WO1998013527A3 - Compositions and methods for enhancing hybridization specificity - Google Patents

Compositions and methods for enhancing hybridization specificity Download PDF

Info

Publication number
WO1998013527A3
WO1998013527A3 PCT/US1997/017413 US9717413W WO9813527A3 WO 1998013527 A3 WO1998013527 A3 WO 1998013527A3 US 9717413 W US9717413 W US 9717413W WO 9813527 A3 WO9813527 A3 WO 9813527A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
compositions
specificity
nucleic acid
organic solvents
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1997/017413
Other languages
French (fr)
Other versions
WO1998013527A2 (en
Inventor
Ness Jeffrey Van
John Tabone
Lori K Garrison
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
UCB Manufacturing Inc
Original Assignee
Darwin Molecular Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Darwin Molecular Corp filed Critical Darwin Molecular Corp
Priority to JP51598298A priority Critical patent/JP2002514909A/en
Priority to CA002266847A priority patent/CA2266847A1/en
Priority to EP99107983A priority patent/EP0952228A3/en
Priority to AU45997/97A priority patent/AU4599797A/en
Priority to EP97944521A priority patent/EP0958378A2/en
Publication of WO1998013527A2 publication Critical patent/WO1998013527A2/en
Publication of WO1998013527A3 publication Critical patent/WO1998013527A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)

Abstract

Compositions and methods are provided for increasing the specificity of a probe nucleic acid for a target nucleic acid in a hybridization solution. An abasic residue, deoxyNebularine residue, or a hybotrope is used to increase specificity. A method is provided for identifying useful hybotropes, including salts, water miscible organic solvents, aprotic solvents and organic solvents, on the basis of enthalpy considerations. Hybotropic hybridization and modified oligonucleotides may be used in amplification reactions, such as PCR, sequence analysis methods, and genomic screening methods.
PCT/US1997/017413 1996-09-24 1997-09-24 Compositions and methods for enhancing hybridization specificity Ceased WO1998013527A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP51598298A JP2002514909A (en) 1996-09-24 1997-09-24 Compositions and methods for enhancing hybridization specificity
CA002266847A CA2266847A1 (en) 1996-09-24 1997-09-24 Compositions and methods for enhancing hybridization specificity
EP99107983A EP0952228A3 (en) 1996-09-24 1997-09-24 Compositions and methods for enhancing hybridization specificity
AU45997/97A AU4599797A (en) 1996-09-24 1997-09-24 Compositions and methods for enhancing hybridization specificity
EP97944521A EP0958378A2 (en) 1996-09-24 1997-09-24 Compositions and methods for enhancing hybridization specificity

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US2662196P 1996-09-24 1996-09-24
US71913296A 1996-09-24 1996-09-24
US08/719,132 1996-09-24
US60/026,621 1996-09-24

Publications (2)

Publication Number Publication Date
WO1998013527A2 WO1998013527A2 (en) 1998-04-02
WO1998013527A3 true WO1998013527A3 (en) 1998-08-20

Family

ID=26701464

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1997/017413 Ceased WO1998013527A2 (en) 1996-09-24 1997-09-24 Compositions and methods for enhancing hybridization specificity

Country Status (5)

