WO1997049832A3 - Method of identifying cytochrome p450 - Google Patents

Method of identifying cytochrome p450 Download PDF

Info

Publication number
WO1997049832A3
WO1997049832A3 PCT/CA1997/000488 CA9700488W WO9749832A3 WO 1997049832 A3 WO1997049832 A3 WO 1997049832A3 CA 9700488 W CA9700488 W CA 9700488W WO 9749832 A3 WO9749832 A3 WO 9749832A3
Authority
WO
WIPO (PCT)
Prior art keywords
cytochrome
mrna
cells
nucleic acid
agent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/CA1997/000488
Other languages
French (fr)
Other versions
WO1997049832A2 (en
Inventor
P Martin Petkovich
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Queens University at Kingston
Original Assignee
Queens University at Kingston
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/724,466 external-priority patent/US6063606A/en
Application filed by Queens University at Kingston filed Critical Queens University at Kingston
Priority to AU33321/97A priority Critical patent/AU3332197A/en
Priority to EP97929069A priority patent/EP0935676A2/en
Priority to JP10502013A priority patent/JP2000513928A/en
Publication of WO1997049832A2 publication Critical patent/WO1997049832A2/en
Publication of WO1997049832A3 publication Critical patent/WO1997049832A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)

Abstract

A method for identifying a nucleotide sequence encoding a cytochrome P450, the cytochrome being inducible or suppressible in cells of a given type by an agent. The method includes: exposing a first group of the cells to the agent so as to induce or suppress expression of the cytochrome P450; isolating first mRNA from the cells; isolating second mRNA from a second group of the cells which have not been exposed to the agent so as to induce or suppress expression of the cytochrome P450; amplifying the first and second mRNA, respectively, in the presence of an oligo(dT) based first nucleic acid primer sufficient to prime synthesis from a poly(A) tail and a second nucleic acid primer substantially complementary to a nucleic acid sequence encoding a conserved region of a known cytochrome P450; displaying amplified products of the first mRNA and amplified products of the second mRNA to detect differences therebetween; and identifying said nucleotide sequence encoding said inducible or suppressible cytochrome P450.
PCT/CA1997/000488 1996-06-21 1997-06-23 Method of identifying cytochrome p450 Ceased WO1997049832A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU33321/97A AU3332197A (en) 1996-06-21 1997-06-23 Method of identifying cytochrome p450
EP97929069A EP0935676A2 (en) 1996-06-21 1997-06-23 Method of identifying cytochrome p450
JP10502013A JP2000513928A (en) 1996-06-21 1997-06-23 Method for identifying cytochrome P450

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US66754696A 1996-06-21 1996-06-21
US08/667,546 1996-06-21
US08/724,466 US6063606A (en) 1996-06-21 1996-10-01 Retinoid metabolizing protein
US08/724,466 1996-10-01

Publications (2)

Publication Number Publication Date
WO1997049832A2 WO1997049832A2 (en) 1997-12-31
WO1997049832A3 true WO1997049832A3 (en) 1998-03-26

Family

ID=27099706

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/CA1997/000440 Ceased WO1997049815A1 (en) 1996-06-21 1997-06-23 Retinoid metabolizing protein
PCT/CA1997/000488 Ceased WO1997049832A2 (en) 1996-06-21 1997-06-23 Method of identifying cytochrome p450

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/CA1997/000440 Ceased WO1997049815A1 (en) 1996-06-21 1997-06-23 Retinoid metabolizing protein

Country Status (9)

