations containing trypsin It is up to the
judgement of the specialist to propose the use of the preparations of microemulsion containing either α-chymotrypsin or trypsin for sequential applications, to increase the
total quantity of either enzyme, depending on the type of skin

Claims

1 Depilatory preparation in liquid form, based on the action of proteolytic
enzymes solubilized in microemulsions and applied by simple spreading The concentration of the enzyme ranges from 0 01 to 0 1 mg/ml and the microemulsion consists of 40 to 150 mg lecithin, 1.5 to 2 ml aliphatic hydrocarbon, 0 to 0 5 ml aliphatic alcohol and 0 01 to
0 1 ml buffer solution, pH 7-9, covering the pH range that is optimum for the enzyme
activity
2 Depilatory preparation in liquid form, as mentioned in claim 1 , characterized by
the fact that the proteolytic enzyme is α-chymotrypsin, solubilized in microemulsions and
applied by simple spreading The concentration of the enzyme is 0 05 mg/ml and the
microemulsion is consists of 64 mg lecithin, 1 8 ml isooctane, 0.2 ml propanol-1 and 0 06 ml buffer solution of tris-hydroxymethylamino-rnethane-hydrochloric acid, pH 7 5-8,
covering the pH range that is optimum for the activity of the enzyme α-chymotrypsin
3 Depilatory preparation in liquid form, as mentioned in claim 1 , characterized by the fact that the proteolytic enzyme is trypsin, solubilized in microemulsions and applied by simple spreading The concentration of the enzyme is 0 05 mg/ml and the
microemulsion is consists of 64 mg lecithin, 1 8 ml isooctane, 0 2 ml propanol-1 and 0 06
ml buffer solution of tris-hydroxymethylamino-methane-hydrochloric acid, pH 7 5-8, covering the pH range that is optimum for the activity of the enzyme α-chymotrypsin
4 Method of preparation of depilatory preparations characterized by the
following 2 ml of a microemulsion containing 1 5 to 2 ml of aliphatic hydrocarbon, 40 to 150 mg of lecithin, 0 to 0 5 ml of aliphatic alcohol and 0 01 to 0 05 ml of buffer solution, pH 7 to 9, are placed in sterilized vials of 2 5 ml In small sterilized capsules located in the vials and incoφorated in the cap, 0.01 to 0.05 ml of a solution of proteolytic enzymes at
a concentration of about 1 to 5 mg/ml are placed Before using the preparations and
before opening the vial, by slightly pressing the cap the capsule is broken and the contained enzymic solution is mixed with the microemulsion contained in the vial. By repeatedly shaking the vial, the final depilatory preparation is obtained ready to be used
Alternatively to the small capsule, the enzymic solution can be kept in a separate vial and
mixed in a similar way with the microemulsion solution just before use
5 Method of depilation for intermediate particularities of skin types, characterized by the application of the depilatory preparation according to claim 2
6 Method of depilation for dry and sensitive types of skin, characterized by the application of the depilatory preparation according to claim 3
7 Method of depilation for fatty and resistant types of skin, characterized by the
sequential application first of the depilatory preparation according to claim 2, followed by
concecutive applications of the depilatory preparation according to claim 3
PCT/GR1997/000015 1996-05-22 1997-05-19 Lecithin-based microemulsions containing proteolytic enzymes and method for permanent enzymic depilation Ceased WO1997044005A1 (en)

Priority Applications (16)

