WO1998024888A2 - Polypeptides encoded by a human lipase-like gene, compositions and methods - Google Patents

Abstract

Lipoprotein Lipase like polypeptides, nucleic acids encoding said polypeptides, antisense sequences, and antibodies to said polypeptides are disclosed. Also disclosed are methods for the preparation of said polypeptides in a recombinant system and for the use of said polypeptides to screen for agonists and or antagonists of said polypeptides. Also disclosed are methods and compositions for the treatment of disorders of lipid metabolism.

Description

LLG POLYPEPTIDES OF THE TRIACYLGLYCEROL LIPASE FAMILY, AND COMPOSITIONS AND METHODS FOR THEIR USE IN ENZYMATIC HYDROLYSIS, AND PROTEIN AND GENE THERAPIES

This application claims the benefit of two copending provisional applications, 60/032,254 and 60/032,783, both of which were filed December 6, 1996.

FIELD OF THE INVENTION

This invention relates to polypeptides of the triacylglycerol lipase family, to nucleic acids encoding said polypeptides, to antisense sequences derived from said nucleic acids, and to antibodies against said polypeptides. This invention also relates to the preparation of said polypeptides using recombinant technology, and to the use of said polypeptides to screen for agonists and or antagonists of said polypeptides. This invention also relates to methods for the therapeutic use of such polypeptides, and of the nucleic acid sequences encoding the same in pharmaceutical, including gene therapeutic, compositions for the treatment of disorders of lipid and lipoprotein metabolism.

BACKGROUND OF THE INVENTION

A) Lipids

Lipids are water-insoluble organic biomolecules, which are essential components of diverse biological functions, including the storage, transport, and metabolism of energy, and membrane structure and fluidity. Lipids are derived from two sources in man and other animals: some lipids are ingested as dietary fats and oils and other lipids are biosynthesized by the human or animal. In mammals at least 10% of the body weight is lipid, the bulk of which is in the form of triacylglycerols.

Triacylglycerols, also known as triglycerides and triacylglycerides, are made up of three fatty acids esterified to glycerol. Dietary triacylglycerols are stored in adipose tissues as a source of energy, or hydrolyzed in the digestive tract by triacylglycerol lipases, the most important of which is pancreatic lipase. Triacylglycerols are transported between tissues in the form of lipoproteins. Lipoproteins are micelle-like assemblies found in plasma which contain varying proportions of different types of lipids and proteins (called apoproteins). There are five main classes of plasma lipoproteins, the major function of which is lipid transport. These classes are, in order of increasing density, chylomicrons, very low density lipoproteins (VLDL), intermediate-density lipoproteins (IDL), low density lipoproteins (LDL), and high density lipoproteins (HDL). Although many types of lipid are found associated with each lipoprotein class, each class transports predominantly one type of lipid: triacylglycerols described above are transported in chylomicrons, VLDL, and IDL; while phospholipids and cholesterol esters are transported in HDL and LDL respectively. Phospholipids are di-fatty acid esters of glycerol phosphate, also containing a polar group coupled to the phosphate. Phospholipids are important structural components of cellular membranes. Phospholipids are hydrolyzed by enzymes called phospholipases. Phosphatidylcholine, an exemplary phospholipid, is a major component of most eukaryotic cell membranes. Cholesterol is the metabolic precursor of steroid hormones and bile acids as well as an essential constituent of cell membranes. In man and other animals, cholesterol is ingested in the diet and also synthesized by the liver and other tissues. Cholesterol is transported between tissues in the form of cholesteryl esters in LDLs and other lipoproteins.

Membranes surround every living cell, and serve as a barrier between the intracellular and extracellular compartments. Membranes also enclose the eukaryotic nucleus, make up the endoplasmic reticulum, and serve specialized functions such as in the myelin sheath that surrounds axons. A typical membrane contains about 40% lipid and 60% protein, but there is considerable variation. The major lipid components are phospholipids, specifically phosphatidylcholine and phosphatidylethanolamine, and cholesterol. The physicochemical properties of membranes, such as fluidity, can be changed by modification of either the fatty acid profiles of the phospholipids or the cholesterol content. Modulating the composition and organization of membrane lipids also modulates membrane-dependent cellular functions, such as receptor activity, endocytosis, and cholesterol flux.

B) Enzymes

The triacylglycerol lipases are a family of enzymes which play several pivotal roles in the metabolism of lipids in the body. Three members of the human triacylglycerol lipase family have been described: pancreatic lipase, lipoprotein lipase, and hepatic lipase (Goldberg, I.J., Le, N.-A., Ginsberg, H.N., Krauss, R.M., and Lindgren, F.T. (1988) J. Clin. Invest. 81 ,561-568; Goldberg, I.J., Le, N., Paterniti J.R., Ginsberg, H.N., Lindgren, F.T., and Brown, W.V. (1982) J. Clin. Invest. 70,1184-1 192; Hide, W.A., Chan, L., and Li, W.-H. (1992) J. Lipid. Res. 33,167- 178). Pancreatic lipase is primarily responsible for the hydrolysis of dietary lipids. Variants of pancreatic lipase have been described, but their physiological role has not been determined (Giller, T., Buchwald, P., Blum-Kaelin, D., and Hunziker, W. (1992) J. Biol. Chem. 267,16509- 16516). Lipoprotein lipase is the major enzyme responsible for the distribution and utilization of triglycerides in the body. Lipoprotein lipase hydrolyzes triglycerides in both chylomicrons and VLDL. Hepatic lipase hydrolyzes triglycerides in IDL and HDL, and is responsible for lipoprotein remodeling. Hepatic lipase also functions as a phospholipase, and hydrolyzes phospholipids in HDL.

Phospholipases play important roles in the catabolism and remodeling of the phospholipid component of lipoproteins and the phospholipids of membranes. Phospholipases also play a role in the release of arachidonic acid and the subsequent formation of prostaglandins, leukotrienes, and other lipids which are involved in a variety of inflammatory processes.

The lipase polypeptides encoded by these lipase genes are approximately 450 amino acids in length with leader signal peptides to facilitate secretion. The lipase proteins are comprised of two principal domains (Winkler, K., D'Arcy, A., and Hunziker, W. (1990) Nature 343, 771-774). The amino terminal domain contains the catalytic site while the carboxyl domain is believed to be responsible for substrate binding, cofactor association, and interaction with cell receptors (Wong, H., Davis, R.C., Nikazy, J., Seebart, K.E., and Schotz, M.C. (1991) Proc. Natl. Acad. Sci. USA 88,11290-1 1294; van Tilbeurgh, H., Roussel, A., Lalouel, J.-M., and Cambillau, C. (1994) J. Biol. Chem. 269,4626-4633; Wong, H., Davis, R.C., Thuren, T., Goers, J.W., Nikazy, J., Waite, M., and Schotz, M.C. (1994) J. Biol. Chem. 269,10319-10323; Chappell, D.A., Inoue, I., Fry, G.L., Pladet, M.W., Bowen, S.L., Iverius, P.-H., Lalouel, J.-M., and Strickland, D.K. (1994) J. Biol. Chem. 269,18001-18006). The overall level of amino acid homology between members of the family is 22-65%, with local regions of high homology corresponding to structural homologies which are linked to enzymatic function.

The naturally occurring lipoprotein lipase protein is glycosylated, and glycosylation is necessary for LPL enzymatic activity (Semenkovich, C.F., Luo, C.-C, Nakanishi, M.K., Chen, S.- H., Smith, L C, and Chan L. (1990) J. Biol. Chem. 265, 5429-5433). There are two sites for N- linked glycosylation in hepatic and lipoprotein lipase and one in pancreatic lipase. Additionally, four sets of cysteines form disulfide bridges which are essential in maintaining structural integrity for enzymatic activity (Lo, J.-Y., Smith, L.C., and Chan, L. (1995) Biochem. Biophys. Res. Commun. 206, 266-271 ; Brady, L., Brzozowski, A.M., Derewenda, Z.S., Dodson, E., Dodson G., Tolley, S., Turkenburg, J.P., Christiansen, L., Huge-Jensen B., Norskov, L., Thim, L., and Menge, U. (1990) Nature 343, 767-770). Members of the triacylglycerol lipase family share a number of conserved structural features. One such feature is the "GXSXG" motif, in which the central serine residue is one of the three residues comprising the "catalytic triad" (Winkler, K., D'Arcy, A., and Hunziker, W. (1990) Nature 343, 771-77 '4; Faustinella, F., Smith, L.C., and Chan, L. (1992) Biochemistry 31,7219-7223). Conserved aspartate and histidine residues make up the balance of the catalytic triad. A short span of 19-23 amino acids (the "lid region") forms an amphipathic helix structure and covers the catalytic pocket of the enzyme (Winkler, K., D'Arcy, A., and Hunziker, W. (1990) Nature 343, 771-774). This region diverges between members of the family, and it has recently been determined that the span confers substrate specificity to the enzymes (Dugi, K.A., Dichek H.L., and Santamarina-Fojo, S. (1995) J. Biol. Chem. 270, 25396-25401). Comparisons between hepatic and lipoprotein lipase have demonstrated that differences in triacylglycerol lipase and phospholipase activities of the enzymes are in part mediated by this lid region (Dugi, K.A., Dichek H.L., and Santamarina-Fojo, S. (1995) J. Biol. Chem. 270, 25396- 25401 ).

The triacylglycerol lipases possess varying degrees of heparin binding activity. Lipoprotein lipase has the highest affinity for heparin, and this binding activity has been mapped to stretches of positively charged residues in the amino terminal domain (Ma, Y., Henderson, H.E., Liu, M.-S., Zhang, H., Forsythe, I.J., Clarke-Lewis, I., Hayden, M.R., and Brunzell, J.D. J. Lipid Res. 35, 2049-2059). The localization of lipoprotein lipase to the endothelial surface (Cheng, C.F., Oosta, G.M., Bensadoun, A., and Rosenberg, R.D. (1981) J. Biol. Chem. 256, 12893-12896) is primarily mediated through binding to surface proteoglycans (Shimada K., Gill, P.J., Silbert, J.E., Douglas, W.H.J., and Fanburg, B.L. (1981) J. Clin. Invest. 68, 995-1002; Saxena, U., Klein, M.G., and Goldberg, I.J. (1991) J. Biol. Chem. 266, 17516-17521; Eisenberg, S., Sehayek, E., Olivecrona, T., and Vlodavsky, I. (1992) J. Clin Invest. 90,2013-2021). It is this binding activity which allows the enzyme to accelerate LDL uptake by acting as a bridge between LDL and the cell surface (Mulder, M., Lombardi, P., Jansen, H., vanBerkel T.J., Frants R.R., and Havekes, L.M. (1992) Biochem. Biophys. Res. Comm. 185, 582-587; Rutledge, J.C., and Goldberg, I.J., (1994) J. Lipid Res. 35. 1152-1160; Tsuchiya, S., Yamabe, M., Yamaguchi, T., Kobayashi, Y., Konno, T., and Tada, K. (1980) Int. J. Cancer 26,171-176).

Lipoprotein lipase and hepatic lipase are both known to function in conjunction with co- activator proteins: apolipoprotein CII for lipoprotein lipase and colipase for pancreatic lipase. The genetic sequences encoding human pancreatic lipase, hepatic lipase and lipoprotein lipase have been reported (Genbank accession #M93285, #J03540, and #M15856 respectively). The messenger RNAs of human hepatic lipase and pancreatic lipase are approximately 1.7 and 1.8 kilobases in length respectively. Two mRNA transcripts of 3.6 and 3.2 kilobases are produced from the human lipoprotein lipase gene. These two transcripts utilize alternate polyadenylation signals, and differ in their translational efficiency (Ranganathan, G., Ong, J.M., Yukht, A., Saghizadeh, M., Simsolo, R.B., Pauer, A., and Kern, P.A. (1995) J. Biol. Chem. 270, 7149-7155). C) Physiological processes

The metabolism of lipids involves the interaction of lipids, apoproteins, lipoproteins, and enzymes. Hepatic lipase and lipoprotein lipase are multifunctional proteins which mediate the binding, uptake, catabolism, and remodeling of lipoproteins and phospholipids. Lipoprotein lipase and hepatic lipase function while bound to the luminal surface of endothelial cells in peripheral tissues and the liver respectively. Both enzymes participate in reverse cholesterol transport, which is the movement of cholesterol from peripheral tissues to the liver either for excretion from the body or for recycling. Genetic defects in both hepatic lipase and lipoprotein lipase are known to be the cause of familial disorders of lipoprotein metabolism. Defects in the metabolism of lipoproteins result in serious metabolic disorders, including hypercholesterolemia, hyperlipidemia, and atherosclerosis.

Atherosclerosis is a complex, polygenic disease which is defined in histological terms by deposits (lipid or fibrolipid plaques) of lipids and of other blood derivatives in blood vessel walls, especially the large arteries (aorta, coronary arteries, carotid). These plaques, which are more or less calcified according to the degree of progression of the atherosclerotic process, may be coupled with lesions and are associated with the accumulation in the vessels of fatty deposits consisting essentially of cholesterol esters. These plaques are accompanied by a thickening of the vessel wall, hypertrophy of the smooth muscle, appearance of foam cells (lipid-laden cells resulting from uncontrolled uptake of cholesterol by recruited macrophages) and accumulation of fibrous tissue. The atheromatous plaque protrudes markedly from the wall, endowing it with a stenosing character responsible for vascular occlusions by atheroma, thrombosis or embolism, which occur in those patients who are most affected. These lesions can lead to serious cardiovascular pathologies such as infarction, sudden death, cardiac insufficiency, and stroke. The role of triacylglycerol lipases in vascular pathologies such as atherosclerosis has been an area of intense study (reviewed in Olivecrona, G., and Olivecrona, T. (1995) Curr. Opin. Lipid. 6,291-305). Generally, the action of the triacylglycerol lipases is believed to be antiatherogenic because these enzymes lower serum triacylglycerol levels and promote HDL formation. Transgenic animals expressing human lipoprotein lipase or hepatic lipase have decreased levels of plasma triglycerides and an increased level of high density lipoprotein (HDL) (Shimada, M., Shimano, H., Gotoda, T., Yamamoto, K., Kawamura, M., Inaba, T., Yazaki, t., and Yamada, N. (1993) J. Biol. Chem. 268, 17924-17929; Liu, M.-S., Jirik, F. R., LeBoeuf, R. C, Henderson, H., Castellani, L.W., Lusis, A. J., ma, Y., Forsythe, I.J., Zhang, H., Kirk, E., Brunzell, J.D., and Hayden, M.R. (1994) J. Biol. Chem. 269, 11417-11424). Humans with genetic defects resulting in decreased levels of lipoprotein lipase activity have been found to have hypertriglyceridemia but no increased risk of coronary heart disease. This is reported to be due to the lack of production of intermediate-sized, atherogenic lipoproteins which could accumulate within the subendothelial space (Zilversmit, D.B. (1973) Circ. Res. 33,633-638).

In the localized area of an atherosclerotic lesion, however, the increased level of lipase activity is hypothesized to accelerate the atherogenic process (Zilversmit, D.B. (1995) Clin. Chem. 41,153-158; Zambon, A., Torres, A., Bijvoet, S., Gagne, C, Moojani, S., Lupien, P.J., Hayden M.R., and Brunzell, J.D. (1993) Lancet 341, 11 19-1121). This may be due to an increase in the binding and uptake of lipoproteins by vascular tissue mediated by lipases (Eisenberg, S., Sehayek, E., Olivecrona, T. Vlodavsky, I. (1992) J. Clin. Invest. 90,2013-2021; Tabas, I., Li, I., Brocia R.W., Xu, S.W., Swenson T.L. Williams, K.J. (1993) J.Biol. Chem. 268,20419-20432; Nordestgaard, B.G., and Nielsen, A.G. (1994) Curr. Opin. Lipid. 5,252-257; Williams, K.J., and Tabas, I. (1995) Art. Thromb. and Vase. Biol. 15,551-561). Additionally, a high local level of lipase activity may result in cytotoxic levels of fatty acids and lysophosphatidylcholine being produced in precursors of atherosclerotic lesions.

Despite the understanding that has evolved regarding the role of lipase activity in lipid homeostasis, there nevertheless is a need in the art to identify additional genes coding for proteins that regulate lipid metabolism.

SUMMARY OF THE INVENTION

The present invention relates to the discovery of a lipase like gene (LLG), its expressed polypeptide products, and compositions and methods for their use. The LLG polypeptide binds heparin, has homology to human lipoprotein lipase and hepatic lipase, and comprises a 39 kD catalytic domain of the triacylglycerol lipase family. In a further embodiment, the polypeptide has phospholipase A activity.

This invention provides an isolated polypeptide comprising the sequence SEQ ID NO: 10.

This invention further provides an isolated polypeptide comprising the sequence SEQ ID NO: 8 and having an apparent molecular weight of about 55 kD or 68 kD on a 10% SDS-PAGE gel.

This invention also provides an isolated polypeptide comprising the sequence SEQ ID NO: 6 and having an apparent molecular weight of about 40 kD on a 10% SDS-PAGE gel. The invention further provides an antigenic fragment of the LLG polypeptide. Another aspect of this invention is an isolated nucleic acid encoding a polypeptide having the aforesaid sequence.

Another aspect of this invention is a vector comprising the aforesaid nucleic acid encoding said polypeptide operably linked to a regulatory region, such as a promoter.

Another aspect of this invention is a recombinant cell comprising the above-described vector. Another aspect of this invention is a method of preparing a polypeptide which comprises culturing recombinant cells containing said polypeptide encoding nucleic acid under conditions permitting the expression of said polypeptide.

Another aspect of this invention is an antibody which is capable of specifically binding to and/or neutralizing the biological activity of the polypeptides according to the invention. Indeed, a further characteristic of a polypeptide of the invention is that it specifically binds an antibody of the invention, i.e., an antibody specific for an LLG polypeptide.

Another aspect of this invention is a composition comprising a polypeptide, nucleic acid, vector, antisense nucleic acid, or antibody according to the invention and a pharmaceutically acceptable carrier.

Another aspect of this invention is a method of screening for agonists or antagonists of enzymatic activity exhibited by the polypeptides of the present invention comprising contacting potential agonists or antagonists with said polypeptides and a substrate thereof and measuring the ability of the potential agonists or antagonists to enhance or inhibit activity. Another aspect of this invention is a method for the enzymatic hydrolysis of a phosphatidylcholine ester comprising contacting said phosphatidylcholine ester with a polypeptide according to the invention.

Another aspect of this invention is a method of treatment for improving the serum lipid profile of a human or other animal having an undesirable lipid profile comprising administration thereto of an effective amount of a composition according to the invention.

Another aspect of this invention is a method of treating or preventing atherosclerosis in a human or other animal comprising administration thereto of an effective amount of a composition according to the invention.

Other aspects and advantages of the present invention are described further in the drawings and in the following detailed description of the preferred embodiments thereof.

BRIEF DESCRIPTION OF THE DRAWINGS

Figure 1 shows the sequences (SEQ ID Nos: 17-31) of the primers used in the exemplified PCR amplifications. Figure 2 shows the nucleic acid sequence (SEQ ID NO: 1) and the deduced amino acid sequence (SEQ ID NO: 2) of the differential display RT-PCR product containing the lipase like gene cDNA. The sequences corresponding to the two primers used in the amplification are underlined. The termination codon and polyadenylation signal are boxed. The GAATTC motifs and flanking sequence are from the pCRII vector into which the product was cloned. Figure 3 shows the nucleic acid sequence (SEQ ID NO: 3) and the deduced amino acid sequence (SEQ ID NO: 4) of the 5 'RACE extension of the LLG cDNA. The sequences corresponding to the two primers used in the amplification are underlined. The GAATTC motifs and flanking sequence are from the pCRII vector into which the product was cloned.

Figure 4 shows the sequence (SEQ ID NO: 7) of the cDNA containing the complete open reading frame of the lipase like gene, LLGXL. The start codon (ATG) and termination codon (TGA) are boxed. The Dral site (TTTAAA) and Srfl site (GCCCGGGC) used in the construction of the expression vectors are underlined.

Figure 5 shows the deduced amino acid sequence (SEQ ID NO: 8) of the LLGXL protein. The predicted signal sequence is underlined.

Figure 6 shows a protein sequence alignment of the members of the triacylglycerol lipase gene family (SEQ ID Nos: 13-15). Shaded residues are identical to the LLGXL protein (SEQ ID NO: 8). Gaps were introduced into the sequences to maximize the alignment values using the CLUSTAL program.

Figure 7 shows a northern analysis of LLG mRNA in THP-1 cells. Cells were stimulated with either PMA or PMA and oxidized LDL (PMA + oxLDL). Numbers at the left indicate the positions of RNA standards (in kilobases).

Figure 8 shows a northern analysis of mRNAs from multiple human tissues probed with LLG, lipoprotein lipase (LPL) and human beta actin cDNAs. The position of a 4.4 kilobase RNA standard is indicated to the left of the LLG and LPL panels.

Figure 9 shows a northern analysis of LLG and LPL expression in cultured human endothelial cells and THP-1 cells. The cells were either unstimulated (not exposed to PMA) or stimulated with PMA.

Figure 10 shows the sequence of the immunizing peptide (SEQ ID NO: 16) and its relation to the LLGXL protein sequence. The peptide is shown in the shaded box. The terminal cysteine was introduced to aid coupling of the peptide to the carrier protein. Figure 1 1 shows a western analysis of heparin-Sepharose concentrated proteins from conditioned media from cultured endothelial cells. The blot was probed with anti-LLG antiserum. The numbers to the left indicate the positions of protein standards in kilodaltons.

Figure 12 shows a western analysis of heparin-Sepharose bound proteins in conditioned medium from COS-7 cells transiently transfected with an expression vector containing a cDNA for LLGN or LLGXL or no DNA (Mock). Proteins from PMA-stimulated endothelial cells (HCAEC + PMA) were included for size reference. Numbers to the left indicate the apparent molecular weight of the major immunoreactive proteins as determined by a comparison to protein standards.