Country Link
EP (1) EP0958378A2 (en)
JP (1) JP2002514909A (en)
AU (1) AU4599797A (en)
CA (1) CA2266847A1 (en)
WO (1) WO1998013527A2 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6872521B1 (en) * 1998-06-16 2005-03-29 Beckman Coulter, Inc. Polymerase signaling assay
WO2001023618A2 (en) * 1999-09-30 2001-04-05 Qiagen Genomics, Inc. Compositions and methods for reducing oligonucleotide hybridization and priming specificity
WO2002018659A2 (en) * 2000-08-30 2002-03-07 Haplogen, Llc Method for determining alleles
DK1370690T3 (en) 2001-03-16 2012-07-09 Kalim Mir Arrays and methods for using them
US6753145B2 (en) 2001-07-05 2004-06-22 Agilent Technologies, Inc. Buffer composition and method for hybridization of microarrays on adsorbed polymer siliceous surfaces
CA2524572C (en) * 2003-05-01 2018-07-10 Gen-Probe Incorporated Oligonucleotides comprising a molecular switch having enhanced sensitivity to the presence of a mismatch
CA2494571C (en) * 2003-12-02 2010-02-09 F.Hoffmann-La Roche Ag Oligonucleotides containing molecular rods
KR100601986B1 (en) * 2005-02-02 2006-07-18 삼성전자주식회사 How to hybridize genes
KR101110013B1 (en) * 2007-10-05 2012-02-29 (주)바이오니아 Primers for pcr amplification comprising abasic parts within the primer sequences
WO2017041005A1 (en) 2015-09-03 2017-03-09 Abbott Molecular Inc. Hybridization buffers comprising an alkyl diester
US10457981B2 (en) 2016-01-08 2019-10-29 Abbott Molecular Inc. Hybridization buffers
KR20230043819A (en) * 2020-06-03 2023-03-31 큐랄리스 코포레이션 Treatment of neurological diseases using modulators of gene transcripts
KR102750775B1 (en) * 2021-09-13 2025-01-09 건국대학교 산학협력단 Method of salt mediated immobilization of nucleic acids, the solid support including nucleic acids immobilized thereby the method, lateral flow assay strips including the solid support and method of detection of target nucleic acid

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987006621A1 (en) * 1986-05-02 1987-11-05 David Gillespie Chaotropic method for evaluating nucleic acids in a biological sample
WO1990012116A1 (en) * 1989-04-05 1990-10-18 Gene-Trak Systems Hybridization promotion reagents
EP0420260A2 (en) * 1989-09-29 1991-04-03 F. HOFFMANN-LA ROCHE & CO. Aktiengesellschaft Biotin-labelled DNA by polymerase chain reaction and detection thereof
WO1992015708A1 (en) * 1991-02-27 1992-09-17 Amoco Corporation Methods for improving the sensitivity of hybridization assays
WO1992018649A1 (en) * 1991-04-12 1992-10-29 Microprobe Corporation Compositions and methods for improved extraction and hybridization of nucleic acid
WO1993020234A1 (en) * 1992-03-31 1993-10-14 E.I. Du Pont De Nemours And Company A rapid, high capacity nucleic acid based assay
WO1994006815A1 (en) * 1992-09-11 1994-03-31 Isis Pharmaceuticals, Inc. Oligonucleotide and nucleotide amine analogs, methods of synthesis and use
EP0628571A1 (en) * 1992-09-07 1994-12-14 Nippon Steel Corporation Novel peptide, and antithrombotic agent, anticoagulant for extracorporeal circulation, cell fusion inhibitor, cancer metastasis inhibitor, protective for platelet preparation for transfusion and pack of platelet preparation for transfusion
WO1995019776A1 (en) * 1994-01-19 1995-07-27 The Trustees Of Columiba University In The City Of New York A method of treating glaucoma
WO1995030774A1 (en) * 1994-05-05 1995-11-16 Beckman Instruments, Inc. Oligonucleotide repeat arrays

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987006621A1 (en) * 1986-05-02 1987-11-05 David Gillespie Chaotropic method for evaluating nucleic acids in a biological sample
WO1990012116A1 (en) * 1989-04-05 1990-10-18 Gene-Trak Systems Hybridization promotion reagents
EP0420260A2 (en) * 1989-09-29 1991-04-03 F. HOFFMANN-LA ROCHE & CO. Aktiengesellschaft Biotin-labelled DNA by polymerase chain reaction and detection thereof
WO1992015708A1 (en) * 1991-02-27 1992-09-17 Amoco Corporation Methods for improving the sensitivity of hybridization assays
WO1992018649A1 (en) * 1991-04-12 1992-10-29 Microprobe Corporation Compositions and methods for improved extraction and hybridization of nucleic acid
WO1993020234A1 (en) * 1992-03-31 1993-10-14 E.I. Du Pont De Nemours And Company A rapid, high capacity nucleic acid based assay
EP0628571A1 (en) * 1992-09-07 1994-12-14 Nippon Steel Corporation Novel peptide, and antithrombotic agent, anticoagulant for extracorporeal circulation, cell fusion inhibitor, cancer metastasis inhibitor, protective for platelet preparation for transfusion and pack of platelet preparation for transfusion
WO1994006815A1 (en) * 1992-09-11 1994-03-31 Isis Pharmaceuticals, Inc. Oligonucleotide and nucleotide amine analogs, methods of synthesis and use
WO1995019776A1 (en) * 1994-01-19 1995-07-27 The Trustees Of Columiba University In The City Of New York A method of treating glaucoma
WO1995030774A1 (en) * 1994-05-05 1995-11-16 Beckman Instruments, Inc. Oligonucleotide repeat arrays