Application Number Priority Date Filing Date Title
DE69719559T DE69719559T2 (en) 1996-05-22 1997-05-19 LECITHIN-BASED MICROEMULSIONS CONTAINING PROTEOLYTIC ENZYMS AND METHOD FOR PERMANENTLY ENZYMATIC HAIR REMOVAL
AU27098/97A AU733595B2 (en) 1996-05-22 1997-05-19 Lecithin-based microemulsions containing proteolytic enzymes and method for permanent enzymic depilation
BR9709341-6A BR9709341A (en) 1996-05-22 1997-05-19 Microemulsions based on lecithin containing proteolytic enzymes and method for permanent enzymatic hair removal
EP97920886A EP0955991B1 (en) 1996-05-22 1997-05-19 Lecithin-based microemulsions containing proteolytic enzymes and method for permanent enzymic depilation
PL97330602A PL187389B1 (en) 1996-05-22 1997-05-19 Lecithin-based microemulsion containing proteolytic enzymes and method of performing permanent enzymatic depilation
CA002255649A CA2255649C (en) 1996-05-22 1997-05-19 Lecithin-based microemulsions containing proteolytic enzymes and method for permanent enzymic depilation
AT97920886T ATE233537T1 (en) 1996-05-22 1997-05-19 LECITHIN-BASED MICROEMULSIONS CONTAINING PROTEOLYTIC ENZYMES AND METHOD FOR THE PERMANENT ENZYMATIC REMOVAL OF HAIR
APAP/P/1998/001405A AP1077A (en) 1996-05-22 1997-05-19 Lecithin-based microemulsion containing proteolytic enzymes and method for permanent enzymic depilation.
IL12715097A IL127150A (en) 1996-05-22 1997-05-19 Lecithin-based microemulsions containing proteolytic enzymes and method for permanent enzymic depilation
US09/180,904 US6203791B1 (en) 1996-05-22 1997-05-19 Lecithin-based microemulsions containing proteolytic enzymes and method for permanent enzymic depilation
DK97920886T DK0955991T3 (en) 1996-05-22 1997-05-19 Lecithin-based microemulsions containing proteolytic enzymes and methods for permanent enzymatic hair removal
NZ332898A NZ332898A (en) 1996-05-22 1997-05-19 depilatory preparation containing a proteolytic enzyme solubilised in a microemulsion containing lecithin, aliphatic hydrocarbon, aliphatic alcohol and a buffer solution
SK1592-98A SK281923B6 (en) 1996-05-22 1997-05-19 Lecithin-based microemulsions containing proteolytic enzymes and method for permanent enzymic depilation
BG102938A BG63658B1 (en) 1996-05-22 1998-11-19 Depilatory preparation, containing proteolytic enzymes and methods for depilation
IS4899A IS2106B (en) 1996-05-22 1998-11-20 Lecithin-based microemulsion containing protein-cleaving enzymes, and a method for permanent enzyme permanent removal
NO985439A NO985439D0 (en) 1996-05-22 1998-11-23 Lecithin-based microemulsions containing proteolytic enzymes and a method for permanent hair removal with enzymes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GR960100168 1996-05-22
GR96100168 1996-05-22

Publications (1)

Publication Number Publication Date
WO1997044005A1 true WO1997044005A1 (en) 1997-11-27

Family

ID=10942400

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GR1997/000015 Ceased WO1997044005A1 (en) 1996-05-22 1997-05-19 Lecithin-based microemulsions containing proteolytic enzymes and method for permanent enzymic depilation

Country Status (23)

Country Link
US (1) US6203791B1 (en)
EP (1) EP0955991B1 (en)
AP (1) AP1077A (en)
AT (1) ATE233537T1 (en)
AU (1) AU733595B2 (en)
BG (1) BG63658B1 (en)
BR (1) BR9709341A (en)
CA (1) CA2255649C (en)
CZ (1) CZ293874B6 (en)
DE (1) DE69719559T2 (en)
DK (1) DK0955991T3 (en)
ES (1) ES2190530T3 (en)
GR (1) GR1002706B (en)
HU (1) HUP9904247A3 (en)
IL (1) IL127150A (en)
IS (1) IS2106B (en)
NO (1) NO985439D0 (en)
NZ (1) NZ332898A (en)
PL (1) PL187389B1 (en)
PT (1) PT955991E (en)
RU (1) RU2183452C2 (en)
SK (1) SK281923B6 (en)
WO (1) WO1997044005A1 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2777180A1 (en) * 1998-04-10 1999-10-15 Lvmh Rech COSMETIC OR DERMATOLOGICAL COMPOSITIONS IN THE FORM OF WATER-IN-OIL TYPE EMULSIONS CONTAINING LIGHT HYDROCARBONS AND PHOSPHOLIPIDS AND THEIR PREPARATION METHOD
WO2005034892A1 (en) 2003-10-10 2005-04-21 Cosmedical Aps Hair growth inhibition
WO2007042824A1 (en) * 2005-10-14 2007-04-19 Reckitt Benckiser (Uk) Limited Microemulsion and shaving apparatus comprising a microemulsion