Country Link
US (1) US6306624B1 (en)
EP (2) EP0935676A2 (en)
JP (2) JP2000513928A (en)
AT (1) ATE309370T1 (en)
AU (2) AU3332197A (en)
CA (2) CA2257352C (en)
DE (1) DE69734586T2 (en)
IN (1) IN186424B (en)
WO (2) WO1997049815A1 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1196545B1 (en) * 1999-07-27 2006-10-11 Basf Aktiengesellschaft Electron donor system for enzymes and its use for the biochemical conversion of substrates
EP1296643A4 (en) * 1999-09-17 2005-07-27 Isis Pharmaceuticals Inc ANTISENSE MODULATION OF BETA-TGF EXPRESSION
US7537919B2 (en) 1999-12-16 2009-05-26 Cytochroma Inc. Cytochrome P450RAI-2 and related proteins
WO2002048334A2 (en) * 2000-12-15 2002-06-20 Cytochroma Inc. P450rai-2 (p450 cytochrome 26b), encoding nucleic acid molecules and methods and uses thereof
US7115558B2 (en) * 1999-12-16 2006-10-03 Cytochroma Inc. P450RAI-2(P450 Cytochrome 26B), encoding nucleic acid molecules and methods and uses thereof
US6444222B1 (en) * 2001-05-08 2002-09-03 Verigen Transplantation Services International Ag Reinforced matrices
CA2448933C (en) 2001-05-23 2012-05-15 Cytochroma Inc. A retinoic acid metabolizing cytochrome p450
JP2005512532A (en) * 2001-08-03 2005-05-12 ディヴァーサ コーポレイション P450 enzymes, nucleic acids encoding them and their production and use
US7288407B2 (en) 2002-05-02 2007-10-30 Cytochroma, Inc. Stable cytochrome P450 24 (CYP24) expressing cell line and methods and uses thereof
US7427670B2 (en) 2003-12-19 2008-09-23 Cytochroma Inc. Cytochrome P450 24 (CYP24) monoclonal antibody and methods and uses thereof
EP2524038A4 (en) * 2010-01-12 2013-11-20 Isis Pharmaceuticals Inc MODULATION OF TRANSFORMANT GROWTH FACTOR EXPRESSION BETA 1

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5429948A (en) * 1988-03-02 1995-07-04 Gentest Corporation Human cell line stably expressing 5cDNAS encoding procarcinogen-activating enzymes and related mutagenicity assays

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2100582A1 (en) * 1991-03-18 1992-09-19 David J. Mangelsdorf Response element compositions and assays employing same
EP0640093A4 (en) * 1992-04-28 1996-06-05 Univ Michigan CRABP-I AND CRABP-II HUMAN.
EP0721984A1 (en) * 1995-01-13 1996-07-17 Leuven Research & Development V.Z.W. Enhancer element of gene expression
US5650279A (en) * 1995-01-27 1997-07-22 Allergan, Inc. Gene sequence induced in skin by retinoids

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5429948A (en) * 1988-03-02 1995-07-04 Gentest Corporation Human cell line stably expressing 5cDNAS encoding procarcinogen-activating enzymes and related mutagenicity assays

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
COON ET AL.: "CYTOCHROME P450: PROGRESS AND PREDICTIONS", FASEB J., vol. 6, 1992, pages 669 - 673, XP002046659 *
DUNKOV ET AL.: "CYTOCHROME P450 GENE CLUSTERS IN DROSOPHILA MELANOGASTER", MOL.GEN.GENET., vol. 251, 12 June 1996 (1996-06-12), pages 290 - 297, XP002046658 *
LIANG P ET AL: "DIFFERENTIAL DISPLAY OF EUKARYOTIC MESSENGER RNA BY MEANS OF THE POLYMERASE CHAIN REACTION", SCIENCE, vol. 257, no. 5072, 14 August 1992 (1992-08-14), pages 967 - 971, XP000508268 *
SUZUKI ET AL.: "INDUCTION OF A NOVEL CYTOCHROME P450 (CYP93 FAMILY) BY METHYL JASMONATE IN SOYBEAN SUSPENSION-CULTURED CELLS", FEBS LETTERS, vol. 383, 1996, pages 83 - 86, XP002046657 *

Also Published As

Publication number Publication date
JP4283342B2 (en) 2009-06-24
DE69734586T2 (en) 2006-08-10
AU3332197A (en) 1998-01-14
WO1997049815A1 (en) 1997-12-31
WO1997049832A2 (en) 1997-12-31
EP0910644A1 (en) 1999-04-28
CA2257352C (en) 2011-11-29
AU3162097A (en) 1998-01-14
JP2000513927A (en) 2000-10-24
ATE309370T1 (en) 2005-11-15
DE69734586D1 (en) 2005-12-15
CA2257352A1 (en) 1997-12-31
JP2000513928A (en) 2000-10-24
EP0935676A2 (en) 1999-08-18
CA2257180A1 (en) 1997-12-31
US6306624B1 (en) 2001-10-23
EP0910644B1 (en) 2005-11-09
IN186424B (en) 2001-09-01

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