Figure 13 shows the sequence of the rabbit LLG PCR product (RLLG.SEQ, SEQ ID NO: 12) and the sequence alignment between the rabbit LLG PCR product and the corresponding sequence in the human cDNA (LLG7742A). Identical nucleotides are shaded. Figure 14 shows the phospholipase A activity of human LPL, LLGN, and LLGXL, using a phosphatidylcholine substrate.

Figure 15 shows the triacylglyceride lipase activity of human LPL, LLGN, and LLGXL, using a triolein substrate. Figure 16 shows the hybridization of LLG and LPL probes to genomic DNAs from different species. DETAILED DESCRIPTION OF THE INVENTION

The present invention relates to the discovery of a lipase like gene (LLG) and its expressed polypeptide products. The polypeptide products, members of the triacylglycerol lipase family, comprise an approximately 39 kD catalytic domain of the triacylglycerol lipase family, e,g., having the sequence SEQ ID NO: 10. One embodiment of the present invention is the LLGN polypeptide, which has 354 amino acids. A second embodiment of the present invention is the LLGXL polypeptide, which has 500 amino acids and exhibits 43% similarity to human lipoprotein lipase and 37% similarity to human hepatic lipase. The LLGXL polypeptide has phospholipase A activity.

The inventors isolated a partial cDNA from mRNA of THP-1 cells which had been exposed to phorbol ester and oxidized LDLs. Following a 5' RACE extension of this partial cDNA, the smaller alternately spliced cDNA was isolated. A second, larger cDNA was isolated from a human placental cDNA library. Northern analysis demonstrated that the LLG gene is expressed in endothelial cells.

Antisera raised against a peptide predicted from the open reading frame of the cDNA detected proteins of the predicted sizes for LLGN and LLGXL in conditioned medium from cultured endothelial cells. Treatment of endothelial cells with phorbol esters resulted in increased production of LLG at both the mRNA and protein levels. This is the first member of the triacylglycerol lipase family found to be expressed by endothelial cells.

A) Definitions

The following defined terms are used throughout the present specification and should be helpful in understanding the scope and practice of the present invention. A "polypeptide" is a polymeric compound comprised of covalently linked amino acid residues. Amino acids have the following general structure:

H

R-C-COOH

I NH, SO

Amino acids are classified into seven groups on the basis of the side chain R: (1) aliphatic side chains, (2) side chains containing a hydroxylic (OH) group, (3) side chains containing sulfur atoms, (4) side chains containing an acidic or amide group, (5) side chains containing a basic group, (6) side chains containing an aromatic ring, and (7) proline, an imino acid in which the side chain is fused to the amino group.

A "protein" is a polypeptide which plays a structural or functional role in a living cell. The polypeptides and proteins of the invention may be glycosylated or unglycosylated. "Homology" means similarity of sequence reflecting a common evolutionary origin. Polypeptides or proteins are said to have homology, or similarity, if a substantial number of their amino acids are either (1) identical, or (2) have a chemically similar R side chain. Nucleic acids are said to have homology if a substantial number of their nucleotides are identical.

"Isolated polypeptide" or "isolated protein" is a polypeptide or protein which is substantially free of those compounds that are normally associated therewith in its natural state (e.g., other proteins or polypeptides, nucleic acids, carbohydrates, lipids). "Isolated" is not meant to exclude artificial or synthetic mixtures with other compounds, or the presence of impurities which do not interfere with biological activity, and which may be present, for example, due to incomplete purification, addition of stabilizers, or compounding into a pharmaceutically acceptable preparation.

A molecule is "antigenic" when it is capable of specifically interacting with an antigen recognition molecule of the immune system, such as an immunoglobulin (antibody) or T cell antigen receptor. An antigenic polypeptide contains at least about 5, and preferably at least about 10, amino acids. An antigenic portion of a molecule can be that portion that is immunodominant for antibody or T cell receptor recognition, or it can be a portion used to generate an antibody to the molecule by conjugating the antigenic portion to a carrier molecule for immunization. A molecule that is antigenic need not be itself immunogenic, i.e., capable of eliciting an immune response without a carrier.

"LLGN polypeptide" and "LLGN protein" mean a polypeptide including the sequence SEQ ID NO: 6, said polypeptide being glycosylated or non-glycosylated.

"LLGXL polypeptide" and "LLGXL protein" mean a polypeptide including the sequence SEQ ID NO: 8, said polypeptide being glycosylated or non-glycosylated.

"LLG polypeptide" generically describes both the LLGN polypeptide and the LLGXL polypeptide.

The LLG polypeptide or protein of the invention includes any analogue, fragment, derivative, or mutant which is derived from an LLG polypeptide and which retains at least one biological property of the LLG polypeptide. Different variants of the LLG polypeptide exist in nature. These variants may be allelic variations characterized by differences in the nucleotide sequences of the structural gene coding for the protein, or may involve differential splicing or // post-translational modification. The skilled artisan can produce variants having single or multiple amino acid substitutions, deletions, additions, or replacements. These variants may include, inter alia: (a) variants in which one or more amino acid residues are substituted with conservative or non-conservative amino acids, (b) variants in which one or more amino acids are added to the LLG polypeptide, (c) variants in which one or more of the amino acids includes a substituent group, and (d) variants in which the LLG polypeptide is fused with another polypeptide such as serum albumin. Other LLG polypeptides of the invention include variants in which amino acid residues from one species are substituted for the corresponding residue in another species, either at conserved or non-conserved positions. In another embodiment, amino acid residues at non-conserved positions are substituted with conservative or non-conservative residues. The techniques for obtaining these variants, including genetic (suppressions, deletions, mutations, etc.), chemical, and enzymatic techniques, are known to persons having ordinary skill in the art.

If such allelic variations, analogues, fragments, derivatives, mutants, and modifications, including alternative mRNA splicing forms and alternative post-translational modification forms result in derivatives of the LLG polypeptide which retain any of the biological properties of the LLG polypeptide, they are included within the scope of this invention.

A "nucleic acid" is a polymeric compound comprised of covalently linked subunits called nucleotides. Nucleic acid includes polyribonucleic acid (RNA) and polydeoxyribonucleic acid (DNA), both of which may be single-stranded or double-stranded. DNA includes cDNA, genomic DNA, synthetic DNA, and semi-synthetic DNA. The sequence of nucleotides that encodes a protein is called the sense sequence.

An "antisense nucleic acid" is a sequence of nucleotides that is complementary to the sense sequence. Antisense nucleic acids can be used to down regulate or block the expression of the polypeptide encoded by the sense strand.

"Isolated nucleic acid" means a nucleic acid which is substantially free of those compounds that are normally associated therewith in its natural state. "Isolated" is not meant to exclude artificial or synthetic mixtures with other compounds, or the presence of impurities which do not interfere with biological activity, and which may be present, for example, due to incomplete purification, addition of stabilizers, or compounding into a pharmaceutically acceptable preparation.

The phrase "a nucleic acid which hybridizes at high stringency" means that the hybridized nucleic acids are able to withstand a washing under high stringency conditions. An example of high stringency washing conditions for DNA-DNA hybrids is 0.1X SSC, 0.5% SDS at 68°C. Other conditions of high stringency washing are known to persons having ordinary skill in the art. "Regulatory region" means a nucleic acid sequence which regulates the expression of a nucleic acid. A regulatory region may include sequences which are naturally responsible for expressing a particular nucleic acid (a homologous region) or may include sequences of a different origin (responsible for expressing different proteins or even synthetic proteins). In particular, the sequences can be sequences of eukaryotic or viral genes or derived sequences which stimulate or repress transcription of a gene in a specific or non-specific manner and in an inducible or non-inducible manner. Regulatory regions include origins of replication, RNA splice sites, enhancers, transcriptional termination sequences, signal sequences which direct the polypeptide into the secretory pathways of the target cell, and promoters. A regulatory region from a "heterologous source" is a regulatory region which is not naturally associated with the expressed nucleic acid. Included among the heterologous regulatory regions are regulatory regions from a different species, regulatory regions from a different gene, hybrid regulatory sequences, and regulatory sequences which do not occur in nature, but which are designed by one having ordinary skill in the art. A "vector" is any means for the transfer of a nucleic acid according to the invention into a host cell. The term "vector" includes both viral and nonviral means for introducing the nucleic acid into a prokaryotic or eukaryotic cell in vitro, ex vivo or in vivo. Non-viral vectors include plasmids, liposomes, electrically charged lipids (cytofectins), DNA-protein complexes, and biopolymers. Viral vectors include retrovirus, adeno-associated virus, pox, baculovirus, vaccinia, herpes simplex, Epstein-Barr and adenovirus vectors. In addition to nucleic acid according to the invention, a vector may also contain one or more regulatory regions, and/or selectable markers useful in selecting, measuring, and monitoring nucleic acid transfer results (transfer to which tissues, duration of expression, etc.).

A "recombinant cell" is a cell which contains a nucleic acid which is not naturally present in the cell. "Recombinant cell" includes higher eukaryotic cells such as mammalian cells, lower eukaryotic cells such as yeast cells, prokaryotic cells, and archaebacterial cells.

"Pharmaceutically acceptable carrier" includes diluents and fillers which are pharmaceutically acceptable for method of administration, are sterile, and may be aqueous or oleaginous suspensions formulated using suitable dispersing or wetting agents and suspending agents. The particular pharmaceutically acceptable carrier and the ratio of active compound to carrier are determined by the solubility and chemical properties of the composition, the particular mode of administration, and standard pharmaceutical practice.

A "lipase" is a protein which can enzymatically cleave a lipid substrate.

A "phospholipase" is a protein which can enzymatically cleave a phospholipid substrate. A "triacylglycerol lipase" is a protein which can enzymatically cleave a triacylglyceride substrate. x3

'Phosphatidylcholine" is a glycerol phospholipid having the following structure:

R and R' are the hydrocarbon side chains of fatty acids. Phosphatidylcholine is also known as lecithin. "Lipid profile" means the set of concentrations of cholesterol, triglyceride, lipoprotein cholesterol and other lipids in the body of a human or other animal.

An "undesirable lipid profile" is the condition in which the concentrations of cholesterol, triglyceride, or lipoprotein cholesterol are outside of the age- and gender-adjusted reference ranges. Generally, a concentration of total cholesterol > 200 mg/dl, of plasma triglycerides > 200 mg/dl, of LDL cholesterol > 130 mg/dl, of HDL cholesterol < 39 mg/dl, or a ratio of total cholesterol to HDL cholesterol > 4.0 is considered to be an undesirable lipid profile. An undesirable lipid profile is associated with a variety of pathological conditions, including hyperlipidaemias, diabetes hypercholesterolaemia, atherosclerosis, and other forms of coronary artery disease.

B) Polypeptides

The present invention provides polypeptides which are members of the triacylglycerol lipase family, and which comprise a 39 kD catalytic domain of the triacylglycerol lipase family, e.g., having the sequence SEQ ID NO: 10. One embodiment of the present invention is an isolated LLG polypeptide comprising the sequence SEQ ID NO: 6 and having an apparent molecular weight of about 40 kD on a 10% SDS-PAGE gel. Another embodiment of the present invention is an isolated LLG polypeptide comprising the sequence SEQ ID NO: 8 and having an apparent molecular weight of about 55 kD or 68 kD on a 10% SDS-PAGE gel. The polypeptides and proteins of the present invention may be recombinant polypeptides, natural polypeptides, or synthetic polypeptides, and may be of human, rabbit, or other animal origin. The polypeptides are characterized by a reproducible single molecular weight and/or multiple set of molecular weights, chromatographic response and elution profiles, amino acid composition and sequence, and biological activity. The polypeptides of the present invention may be isolated from natural sources, such as placental extracts, human plasma, or conditioned media from cultured cells such as macrophages or endothelial cells, by using the purification procedures known to one of skill in the art.

Alternatively, the polypeptides of the present invention may be prepared utilizing recombinant DNA technology, which comprises combining a nucleic acid encoding the polypeptide thereof in a suitable vector, inserting the resulting vector into a suitable host cell, recovering the polypeptide produced by the resulting host cell, and purifying the polypeptide recovered.

C) Nucleic Acids

The present invention provides isolated nucleic acids which encode LLG polypeptides. The present invention also provides antisense nucleic acids which can be used to down regulate or block the expression of LLG polypeptides in vitro, ex vivo or in vivo.

The techniques of recombinant DNA technology are known to those of ordinary skill in the art. General methods for the cloning and expression of recombinant molecules are described in Maniatis (Molecular Cloning, Cold Spring Harbor Laboratories, 1982), and in Ausubel (Current Protocols in Molecular Biology, Wiley and Sons, 1987), which are incorporated by reference.

The nucleic acids of the present invention may be linked to one or more regulatory regions. Selection of the appropriate regulatory region or regions is a routine matter, within the level of ordinary skill in the art. Regulatory regions include promoters, and may include enhancers, suppressors, etc..

Promoters that may be used in the present invention include both constituitive promoters and regulated (inducible) promoters. The promoters may be prokaryotic or eukaryotic depending on the host. Among the prokaryotic (including bacteriophage) promoters useful for practice of this invention are lad, lacZ, T3, T7, lambda P_r, P„ and trp promoters. Among the eukaryotic (including viral) promoters useful for practice of this invention are ubiquitous promoters (e.g. HPRT, vimentin, actin, tubulin), intermediate filament promoters (e.g. desmin, neurofilaments, keratin, GFAP), therapeutic gene promoters (e.g. MDR type, CFTR, factor VIII), tissue-specific promoters (e.g. actin promoter in smooth muscle cells, or Fit and Flk promoters active in endothelial cells), promoters which are preferentially activated in dividing cells, promoters which respond to a stimulus (e.g. steroid hormone receptor, retinoic acid receptor), tetracycline-regulated transcriptional modulators, cytomegalovirus immediate-early, retroviral LTR, metallothionein, SV-40, El a, and MLP promoters. Tetracycline-regulated transcriptional modulators and CMV promoters are described in WO 96/01313, US 5,168,062 and 5,385,839, the contents of which are incorporated herein by reference. S^"

Preferably, the viral vectors used in gene therapy are replication defective, that is, they are unable to replicate autonomously in the target cell. In general, the genome of the replication defective viral vectors which are used within the scope of the present invention lack at least one region which is necessary for the replication of the virus in the infected cell. These regions can either be eliminated (in whole or in part), be rendered non-functional by any technique known to a person skilled in the art. These techniques include the total removal, substitution (by other sequences, in particular by the inserted nucleic acid), partial deletion or addition of one or more bases to an essential (for replication) region. Such techniques may be performed in vitro (on the isolated DNA) or in situ, using the techniques of genetic manipulation or by treatment with mutagenic agents.

Preferably, the replication defective virus retains the sequences of its genome which are necessary for encapsidating the viral particles.

The retroviruses are integrating viruses which infect dividing cells. The retrovirus genome includes two LTRs, an encapsidation sequence and three coding regions (gag, pol and env). The construction of recombinant retroviral vectors has been described: see, in particular, EP 453242, EP178220, Bernstein et al. Genet. Eng. 7 (1985) 235; McCormick, BioTechnology 3 (1985) 689, etc. In recombinant retroviral vectors, the gag, pol and env genes are generally deleted, in whole or in part, and replaced with a heterologous nucleic acid sequence of interest. These vectors can be constructed from different types of retrovirus, such as, MoMuLV ("murine Moloney leukaemia virus" MSV ("murine Moloney sarcoma virus"), HaSV ("Harvey sarcoma virus"); SNV ("spleen necrosis virus"); RSV ("Rous sarcoma virus") and Friend virus.

In general, in order to construct recombinant retroviruses containing a sequence encoding LLG according to the invention, a plasmid is constructed which contains the LTRs, the encapsidation sequence and the coding sequence. This construct is used to transfect a packaging cell line, which cell line is able to supply in trans the retroviral functions which are deficient in the plasmid. In general, the packaging cell lines are thus able to express the gag, pol and env genes. Such packaging cell lines have been described in the prior art, in particular the cell line PA317 (US4.861.719); the PsiCRIP cell line (WO90/02806) and the GP+envAm-12 cell line (WO89/07150). In addition, the recombinant retroviral vectors can contain modifications within the LTRs for suppressing transcriptional activity as well as extensive encapsidation sequences which may include a part of the gag gene (Bender et al., J. Virol. 61 (1987) 1639). Recombinant retroviral vectors are purified by standard techniques known to those having ordinary skill in the art.

The adeno-associated viruses (AAV) are DNA viruses of relatively small size which can integrate, in a stable and site-specific manner, into the genome of the cells which they infect.

They are able to infect a wide spectrum of cells without inducing any effects on cellular growth, morphology or differentiation, and they do not appear to be involved in human pathologies. The AAV genome has been cloned, sequenced and characterized. It encompasses approximately 4700 bases and contains an inverted terminal repeat (ITR) region of approximately 145 bases at each end, which serves as an origin of replication for the virus. The remainder of the genome is divided into two essential regions which carry the encapsidation functions: the left-hand part of the genome, which contains the rep gene involved in viral replication and expression of the viral genes; and the right-hand part of the genome, which contains the cap gene encoding the capsid proteins of the virus.

The use of vectors derived from the AAVs for transferring genes in vitro and in vivo has been described (see WO 91/18088; WO 93/09239; US 4,797,368, US 5,139,941, EP 488 528). These publications describe various AAV-derived constructs in which the rep and/or cap genes are deleted and replaced by a gene of interest, and the use of these constructs for transferring the said gene of interest in vitro (into cultured cells) or in vivo, (directly into an organism). The replication defective recombinant AAVs according to the invention can be prepared by cotransfecting a plasmid containing the nucleic acid sequence of interest flanked by two AAV inverted terminal repeat (ITR) regions, and a plasmid carrying the AAV encapsidation genes

(rep and cap genes), into a cell line which is infected with a human helper virus (for example an adenovirus). The AAV recombinants which are produced are then purified by standard techniques. The invention also relates, therefore, to an AAV-derived recombinant virus whose genome encompasses a sequence encoding an LLG polypeptide flanked by the AAV ITRs. The invention also relates to a plasmid encompassing a sequence encoding an LLG polypeptide flanked by two ITRs from an AAV. Such a plasmid can be used as it is for transferring the LLG sequence, with the plasmid, where appropriate, being incorporated into a liposomal vector (pseudo- virus).

In a preferred embodiment, the vector is an adenovirus vector. Adenoviruses are eukaryotic DNA viruses that can be modified to efficiently deliver a nucleic acid of the invention to a variety of cell types.

Various serotypes of adenovirus exist. Of these serotypes, preference is given, within the scope of the present invention, to using type 2 or type 5 human adenoviruses (Ad 2 or Ad 5) or adenoviruses of animal origin (see W094/26914). Those adenoviruses of animal origin which can be used within the scope of the present invention include adenoviruses of canine, bovine, murine (example: Mavl, Beard et al., Virology 75 (1990) 81), ovine, porcine, avian, and simian (example: SAV) origin. Preferably, the adenovirus of animal origin is a canine adenovirus, more preferably a CAV2 adenovirus (e.g. Manhattan or A26/61 strain (ATCC VR-800), for example). Preferably, the replication defective adenoviral vectors of the invention comprise the ITRs, an encapsidation sequence and the nucleic acid of interest. Still more preferably, at least the El region of the adenoviral vector is non-functional. The deletion in the El region preferably extends from nucleotides 455 to 3329 in the sequence of the Ad5 adenovirus. Other /7 regions may also be modified, in particular the E3 region (WO95/02697), the E2 region (W094/28938), the E4 region (W094/28152, W094/12649 and WO95/02697), or in any of the late genes L1-L5. Defective retroviral vectors are disclosed in WO95/02697.

In a preferred embodiment, the adenoviral vector has a deletion in the El and E4 regions. In another preferred embodiment, the adenoviral vector has a deletion in the El region into which the E4 region and the sequence encoding LLG are inserted (see FR94 13355).

The replication defective recombinant adenoviruses according to the invention can be prepared by any technique known to the person skilled in the art (Levrero et al., Gene 101 (1991) 195, EP 185 573; Graham, EMBO J. 3 (1984) 2917). In particular, they can be prepared by homologous recombination between an adenovirus and a plasmid which carries, inter alia, the DNA sequence of interest. The homologous recombination is effected following cotransfection of the said adenovirus and plasmid into an appropriate cell line. The cell line which is employed should preferably (i) be transformable by the said elements, and (ii) contain the sequences which are able to complement the part of the genome of the replication defective adenovirus, preferably in integrated form in order to avoid the risks of recombination. Examples of cell lines which may be used are the human embryonic kidney cell line 293 (Graham et al., J. Gen. Virol. 36 (1977) 59) which contains the left-hand portion of the genome of an Ad5 adenovirus (12%) integrated into its genome, and cell lines which are able to complement the El and E4 functions, as described in applications W094/26914 and WO95/02697. Recombinant adenoviruses are recovered and purified using standard molecular biological techniques, which are well known to one of ordinary skill in the art.

The down regulation of gene expression using antisense nucleic acids can be achieved at the translational or transcriptional level. Antisense nucleic acids of the invention are preferably nucleic acid fragments capable of specifically hybridizing with all or part of a nucleic acid encoding LLG or the corresponding messenger RNA. These antisense nucleic acids can be synthetic oligonucleotides, optionally modified to improve their stability and selectivity. They can also be DNA sequences whose expression in the cell produces RNA complementary to all or part of the LLG mRNA. Antisense nucleic acids can be prepared by expression of all or part of a sequence selected from the group consisting of SEQ ID No. 2, SEQ ID No. 3, SEQ ID No. 7, or SEQ ID No. 1 1, in the opposite orientation, as described in EP 140308. Any length of antisense sequence is suitable for practice of the invention so long as it is capable of down- regulating or blocking expression of LLG. Preferably, the antisense sequence is at least 20 nucleotides in length. The preparation and use of antisense nucleic acids, DNA encoding antisense RNAs and the use of oligo and genetic antisense is disclosed in WO92/15680, the contents of which are incorporated herein by reference. D) Antibodies Sf

The present invention provides antibodies against the LLG polypeptide. These antibodies may be monoclonal antibodies or polyclonal antibodies. The present invention includes chimeric, single chain, and humanized antibodies, as well as Fab fragments and the products of an Fab expression library.