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6375948B1 (en) 1999-07-12 2002-04-23 Kao Corporation Treating method for suppressing hair growth
WO2004103539A2 (en) * 2003-05-16 2004-12-02 Velocys Inc. Process for forming an emulsion using microchannel process technology
US7485671B2 (en) * 2003-05-16 2009-02-03 Velocys, Inc. Process for forming an emulsion using microchannel process technology
EP1804964A1 (en) 2004-10-01 2007-07-11 Velocys Inc. Multiphase mixing process using microchannel process technology
CA2587412C (en) * 2004-11-17 2013-03-26 Velocys Inc. Emulsion process using microchannel process technology
EP2236617A1 (en) * 2009-03-31 2010-10-06 Leukocare Ag Methods of terminal sterilization of biofunctional compositions

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0622069A1 (en) * 1992-12-29 1994-11-02 Evangelia Protopapa Enzymic preparations containing papain or chymotrypsin and methods of permanent enzymic depilation

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1091591C (en) * 1994-02-04 2002-10-02 利珀克尔集团公司 Lipophilic carrier preparations
FI100692B (en) * 1994-05-24 1998-02-13 Leiras Oy Process for the preparation of pharmaceutical compositions based on gels of microemulsions and new microemulsion-based gels

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0622069A1 (en) * 1992-12-29 1994-11-02 Evangelia Protopapa Enzymic preparations containing papain or chymotrypsin and methods of permanent enzymic depilation

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2777180A1 (en) * 1998-04-10 1999-10-15 Lvmh Rech COSMETIC OR DERMATOLOGICAL COMPOSITIONS IN THE FORM OF WATER-IN-OIL TYPE EMULSIONS CONTAINING LIGHT HYDROCARBONS AND PHOSPHOLIPIDS AND THEIR PREPARATION METHOD
WO2005034892A1 (en) 2003-10-10 2005-04-21 Cosmedical Aps Hair growth inhibition
WO2007042824A1 (en) * 2005-10-14 2007-04-19 Reckitt Benckiser (Uk) Limited Microemulsion and shaving apparatus comprising a microemulsion
JP2009511561A (en) * 2005-10-14 2009-03-19 レキット ベンキサー （ユーケイ） リミテッド Microemulsion and shaving device containing microemulsion

Also Published As

Publication number Publication date
EP0955991B1 (en) 2003-03-05
BG102938A (en) 1999-07-30
IS2106B (en) 2006-05-15
US6203791B1 (en) 2001-03-20
DE69719559T2 (en) 2004-02-26
SK281923B6 (en) 2001-09-11
PT955991E (en) 2003-07-31
CZ372398A3 (en) 1999-02-17
HUP9904247A3 (en) 2003-06-30
IL127150A (en) 2001-08-08
IS4899A (en) 1998-11-20
AU733595B2 (en) 2001-05-17
CA2255649A1 (en) 1997-11-27
NO985439L (en) 1998-11-23
CZ293874B6 (en) 2004-08-18
AU2709897A (en) 1997-12-09
ES2190530T3 (en) 2003-08-01
IL127150A0 (en) 1999-09-22
RU2183452C2 (en) 2002-06-20
NO985439D0 (en) 1998-11-23
DE69719559D1 (en) 2003-04-10
BR9709341A (en) 2000-01-04
GR1002706B (en) 1997-05-26
HUP9904247A1 (en) 2000-04-28
PL330602A1 (en) 1999-05-24
EP0955991A1 (en) 1999-11-17
DK0955991T3 (en) 2003-06-02
ATE233537T1 (en) 2003-03-15
PL187389B1 (en) 2004-06-30
AP1077A (en) 2002-07-01
BG63658B1 (en) 2002-08-30
CA2255649C (en) 2002-04-09
AP9801405A0 (en) 1998-12-31
NZ332898A (en) 2000-06-23
SK159298A3 (en) 1999-07-12

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