Also Published As

Publication number Publication date
EP0958378A2 (en) 1999-11-24
WO1998013527A2 (en) 1998-04-02
CA2266847A1 (en) 1998-04-02
AU4599797A (en) 1998-04-17
JP2002514909A (en) 2002-05-21

Similar Documents

Publication Publication Date Title
US5972618A (en) Detection of mutations in nucleic acids by chemical cleavage
EP2705165B1 (en) Quantitative nuclease protection assay (qnpa) and sequencing (qnps) improvements
JP5368108B2 (en) Sequencing and genotyping methods using reversible stop nucleotides
WO2001088089A3 (en) Single copy genomic hybridization probes and method of generating same
US8431347B2 (en) Isothermal strand displacement amplification using primers containing a non-regular base
WO2001013086A3 (en) Detection of nucleic acids
EP1256632A3 (en) High throughput polymorphism screening
NZ506333A (en) An assay for detecting and quantifying the presence of particular nucleic acid (DNA or RNA) sequences
EP1856293A2 (en) Methods and compositions for assay readouts on multiple analytical platforms
EA200100402A1 (en) METHODS OF AMPLIFICATION AND SEQUENCYING OF NUCLEIC ACIDS
BR9407712A (en) Methods and compositions for effective nucleic acid sequencing
WO2002057491A3 (en) Direct multiplex characterization of genomic dna
WO1999014375A3 (en) Dna typing by mass spectrometry with polymorphic dna repeat markers
CA2183758A1 (en) Human papilloma virus detection in a nucleic acid amplification process using general primers
ATE403747T1 (en) METHOD FOR AMPLIFICATION OF SIGNAL-FLANKING SEQUENCES FROM UNKNOWN GENOMIC DATA
CA2261576A1 (en) Probes for detecting and identifying helicobacter pylori
WO2000022171A3 (en) Systems and methods for sequencing by hybridization
CN111074353A (en) Whole-genome methylation library single-strand library construction method and the resulting whole-genome methylation library
US20240218439A1 (en) Multiplexed unbiased nucleic acid amplification method
CA2142156A1 (en) Nucleic Acid Sequences Specific for Mycobacterium Kansasii
EP0952228A3 (en) Compositions and methods for enhancing hybridization specificity
CN101153338A (en) DNA Sequencing Method Based on Base Modification Protected Reciprocating Extension
Milla et al. Use of the Restriction Enzyme Ava I and Exo− Bst Polymerase in Strand Displacement Amplification
WO1999053095A3 (en) Biallelic markers
EP1207209A3 (en) Methods using arrays for detection of single nucleotide polymorphisms

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AL AM AT AU BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GE GH HU ID IL IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG UZ VN YU ZW AM AZ BY KG KZ MD RU TJ TM

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH KE LS MW SD SZ UG ZW AT BE CH DE DK ES FI FR GB GR IE IT LU MC NL PT

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
AK Designated states

Kind code of ref document: A3

Designated state(s): AL AM AT AU BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GE GH HU ID IL IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG UZ VN YU ZW AM AZ BY KG KZ MD RU TJ TM

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH KE LS MW SD SZ UG ZW AT BE CH DE DK ES FI FR GB GR IE IT LU MC NL PT

ENP Entry into the national phase

Ref document number: 2266847

Country of ref document: CA

Ref country code: CA

Ref document number: 2266847

Kind code of ref document: A

Format of ref document f/p: F

WWE Wipo information: entry into national phase

Ref document number: 1997944521

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWP Wipo information: published in national office

Ref document number: 1997944521

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 1997944521

Country of ref document: EP