Polyclonal antibodies may be prepared against an antigenic fragment of an LLG polypeptide, as described in Example 4A. Antibodies may also be generated against the intact LLG protein or polypeptide, or against a fragment, derivative, or epitope of the protein or polypeptide. Antibodies may be obtained following the administration of the protein, polypeptide, fragment, derivative, or epitope to an animal, using the techniques and procedures known in the art.

Monoclonal antibodies may be prepared using the method of Mishell, B. B., et al., Selected Methods In Cellular Immunology, (W.H. Freeman, ed.) San Francisco (1980). Briefly, a polypeptide of the present invention is used to immunize spleen cells of Balb/C mice. The immunized spleen cells are fused with myeloma cells. Fused cells containing spleen and myeloma cell characteristics are isolated by growth in HAT medium, a medium which kills both parental cells, but allows the fused products to survive and grow.

The monoclonal antibodies of the present invention may be "humanized" to prevent the host from mounting an immune response to the antibodies. A "humanized antibody" is one in which the complementarity determining regions (CDRs) and/or other portions of the light and/or heavy variable domain framework are derived from a non-human immunoglobulin, but the remaining portions of the molecule are derived from one or more human immunoglobulins. Humanized antibodies also include antibodies characterized by a humanized heavy chain associated with a donor or acceptor unmodified light chain or a chimeric light chain, or vice versa. The humanization of antibodies may be accomplished by methods known in the art (see, e.g. G.E. Mark and E.A. Padlan, "Chapter 4. Humanization of Monoclonal Antibodies", The Handbook of Experimental Pharmacology Vol. 113, Springer- Verlag, New York, 1994). Transgenic animals may be used to express humanized antibodies.

Techniques known in the art for the production of single chain antibodies can be adapted to produce single chain antibodies to the immunogenic polypeptides and proteins of the present invention.

The anti-LLG antibodies are useful in assays for detecting or quantitating levels of LLG. In one embodiment, these assays provide a clinical diagnosis and assessment of LLG in various disease states and a method for monitoring treatment efficacy.

E) Methods of Screening for Agonists or Antagonists /9

The present invention provides methods of screening small molecule libraries or natural product sources for agonists (enhancers or co-activators including proteinaceous co-activators) or antagonists (inhibitors) of LLGXL activity. A potential agonist or antagonist is contacted with

LLGXL protein and a substrate of LLGXL, and the ability of the potential agonist or antagonist to enhance or inhibit LLGXL activity is measured.

The LLGXL protein used in the method can be produced recombinantly in a variety of host cells, including mammalian cells (as shown in Example 7), baculovirus-infected insect cells, yeast, and bacteria. LLG expression in stably transfected CHO cells can be optimized by methotrexate amplification of the cells. LLGXL protein can also be purified from natural sources such as human plasma, placental extracts, or conditioned media from cultured endothelial cells, THP-1 cells, or macrophages.

The optimization of assay parameters including pH, ion concentrations, temperature, concentration of substrate, and emulsification conditions are determined empirically by one having ordinary skill in the art. The fatty acid substituents of the substrates may vary in chain length as well as in degree and position of unsaturation. The substrates may be radiolabelled in any of several positions.

Phospholipid substrates such as phosphatidylcholine can be radiolabelled, for example, in the

Sn-1 or Sn-2 fatty acid position, or in the glycerol, phosphate, or polar head group (choline in the case of phosphatidylcholine). As an alternative to radiolabeled substrates, other classes of labeled substrates, such as fluorescent substrates or thio-containing substrates, can also be used in the screening methods. Fluorescent substrates are particularly useful in screening assays because enzymatic catalysis can be measured continuously by measuring fluorescence intensity, without the physical separation (extraction) of the products from the substrates. An example of a fluorescent phosphatidylcholine substrate is C₆NBD-PC{ l-acyl-2-[6-(nitro-2,l,3-benzoxadiazol-

4-yl)amino] caproylphosphatidylcholine.

The thio-containing substrates include l,2-bis(hexanoylthio)-l ,2-dideoxy-sn-glycero-3- phosphorylcholine (L.J. Reynolds, W.N. Washburn, R.A. Deems, and E.A. Dennis, 1991.

Methods in Enzymology 197: 3-23; L. Yu and E.A. Dennis, 1991. Methods in Enzymology 197: 65-75; L.A. Wittenauer, K. Shirai, R.L. Jackson, and J.D. Johnson, 1984. Biochem.

Biophys. Res. Commun. 1 18: 894-901).

F) Hydrolysis of Phosphatidylcholine Esters

The present invention provides a method for the enzymatic hydrolysis of phosphatidylcholine esters, e.g., for industrial or food processing, or in laundry detergents. The polypeptides of the present invention can be used to hydrolyze phosphatidylcholine esters in o2_<g> solution, or the enzymes may be bound to a solid support which is then contacted with the substrate. This method can be used to produce lysophospholipids and free fatty acids.

G) Compositions The present invention provides compositions in a biologically compatible

(biocompatible) solution, comprising the polypeptides, nucleic acids, vectors, and antibodies of the invention. A biologically compatible solution is a solution in which the polypeptide, nucleic acid, vector, or antibody of the invention is maintained in an active form, e.g., in a form able to effect a biological activity. For example, a polypeptide of the invention would have phospholipase activity, a nucleic acid would be able to replicate, translate a message, or hybridize to a complementary nucleic acid; a vector would be able to transfect a target cell; an antibody would bind a polypeptide of the invention. Generally, such a biologically compatible solution will be an aqueous buffer, e.g., Tris, phosphate, or HEPES buffer, containing salt ions. Usually the concentration of salt ions will be similar to physiological levels. In a specific embodiment, the biocompatible solution is a pharmaceutically acceptable composition. Biologically compatible solutions may include stabilizing agents and preservatives.

Such compositions can be formulated for administration by topical, oral, parenteral, intranasal, subcutaneous, and intraocular, routes. Parenteral administration is meant to include intravenous injection, intramuscular injection, intraarterial injection or infusion techniques. The composition may be administered parenterally in dosage unit formulations containing standard, well known nontoxic physiologically acceptable carriers, adjuvants and vehicles as desired.

The preferred sterile injectable preparations can be a solution or suspension in a nontoxic parenterally acceptable solvent or diluent. Examples of pharmaceutically acceptable carriers are saline, buffered saline, isotonic saline (e.g. monosodium or disodium phosphate, sodium, potassium, calcium or magnesium chloride, or mixtures of such salts), Ringer's solution, dextrose, water, sterile water, glycerol, ethanol, and combinations thereof. 1 ,3-butanediol and sterile fixed oils are conveniently employed as solvents or suspending media. Any bland fixed oil can be employed including synthetic mono- or di-glycerides. Fatty acids such as oleic acid also find use in the preparation of injectables. The composition medium can also be a hydrogel which is prepared from any biocompatible or non-cytotoxic (homo or hetero) polymer, such as a hydrophilic polyacrylic acid polymer that can act as a drug absorbing sponge. Such polymers have been described, for example, in application WO93/08845, the entire contents of which are hereby incorporated by reference. Certain of them, such as, in particular, those obtained from ethylene and/or propylene oxide are commercially available. A hydrogel can be deposited directly onto the surface of the tissue to be treated, for example during surgical intervention. Another preferred embodiment of the present invention relates to a pharmaceutical composition comprising a replication defective recombinant virus and poloxamer. More specifically, the invention relates to a composition comprising a replication defective recombinant virus comprising a nucleic acid encoding an LLG polypeptide and poloxamer. A preferred poloxamer is Poloxamer 407, which is commercially available (BASF, Parsippany, NJ) and is a non-toxic, biocompatible polyol, and is most preferred. A poloxamer impregnated with recombinant viruses may be deposited directly on the surface of the tissue to be treated, for example during a surgical intervention. Poloxamer possesses essentially the same advantages as hydrogel while having a lower viscosity.

H) Methods of Treatment

The present invention provides methods of treatment which comprise the administration to a human or other animal of an effective amount of a composition of the invention.

Effective amounts may vary, depending on the age, type and severity of the condition to be treated, body weight, desired duration of treatment, method of administration, and other parameters. Effective amounts are determined by a physician or other qualified medical professional.

Polypeptides according to the invention are generally administered in doses of about 0.01 mg/kg to about 100 mg/kg, preferably about 0.1 mg/kg to about 50 mg/kg, and most preferably about 1 mg/kg to about 10 mg/kg of body weight per day.

Recombinant viruses according to the invention are generally formulated and administered in the form of doses of between about 10⁴ and about 10'⁴ pfu. In the case of AAVs and adenoviruses, doses of from about 10⁶ to about 10" pfu are preferably used. The term pfu ("plaque-forming unit") corresponds to the infective power of a suspension of virions and is determined by infecting an appropriate cell culture and measuring the number of plaques formed. The techniques for determining the pfu titre of a viral solution are well documented in the prior art.

The present invention provides methods of treating atherosclerosis wherein said atherosclerosis is the result of excess, abnormal or inadequate expression of LLG polypeptide activity.

The present invention further provides methods of treating a human or other animal having an undesirable lipid profile, wherein said undesirable lipid profile is the result of abnormally high or inadequate expression of LLG polypeptide activity.

The present invention further provides methods of treating diabetes, hyperlipidemia, intrahepatic cholestasis or other metabolic disorders wherein said diabetes, hyperlipidemia, intrahepatic cholestasis or other metabolic disorder is the result of abnormally high or inadequate expression of LLG polypeptide activity. 1) Treatment of Undersirable Lipid Profiles Associated With Increased Expression of LLG Polypeptide

The methods for decreasing the expression of LLG polypeptide to correct those conditions in which LLG polypeptide activity contributes to a disease or disorder associated with an undesriable lipid profile include but are not limited to administration of a composition comprising an antisense nucleic acid, administration of a composition comprising an intracellular binding protein such as an antibody, administration of a composition comprising the LLGN polypeptide or another fragment of LLG and administration of a composition comprising a nucleic acid which encodes the LLGN polypeptide or another fragment of LLG. In one embodiment, a composition comprising an antisense nucleic acid is used to down- regulate or block the expression of LLG. In one preferred embodiment, the nucleic acid encodes antisense RNA molecules. In this embodiment, the nucleic acid is operably linked to signals enabling expression of the nucleic acid sequence and is introduced into a cell utilizing, preferably, recombinant vector constructs, which will express the antisense nucleic acid once the vector is introduced into the cell. Examples of suitable vectors includes plasmids, adenoviruses, adeno-associated viruses, retroviruses, and herpes viruses. Preferably, the vector is an adenovirus. Most preferably, the vector is a replication defective adenovirus comprising a deletion in the El and/or E3 regions of the virus.

In another embodiment, the expression of LLG is down-regulated or blocked by the expression of a nucleic acid sequence encoding an intracellular binding protein which is capable of selectively interacting with LLG. WO 94/29446 and WO 94/02610, the contents of which are incorporated herein by reference, disclose cellular transfection with genes encoding an intracellular binding protein. An intracellular binding protein includes any protein capable of selectively interacting, or binding, with LLG in the cell in which it is expressed and of neutralizing the function of bound LLG. Preferably, the intracellular binding protein is an antibody or a fragment of an antibody. More preferably, the intracellular binding protein is a single chain antibody.

WO 94/02610 discloses preparation of antibodies and identification of the nucleic acid encoding a particular antibody. Using LLG or a fragment thereof, a specific monoclonal antibody is prepared by techniques known to those skilled in the art. A vector comprising the nucleic acid encoding an intracellular binding protein, or a portion thereof, and capable of expression in a host cell is subsequently prepared for use in the method of this invention.

Alternatively, LLG activity can be blocked by administration of a neutralizing antibody into the circulation. Such a neutralizing antibody can be administered directly as a protein, or it can be expressed from a vector (with a secretory signal).

In another embodiment, LLGXL activity is inhibited by the administration of a composition comprising the LLGN polypeptide or another fragment of LLG. This composition o£v£ may be administered in a convenient manner, such as by the oral, topical, intravenous, intraperitoneal, intramuscular, subcutaneous, intranasal, or intradermal routes. The composition may be administered directly or it may be encapsulated (e.g. in a lipid system, in amino acid microspheres, or in globular dendrimers). The polypeptide may, in some cases, be attached to another polymer such as serum albumin or polyvinyl pyrrolidone.

In another embodiment, LLGXL activity is inhibited through the use of small molecular weight compounds, which interfere with its enzymatic properties or prevent its appropriate recognition by cellular binding sites.

In another embodiment, LLGXL activity is inhibited through the use of gene therapy, that is, through the administration of a composition comprising a nucleic acid which encodes and directs the expression of the LLGN polypeptide or another fragment of LLG.

In a specific embodiment, the LLG gene of the present invention also has an affinity for heparin. LLG polypeptide binding to extracellular heparin in the lumen of blood vessels would permit LLG to bind to and accelerate LDL uptake by acting as a bridge between LDL and the extracellular heparin. In the localized area of an atherosclerotic lesion, an increased level of lipase activity is hypothesized to accelerate the atherogenic process (Zilversmit, D.B. (1995) Clin. Chem. 41,153-158; Zambon, A., Torres, A., Bijvoet, S., Gagne, C, Moojani, S., Lupien, P.J., Hayden M.R., and Brunzell, J.D. (1993) Lancet 341, 1 119-1121). This may be due to an increase in the binding and uptake of lipoproteins by vascular tissue mediated by lipases (Eisenberg, S., Sehayek, E., Olivecrona, T. Vlodavsky, I. (1992) J. Clin. Invest. 90,2013-2021; Tabas, I., Li, I., Brocia R.W., Xu, S.W., Swenson T.L. Williams, K.J. (1993) J.BioI. Chem. 268,20419-20432; Nordestgaard, B.G., and Nielsen, A.G. (1994) Curr. Opin. Lipid. 5,252-257; Williams, K.J., and Tabas, I. (1995) Art. Thromb. and Vase. Biol. 15,551-561 ). Additionally, a high local level of lipase activity may result in cytotoxic levels of fatty acids and lysophosphatidylcholine being produced in precursors of atherosclerotic lesions. This particular activity of LLG may contribute to the development or progression of atherosclerosis, particularly in the context of excessive lipid levels in a subject due to dietary or genetic factors. Thus, the present invention permits inhibition of lipoprotein accumulation by inhibiting LLG polypeptide expression or binding to lipoprotein (e.g., LDL). 2) Treatment of Undesirable Lipid Profiles Associated With Insufficient LLG

Polypeptide Activity

The methods for increasing the expression of LLG to correct those conditions in which LLG polypeptide activity contributes to a disease or disorder associated with an undesirable lipid profile include but are not limited to administration of a composition comprising the LLGXL polypeptide and administration of a composition comprising a nucleic acid which encodes the LLGXL polypeptide. In one embodiment, the level of LLGXL activity is increased through the administration of a composition comprising the LLGXL polypeptide. This composition may be administered in a convenient manner, such as by the oral, topical, intravenous, intraperitoneal, intramuscular, subcutaneous, intranasal, or intradermal routes. The composition may be administered directly or it may be encapsulated (e.g. in a lipid system, in amino acid microspheres, or in globular dendrimers). The polypeptide may, in some cases, be attached to another polymer such as serum albumin or polyvinyl pyrrolidone.

In another embodiment, the level of LLGXL is increased through the use of small molecular weight compounds, which can upregulate LLGXL expression at the level of transcription, translation, or post-translation.

In another embodiment, the level of LLGXL is increased through the use of gene therapy, that is, through the administration of composition comprising a nucleic acid which encodes and directs the expression of the LLGXL polypeptide.

Intrahepatic cholestasis can be characterized by increased serum cholesterol and phospholipid levels. A recently described, phalloidin drug-induced intrahepatic cholestasis model in rats demonstrated significant increases in the serum levels of cholesterol and phospholipid (Ishizaki, K., Kinbara, S., Miyazawa, N., Takeuchi, Y., Hirabayashi, N., Kasai, H., and Araki, T. (1997) Toxicol. Letters 90, 29-34). The products of this invention may be used to treat intrahepatic cholestasis in patients that have increased serum cholesterol and/or phospholipid. In addition, this rat model also exhibited a severe decrease in biliary cholesterol excretion rates. The LLG polypeptide and nucleic acid products of this invention may be used to treat patients with an impaired biliary excretion system.

Intrahepatic cholestasis is also characterized by impaired bile flow from the liver. Recently, the loci for progressive familial intrahepatic cholestasis (PFIC or Byler disease) and benign recurrent intrahepatic cholestasis (BRIC) were mapped to 18q21-q22 (Carlton, V.E.H., Knisely, A.S., and Freimer, N.B. (1995) Hum. Mol. Genet. 4, 1049-1053 and Houwen, R.H., Baharloo, S., Blankenship, K., Raeymaekers, P., Juyn, J., Sandkuijl, L.A., and Freimer, N.B. (1994) Nature Genet. 8, 380-386, respectively). As LLG gene maps within this chromosomal region at 18q21, the LLG gene or products of this invention may be used to treat patients with intrahepatic cholestasis that is caused by a mutation or defective expression of the PFIC/BRIC disease gene(s).

In another embodiment, the LLG gene or polypeptide products of this invention may be used to treat patients with intrahepatic cholestasis that is not due to a defect in the PFIC/BRIC disease gene(s) at 18q21-q22. A recent study suggested that another locus, located outside of the 18q21-q22 region may also produce the PFIC phenotype (Strautnieks, S.S., Kagalwalla, A.F., Tanner, M.S., Gardiner, R.M., and Thompson, R.J. (1996) J. Med. Genet. 33, 833-836). Nevertheless, administration of LLG polypeptide, either directly or via gene therapy, may alleviate this form of the condition.

In gene therapy one or more nucleic acids encoding a polypeptide, as well as regulatory regions controlling their expression, are transferred into the target cells of a human or other animal. This transfer is carried out either ex vivo in a procedure in which the nucleic acid is transferred to cells in the laboratory and the modified cells are then administered to the human or other animal, or in vivo in a procedure in which the nucleic acid is transferred directly to cells within the human or other animal. The transfer of nucleic acids can be achieved using either the viral or non-viral vectors described above. Non-viral vectors may be transferred into cells using any of the methods known in the art, including calcium phosphate coprecipitation, lipofection (synthetic anionic and cationic liposomes), receptor-mediated gene delivery, naked DNA injection, electroporation and bioballistic or particle acceleration.

EXAMPLES

The following examples illustrate the invention. These examples are illustrative only, and do not limit the scope of the invention.

EXAMPLE 1 Identification of a Differentially Expressed cDNA

A) RNA Preparation

Human monocytic THP-1 cells (Smith, P.K., Krohn, R.I., Hermanson, G.T., Mallia, A.K., Gartner, F.H. Provenzano, M.D., Fujimoto, E.K., Goeke, N.M., Olson, B.J., and Klenk, D.C. (1985) Anal. Biochem. 150,76-85) were cultured in RPMI-1640 medium (GIBCO) with 25 mM HEPES, 10% fetal bovine serum, 100 units/ml penicillin G sodium and 100 units/ml streptomycin sulfate. Cells were plated onto 15 cm tissue culture dishes at 1.5xl0⁷ cells/plate, and treated with 40 ng/ml phorbol 12-myristate 13-acetate (Sigma) for 48 hours to induce differentiation of the cells. Human low density lipoproteins (LDL) were purchased from Calbiochem, and were dialyzed exhaustively versus PBS at 4°C. The LDL was then diluted to 500μg/ml and dialyzed versus 5μM CuS0₄ in PBS at 37"C for 16 hours. To stop oxidation, the LDL was dialyzed exhaustively versus 150 M NaCl, 0.3 mM EDTA, then filter sterilized. Protein concentration was determined by the BCA method (Schuh, J. Fairclough, G.F., and Haschemeyer, R.H. (1978) Proc. Natl. Acad. Sci. USA 75, 3173-3177) (Pierce). The degree of oxidation was determined by TBARS (Chomczynski, P. (1993) Biotechniques 15,532-537), and was between 25-30 nmol MDA equivalents/mg protein. The differentiated THP-l cells were exposed for 24 hours to either 50 μg/ml oxidized LDL or NaCl-EDTA buffer in RPMI medium with 10% lipoprotein-deficient fetal bovine serum (Sigma). To harvest the RNA, the plates were rinsed with 10 ml of PBS, then 14 ml of TRIZOL (Liang, P. and Pardee, A.B. (1992) Science 257,967-971 ) (GIBCO) were added to each plate. The solution was pipetted several times to mix, then like samples were pooled into centrifuge tubes and 3 ml chloroform per plate were added and mixed. The tubes were centrifuged for 15 minutes at 12000 x g. After centrifugation the upper layer was transferred to a new tube and 7.5 ml isopropanol per plate was added and mixed. The tubes were centrifuged at 12000 x g for 20 minutes. The pellet was rinsed with ice-cold 70% ethanol and dried at room temperature. The pellets were suspended in 500 μl TE (Tris-EDTA) and treated with 200 units RNase-free DNAse I and 200 units RNasin placental RNase inhibitor (Promega) for 30 minutes at 37°C. The RNA was purified by sequential extractions with phenol, phenol/chloroform/isoamyl alcohol (25:24: 1), and chloroform/isoamyl alcohol (24: 1 ) followed by ethanol precipitation.

B) cDNA Synthesis cDNA synthesis and PCR amplification were accomplished using protocols from the

Differential Display Kit, version 1.0 (Display Systems Biotechnology, Inc.) This system is based on the technique originally described by Liang and Pardee (Mead, D.A., Pey, N.K., Herrnstadt, C, Marcil, R.A., and Smith, L.M., (1991) Bio/Technology 9,657-663). The primer pairs which yielded the cDNA fragment containing the first information of the lipase like gene were downstream primer 7 and upstream primer 15. The cDNA for the amplification was synthesized as follows, using RNA derived from PMA treated THP-1 cells exposed to either buffer or oxidized LDL: 3 μl of 25 μM downstream primer 7 and 7.5 μl of diethylpyrocarbonate (DEPC)-treated water were added to 300 ng (3.0 μl) RNA from either sample of THP-1 RNA. This was heated to 70"C for 10 minutes then chilled on ice. To this tube were added 3 μl of 5x PCR buffer (250 mM Tris-HCl pH 8.3, 375 mM KCl)(GIBCO), 3 μl 25 mM MgCl₂, 3 μl 0.1M DTT, 1.2 μl 500 μM dNTPs, 0.7 μl RNasin, and 5.6 μl DEPC-treated water. The tubes were incubated for 2 minutes at room temperature, after which 1.5 μl (300 units) Superscript II RNase H- reverse transcriptase (GIBCO) were added. The tubes were incubated sequentially at room temperature for 2 minutes, 60 minutes at 37°C, and 5 minutes at 95°C, followed by chilling on ice. PCR amplification was performed using a master mix containing 117 μl lOx PCR buffer (500 mM KC1, 100 mM Tris-HCl pH 8.3, 15 mM MgCl₂, and 0.01 % (w/v) gelatin), 70.2 μl 25 mM MgCl,, 5.9 μl alpha-"P dATP (10m Ci/ml, DuPont NEN), 4.7 μl 500 μM dNTP mix, 11 μl AmpliTaq DNA polymerase (5 units/μl, Perkin-Elmer), and 493.3 μl DEPC-treated water. For each reaction, 12 μl of the master mix was added to 2 μl downstream primer #7, 1 μl of cDNA, and 5 μl of upstream primer #15. The reaction mixes were heated to 94°C for 1 minute, then thermocycled 40 times with a denaturing step of 94°C for 15 seconds, annealing step of 40°C for 1 minute, and an extension step of 72"C for 30 _*1 7 seconds. Following the 40 cycles, the reactions were incubated at 72"C for 5 minutes and stored at 10°C. The PCR reactions were performed in a Perkin-Elmer GeneAmp System 9600 thermocycler.

Four microliters of the amplification reaction were mixed with an equal volume of loading buffer (0.2% bromphenol blue, 0.2% Xylene cyanol, 10 mM EDTA pH 8.0, and 20% glycerol). Four microliters of this mix was run on a 6% nondenaturing acrylamide sequencing format gel for 3 hours at 1200 volts (constant voltage). The gel was dried at 80°C for 1.5 hours and exposed to Kodak XAR film. An amplification product found only in the reaction containing cDNA from THP-1 cells exposed to oxidized LDL was identified and excised from the gel. 100 μl of DEPC-treated water was added to a microcentrifuge tube containing the excised gel fragment and was incubated for 30 minutes at room temperature followed by 15 minutes at 95°C.

To reamplify the PCR product, 26.5 microliters of the eluted DNA were used in a amplification reaction that also included 5 μl lOx PCR buffer, 3 μl 25 mM MgCl₂, 5 μl 500 μM dNTPs, 5 μl 2μM downstream primer 7, 7.5 μl upstream primer 15, and 0.5 μl Amplitaq polymerase. The PCR cycling parameters and instrument were as described above. Following amplification, 20 μl of the reamplification was analyzed on an agarose gel and 4 μl was used to subclone the PCR products into the vector pCRII using the TA cloning system (Frohman, M.A., Dush, M.K., and Martin, G.R. (1988) Proc. Natl. Acad. Sci. USA 85,8998-9002) (Invitrogen). Following an overnight ligation at 14°C, the ligation products were used to transform E. coli. Resulting transformants were picked and 3 ml overnight cultures were used in plasmid minipreparations. Insert sizes were determined using EcoRI digestions of the plasmids and clones containing inserts of the approximate size of the original PCR product were sequenced using fluorecent dye-terminator reagents (Prism, Applied Biosystems) and an Applied Biosystems 373 DNA sequencer. The sequence of the PCR product is shown in Figure 2. The sequence of the amplification primers is underlined.

C) 5 'RACE Reaction

Extension of the cDNA identified through RT-PCR was accomplished using the 5' RACE system (Loh, E.Y., Eliot, J.F., Cwirla, S., Lanier, L.L., and Davis, M.M. (1989) Science 243, 217- 219; Simms, D., Guan, N., and Sitaraman, K., (1991) Focus 13,99) (GIBCO). One microgram of the THP-1 RNA (oxidized LDL treated) used initially in the differential display reactions was utilized in the 5'RACE procedure: lμl (1 μg) of RNA was combined with 3 μl (3 pmol) primer 2a and 11 μl DEPC-treated water and heated to 70°C for 10 minutes followed by 1 minute on ice. 2.5 μl lOx reaction buffer (200 mM Tris-HCl pH 8.4, 500 mM KC1), 3 μl 25 mM MgCl₂, 1 μl 10 mM dNTP mix, and 2.5 μl 0.1 M DTT were added. The mix was incubated at 42°C for 2 minutes, then 1 μl Superscript II reverse transcriptase was added. The reaction was incubated for an additional 30 minutes at 42°C, 15 minutes at 70°C, and on ice for 1 minute. One microliter of RNase H (2 units) was added and the mixture was incubated at 55°C for 10 minutes. The cDNA was purified using the GlassMax columns (Sambrook, J. Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, second edition, Cold Spring Harbor Laboratory

Press, Plainview, NY) included in the kit. The cDNA was eluted from the column in 50 μl dH₂0, lyophilized, and resuspended in 21 μl dH₂0. Tailing of the cDNA was accomplished in the following reaction: 7.5 μl dH₂0, 2.5 μl reaction buffer (200 mM Tris-HCl pH 8.4, 500 mM KC1), 1.5 μl 25 mM MgCl₂, 2.5 μl 2 mM dCTP, and 10 μl of the cDNA were incubated at 94°C for 3 minutes, then 1 minute on ice. 1 μl (10 units) of terminal deoxynucleotidyl transferase was added and the mixture was incubated for 10 minutes at 37°C. The enzyme was heat inactivated by incubation at 70°C for 10 minutes and the mixture was placed on ice. PCR amplification of the cDNA was performed in the following steps: 5 μl of the tailed cDNA was included in a reaction which also contained 5 μl lOx PCR buffer (500 mM KC1, 100 mM Tris- HC1 pH 8.3, 15 mM MgCl₂, and 0.01 % (w/v) gelatin), 1 μl 10 mM dNTP mix, 2 μl (10 pmol) anchor primer, 1 μl (20 pmol) primer 3a, and 35 μl dH₂0. The reaction was heated to 95°C for 1 minute, then 0.9 μl (4.5 units) Amplitaq polymerase was added. The reaction was cycled 40 times under the following conditions: 94°C for 5 seconds, 50"C for 20 seconds, and 72°C for 30 seconds. One microliter of this reaction was used in a nested reamplification to increase levels of specific product for subsequent isolation. The reamplification included: 1 μl primary amplification, 5 μl lOx PCR buffer, 1 μl 10 mM dNTP mix, 2 μl (20 pmol) universal amplification primer, 2 μl (20 pmol) primer 4a, and 38 μl dH₂0. The reaction was heated to 95°C for 1 minute, then 0.7 μl (3.5 units) Amplitaq polymerase was added. The reaction was cycled 40 times under these conditions; 94°C for 5 seconds, 50°C for 20 seconds, and 72°C for 30 seconds. The amplification products were analyzed via 0.8% agarose gel electrophoresis. A predominant product of approximately 1.2 kilobase pairs was detected. Two microliters of the reaction products were cloned into the pCRII vector from the TA cloning kit (Invitrogen) and incubated at 14°C overnight. The ligation products were used to transform E. coli. The insert sizes of the resulting transformants were determined following EcoRI digestion. Clones containing inserts of the approximate size of the PCR product were sequenced using fluorescent dye-terminator reagents (Prism, Applied Biosystems) and an Applied Biosystems 373 DNA sequencer. The sequence of the RACE product including the EcoRI sites from the TA vector are shown in Figure 3. The sequences of the amplimers (universal amplification primer and the complement to 5 'RACE primer 4a) are underlined. EXAMPLE 2

Cloning and Chromosomal Localization of the LLGXL Gene A) cDNA library screening

A human placental cDNA library (Oligo dT and random primed, Cat #5014b, Lot

#52033) was obtained from Clontech (Palo Alto, CA). A radiolabeled probe was created by excising the insert of a plasmid containing the 5'RACE reaction PCR product described above.

The probe was radiolabeled using the random priming technique: the DNA fragment (50-100 ng) was incubated with 1 μg of random hexamers (Gibco) at 95°C for 10 minutes followed by 1 minute on ice. At room temperature the following were added: 3 μl lOx Klenow buffer (100 mM Tris-HCl pH 7.5, 50 mM MgCL₂, 57 mM dithiothreitol; New England Biolabs), 3 μl 0.5 mM dATP, dGTP, dTTP), 100 μCi -¹²PdCTP (3000 Ci/mmol, New England Nuclear), and 1 μl Klenow fragment of DNA polymerase I (5 units, Gibco). The reaction was incubated for 2-3 hours at room temperature and the reaction was then stopped by increasing the volume to 100 μl with TE pH 8.0 and adding EDTA to a final concentration of 1 mM. The unincorporated nucleotides were removed by raising the reaction volume to 100 μl and passing over a G-50 spin column (Boehringer Mannheim). The resulting probes had a specific activity greater than 5xl0⁸ cpm/μg DNA. The library was probed using established methods (Walter, P., Gilmore, R., and Blobel, G.

(1984) Cell 38,5-8). Briefly, the filters were hybridized for 24 hours at 65°C in 4.8X SSPE (20X SSPE = 3.6 M NaCl, 0.2 M NaH₂P0₄, 0.02 M EDTA, pH 7.7), 20 mM Tris-HCl pH 7.6, IX Denhardt's solution (100X= 2% Ficoll 400, 2% polyvinylpyrrolidone, 2% BSA), 10% dextran sulfate, 0.1 % SDS, 100 μg/ml salmon sperm DNA, and l xl0⁶cpm/ml radiolabelled probe. Filters were then washed three times for 15 minutes at room temperature in 2X SSC (IX SSC = 150 mM NaCl, 15 mM sodium citrate pH 7.0), 0.1 % sodium dodecyl sulfate (SDS) followed by three washes for 15 minutes each at 65°C in 0.5X SSC, 0.1% SDS. Phage which hybridized to the probe were isolated and amplified. DNA was purified from the amplified phage using LambdaSorb reagent (Promega) according to the manufacturer's instructions. The inserts were excised from the phage DNA by digestion with EcoRI. The inserts were subcloned into the EcoRI site of a plasmid vector (Bluescript II SK, Stratagene). The sequence of the open reading frame contained within the 2.6 kb EcoRI fragment of the cDNA was determined by automated sequencing as described above. The sequence is shown in Figure 4. The amino acid sequence of the predicted protein encoded by the open reading frame is shown in Figure 5 and has been termed LLGXL. The first methionine is predicted to be encoded by nucleotide pairs 252-254. The predicted protein is 500 amino acids in length. The first 18 amino acids form a sequence characteristic of a secretory signal peptide (Higgins, D.G., and Sharp, P.M. (1988) Gene 73, 237-244). The propeptide is predicted to have a molecular weight of 56,800 Daltons. Assuming cleavage of the signal peptide at position 18, the unmodified mature protein has a molecular weight of 54,724 Daltons.

The overall similarities between this protein and the other known members of the triacylglycerol lipase family is illustrated in Figure 6 and Table 1. In the alignment shown in Figure 6, LLG is the polypeptide (SEQ ID NO: 6) encoded by the cDNA (SEQ ID NO: 5) described in Example 1, and hereafter referred to as LLGN. This protein is identical with the LLGXL protein in the amino terminal 345 residues. Nine unique residues are followed by a termination codon, producing a propolypeptide of 39.3 kD and a mature protein of 37.3 kD. The sequences which are common to LLGN and LLGXL are nucleic acid sequence SEQ ID NO: 9 and amino acid sequence SEQ ID NO: 10.

Interestingly, the position at which the LLGN and LLGXL proteins diverge is at a region known from the structure of the other lipase to be between the amino and carboxy domains of the proteins. Therefore, the LLGN protein appears to consist of only one of the two domains of triaclyglycerol lipases. This sequence contains the characteristic "GXSXG" lipase motif at positions 167-171 as well as conservation of the catalytic triad residues at Ser 169, Asp 193, and His 274. Conservation of cysteine residues (positions 64, 77, 252, 272, 297, 308, 311, 316, 463, and 483) which have been implicated in disulfide linkage in the other lipases suggests that the LLGXL protein has structural similarities to the other enzymes. T ere are five predicted sites for N-linked glycosylation; at amino acid positions 80, 136, 393, 469, and 491. The protein sequences used in the comparisons are human lipoprotein lipase (LPL; Genbank accession #M15856, SEQ ID NO: 13), Human hepatic lipase (HL; Genbank accession #J03540, SEQ ID NO: 14), human pancreatic lipase (PL; Genbank accession # M93285, SEQ ID NO: 15), human pancreatic lipase related protein- 1 (PLRP- 1 ; Genbank accession # M93283), and human pancreatic lipase related protein-2 (PLRP-2; Genbank accession # M93284).

TABLE 1. Similarity of triacylglycerol lipase gene family

Percent similarity was based on pairwise alignment using the Clustal algorithm (Camps, L., Reina, M., Llobera, M., Vilaro, S., and Olivecrona, T. (1990) Am. J. Physiol. 258,C673-C681) in the Megalign program of the Lasergene Biocomputing Software Suite (Dnastar).

B) Chromosomal Localization

DNA from a PI clone (Steinberg, N., Ruether, J. and DeRiel, K. The New Biologist 2: 151-62, 1990) containing genomic LLG DNA was labelled with digoxigenin UTP by nick translation. Labelled probe was combined with sheared human DNA and hybridized to PHA stimulated peripheral blood lymphocytes from a male donor in a solution containing 50% formamide, 10% dextran sulfate, and 2X SSC. Specific hybridization signals were detected by incubating the hybridized cells in fluoresceinated antidigoxigenin antibodies followed by counterstaining with DAPI. This initial experiment resulted in specific labeling of a group E chromosome, which was believed to be chromosome 18 on the basis of DAPI staining.

A second experiment was conducted in which a biotin labelled probe specific for the centromere of chromosome 18 was cohybridized with the LLG probe. This experiment resulted in the specific labeling of the chromosome 18 centromere in red and the long arm of chromosome 18 in green. Measurements of 11 specifically labelled hybridized chromosomes 18 demonstrated that LLG has a Flter of 0.67 (Franke measurement of 0.38), which corresponds to band 18q21. Several genetic diseases, including intrahepatic cholestasis, cone rod dystrophy, and familial expansile osteolysis, are believed to involve defects in this chromosomal region.

EXAMPLE 3 LLG RNA Analysis A) Expression of LLG RNA in THP-1 cells Analysis of the mRNA from which the cDNA was derived was performed by northern analysis of THP-1 RNA. RNA from these cells was prepared as described above. The mRNA was purified from the total RNA through the use of a poly-dT-magnetic bead system (Polyattract system, Promega). Three micrograms of poly (A)-containing mRNA was electrophoresed on a 1% agarose-formaldehyde gel. The gel was washed for 30 minutes in dH₂0. RNAs were vacuum transferred to a nylon membrane using alkaline transfer buffer (3M NaCl, 8 mM NaOH, 2 mM sarkosyl). After transfer, the blot was neutralized by incubation for 5 minutes in 200 mM phosphate buffer pH 6.8. The RNA was crosslinked to the membrane using an ultraviolet crosslinker apparatus (Stratagene).

A probe was made by excising the insert of a plasmid containing the 5'RACE reaction PCR product described above. The probe was radiolabeled using the random priming technique described in Example 2. The filters were prehybridized in QuikHyb rapid hybridization solution (Stratagene) for 30 minutes at 65°C. The radiolabeled probe (1-2 x 10⁶ cpm/ml) and sonicated salmon sperm DNA (final concentration 100 μg/ml) were denatured by heating to 95°C for 10 minutes and quick-chilled on ice before adding to the filter in QuikHyb. Hybridization was for 3 hours at 65°C. The unhybridized probe was removed by washing the filters two times for 15 minutes with 2X SSC, 0.1 % sodium dodecyl sulfate at room temperature followed by two times for 15 minutes in 0.1 X SSC, 0.1% SDS at 62°C. Following the washes, the filters were allowed to dry briefly and then exposed to Kodak XAR-2 film with intensifying screens at -80°C. The results are shown in Figure 7, which shows a major mRNA species of approximately 4.5 kilobases. Minor species of 4.3 and 1.6 kilobases are also present. The expected size of the LLGN cDNA is 1.6 kb. The LLGXL sequence is likely to be encoded by the major species of mRNA detected.

B) Expression of LLG RNA in various human tissues. A commercially prepared filter containing 3 μg each of mRNAs from human tissues

(heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas) was obtained from Clontech (Catalog #7760-1). This filter was probed and processed as described above. After probing with the radiolabeled LLG fragment and autoradiography, the probe was stripped by washing in boiling 0.1X SSC, 0.1 % SDS for 2 xl5 min. in a 65°C incubator. The membranes were then probed with a 1.4 kilobase pair DNA fragment encoding human lipoprotein lipase. This fragment was obtained by RT-PCR of the THP-1 RNA (PMA and oxLDL treated) using the 5 'LPL and 3 'LPL primers described in Figure 1. and the RT-PCR conditions described above. After autoradiography, the membranes were stripped again and reprobed with a radiolabeled fragment of the human beta actin cDNA to normalize for RNA content. The results of these analyses are shown in Figure 8. The highest levels of LLG message were detected in placental RNA, with lower levels found in RNAs derived from lung, liver, and kidney tissue. In agreement with previous studies by others (Verhoeven, A.J.M., Jansen, H. (1994) Biochem. Biophys. Acta 1211,121-124), lipoprotein lipase message was found in many tissues, with highest levels found in heart and skeletal muscle tissue. Results of this analysis indicates that the tissue distribution of LLG expression is very different from that of LPL. The pattern of LLG expression is also different from that of either hepatic lipase or pancreatic lipase, as reported by others (Wang, C.-S., and Hartsuck, J.A. (1993) Biochem. Biophys. Acta 1166,1-19; Semenkovich, C.F., Chen, S.-W., Wims, M., Luo C.-C, Li, W.-H., and Chan, L. (1989) J. Lipid Res. 30,423-431 ; Adams, M.D., Kerlavage, A.R., Fields, C, and Venter, C. (1993) Nature Genet. 4,256-265) .

To determine the expression pattern in additional human tissues, another commercially prepared membrane was probed with LLGXL cDNA. This dot blot (Human RNA Master Blot, Clontech Cat. # 7770-1) contains 100-500 ng mRNA from 50 different tissues and is normalized for equivalent housekeeping gene expression (Chen, L., and Morin, R. (1971) Biochim. Biophys. Acta 231,194-197). A 1.6 kb Dral-Srfl fragment of the LLGXL cDNA was labeled with ³²PdCTP using a random oligonucleotide priming system (Prime It II, Stratagene) according to the manufacturer's instructions. After 30 minutes prehybridization at 65°C, the probe was added to QuikHyb hybridization solution at 1.3xl0⁶cpm/ml. Hybridization was for 2 hours at 65°C . The unhybridized probe was removed by washing the filters two times for 15 minutes with 2X SSC, 0.1 % sodium dodecyl sulfate at room temperature followed by two times for 15 minutes in 0.1X SSC, 0.1% SDS at 62°C. Following the washes, the filters were allowed to dry briefly and then exposed to Kodak XAR-2 film with intensifying screens at -80°C. for varying amounts of time. The resulting images were quantitated by densitometry. The results are shown in Table 2. The relative expression levels of tissues represented in both the multiple tissue northern and the multiple tissue dot blot are similar, with highest levels in placenta, and lower levels in lung, liver and kidney. Fetal liver, kidney, and lung also express roughly the same levels as the adult tissues. Surprisingly, thyroid tissue expression levels were the highest of all tissues represented, with expression of 122% of that in placental tissue. While there is precedence for lipase expression by the placenta (Rothwell, J.E., Elphick, M.C. (1982) J. Dev. Physiol. 4,153-159; Verhoeven, A.J.M., Carling D., and Jansen H. (1994) J. Lipid Res. 35, 966- 975; Burton, B.K., Mueller, H.W. (1980) Biochim. Biophys. Acta 618,449-460), the thyroid was not previously known to express any lipase. These results suggest that LLG expression may be involved in maintenance of the placenta, where LLG may serve to liberate free fatty acids from substrates such as phospholipids as a source of energy. The LLG expressed in the thyroid may provide precursors for the synthesis of bioactive molecules by that gland.

Table 2. Expression of LLG mRNA in various human tissues

Values given are percentage of expression with levels in placental tissue arbitrarily set at 100%. Values are average of densitometric measurements from two autoradiographic exposures. N.D. = not detectable.

C) Expression of LLG RNA in cultured endothelial cells.

Human umbilical vein endothelial cells (HUVEC) and human coronary arterial endothelial cells (HCAEC) were obtained from Clonetics. HUVECs were propagated in a commercially prepared endothelial cell growth medium (EGM, Clonetics) supplemented with 3 mg/ml bovine brain extract (Maciag, T., Cerundolo, J., Ilsley, S., Kelley, P.R., and Forand, R. (1979) Proc. Natl. Acad. Sci. USA 76, 5674-5678), Clonetics), while HCAECs were propagated in EGM supplemented with 3 mg/ml bovine brain extract and 3% fetal bovine serum (5% final concentration). Cells were grown to confluence, then the medium was changed to EGM without bovine brain extract. Cultures were stimulated by adding 100 ng/ml of phorbol myristate (Sigma). After 24 hours incubation, the RNAs were extracted from the cells via the Trizol method described above. Twenty micrograms of total RNA was electrophoresed and transferred to the membrane for analysis. The membranes were probed with LLG and LPL probes as described above. The results are shown in Figure 9. Twenty micrograms of total RNA from THP-1 cells stimulated with PMA was run on the blot for comparison. RNA hybridizing to the LLG probe was detected in unstimulated and PMA stimulated HUVEC cells. In contrast, detectable levels of LLG mRNA were only found in HCAEC cultures after stimulation with PMA. In agreement with previous studies of others, no detectable lipoprotein lipase mRNA was detected in any of the endothelial RNAs (Verhoeven, A.J.M., Jansen, H. (1994) Biochem. Biophys. Acta 1211,121-124). EXAMPLE 4 LLG Protein Analysis A) Antibody preparation.

Antisera were generated to peptides with sequences corresponding to a region of the predicted protein encoded by the LLG cDNA open reading frame. This peptide was chosen because of its high predicted antigenicity index (Jameson B.A., and Wolf, H. (1988) Comput. Applic. in the Biosciences 4,181-186). The sequence of the immunizing peptide was not found in any protein or translated DNA sequence in the Genbank database. Its corresponding position in the LLG protein is shown in Figure 10. The carboxy terminal cysteine of the peptide does not correspond to the residue in the LLG putative protein, but was introduced to facilitate coupling to the carrier protein. The peptide was synthesized on a Applied Biosystems Model 433A peptide synthesizer. Two milligrams of peptide was coupled to two milligrams of maleimide-activated keyhole limpet hemocyanin following the protocols included in the Imject Activated Immunogen Conjugation Kit (Pierce Chemical). After desalting, one-half of the conjugate was emulsified with an equal volume of Freund's complete adjuvant (Pierce). This emulsification was injected into a New Zealand White rabbit. Four weeks after the initial inoculation, a booster inoculation was made with an emulsification made exactly as described above except Freund's incomplete adjuvant (Pierce) was used. Two weeks after the boost, a test bleed was made and titers of specific antibodies were determined via ELISA using immobilized peptide. A subsequent boost was made one month after the first boost.

B) Western analysis of medium from endothelial cell cultures HUVEC and HCEAC cells were cultured and stimulated with PMA as described in

Example 3C, except that the cells were stimulated with PMA for 48 hours. Samples of conditioned medium (9 ml) were incubated with 500 μl of a 50% slurry of heparin-Sepharose CL-6B in phosphate buffered saline (PBS, 150 mM sodium chloride, 100 mM sodium phosphate, pH 7.2). Heparin-Sepharose was chosen to partially purify and concentrate the LLG proteins because of the conservation of residues in the LLGXL sequence which have been identified as critical for the heparin-binding activity of LPL (Ma, Y., Henderson, H.E., Liu, M.- S., Zhang, H., Forsythe, I.J., Clarke-Lewis, I., Hayden, M.R., and Brunzell, J.D. J. Lipid Res. 35, 2049-2059; and Fig. 6.). After rotation at 4°C for 1 hour, the samples were centrifuged for 5 minutes at 150 x g. The medium was aspirated and the Sepharose was washed with 14 ml PBS. After centrifugation and aspiration, the pelleted heparin-Sepharose was suspended in 200 μl 2x SDS loading buffer (4% SDS, 20% glycerol, 2% β-mercaptoethanol, 0.002% bromphenol blue, and 120 mM Tris pH 6.8). The samples were heated to 95°C for 5 minutes and 40 μl was loaded onto a 10% Tris-Glycine SDS gel. After electrophoresis at 140 V for approximately 90 minutes, the proteins were transferred to nitrocellulose membranes via a Novex electroblotting apparatus (210 V, 1 hour). The membranes were blocked for 30 minutes in blocking buffer (5% nonfat dried milk, 0.1% Tween 20, 150 mM sodium chloride, 25 mM Tris pH 7.2). Antipeptide antisera and normal rabbit serum was diluted 1 :5000 in blocking buffer and was incubated with the membranes overnight at 4^UC with gentle agitation. The membranes were then washed 4x 15 minutes with TBST (0.1 % Tween 20, 150 mM sodium chloride, 25 mM Tris pH 7.2). Goat anti-rabbit peroxidase conjugated antisera (Boehringer Mannheim) was diluted 1 :5000 in blocking buffer and incubated with the membrane for 1 hour with agitation. The membranes were washed as above, reacted with Renaissance chemiluminescent reagent (DuPont NEN),and exposed to Kodak XAR-2 film. The results are shown in Figure 1 1. Two species of immunoreactive proteins are present in the samples from unstimulated HUVEC and HCAEC cells. Levels of immunoreactive protein in the unstimulated HCAEC samples are much lower than the corresponding HUVEC sample. Upon stimulation with PMA, three immunoreactive proteins are secreted by the endothelial cell cultures. PMA exposure greatly increased the level of LLG proteins produced by the HCAEC cultures. PMA induction of LLG proteins was not as dramatic in the HUVEC cultures.

EXAMPLE 5 Recombinant LLG Protein Production

A) LLG expression constructs

The cDNAs encoding the LLGN and LLGXL proteins were cloned into the mammalian expression vector pCDNA3 ( Invitrogen). This vector allows expression of foreign genes in many mammalian cells through the use of the cytomegalovirus major late promoter. The LLGN 5'RACE product was cloned into the EcoRI site of pCDNA3. The LLGXL cDNA was digested with Dral and Srfl to yield a 1.55 kb cDNA (see Figure 4.). The vector was digested with the restriction enzyme EcoRV and the vector and insert were ligated using T4 DNA ligase and reagents from the Rapid Ligation Kit (Boehringer Mannheim) according to the manufacturers instructions. The ligation products were used to transform competent E coli . Resultant colonies were screened by restriction analysis and sequencing for the presence and orientation of the insert in the expression vector.

B) Transient transfection of LLG in COS-7 cells.

The LLG expression vectors were introduced into COS-7 cells through the use of Lipofectamine cationic lipid reagent (GIBCO). Twenty-four hours before the transfection,

COS-7 cells were plated onto 60 mm tissue culture dishes at a density of 2xl0⁵ cells/plate. The cells were propagated in Dulbecco's modified Eagle's medium (DMEM; GIBCO) ^7 supplemented with 10% fetal calf serum, 100 U/ml penicillin, 100 μg/ml streptomycin. One microgram of plasmid DNA was added to 300 μl of Optimem I serum-free medium (Gibco). Ten microliters of Lipofectamine reagent were diluted into 300 μl of Optimem I medium and this was combined with the DNA solution and allowed to sit at room temperature for 30 minutes. The medium was removed from the plates and the cells were rinsed with 2 ml of

Optimem medium. The DNA-Lipofectamine solution was added to the plates along with 2.7 ml Optimem medium and the plates were incubated for 5 hours at 37"C. After the incubation, the serum free medium was removed and replaced with DMEM supplemented with 2% FBS and antibiotics. Twelve hours post-transfection, some of the cultures were treated with either 0.25 mM Pefabloc SC (Boehringer Mannheim), a protease inhibitor, or 10 U/ml heparin. Thirty minutes before harvest, the heparin treated samples were treated with an additional 40 U/ml heparin. The medium was removed from the cells 60 hours after transfection. Heparin- Sepharose CL-4B (200 μl of a 50% slurry in PBS pH 7.2) was added to 1 ml of medium and was mixed at 4"C for 1 hour. The Sepharose was pelleted by low speed centrifugation and was washed three times with 1 ml cold PBS. The Sepharose was pelleted and suspended in 100 μl 2x loading buffer. The samples were heated to 95°C for 5 minutes. 40 μl of each sample was loaded onto a 10% SDS-PAGE gel. Electrophoresis and western analysis was performed using the anti-LLG antiserum as described above. The results are shown in Figure 12. Proteins from HCAEC conditioned medium were included for size references. LLGN migrates at approximately 40 kD, corresponding to the lowest band in HCAEC. The medium from COS cells transfected with LLGXL cDNA contains both 68 kD and 40 kD species. When these cells were treated with heparin, the amount of both 68 kD and 40 kD proteins recovered from the medium increased dramatically, indicating either the release of proteoglycan-bound protein from the cell surface or stabilization of the proteins by heparin. When the cells were treated with the protease inhibitor Pefabloc, the amount of 68 kD protein increased relative to that of the 40 kD species. This suggests that the lower molecular weight protein produced by these cells is a proteolysis product of the larger 68 kD form. The role of the mRNA identified through differential display which encodes a shorter, 40 kD species is not known. There has, however, been a report of an alternately-spliced form of hepatic lipase which apparently is expressed in a tissue-specific manner and would create a truncated protein.

EXAMPLE 6 LLG in Animal Species

A) Cloning the Rabbit Homolog of LLG A commercially available lambda cDNA library derived from rabbit lung tissue

(Clontech, Cat. #TL1010b) was used to isolate a fragment of the rabbit homolog of the LLG gene. Five microliters of the stock library were added to 45 μl water and heated to 95°C for 10 minutes. The following were added in a final volume of 100 μl: 200 μM dNTPs, 20 mM Tris- HCl pH 8.4, 50 mM KC1, 1.5 mM MgCl₂, 100 μM each primer DLLP774 and LLGgen2a, and 2.5 U Taq polymerase (GIBCO). The reaction was thermocycled 35 times with the parameters of: 15 seconds at 94°C, 20 seconds at 50°C and 30 seconds at 72°C. Ten microliters of the reaction was analyzed via agarose gel electrophoresis. A product of approximately 300 basepairs was detected. A portion (4 μl) of the reaction mix was used to clone the product via the TA cloning system. The insert of a resulting clone was sequenced (SEQ ID NO: 1 1). An alignment between the deduced rabbit amino acid sequence (SEQ ID NO: 12) and the corresponding sequence of the human cDNA is also shown in Figure 14. Of the nucleotides not part of either amplification primer, there is an 85.8% identity between the rabbit and human LLG sequences. The predicted protein encoded by this rabbit cDNA shares 94.6 % identity with that of the human protein, with most of the nucleotide substitutions in the third or "wobble" positions of the codons. Notably, this region spans the "lid" sequence of the predicted LLG proteins and is a variable domain in the lipase gene family. This is evidence that there is a high degree of conservation of this gene between species.

B) LLG in Other Species

To demonstrate the presence of LLG genes in other species, genomic DNAs from various species were restriction digested with EcoRI, separated by electrophoresis in agarose gels, and blotted onto nitrocellulose membranes.

The membranes were hybridized overnight at 65°C with 2.5 x 10⁶ cpm/ml of random primed ³² P-LLG or ³²P-LPL (lipoprotein lipase) probe in a hybridization solution of 6X SSC, 10% dextran sulfate, 5 X Dendardt's solution, 1 % SDS, and 5 μg/ml salmon sperm DNA. The membranes were washed with 0.1X SSC, 0.5% SDS for ten minutes at room temperature, then sequentially for ten minutes at 40°C, 50°C, and 55°C. Autoradiograms of the blots are shown in Figure 16.

Figure 16 shows the presence of LLG and LPL genes in all species examined, with the exception that no hybridization was observed with the LLG probe against rat DNA. The exceptional data from rat may represent an artifact caused by generation of abnormally sized restriction fragments containing LLG sequences. Such fragments may be outside of the fractionation range of the agarose gel or may blot inefficiently. The different bands detected by the two probes indicate that LPL and LLG are separate, evolutionarily conserved genes.

EXAMPLE 7 ENZYMATIC ACTIVITY OF LLGXL

A) Phospholipase Activity Conditioned media from COS-7 cells transiently expressing human lipoprotein lipase (LPL), LLGN, or LLGXL were assayed for phospholipase activity. MEM containing 10% FBS (MEM) was used as the blank, and conditioned media from COS-7 cells transfected with an antisense LLGXL plasmid (AS) was used as a negative control. A phosphatidylcholine (PC) emulsion was made up using 10 μl phosphatidylcholine (10 mM), 40 μl ^l C-phosphatidylcholine, dipalmitoyl (2 μCi), labelled at the sn 1 and 2 positions, and 100 μl Tris-TCNB [100 mM Tris, 1 % Triton, 5 mM CaCl₂, 200 M NaCl, 0.1 % BSA). The emulsion was evaporated for 10 minutes, then brought to a final volume of 1 ml in Tris-TCNB. Reactions were performed in duplicate and contained 50 μl PC emulsion and 950 μl medium. Samples were incubated in a shaking water bath for 2-4 hours at 37°C. The reactions were terminated by adding 1 ml IN HC1, then extracted with 4 ml of 2-propanol:hexane (1 :1). The upper 1.8 ml hexane layer was passed through a silica gel column, and the liberated ^l4C-free fatty acids contained in the flow-thru fraction were quantitated in a scintillation counter. The results of these assays are shown in Figure 14.

B) Triacylglycerol Lipase Activity

Conditioned media from COS-7 cells transiently expressing human lipoprotein lipase (LPL), LLGN, or LLGXL were assayed for triglycerol lipase activity. MEM containing 10% FBS was used as the blank, and conditioned media from COS-7 cells transfected with an antisense LLGXL plasmid (AS) was used as a negative control.

A concentrated substrate was prepared as an anhydrous emulsion of labeled triolein, [9,10- ³H(N)] and unlabeled triolein (final total triolein = 150 mg with 6.25 x 10⁸cpm), which was stabilized by adding 9 mg of lecithin in 100% glycerol. 0.56 ml of ³H-triolein, (0.28 mCi) was mixed with 0.17 ml of unlableled triolein and 90 μl of lecithin (9 mg). The mixture was evaporated under a stream of nitrogen. The dried lipid mixture was emulsified in 2.5 ml 100% glycerol by sonication (30 second pulse level 2 followed by 2 second chill cycles over 5 minutes].

The assay substrate was prepared by dilution of 1 volume of concentrated substrate with 4 volumes of 0.2M Tris-HCl buffer (pH 8.0) containing 3% w/v fatty acid free bovine serum albumin. The diluted substrate was vortexed vigorously for 5 seconds.

Reactions were performed in duplicate in a total volume of 0.2 ml containing 0.1 ml of assay substrate and 0.1 ml of the indicated conditioned media. The reactions were incubated for 90 minutes at 37°C. The reactions were terminated by adding 3.25 ml of methanol-chloroform- heptane 1.41: 1.25: 1 (v/v/v) followed by 1.05 ml of 0.1M potassium carbonate-borate buffer (pH 10.5). After vigorous mixing for 15 seconds, the samples were centrifuged for 5 minutes at

1000 rpm. A 1.0 ml aliquot of the upper aqueous phase was counted in a scintillation counter. The results of these assays are shown in Figure 15. EXAMPLE 8

USE OF LLG POLYPEPTIDE TO SCREEN FOR AGONISTS OR ANTAGONISTS Recombinant LLG is produced in baculovirus-infected insect cells. Recombinant LLG is purified from the serum-containing or serum-free conditioned medium by chromatography on heparin-Sepharose, followed by chromatography on a cation exchange resin. A third chromatographic step, such as molecular sieving, is used in the purification of LLG if needed.

During purification, anti-peptide antibodies are used to monitor LLG protein and the phospholipase assay is used to follow LLG activity.

In the fluorescent assay, the final assay conditions are approximately 10 mM Tris-HCl (pH 7.4), 100 mM KC1, 2 mM CaCl_2, 5 μM C₆NBD-PC{ l-acyl-2-[6-(nitro-2,l,3-benzoxadiazol- 4-yl)amino] caproylphosphatidylcholine, and LLG protein (approx 1-100 ng). The reaction is subjected to fluorescence excitation at 470 nm, and enzyme activity, as measured by the fluorescence emission at 540 nm is continuously monitored. Compounds and/or substances to be tested for stimulation and/or inhibition of LLG activity are added as 10-200 mM solutions in dimethylsulfoxide. Compounds which stimulate or inhibit LLG activity are identified as causing an increased or decreased fluorescence emission at 540 nm.

In the thio assay, the final assay conditions are approximately 25 mM Tris-HCl (pH 8.5), 100 mM KC1, 10 mM CaCl₂, 4.24 mM Triton X-100, 0.5 mM l,2-bis(hexanoylthio)-l,2- dideoxy-sn-glycero-3-phosphorylcholine, 5 mM 4,4'-dithiobispyridine (from a 50 mM stock solution in ethanol), and 1-100 ng recombinant LLG. Phospholipase activity is determined by measuring the increase in absorption at 342 nm. Compounds and/or substances to be tested for stimulation and/or inhibition of LLG activity are added as 10-200 mM solutions in dimethylsulfoxide. Compounds which stimulate or inhibit LLG activity are identified as causing an increased or decreased absorption at 342 nm.

EXAMPLE 9 TRANSGENIC MICE EXPRESSING HUMAN LLG To further study the physiological role of LLG, transgenic mice expressing human LLG are generated.

The 1.53 kb Dral Srfl restriction fragment encoding LLGXL (see Figure 4) was cloned into a plasmid vector (pHMG) downstream of the promoter for the ubiquitously expressed 3- hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase gene. Transgenic mice expressing different levels of human LLGXL are generated using standard methods (see, e.g.,

G.L. Tromp et al. Gene 1565:199-205, 1995). The transgenic mice are used to determine the impact of LLGXL overexpression on lipid profile, vascular pathology, rate of development and severity of atherosclerosis, and other physiological parameters.

EXAMPLE 10 EXPRESSION OF LLG IN ATHEROSCLEROTIC TISSUES

LLGXL expression in atherosclerosis was examined by performing a reverse transcription- polymerase chain reaction (RT-PCR) using mRNA isolated from vascular biopsies from four patients with atherosclerosis. The tissue samples were from the aortic wall (one sample), the iliac artery (two samples), and the carotid artery (one sample). Atherosclerosis biopsies were received from Gloucestershire Royal Hospital, England, and polyA+ mRNA was prepared and resuspended in diethylpyrocarbonate (DEPC) treated water at a concentration of 0.5 μg/μl mRNA. Reverse transcriptase reactions were performed according to the GibcoBRL protocol for Superscript Preamplification System for First Strand cDNA Synthesis. Briefly, the cDNA was synthesized as follows: 2 μl of each mRNA was added to 1 μl oligo (dT)₁₂.₁₈ primer and 9 μl of DEPC water. The tubes were incubated at 70 °C for 10 minutes and put on ice for 1 minute. To each tube, the following components were added: 2 μl 10X PCR buffer, 2 μl 25 mM MgCl₂, 1 μl 10 M dNTP mix and 2 μl 0.1M DTT. After 5 minutes at 42 °C, 1 μl (200 units) of Super Script II reverse transcriptase was added. The reactions were mixed gently, then incubated at 42 °C for 50 minutes. The reactions were terminated by incubation at 70 "C for 15 minutes then put on ice. The remaining mRNA was destroyed by the addition of 1 μl of RNase H to each tube and incubated for 20 minutes at 37 °C.

PCR amplifications were performed using 2 μl of the cDNA reactions. To each tube the following were added: 5 μl 10X PCR buffer, 5 μl 2 mM dNTPs, 1 μl hllg-gspl primer (20 pmol/ml, see Figure 1), 1 μl hllg-gsp2a primer (20 pmol/ml, see Figure 1), 1.5 μl 50 mM MgCl₂, 0.5 μl Taq polymerase (5U/ml) and 34 μl water. After holding the reactions at 95°C for 2 minutes, thirty cycles of PCR were performed as follows: 15 seconds at 94^UC, 20 seconds at 52°C, and 30 seconds at 72°C. The finished reactions were held for 10 minutes at 72°C before analysis by agarose gel electrophoresis. The hllg-gsp primers are specific for LLG and yield an expected product of 300 bp. In a parallel PCR to show that the cDNA synthesis reactions had been successful, primers specific for the housekeeping gene, G3PDH (human glyceraldehyde 3- phosphate dehydrogenase) were used (1 μl each at 20 pmol/ml).

The G3PDH primers (see Figure 1) yielded the expected product of 983 bp in all four vascular biopsy samples. LLG expression was detected in three of the four samples, with no expression being detected in the carotid artery sample. All the references discussed herein are incorporated by reference.

One skilled in the art will readily appreciate the present invention is well adapted to carry out the objects and obtain the ends and advantages mentioned, as well as those inherent therein. The peptides, polynucleotides, methods, procedures and techniques described herein are presented as representative of the preferred embodiments, and intended to be exemplary and not intended as limitations on the scope of the present invention. Changes therein and other uses will occur to those of skill in the art which are encompassed within the spirit of the invention or defined by the scope of the appended claims.

SEQUENCE LISTING

( 1 ) GENERAL INFORMATION :

(i) APPLICANT: Jaye, Michael C. Doan, Kim-Anh T. Kra iec, John A. Lynch, Kevin J. Amin, Dilip V.

South, Victoria J.

(ii) TITLE OF INVENTION: LLG POLYPEPTIDES OF THE TRIACYLGLYCEROL LIPASE FAMILY, AND COMPOSITIONS AND METHODS FOR THEIR USE IN ENZYMATIC HYDROLYSIS, AND PROTEIN AND GENE THERAPIES

(iii) NUMBER OF SEQUENCES: 31

(iv) CORRESPONDENCE ADDRESS: (A) ADDRESSEE: Rhone-Poulenc Rorer Inc.

(B) STREET: 500 Arcola Rd. 3C43

(C) CITY: Collegeville

(D) STATE: PA

(E) COUNTRY: USA (F) ZIP: 19426

(v) COMPUTER READABLE FORM:

(A) MEDIUM TYPE: Floppy disk

(B) COMPUTER: IBM PC compatible (C) OPERATING SYSTEM: PC-DOS/MS-DOS

(D) SOFTWARE: Patentln Release #1.0, Version #1.30

(vi) CURRENT APPLICATION DATA:

(A) APPLICATION NUMBER: US (B) FILING DATE:

(C) CLASSIFICATION:

(viii) ATTORNEY/AGENT INFORMATION:

(A) NAME: Fehlner Ph.D., Paul F. (B) REGISTRATION NUMBER: 35,135

(C) REFERENCE/DOCKET NUMBER: A2582-WO

(ix) TELECOMMUNICATION INFORMATION: (A) TELEPHONE: (610)454-3839 (B) TELEFAX: (610)454-3808

(2) INFORMATION FOR SEQ ID NO : 1 : (i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 367 base pairs

(B) TYPE: nucleic acid ^_*^^

(C) STRANDEDNESS : double

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: cDNA

(ix) FEATURE:

(A) NAME/KEY: CDS

(B) LOCATION: 22..180

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:l:

GAATTCGGCT TGATCAATCG C TTC AAA AAG GGG ATC TGT CTG AGC TGC CGC 51 Phe Lys Lys Gly lie Cys Leu Ser Cys Arg

1 5 10

AAG AAC CGT TGT AAT AGC ATT GGC TAC AAT GCC AAG AAA ATG AGG AAC 99 Lys Asn Arg Cys Asn Ser lie Gly Tyr Asn Ala Lys Lys Met Arg Asn 15 20 25

AAG AGG AAC AGC AAA ATG TAC CTA AAA ACC CGG GCA GGC ATG CCT TTC 147 Lys Arg Asn Ser Lys Met Tyr Leu Lys Thr Arg Ala Gly Met Pro Phe 30 35 40

AGA GGT AAC CTT CAG TCC CTG GAG TGT CCC TGA GGAAGGCCCT TAATACCTCC 200 Arg Gly Asn Leu Gin Ser Leu Glu Cys Pro * 45 50 TTCTTAATAC CATGCTGCAG AGCAGGGCAC ATCCTAGCCC AGGAGAAGTG GCCAGCACAA 260

TCCAATCAAA TCGTTGCAAA TCAGATTACA CTGTGCATGT CCTAGGAAAG GGAATCTTTA 320

CAAAATAAAC AGTGTGGACC CCTCAAAAAA AAAAAAAAGC CGAATTC 367

(2) INFORMATION FOR SEQ ID NO: 2:

(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 53 amino acids

(B) TYPE: amino acid (D) TOPOLOGY: linear

(ii) MOLECULE TYPE: protein

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:2:

Phe Lys Lys Gly lie Cys Leu Ser Cys Arg Lys Asn Arg Cys Asn Ser 1 5 10 15 lie Gly Tyr Asn Ala Lys Lys Met Arg Asn Lys Arg Asn Ser Lys Met 20 25 30 Tyr Leu Lys Thr Arg Ala Gly Met Pro Phe Arg Gly Asn Leu Gin Ser 35 40 45 Leu Glu Cys Pro * 50

( 2 ) INFORMATION FOR SEQ ID NO : 3 : (i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1382 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS : double

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: cDNA

(ix) EATURE :

(A) NAME/KEY: CDS

(B) LOCATION: 312 1370

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:3:

GAATTCGGCT TCTACTACTA CTAGGCCACG CGTCGCCTAG TACGGGGGGG GGGGGGGGGG 60

TCAGCGAGTC CTTGCCTCCC GGCGGCTCAG GACGAGGGCA GATCTCGTTC TGGGGCAAGC 120 CGTTGACACT CGCTCCCTGC CACCGCCCGG GCTCCGTGCC GCCAAGTTTT CATTTTCCAC 180

CTTCTCTGCC TCCAGTCCCC CAGCCCCTGG CCGAGAGAAG GGTCTTACCG GCCGGGATTG 240

CTGGAAACAC CAAGAGGTGG TTTTTGTTTT TTAAAACTTC TGTTTCTTGG GAGGGGGTGT 300

GGCGGGGCAG G ATG AGC AAC TCC GTT CCT CTG CTC TGT TTC TGG AGC CTC 350 Met Ser Asn Ser Val Pro Leu Leu Cys Phe Trp Ser Leu 55 60 65 TGC TAT TGC TTT GCT GCG GGG AGC CCC GTA CCT TTT GGT CCA GAG GGA 398 Cys Tyr Cys Phe Ala Ala Gly Ser Pro Val Pro Phe Gly Pro Glu Gly 70 75 80

CGG CTG GAA GAT AAG CTC CAC AAA CCC AAA GCT ACA CAG ACT GAG GTC 446 Arg Leu Glu Asp Lys Leu His Lys Pro Lys Ala Thr Gin Thr Glu Val 85 90 95

AAA CCA TCT GTG AGG TTT AAC CTC CGC ACC TCC AAG GAC CCA GAG CAT 494 Lys Pro Ser Val Arg Phe Asn Leu Arg Thr Ser Lys Asp Pro Glu His 100 105 110

GAA GGA TGC TAC CTC TCC GTC GGC CAC AGC CAG CCC TTA GAA GAC TGC 542 ≠

Glu Gly Cys .Tyr Leu Ser Val Gly His Ser Gin Pro Leu Glu Asp Cys 115 120 125 130

AGT TTC AAC ATG ACA GCT AAA ACC TTT TTC ATC ATT CAC GGA TGG ACG 590 Ser Phe Asn Met Thr Ala Lys Thr Phe Phe lie lie His Gly Trp Thr

135 140 145

ATG AGC GGT ATC TTT GAA AAC TGG CTG CAC AAA CTC GTG TCA GCC CTG 638

Met Ser Gly lie Phe Glu Asn Trp Leu His Lys Leu Val Ser Ala Leu 150 155 160

CAC ACA AGA GAG AAA GAC GCC AAT GTA GTT GTG GTT GAC TGG CTC CCC 686

His Thr Arg Glu Lys Asp Ala Asn Val Val Val Val Asp Trp Leu Pro

165 170 175

CTG GCC CAC CAG CTT TAC ACG GAT GCG GTC AAT AAT ACC AGG GTG GTG 734

Leu Ala His Gin Leu Tyr Thr Asp Ala Val Asn Asn Thr Arg Val Val 180 185 190 GGA CAC AGC ATT GCC AGG ATG CTC GAC TGG CTG CAG GAG AAG GAC GAT 782

Gly His Ser lie Ala Arg Met Leu Asp Trp Leu Gin Glu Lys Asp Asp 195 200 205 210

TTT TCT CTC GGG AAT GTC CAC TTG ATC GGC TAC AGC CTC GGA GCG CAC 830 Phe Ser Leu Gly Asn Val His Leu lie Gly Tyr Ser Leu Gly Ala His

215 220 225

GTG GCC GGG TAT GCA GGC AAC TTC GTG AAA GGA ACG GTG GGC CGA ATC 878

Val Ala Gly Tyr Ala Gly Asn Phe Val Lys Gly Thr Val Gly Arg lie 230 235 240

ACA GGT TTG GAT CCT GCC GGG CCC ATG TTT GAA GGG GCC GAC ATC CAC 926

Thr Gly Leu Asp Pro Ala Gly Pro Met Phe Glu Gly Ala Asp lie His

245 250 255

AAG AGG CTC TCT CCG GAC GAT GCA GAT TTT GTG GAT GTC CTC CAC ACC 974

Lys Arg Leu Ser Pro Asp Asp Ala Asp Phe Val Asp Val Leu His Thr 260 265 270 TAC ACG CGT TCC TTC GGC TTG AGC ATT GGT ATT CAG ATG CCT GTG GGC 1022

Tyr Thr Arg Ser Phe Gly Leu Ser lie Gly He Gin Met Pro Val Gly 275 280 285 290

CAC ATT GAC ATC TAC CCC AAT GGG GGT GAC TTC CAG CCA GGC TGT GGA 1070 His He Asp He Tyr Pro Asn Gly Gly Asp Phe Gin Pro Gly Cys Gly

295 300 305

CTC AAC GAT GTC TTG GGA TCA ATT GCA TAT GGA ACA ATC ACA GAG GTG 1118

Leu Asn Asp Val Leu Gly Ser He Ala Tyr Gly Thr He Thr Glu Val 310 315 320

GTA AAA TGT GAG CAT GAG CGA GCC GTC CAC CTC TTT GTT GAC TCT CTG 1166 Val Lys Cys Glu His Glu Arg Ala Val His Leu Phe Val Asp Ser Leu 325 330 335

GTG AAT CAG GAC AAG CCG AGT TTT GCC TTC CAG TGC ACT GAC TCC AAT 1214 Val Asn Gin Asp Lys Pro Ser Phe Ala Phe Gin Cys Thr Asp Ser Asn 340 345 350

CGC TTC AAA AAG GGG ATC TGT CTG AGC TGC CGC AAG AAC CGT TGT AAT 1262 Arg Phe Lys Lys Gly He Cys Leu Ser Cys Arg Lys Asn Arg Cys Asn 355 360 365 370

AGC ATT GGC TAC AAT GCC AAG AAA ATG AGG AAC AAG AGG AAC AGC AAA 1310 Ser He Gly Tyr Asn Ala Lys Lys Met Arg Asn Lys Arg Asn Ser Lys 375 380 385

ATG TAC CTA AAA ACC CGG GCA GGC ATG CCT TTC AGA GGT AAC CTT CAG 1358 Met Tyr Leu Lys Thr Arg Ala Gly Met Pro Phe Arg Gly Asn Leu Gin 390 395 400 TCC CTG GAG TGT CAAGCCGAAT TC 1382

Ser Leu Glu Cys 405

(2) INFORMATION FOR SEQ ID NO: 4:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 353 amino acids

(B) TYPE: amino acid (D) TOPOLOGY: linear

(ii) MOLECULE TYPE: protein

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 4:

Met Ser Asn Ser Val Pro Leu Leu Cys Phe Trp Ser Leu Cys Tyr Cys 1 5 10 15

Phe Ala Ala Gly Ser Pro Val Pro Phe Gly Pro Glu Gly Arg Leu Glu 20 25 30

Asp Lys Leu His Lys Pro Lys Ala Thr Gin Thr Glu Val Lys Pro Ser 35 40 45 Val Arg Phe Asn Leu Arg Thr Ser Lys Asp Pro Glu His Glu Gly Cys 50 55 60

Tyr Leu Ser Val Gly His Ser Gin Pro Leu Glu Asp Cys Ser Phe Asn 65 70 75 80

Met Thr Ala Lys Thr Phe Phe He He His Gly Trp Thr Met Ser Gly 85 90 95 *fr

He Phe Glu Asn Trp Leu His Lys Leu Val Ser Ala Leu His Thr Arg 100 105 110 Glu Lys Asp Ala Asn Val Val Val Val Asp Trp Leu Pro Leu Ala His 115 120 125

Gin Leu Tyr Thr Asp Ala Val Asn Asn Thr Arg Val Val Gly His Ser 130 135 140

He Ala Arg Met Leu Asp Trp Leu Gin Glu Lys Asp Asp Phe Ser Leu 145 150 155 160

Gly Asn Val His Leu He Gly Tyr Ser Leu Gly Ala His Val Ala Gly 165 170 175

Tyr Ala Gly Asn Phe Val Lys Gly Thr Val Gly Arg He Thr Gly Leu 180 185 190 Asp Pro Ala Gly Pro Met Phe Glu Gly Ala Asp He His Lys Arg Leu 195 200 205

Ser Pro Asp Asp Ala Asp Phe Val Asp Val Leu His Thr Tyr Thr Arg 210 215 220

Ser Phe Gly Leu Ser He Gly He Gin Met Pro Val Gly His He Asp 225 230 235 240

He Tyr Pro Asn Gly Gly Asp Phe Gin Pro Gly Cys Gly Leu Asn Asp 245 250 255

Val Leu Gly Ser He Ala Tyr Gly Thr He Thr Glu Val Val Lys Cys 260 265 270 Glu His Glu Arg Ala Val His Leu Phe Val Asp Ser Leu Val Asn Gin 275 280 285

Asp Lys Pro Ser Phe Ala Phe Gin Cys Thr Asp Ser Asn Arg Phe Lys 290 295 300

Lys Gly He Cys Leu Ser Cys Arg Lys Asn Arg Cys Asn Ser He Gly 305 310 315 320

Tyr Asn Ala Lys Lys Met Arg Asn Lys Arg Asn Ser Lys Met Tyr Leu 325 330 335

Lys Thr Arg Ala Gly Met Pro Phe Arg Gly Asn Leu Gin Ser Leu Glu 340 345 350

Cys (2) INFORMATION FOR SEQ ID NO : 5 :

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1065 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS : double

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: cDNA

(ix) FEATURE:

(A) NAME/KEY: CDS

(B) LOCATION: 1..1065

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 5:

ATG AGC AAC TCC GTT CCT CTG CTC TGT TTC TGG AGC CTC TGC TAT TGC 48 Met Ser Asn Ser Val Pro Leu Leu Cys Phe Trp Ser Leu Cys Tyr Cys 355 360 365

TTT GCT GCG GGG AGC CCC GTA CCT TTT GGT CCA GAG GGA CGG CTG GAA 96

Phe Ala Ala Gly Ser Pro Val Pro Phe Gly Pro Glu Gly Arg Leu Glu 370 375 380 385

GAT AAG CTC CAC AAA CCC AAA GCT ACA CAG ACT GAG GTC AAA CCA TCT 144

Asp Lys Leu His Lys Pro Lys Ala Thr Gin Thr Glu Val Lys Pro Ser

390 395 400

GTG AGG TTT AAC CTC CGC ACC TCC AAG GAC CCA GAG CAT GAA GGA TGC 192

Val Arg Phe Asn Leu Arg Thr Ser Lys Asp Pro Glu His Glu Gly Cys 405 410 415 TAC CTC TCC GTC GGC CAC AGC CAG CCC TTA GAA GAC TGC AGT TTC AAC 240

Tyr Leu Ser Val Gly His Ser Gin Pro Leu Glu Asp Cys Ser Phe Asn 420 425 430

ATG ACA GCT AAA ACC TTT TTC ATC ATT CAC GGA TGG ACG ATG AGC GGT 288 Met Thr Ala Lys Thr Phe Phe He He His Gly Trp Thr Met Ser Gly 435 440 445

ATC TTT GAA AAC TGG CTG CAC AAA CTC GTG TCA GCC CTG CAC ACA AGA 336

He Phe Glu Asn Trp Leu His Lys Leu Val Ser Ala Leu His Thr Arg 450 455 460 465

GAG AAA GAC GCC AAT GTA GTT GTG GTT GAC TGG CTC CCC CTG GCC CAC 384

Glu Lys Asp Ala Asn Val Val Val Val Asp Trp Leu Pro Leu Ala His

470 475 480

CAG CTT TAC ACG GAT GCG GTC AAT AAT ACC AGG GTG GTG GGA CAC AGC 432 Gin Leu Tyr Thr Asp Ala Val Asn Asn Thr Arg Val Val Gly His Ser .485 490 495

ATT GCC AGG ATG CTC GAC TGG CTG CAG GAG AAG GAC GAT TTT TCT CTC 480 He Ala Arg Met Leu Asp Trp Leu Gin Glu Lys Asp Asp Phe Ser Leu 500 505 510

GGG AAT GTC CAC TTG ATC GGC TAC AGC CTC GGA GCG CAC GTG GCC GGG 528 Gly Asn Val His Leu He Gly Tyr Ser Leu Gly Ala His Val Ala Gly 515 520 525

TAT GCA GGC AAC TTC GTG AAA GGA ACG GTG GGC CGA ATC ACA GGT TTG 576 Tyr Ala Gly Asn Phe Val Lys Gly Thr Val Gly Arg He Thr Gly Leu 530 535 540 545 GAT CCT GCC GGG CCC ATG TTT GAA GGG GCC GAC ATC CAC AAG AGG CTC 624 Asp Pro Ala Gly Pro Met Phe Glu Gly Ala Asp He His Lys Arg Leu 550 555 560

TCT CCG GAC GAT GCA GAT TTT GTG GAT GTC CTC CAC ACC TAC ACG CGT 672 Ser Pro Asp Asp Ala Asp Phe Val Asp Val Leu His Thr Tyr Thr Arg 565 570 575

TCC TTC GGC TTG AGC ATT GGT ATT CAG ATG CCT GTG GGC CAC ATT GAC 720 Ser Phe Gly Leu Ser He Gly He Gin Met Pro Val Gly His He Asp 580 585 590

ATC TAC CCC AAT GGG GGT GAC TTC CAG CCA GGC TGT GGA CTC AAC GAT 768 He Tyr Pro Asn Gly Gly Asp Phe Gin Pro Gly Cys Gly Leu Asn Asp 595 600 605

GTC TTG GGA TCA ATT GCA TAT GGA ACA ATC ACA GAG GTG GTA AAA TGT 816 Val Leu Gly Ser He Ala Tyr Gly Thr He Thr Glu Val Val Lys Cys 610 615 620 625 GAG CAT GAG CGA GCC GTC CAC CTC TTT GTT GAC TCT CTG GTG AAT CAG 864 Glu His Glu Arg Ala Val His Leu Phe Val Asp Ser Leu Val Asn Gin 630 635 640

GAC AAG CCG AGT TTT GCC TTC CAG TGC ACT GAC TCC AAT CGC TTC AAA 912 Asp Lys Pro Ser Phe Ala Phe Gin Cys Thr Asp Ser Asn Arg Phe Lys 645 650 655

AAG GGG ATC TGT CTG AGC TGC CGC AAG AAC CGT TGT AAT AGC ATT GGC 960 Lys Gly He Cys Leu Ser Cys Arg Lys Asn Arg Cys Asn Ser He Gly 660 665 670

TAC AAT GCC AAG AAA ATG AGG AAC AAG AGG AAC AGC AAA ATG TAC CTA 1008 Tyr Asn Ala Lys Lys Met Arg Asn Lys Arg Asn Ser Lys Met Tyr Leu 675 680 685

AAA ACC CGG GCA GGC ATG CCT TTC AGA GGT AAC CTT CAG TCC CTG GAG 1056 Lys Thr Arg Ala Gly Met Pro Phe Arg Gly Asn Leu Gin Ser Leu Glu 690 695 700 705

TGT CCC TGA 1065

Cys Pro *

(2) INFORMATION FOR SEQ ID NO: 6: (i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 355 amino acids

(B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 6:

Met Ser Asn Ser Val Pro Leu Leu Cys Phe Trp Ser Leu Cys Tyr Cys 1 5 10 15

Phe Ala Ala Gly Ser Pro Val Pro Phe Gly Pro Glu Gly Arg Leu Glu 20 25 30 Asp Lys Leu His Lys Pro Lys Ala Thr Gin Thr Glu Val Lys Pro Ser 35 40 45

Val Arg Phe Asn Leu Arg Thr Ser Lys Asp Pro Glu His Glu Gly Cys 50 55 60

Tyr Leu Ser Val Gly His Ser Gin Pro Leu Glu Asp Cys Ser Phe Asn 65 70 75 80

Met Thr Ala Lys Thr Phe Phe He He His Gly Trp Thr Met Ser Gly 85 90 95

He Phe Glu Asn Trp Leu His Lys Leu Val Ser Ala Leu His Thr Arg 100 105 110 Glu Lys Asp Ala Asn Val Val Val Val Asp Trp Leu Pro Leu Ala His 115 120 125

Gin Leu Tyr Thr Asp Ala Val Asn Asn Thr Arg Val Val Gly His Ser 130 135 140

He Ala Arg Met Leu Asp Trp Leu Gin Glu Lys Asp Asp Phe Ser Leu 145 150 155 160

Gly Asn Val His Leu He Gly Tyr Ser Leu Gly Ala His Val Ala Gly 165 170 175

Tyr Ala Gly Asn Phe Val Lys Gly Thr Val Gly Arg He Thr Gly Leu 180 185 190

Asp Pro Ala Gly Pro Met Phe Glu Gly Ala Asp He His Lys Arg Leu 195 200 205

Ser Pro Asp Asp Ala Asp Phe Val Asp Val Leu His Thr Tyr Thr Arg 210 215 220

Ser Phe Gly Leu Ser He Gly He Gin Met Pro Val Gly His He Asp 225 230 235 240

He Tyr Pro Asn Gly Gly Asp Phe Gin Pro Gly Cys Gly Leu Asn Asp 245 250 255 Val Leu Gly Ser He Ala Tyr Gly Thr He Thr Glu Val Val Lys Cys 260 265 270

Glu His Glu Arg Ala Val His Leu Phe Val Asp Ser Leu Val Asn Gin 275 280 285

Asp Lys Pro Ser Phe Ala Phe Gin Cys Thr Asp Ser Asn Arg Phe Lys 290 295 300

Lys Gly He Cys Leu Ser Cys Arg Lys Asn Arg Cys Asn Ser He Gly 305 310 315 320

Tyr Asn Ala Lys Lys Met Arg Asn Lys Arg Asn Ser Lys Met Tyr Leu

325 330 335 Lys Thr Arg Ala Gly Met Pro Phe Arg Gly Asn Leu Gin Ser Leu Glu

340 345 350

Cys Pro * 355

(2) INFORMATION FOR SEQ ID NO: 7:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 2565 base pairs (B) TYPE: nucleic acid

(C) STRANDEDNESS : double

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: cDNA

(ix) FEATURE:

(A) NAME/KEY: CDS

(B) LOCATION: 252..1754

(xi) SEQUENCE DESCRIPTION: SEQ ID NO : 7 : GAATTCGCGG CCGCGTCGAC GGCGGCTCAG GACGAGGGCA GATCTCGTTC TGGGGCAAGC 60 CGTTGACACT CGCTCCCTGC CACCGCCCGG GCTCCGTGCC GCCAAGTTTT CATTTTCCAC 120

CTTCTCTGCC TCCAGTCCCC CAGCCCCTGG CCGAGAGAAG GGTCTTACCG GCCGGGATTG 180

CTGGAAACAC CAAGAGGTGG TTTTTGTTTT TTAAAACTTC TGTTTCTTGG GAGGGGGTGT 240 GGCGGGGCAG G ATG AGC AAC TCC GTT CCT CTG CTC TGT TTC TGG AGC CTC 290 Met Ser Asn Ser Val Pro Leu Leu Cys Phe Trp Ser Leu 360 365

TGC TAT TGC TTT GCT GCG GGG AGC CCC GTA CCT TTT GGT CCA GAG GGA 338 Cys Tyr Cys Phe Ala Ala Gly Ser Pro Val Pro Phe Gly Pro Glu Gly 370 375 380

CGG CTG GAA GAT AAG CTC CAC AAA CCC AAA GCT ACA CAG ACT GAG GTC 386 Arg Leu Glu Asp Lys Leu His Lys Pro Lys Ala Thr Gin Thr Glu Val 385 390 395 400

AAA CCA TCT GTG AGG TTT AAC CTC CGC ACC TCC AAG GAC CCA GAG CAT 434 Lys Pro Ser Val Arg Phe Asn Leu Arg Thr Ser Lys Asp Pro Glu His 405 410 415

GAA GGA TGC TAC CTC TCC GTC GGC CAC AGC CAG CCC TTA GAA GAC TGC 482 Glu Gly Cys Tyr Leu Ser Val Gly His Ser Gin Pro Leu Glu Asp Cys 420 425 430 AGT TTC AAC ATG ACA GCT AAA ACC TTT TTC ATC ATT CAC GGA TGG ACG 530 Ser Phe Asn Met Thr Ala Lys Thr Phe Phe He He His Gly Trp Thr 435 440 445

ATG AGC GGT ATC TTT GAA AAC TGG CTG CAC AAA CTC GTG TCA GCC CTG 578 Met Ser Gly He Phe Glu Asn Trp Leu His Lys Leu Val Ser Ala Leu 450 455 460

CAC ACA AGA GAG AAA GAC GCC AAT GTA GTT GTG GTT GAC TGG CTC CCC 626 His Thr Arg Glu Lys Asp Ala Asn Val Val Val Val Asp Trp Leu Pro 465 470 475 480

CTG GCC CAC CAG CTT TAC ACG GAT GCG GTC AAT AAT ACC AGG GTG GTG 674 Leu Ala His Gin Leu Tyr Thr Asp Ala Val Asn Asn Thr Arg Val Val 485 490 495

GGA CAC AGC ATT GCC AGG ATG CTC GAC TGG CTG CAG GAG AAG GAC GAT 722 Gly His Ser He Ala Arg Met Leu Asp Trp Leu Gin Glu Lys Asp Asp 500 505 510 TTT TCT CTC GGG AAT GTC CAC TTG ATC GGC TAC AGC CTC GGA GCG CAC 770 Phe Ser Leu Gly Asn Val His Leu He Gly Tyr Ser Leu Gly Ala His 515 520 525 GTG GCC GGG TAT GCA GGC AAC TTC GTG AAA GGA ACG GTG GGC CGA ATC 818

Val Ala Gly Tyr Ala Gly Asn Phe Val Lys Gly Thr Val Gly Arg He 530 535 540

ACA GGT TTG GAT CCT GCC GGG CCC ATG TTT GAA GGG GCC GAC ATC CAC 866

Thr Gly Leu Asp Pro Ala Gly Pro Met Phe Glu Gly Ala Asp He His

545 550 555 560 AAG AGG CTC TCT CCG GAC GAT GCA GAT TTT GTG GAT GTC CTC CAC ACC 914

Lys Arg Leu Ser Pro Asp Asp Ala Asp Phe Val Asp Val Leu His Thr

565 570 575

TAC ACG CGT TCC TTC GGC TTG AGC ATT GGT ATT CAG ATG CCT GTG GGC 962 Tyr Thr Arg Ser Phe Gly Leu Ser He Gly He Gin Met Pro Val Gly 580 585 590

CAC ATT GAC ATC TAC CCC AAT GGG GGT GAC TTC CAG CCA GGC TGT GGA 1010

His He Asp He Tyr Pro Asn Gly Gly Asp Phe Gin Pro Gly Cys Gly 595 600 605

CTC AAC GAT GTC TTG GGA TCA ATT GCA TAT GGA ACA ATC ACA GAG GTG 1058

Leu Asn Asp Val Leu Gly Ser He Ala Tyr Gly Thr He Thr Glu Val 610 615 620

GTA AAA TGT GAG CAT GAG CGA GCC GTC CAC CTC TTT GTT GAC TCT CTG 1106

Val Lys Cys Glu His Glu Arg Ala Val His Leu Phe Val Asp Ser Leu

625 630 635 640 GTG AAT CAG GAC AAG CCG AGT TTT GCC TTC CAG TGC ACT GAC TCC AAT 1154

Val Asn Gin Asp Lys Pro Ser Phe Ala Phe Gin Cys Thr Asp Ser Asn

645 650 655

CGC TTC AAA AAG GGG ATC TGT CTG AGC TGC CGC AAG AAC CGT TGT AAT 1202 Arg Phe Lys Lys Gly He Cys Leu Ser Cys Arg Lys Asn Arg Cys Asn 660 665 670

AGC ATT GGC TAC AAT GCC AAG AAA ATG AGG AAC AAG AGG AAC AGC AAA 1250

Ser He Gly Tyr Asn Ala Lys Lys Met Arg Asn Lys Arg Asn Ser Lys 675 680 685

ATG TAC CTA AAA ACC CGG GCA GGC ATG CCT TTC AGA GTT TAC CAT TAT 1298

Met Tyr Leu Lys Thr Arg Ala Gly Met Pro Phe Arg Val Tyr His Tyr 690 695 700

CAG ATG AAA ATC CAT GTC TTC AGT TAC AAG AAC ATG GGA GAA ATT GAG 1346

Gin Met Lys He His Val Phe Ser Tyr Lys Asn Met Gly Glu He Glu

705 710 715 720 CCC ACC TTT TAC GTC ACC CTT TAT GGC ACT AAT GCA GAT TCC CAG ACT 1394

Pro Thr Phe Tyr Val Thr Leu Tyr Gly Thr Asn Ala Asp Ser Gin Thr

725 730 735 CTG CCA CTG GAA ATA GTG GAG CGG ATC GAG CAG AAT GCC ACC AAC ACC 1442

Leu Pro Leu Glu He Val Glu Arg He Glu Gin Asn Ala Thr Asn Thr 740 745 750

TTC CTG GTC TAC ACC GAG GAG GAC TTG GGA GAC CTC TTG AAG ATC CAG 1490

Phe Leu Val Tyr Thr Glu Glu Asp Leu Gly Asp Leu Leu Lys He Gin 755 760 765 CTC ACC TGG GAG GGG GCC TCT CAG TCT TGG TAC AAC CTG TGG AAG GAG 1538 Leu Thr Trp Glu Gly Ala Ser Gin Ser Trp Tyr Asn Leu Trp Lys Glu 770 775 780

TTT CGC AGC TAC CTG TCT CAA CCC CGC AAC CCC GGA CGG GAG CTG AAT 1586 Phe Arg Ser Tyr Leu Ser Gin Pro Arg Asn Pro Gly Arg Glu Leu Asn

785 790 795 800

ATC AGG CGC ATC CGG GTG AAG TCT GGG GAA ACC CAG CGG AAA CTG ACA 1634

He Arg Arg He Arg Val Lys Ser Gly Glu Thr Gin Arg Lys Leu Thr 805 810 815

TTT TGT ACA GAA GAC CCT GAG AAC ACC AGC ATA TCC CCA GGC CGG GAG 1682

Phe Cys Thr Glu Asp Pro Glu Asn Thr Ser He Ser Pro Gly Arg Glu 820 825 830

CTC TGG TTT CGC AAG TGT CGG GAT GGC TGG AGG ATG AAA AAC GAA ACC 1730

Leu Trp Phe Arg Lys Cys Arg Asp Gly Trp Arg Met Lys Asn Glu Thr 835 840 845 AGT CCC ACT GTG GAG CTT CCC TGA GGGTGCCCGG GCAAGTCTTG CCAGCAAGGC 1784

Ser Pro Thr Val Glu Leu Pro * 850 855

AGCAAGACTT CCTGCTATCC AAGCCCATGG AGGAAAGTTA CTGCTGAGGA CCCACCCAAT 1844

GGAAGGATTC TTCTCAGCCT TGACCCTGGA GCACTGGGAA CAACTGGTCT CCTGTGATGG 1904

CTGGGACTCC TCGCGGGAGG GGACTGCGCT GCTATAGCTC TTGCTGCCTC TCTTGAATAG 1964 CTCTAACTCC AAACCTCTGT CCACACCTCC AGAGCACCAA GTCCAGATTT GTGTGTAAGC 2024

AGCTGGGTGC CTGGGGCCTC TCGTGCACAC TGGATTGGTT TCTCAGTTGC TGGGCGAGCC 2084

TGTACTCTGC CTGACGAGGA ACGCTGGCTC CGAAGAGGCC CTGTGTAGAA GGCTGTCAGC 2144

TGCTCAGCCT GCTTTGAGCC TCAGTGAGAA GTCCTTCCGA CAGGAGCTGA CTCATGTCAG 2204

GATGGCAGGC CTGGTATCTT GCTCGGGCCC TGGCTGTTGG GGTTCTCATG GGTTGCACTG 2264 ACCATACTGC TTACGTCTTA GCCATTCCGT CCTGCTCCCC AGCTCACTCT CTGAAGCACA 2324

CATCATTGGC TTTCCTATTT TTCTGTTCAT TTTTTAATTG AGCAAATGTC TATTGAACAC 2384 TTAAAATTAA TTAGAATGTG GTAATGGACA TATTACTGAG CCTCTCCATT TGGAACCCAG 2444 TGGAGTTGGG ATTTCTAGAC CCTCTTTCTG TTTGGATGGT GTATGTGTAT ATGCATGGGG 2504

AAAGGCACCT GGGGCCTGGG GGAGGCTATA GGATATAAGC AGTCGACGCG GCCGCGAATT 2564 C 2565

(2) INFORMATION FOR SEQ ID NO: 8:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 501 amino acids (B) TYPE: amino acid

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 8:

Met Ser Asn Ser Val Pro Leu Leu Cys Phe Trp Ser Leu Cys Tyr Cys 1 5 10 15 Phe Ala Ala Gly Ser Pro Val Pro Phe Gly Pro Glu Gly Arg Leu Glu

20 25 30

Asp Lys Leu His Lys Pro Lys Ala Thr Gin Thr Glu Val Lys Pro Ser 35 40 45

Val Arg Phe Asn Leu Arg Thr Ser Lys Asp Pro Glu His Glu Gly Cys 50 55 60

Tyr Leu Ser Val Gly His Ser Gin Pro Leu Glu Asp Cys Ser Phe Asn 65 70 75 80

Met Thr Ala Lys Thr Phe Phe He He His Gly Trp Thr Met Ser Gly 85 90 95 He Phe Glu Asn Trp Leu His Lys Leu Val Ser Ala Leu His Thr Arg 100 105 110

Glu Lys Asp Ala Asn Val Val Val Val Asp Trp Leu Pro Leu Ala His 115 120 125

Gin Leu Tyr Thr Asp Ala Val Asn Asn Thr Arg Val Val Gly His Ser 130 135 140

He Ala Arg Met Leu Asp Trp Leu Gin Glu Lys Asp Asp Phe Ser Leu 145 150 155 160

Gly Asn Val His Leu He Gly Tyr Ser Leu Gly Ala His Val Ala Gly 165 170 175

Tyr Ala Gly Asn Phe Val Lys Gly Thr Val Gly Arg He Thr Gly Leu 180 185 190

Asp Pro Ala Gly Pro Met Phe Glu Gly Ala Asp He His Lys Arg Leu 195 200 205

Ser Pro Asp Asp Ala Asp Phe Val Asp Val Leu His Thr Tyr Thr Arg 210 215 220

Ser Phe Gly Leu Ser He Gly He Gin Met Pro Val Gly His He Asp 225 230 235 240 He Tyr Pro Asn Gly Gly Asp Phe Gin Pro Gly Cys Gly Leu Asn Asp

245 250 255

Val Leu Gly Ser He Ala Tyr Gly Thr He Thr Glu Val Val Lys Cys 260 265 270

Glu His Glu Arg Ala Val His Leu Phe Val Asp Ser Leu Val Asn Gin 275 280 285

Asp Lys Pro Ser Phe Ala Phe Gin Cys Thr Asp Ser Asn Arg Phe Lys 290 295 300

Lys Gly He Cys Leu Ser Cys Arg Lys Asn Arg Cys Asn Ser He Gly 305 310 315 320 Tyr Asn Ala Lys Lys Met Arg Asn Lys Arg Asn Ser Lys Met Tyr Leu

325 330 335

Lys Thr Arg Ala Gly Met Pro Phe Arg Val Tyr His Tyr Gin Met Lys

340 345 350

He His Val Phe Ser Tyr Lys Asn Met Gly Glu He Glu Pro Thr Phe

355 360 365

Tyr Val Thr Leu Tyr Gly Thr Asn Ala Asp Ser Gin Thr Leu Pro Leu 370 375 380

Glu He Val Glu Arg He Glu Gin Asn Ala Thr Asn Thr Phe Leu Val 385 390 395 400 Tyr Thr Glu Glu Asp Leu Gly Asp Leu Leu Lys He Gin Leu Thr Trp

405 410 415

Glu Gly Ala Ser Gin Ser Trp Tyr Asn Leu Trp Lys Glu Phe Arg Ser 420 425 430

Tyr Leu Ser Gin Pro Arg Asn Pro Gly Arg Glu Leu Asn He Arg Arg 435 440 445 He Arg Val Lys Ser Gly Glu Thr Gin Arg Lys Leu Thr Phe Cys Thr 450 455 460

Glu Asp Pro Glu Asn Thr Ser He Ser Pro Gly Arg Glu Leu Trp Phe 465 470 475 480

Arg Lys Cys Arg Asp Gly Trp Arg Met Lys Asn Glu Thr Ser Pro Thr 485 490 495

Val Glu Leu Pro * 500

(2) INFORMATION FOR SEQ ID NO: 9:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1035 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS : double (D) TOPOLOGY: linear

(ii) MOLECULE TYPE: cDNA

(ix) FEATURE:

(A) NAME/KEY: CDS

(B) LOCATION: 1..1035

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 9:

ATG AGC AAC TCC GTT CCT CTG CTC TGT TTC TGG AGC CTC TGC TAT TGC 48 Met Ser Asn Ser Val Pro Leu Leu Cys Phe Trp Ser Leu Cys Tyr Cys 505 510 515

TTT GCT GCG GGG AGC CCC GTA CCT TTT GGT CCA GAG GGA CGG CTG GAA 96 Phe Ala Ala Gly Ser Pro Val Pro Phe Gly Pro Glu Gly Arg Leu Glu 520 525 530 GAT AAG CTC CAC AAA CCC AAA GCT ACA CAG ACT GAG GTC AAA CCA TCT 144 Asp Lys Leu His Lys Pro Lys Ala Thr Gin Thr Glu Val Lys Pro Ser 535 540 545

GTG AGG TTT AAC CTC CGC ACC TCC AAG GAC CCA GAG CAT GAA GGA TGC 192 Val Arg Phe Asn Leu Arg Thr Ser Lys Asp Pro Glu His Glu Gly Cys 550 555 560 565

TAC CTC TCC GTC GGC CAC AGC CAG CCC TTA GAA GAC TGC AGT TTC AAC 240 Tyr Leu Ser Val Gly His Ser Gin Pro Leu Glu Asp Cys Ser Phe Asn 570 575 580

ATG ACA GCT AAA ACC TTT TTC ATC ATT CAC GGA TGG ACG ATG AGC GGT 288 Met Thr Ala Lys Thr Phe Phe He He His Gly Trp Thr Met Ser Gly

585 590 595

ATC TTT GAA AAC TGG CTG CAC AAA CTC GTG TCA GCC CTG CAC ACA AGA 336 He Phe Glu Asn Trp Leu His Lys Leu Val Ser Ala Leu His Thr Arg 600 605 610

GAG AAA GAC GCC AAT GTA GTT GTG GTT GAC TGG CTC CCC CTG GCC CAC 384

Glu Lys Asp Ala Asn Val Val Val Val Asp Trp Leu Pro Leu Ala His 615 620 625

CAG CTT TAC ACG GAT GCG GTC AAT AAT ACC AGG GTG GTG GGA CAC AGC 432

Gin Leu Tyr Thr Asp Ala Val Asn Asn Thr Arg Val Val Gly His Ser

630 635 640 645

ATT GCC AGG ATG CTC GAC TGG CTG CAG GAG AAG GAC GAT TTT TCT CTC 480

He Ala Arg Met Leu Asp Trp Leu Gin Glu Lys Asp Asp Phe Ser Leu

650 655 660 GGG AAT GTC CAC TTG ATC GGC TAC AGC CTC GGA GCG CAC GTG GCC GGG 528

Gly Asn Val His Leu He Gly Tyr Ser Leu Gly Ala His Val Ala Gly

665 670 675

TAT GCA GGC AAC TTC GTG AAA GGA ACG GTG GGC CGA ATC ACA GGT TTG 576 Tyr Ala Gly Asn Phe Val Lys Gly Thr Val Gly Arg He Thr Gly Leu 680 685 690

GAT CCT GCC GGG CCC ATG TTT GAA GGG GCC GAC ATC CAC AAG AGG CTC 624

Asp Pro Ala Gly Pro Met Phe Glu Gly Ala Asp He His Lys Arg Leu 695 700 705

TCT CCG GAC GAT GCA GAT TTT GTG GAT GTC CTC CAC ACC TAC ACG CGT 672

Ser Pro Asp Asp Ala Asp Phe Val Asp Val Leu His Thr Tyr Thr Arg

710 715 720 725

TCC TTC GGC TTG AGC ATT GGT ATT CAG ATG CCT GTG GGC CAC ATT GAC 720

Ser Phe Gly Leu Ser He Gly He Gin Met Pro Val Gly His He Asp

730 735 740 ATC TAC CCC AAT GGG GGT GAC TTC CAG CCA GGC TGT GGA CTC AAC GAT 768

He Tyr Pro Asn Gly Gly Asp Phe Gin Pro Gly Cys Gly Leu Asn Asp

745 750 755

GTC TTG GGA TCA ATT GCA TAT GGA ACA ATC ACA GAG GTG GTA AAA TGT 816 Val Leu Gly Ser He Ala Tyr Gly Thr He Thr Glu Val Val Lys Cys 760 765 770

GAG CAT GAG CGA GCC GTC CAC CTC TTT GTT GAC TCT CTG GTG AAT CAG 864

Glu His Glu Arg Ala Val His Leu Phe Val Asp Ser Leu Val Asn Gin 775 780 785

GAC AAG CCG AGT TTT GCC TTC CAG TGC ACT GAC TCC AAT CGC TTC AAA 912 Asp Lys Pro Ser Phe Ala Phe Gin Cys Thr Asp Ser Asn Arg Phe Lys 790 795 800 805

AAG GGG ATC TGT CTG AGC TGC CGC AAG AAC CGT TGT AAT AGC ATT GGC 960 Lys Gly He Cys Leu Ser Cys Arg Lys Asn Arg Cys Asn Ser He Gly

810 815 820

TAC AAT GCC AAG AAA ATG AGG AAC AAG AGG AAC AGC AAA ATG TAC CTA 1008 Tyr Asn Ala Lys Lys Met Arg Asn Lys Arg Asn Ser Lys Met Tyr Leu 825 830 835

AAA ACC CGG GCA GGC ATG CCT TTC AGA 1035

Lys Thr Arg Ala Gly Met Pro Phe Arg 840 845

(2) INFORMATION FOR SEQ ID NO: 10:

(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 345 amino acids

(B) TYPE: amino acid (D) TOPOLOGY: linear

(ii) MOLECULE TYPE: protein

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 10:

Met Ser Asn Ser Val Pro Leu Leu Cys Phe Trp Ser Leu Cys Tyr Cys 1 5 10 15

Phe Ala Ala Gly Ser Pro Val Pro Phe Gly Pro Glu Gly Arg Leu Glu 20 25 30

Asp Lys Leu His Lys Pro Lys Ala Thr Gin Thr Glu Val Lys Pro Ser 35 40 45

Val Arg Phe Asn Leu Arg Thr Ser Lys Asp Pro Glu His Glu Gly Cys 50 55 60 Tyr Leu Ser Val Gly His Ser Gin Pro Leu Glu Asp Cys Ser Phe Asn 65 70 75 80

Met Thr Ala Lys Thr Phe Phe He He His Gly Trp Thr Met Ser Gly 85 90 95

He Phe Glu Asn Trp Leu His Lys Leu Val Ser Ala Leu His Thr Arg 100 105 110

Glu Lys Asp Ala Asn Val Val Val Val Asp Trp Leu Pro Leu Ala His 115 120 125

Gin Leu Tyr Thr Asp Ala Val Asn Asn Thr Arg Val Val Gly His Ser ά> /

130 135 140

He Ala Arg Met Leu Asp Trp Leu Gin Glu Lys Asp Asp Phe Ser Leu 145 150 155 160

Gly Asn Val His Leu He Gly Tyr Ser Leu Gly Ala His Val Ala Gly 165 170 175

Tyr Ala Gly Asn Phe Val Lys Gly Thr Val Gly Arg He Thr Gly Leu 180 185 190

Asp Pro Ala Gly Pro Met Phe Glu Gly Ala Asp He His Lys Arg Leu 195 200 205 Ser Pro Asp Asp Ala Asp Phe Val Asp Val Leu His Thr Tyr Thr Arg 210 215 220

Ser Phe Gly Leu Ser He Gly He Gin Met Pro Val Gly His He Asp

225 230 235 240

He Tyr Pro Asn Gly Gly Asp Phe Gin Pro Gly Cys Gly Leu Asn Asp

245 250 255

Val Leu Gly Ser He Ala Tyr Gly Thr He Thr Glu Val Val Lys Cys 260 265 270

Glu His Glu Arg Ala Val His Leu Phe Val Asp Ser Leu Val Asn Gin 275 280 285 Asp Lys Pro Ser Phe Ala Phe Gin Cys Thr Asp Ser Asn Arg Phe Lys 290 295 300

Lys Gly He Cys Leu Ser Cys Arg Lys Asn Arg Cys Asn Ser He Gly

305 310 315 320

Tyr Asn Ala Lys Lys Met Arg Asn Lys Arg Asn Ser Lys Met Tyr Leu 325 330 335

Lys Thr Arg Ala Gly Met Pro Phe Arg 340 345

(2) INFORMATION FOR SEQ ID NO: 11:

(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 225 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS : double

(D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA <£«£<

( ix ) FEATURE :

(A) NAME/KEY: CDS

(B) LOCATION: 1..225

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 11:

CTG GGA TCC ATC GCC TAT GGC ACG ATC GCG GAG GTG GTG AAG TGC GAG 48 Leu Gly Ser He Ala Tyr Gly Thr He Ala Glu Val Val Lys Cys Glu 350 355 360

CAT GAG CGG GCC GTG CAT CTC TTT GTG GAC TCC CTG GTG AAC CAG GAC 96 His Glu Arg Ala Val His Leu Phe Val Asp Ser Leu Val Asn Gin Asp 365 370 375

AAG CCG AGC TTT GCC TTC CAG TGC ACA GAC TCC AAC CGC TTC AAA AAA 144 Lys Pro Ser Phe Ala Phe Gin Cys Thr Asp Ser Asn Arg Phe Lys Lys 380 385 390 GGG ATC TGT CTC AGC TGC CGG AAG AAC CGC TGT AAC GGC ATC GGC TAC 192 Gly He Cys Leu Ser Cys Arg Lys Asn Arg Cys Asn Gly He Gly Tyr 395 400 405

AAT GCT AAG AAG ACG AGG AAT AAG AGG AAC ACC 225 Asn Ala Lys Lys Thr Arg Asn Lys Arg Asn Thr 410 415 420

(2) INFORMATION FOR SEQ ID NO: 12:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 75 amino acids

(B) TYPE: amino acid (D) TOPOLOGY: linear

(ii) MOLECULE TYPE: protein

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 12: Leu Gly Ser He Ala Tyr Gly Thr He Ala Glu Val Val Lys Cys Glu 1 5 10 15

His Glu Arg Ala Val His Leu Phe Val Asp Ser Leu Val Asn Gin Asp 20 25 30

Lys Pro Ser Phe Ala Phe Gin Cys Thr Asp Ser Asn Arg Phe Lys Lys 35 40 45

Gly He Cys Leu Ser Cys Arg Lys Asn Arg Cys Asn Gly He Gly Tyr 50 55 60

Asn Ala Lys Lys Thr Arg Asn Lys Arg Asn Thr 65 70 75

(2) INFORMATION FOR SEQ ID NO: 13:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 472 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS :

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: protein

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 13:

Met Glu Ser Lys Ala Leu Leu Val Leu Thr Leu Ala Val Trp Leu Gin 1 5 10 15

Ser Leu Thr Ala Ser Arg Gly Gly Val Ala Ala Ala Asp Gin Arg Arg 20 25 30

Asp Phe He Asp He Glu Ser Lys Phe Ala Leu Arg Thr Pro Glu Asp 35 40 45

Thr Ala Glu Asp Thr Cys His Leu He Pro Gly Val Ala Glu Ser Val 50 55 60

Ala Thr Cys His Phe Asn His Ser Ser Lys Thr Phe Met Val He His 65 70 75 80

Gly Trp Thr Val Thr Gly Met Tyr Glu Ser Trp Val Pro Lys Leu Val 85 90 95

Ala Ala Leu Tyr Lys Arg Glu Pro Asp Ser Asn Val He Val Val Asp 100 105 110

Trp Leu Ser Arg Ala Gin Glu His Tyr Pro Val Ser Ala Gly Tyr Thr 115 120 125

Lys Val Gly Gin Asp Val Ala Arg Phe He Asn Trp Met Glu Glu Glu 130 135 140 Phe Asn Tyr Pro Leu Asp Asn Val His Leu Leu Gly Tyr Ser Leu Gly 145 150 155 160

Ala His Ala Ala Gly He Ala Gly Ser Leu Thr Asn Lys Lys Val Asn 165 170 175

Arg He Thr Gly Leu Asp Pro Ala Gly Pro Asn Phe Glu Tyr Ala Glu 180 185 190 •

Ala Pro Arg Leu Ser Pro Asp Asp Ala Asp Phe Val Asp Val Leu His 195 200 205

Thr Phe Thr Arg Gly Ser Pro Gly Arg Ser He Gly He Gin Lys Pro 210 215 220

Val Gly His Val Asp He Tyr Pro Asn Gly Gly Thr Phe Gin Pro Gly 225 230 235 240

Cys Asn He Gly Glu Ala He Arg Val He Ala Glu Arg Gly Leu Gly 245 250 255

Asp Val Asp Gin Leu Lys Cys Ser His Glu Arg Ser He His Leu Phe 260 265 270

He Asp Ser Leu Leu Asn Glu Glu Asn Pro Ser Lys Ala Tyr Arg Cys 275 280 285 Ser Ser Lys Glu Ala Phe Glu Lys Gly Leu Cys Leu Ser Cys Arg Lys 290 295 300

Asn Arg Cys Asn Asn Leu Gly Tyr Glu He Asn Lys Val Arg Ala Lys

305 310 315 320

Arg Ser Ser Lys Met Tyr Leu Lys Thr Arg Ser Gin Met Pro Tyr Lys

325 330 335

Val Phe His Tyr Gin Val Lys He His Phe Ser Gly Thr Glu Ser Glu 340 345 350

Thr His Thr Asn Gin Ala Phe Glu He Ser Leu Tyr Gly Thr Val Ala 355 360 365 Glu Ser Glu Asn He Pro Phe Thr Leu Pro Glu Val Ser Thr Asn Lys 370 375 380

Thr Tyr Ser Phe Leu He Tyr Thr Glu Val Asp He Gly Glu Leu Leu 385 390 395 400

Met Leu Lys Leu Lys Trp Lys Ser Asp Ser Tyr Phe Ser Trp Ser Asp 405 410 415

Trp Trp Ser Ser Pro Gly Phe Ala He Gin Lys He Arg Val Lys Ala 420 425 430

Gly Glu Thr Gin Lys Lys Val He Phe Cys Ser Arg Glu Lys Val Ser 435 440 445

His Leu Gin Lys Gly Lys Ala Pro Ala Val Phe Val Lys Cys His Asp 450 455 460 ^-r

Lys Ser Leu Asn Lys Lys Ser Gly 465 470

(2) INFORMATION FOR SEQ ID NO: 14:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 499 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS : (D) TOPOLOGY: linear

(ii) MOLECULE TYPE: protein

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 14:

Met Asp Thr Ser Pro Leu Cys Phe Ser He Leu Leu Val Leu Cys He 1 5 10 15

Phe He Gin Ser Ser Ala Leu Gly Gin Ser Leu Lys Pro Glu Pro Phe 20 25 30 Gly Arg Arg Ala Gin Ala Val Glu Thr Asn Lys Thr Leu His Glu Met

35 40 45

Lys Thr Arg Phe Leu Leu Phe Gly Glu Thr Asn Gin Gly Cys Gin He 50 55 60

Arg He Asn His Pro Asp Thr Leu Gin Glu Cys Gly Phe Asn Ser Ser 65 70 75 80

Leu Pro Leu Val Met He He His Gly Trp Ser Val Asp Gly Val Leu 85 90 95

Glu Asn Trp He Trp Gin Met Val Ala Ala Leu Lys Ser Gin Pro Ala 100 105 110 Gin Pro Val Asn Val Gly Leu Val Asp Trp He Thr Leu Ala His Asp

115 120 125

His Tyr Thr He Ala Val Arg Asn Thr Arg Leu Val Gly Lys Glu Val 130 135 140

Ala Ala Leu Leu Arg Trp Leu Glu Glu Ser Val Gin Leu Ser Arg Ser 145 150 155 160

His Val His Leu He Gly Tyr Ser Leu Gly Ala His Val Ser Gly Phe 165 170 175

Ala Gly Ser Ser He Gly Gly Thr His Lys He Gly Arg He Thr Gly 180 185 190

Leu Asp Ala Ala Gly Pro Leu Phe Glu Gly Ser Ala Pro Ser Asn Arg 195 200 205

Leu Ser Pro Asp Asp Ala Asn Phe Val Asp Ala He His Thr Phe Thr 210 215 220

Arg Glu His Met Gly Leu Ser Val Gly He Lys Gin Pro He Gly His 225 230 235 240

Tyr Asp Phe Tyr Pro Asn Gly Gly Ser Phe Gin Pro Gly Cys His Phe 245 250 255 Leu Glu Leu Tyr Arg His He Ala Gin His Gly Phe Asn Ala He Thr

260 265 270

Gin Thr He Lys Cys Ser His Glu Arg Ser Val His Leu Phe He Asp 275 280 285

Ser Leu Leu His Ala Gly Thr Gin Ser Met Ala Tyr Pro Cys Gly Asp 290 295 300

Met Asn Ser Phe Ser Gin Gly Leu Cys Leu Ser Cys Lys Lys Gly Arg 305 310 315 320

Cys Asn Thr Leu Gly Tyr His Val Arg Gin Glu Pro Arg Ser Lys Ser

325 330 335 Lys Arg Leu Phe Leu Val Thr Arg Ala Gin Ser Pro Phe Lys Val Tyr

340 345 350

His Tyr Gin Leu Lys He Gin Phe He Asn Gin Thr Glu Thr Pro He 355 360 365

Gin Thr Thr Phe Thr Met Ser Leu Leu Gly Thr Lys Glu Lys Met Gin 370 375 380

Lys He Pro He Thr Leu Gly Lys Gly He Ala Ser Asn Lys Thr Tyr 385 390 395 400

Ser Phe Leu He Thr Leu Asp Val Asp He Gly Glu Leu He Met He 405 410 415 Lys Phe Lys Trp Glu Asn Ser Ala Val Trp Ala Asn Val Trp Asp Thr

420 425 430

Val Gin Thr He He Pro Trp Ser Thr Gly Pro Arg His Ser Gly Leu 435 440 445

Val Leu Lys Thr He Arg Val Lys Ala Gly Glu Thr Gin Gin Arg Met 450 455 460 ά 7

Thr Phe Cys Ser Glu Asn Thr Asp Asp Leu Leu Leu Arg Pro Thr Gin 465 470 475 480

Glu Lys He Phe Val Lys Cys Glu He Lys Ser Lys Thr Ser Lys Arg 485 490 495

Lys He Arg

(2) INFORMATION FOR SEQ ID NO: 15:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 465 amino acids (B) TYPE: amino acid

(C) STRANDEDNESS :

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: protein

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 15:

Met Leu Pro Leu Trp Thr Leu Ser Leu Leu Leu Gly Ala Val Ala Gly 1 5 10 15

Lys Glu Val Cys Tyr Glu Arg Leu Gly Cys Phe Ser Asp Asp Ser Pro 20 25 30

Trp Ser Gly He Thr Glu Arg Pro Leu His He Leu Pro Trp Ser Pro 35 40 45 Lys Asp Val Asn Thr Arg Phe Leu Leu Tyr Thr Asn Glu Asn Pro Asn 50 55 60

Asn Phe Gin Glu Val Ala Ala Asp Ser Ser Ser He Ser Gly Ser Asn 65 70 75 80

Phe Lys Thr Asn Arg Lys Thr Arg Phe He He His Gly Phe He Asp 85 90 95

Lys Gly Glu Glu Asn Trp Leu Ala Asn Val Cys Lys Asn Leu Phe Lys 100 105 110

Val Glu Ser Val Asn Cys He Cys Val Asp Trp Lys Gly Gly Ser Arg 115 120 125 Thr Gly Tyr Thr Gin Ala Ser Gin Asn He Arg He Val Gly Ala Glu 130 135 140 Val Ala Tyr Phe Val Glu Phe Leu Gin Ser Ala Phe Gly Tyr Ser Pro 145 150 155 160

Ser Asn Val His Val He Gly His Ser Leu Gly Ala His Ala Ala Gly 165 170 175

Glu Ala Gly Arg Arg Thr Asn Gly Thr He Gly Arg He Thr Gly Leu 180 185 190

Asp Pro Ala Glu Pro Cys Phe Gin Gly Thr Pro Glu Leu Val Arg Leu 195 200 205

Asp Pro Ser Asp Ala Lys Phe Val Asp Val He His Thr Asp Gly Ala 210 215 220

Pro He Val Pro Asn Leu Gly Phe Gly Met Ser Gin Val Val Gly His

225 230 235 240

Leu Asp Phe Phe Pro Asn Gly Gly Val Glu Met Pro Gly Cys Lys Lys 245 250 255

Asn He Leu Ser Gin He Val Asp He Asp Gly He Trp Glu Gly Thr 260 265 270 Arg Asp Phe Ala Ala Cys Asn His Leu Arg Ser Tyr Lys Tyr Tyr Thr

275 280 285

Asp Ser He Val Asn Pro Asp Gly Phe Ala Gly Phe Pro Cys Ala Ser 290 295 300

Tyr Asn Val Phe Thr Ala Asn Lys Cys Phe Pro Cys Pro Ser Gly Gly 305 310 315 320

Cys Pro Gin Met Gly His Tyr Ala Asp Arg Tyr Pro Gly Lys Thr Asn 325 330 335

Asp Val Gly Gin Lys Phe Tyr Leu Asp Thr Gly Asp Ala Ser Asn Phe 340 345 350 Ala Arg Trp Arg Tyr Lys Val Ser Val Thr Leu Ser Gly Lys Lys Val

355 360 365

Thr Gly His He Leu Val Ser Leu Phe Gly Asn Lys Gly Asn Ser Lys 370 375 380

Gin Tyr Glu He Phe Lys Gly Thr Leu Lys Pro Asp Ser Thr His Ser 385 390 395 400

Asn Glu Phe Asp Ser Asp Val Asp Val Gly Asp Leu Gin Met Val Lys 405 410 415

Phe He Trp Tyr Asn Asn Val He Asn Pro Thr Leu Pro Arg Val Gly 420 425 430

Ala Ser Lys He He Val Glu Thr Asn Val Gly Lys Gin Phe Asn Phe 435 440 445

Cys Ser Pro Glu Thr Val Arg Glu Glu Val Leu Leu Thr Leu Thr Pro 450 455 460

Cys 465

(2) INFORMATION FOR SEQ ID NO: 16:

(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 17 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS :

(D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (v) FRAGMENT TYPE: internal

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 16:

Gly Pro Glu Gly Arg Leu Glu Asp Lys Leu His Lys Pro Lys Ala Thr 1 5 10 15

Cys

(2) INFORMATION FOR SEQ ID NO: 17:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 13 base pairs

(B) TYPE: nucleic acid (C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: other nucleic acid

(A) DESCRIPTION: /desc = "Oligonucleotide'

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 17: TTTTTTTTTT TGA 13 o

(2) INFORMATION FOR SEQ ID NO: 18:

(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 10 base pairs (B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc = "Oligonucleotide"

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:18:

GATCAATCGC 10

(2) INFORMATION FOR SEQ ID NO: 19:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 23 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single (D) TOPOLOGY: linear

(ii) MOLECULE TYPE: other nucleic acid

(A) DESCRIPTION: /desc = "Oligonucleotide"

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 19: TAGGACATGC ACAGTGTAAT CTG 23

(2) INFORMATION FOR SEQ ID NO: 20:

(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear (ii) MOLECULE TYPE: other nucleic acid

(A) DESCRIPTION: /desc = "Oligonucleotide"

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:20: 7/

GATTGTGCTG GCCACTTCTC 20

(2) INFORMATION FOR SEQ ID NO: 21:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 19 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: other nucleic acid

(A) DESCRIPTION: /desc = "Oligonucleotide"

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 21: GACACTCCAG GGACTGAAG 19

(2) INFORMATION FOR SEQ ID NO: 22:

(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 48 base pairs (B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc = "Oligonucleotide"

(ix) FEATURE:

(A) NAME/KEY: modified_base (B) LOCATION: 36

(D) OTHER INFORMATION: /mod_base=

(ix) FEATURE:

(A) NAME/KEY: modified_base (B) LOCATION: 37

(D) OTHER INFORMATION: /mod_base=

( ix) FEATURE :

(A) NAME/KEY: modified_base (B) LOCATION: 41

(D) OTHER INFORMATION: /mod_base=

(ix) FEATURE:

(A) NAME/KEY: modified_base (B) LOCATION: 42

(D) OTHER INFORMATION: /mod_base= ( ix) FEATURE : 7

(A) NAME/KEY: modified_base

(B) LOCATION: 46

(D) OTHER INFORMATION: /mod_base= i

(ix) FEATURE:

(A) NAME/KEY: modified_base

(B) LOCATION: 47

(D) OTHER INFORMATION: /mod_base= i

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 22: CUACUACUAC UAGGCCACGC GTCGACTAGT ACGGGNNGGG NNGGGNNG (2) INFORMATION FOR SEQ ID NO: 23:

(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 28 base pairs (B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc = "Oligonucleotide"

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:23:

CACACACAGG CCACGCGTCG ACTAGTAC 28

(2) INFORMATION FOR SEQ ID NO: 24:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 24 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single (D) TOPOLOGY: linear

(ii) MOLECULE TYPE: other nucleic acid

(A) DESCRIPTION: /desc = "Oligonucleotide"

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 24: ACCACCATGG AGAGCAAAGC CCTG 24

(2) INFORMATION FOR SEQ ID NO: 25: (i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 25 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: other nucleic acid

(A) DESCRIPTION: /desc = "Oligonucleotide"

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 25: CCAGTTTCAG CCTGACTTCT TATTC 25

(2) INFORMATION FOR SEQ ID NO: 26: (i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

[ii) MOLECULE TYPE: other nucleic acid

(A) DESCRIPTION: /desc = "Oligonucleotide'

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 26: GGCTGTGGAC TCAACGATGT C 21

(2) INFORMATION FOR SEQ ID NO: 27:

(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 22 base pairs (B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc = "Oligonucleotide"

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 27:

CCGGGTGGGT AGGTACATTT TG 22 (2) INFORMATION FOR SEQ ID NO: 28:

(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 25 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear (ii) MOLECULE TYPE: other nucleic acid

(A) DESCRIPTION: /desc = "Oligonucleotide"

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 28: GGGGGTGACT TCCAGCCAGG CTGTG 25 (2) INFORMATION FOR SEQ ID NO: 29:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 25 base pairs

(B) TYPE: nucleic acid (C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: other nucleic acid

(A) DESCRIPTION: /desc = "Oligonucleotide"

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:29: AACTCTGAAA GGCATGCCTG CCCGG 25

(2) INFORMATION FOR SEQ ID NO: 30: (i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 26 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: other nucleic acid

(A) DESCRIPTION: /desc = "Oligonucleotide'

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 30: TGAAGGTCGG AGTCAACGGA TTTGGT 26

(2) INFORMATION FOR SEQ ID NO: 31:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 24 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single (D) TOPOLOGY: linear

(ii) MOLECULE TYPE: other nucleic acid

(A) DESCRIPTION: /desc = "Oligonucleotide"

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 31: CATGTGGGCC ATGAGGTCCA CCAC 24

Claims

WE CLAIM

1 . An isolated polypeptide encoded by a lipase like gene, wherein the polypeptide (a) binds heparin, (b) has homology with human lipoprotein lipase and hepatic lipase, and

(c) comprises a 39 kD catalytic domain of the triacylglycerol lipase family.

2. The isolated polypeptide of claim 1, wherein the 39 kD catalytic domain of the triacylglycerol lipase family has the amino acid sequence of SEQ ID NO: 10.

3. The isolated polypeptide of claim 1, wherein the polypeptide has an amino acid sequence of SEQ ID NO: 8 and an apparent molecular weight of about 55 kD on a 10% SDS- PAGE gel.

4. The isolated polypeptide of claim 1 , wherein the polypeptide has an amino acid sequence of SEQ ID NO: 8 and an apparent molecular weight of about 68 kD on a 10% SDS- PAGE gel.

5. The isolated polypeptide of claims 1-4, wherein the polypeptide has phospholipase A activity.

6. The isolated polypeptide of claim 1, wherein the polypeptide has an amino acid sequence of SEQ ID NO: 6 and an apparent molecular weight of about 40 kD on a 10% SDS- PAGE gel.

7. The isolated polypeptide of claims 1-6, wherein the polypeptide is of human origin.

8. A composition comprising the polypeptide of claims 1-7 and a biologically compatible solution.

9. A pharmaceutical composition comprising the polypeptide of claims 1-7 and a pharmaceutically acceptable carrier.

10. An antigenic fragment of the isolated polypeptide of claims 1-7.

11. An isolated nucleic acid encoding the polypeptide of claims 1-7.

12. The isolated nucleic acid of claim 11 which is cDNA.

13. The isolated nucleic acid of claim 12, wherein the nucleotide sequence is selected from the group consisting of SEQ ID NOS: 5, 7 and 9.

14. The isolated nucleic acid of claim 13 comprising nucleotides 252-1754 of SEQ ID NO: 7.

15. An isolated nucleic acid which hybridizes at high stringency to a nucleic acid having a sequence selected from the group consisting of SEQ ID NOS: 3, 5 and 7.

16. The isolated nucleic acid of claim 15 which comprises 20 nucleotides.

17. The isolated nucleic acid of claim 15 which is an antisense nucleic acid.

18. The isolated nucleic acid of claim 17 wherein the antisense nucleic acid sequence is operably linked to a gene expression regulatory region.

19. The isolated nucleic acid of claim 15 which hybridizes at high stringency to a target selected from the group consisting of nucleotides from 44-79 of SEQ ID NO: 3 and nucleotides from 1036-1065 of SEQ ID NO:5.

20. A composition comprising the nucleic acid of claim 17 and a biologically compatible solution.

21. A pharmaceutical composition comprising the nucleic acid of claim 17 and a pharmaceutically acceptable carrier.

22. A vector comprising the isolated nucleic acid of claims 11-14 operably linked to a regulatory region.

23. The vector of claim 22 wherein the regulatory region is from a heterologous source.

24. The vector of claims 22 or 23 which is a viral vector.

25. The vector of claim 24 which is an adenoviral vector.

26. A recombinant cell comprising the vector of claims 22-25.

27. The recombinant cell of claim 26 wherein the cell is a eukaryotic cell.

28. The recombinant cell of claim 27 wherein the cell is a COS-7 cell.

29. A composition comprising the vector of claims 22-25 and a biologically compatible solution.

30. A pharmaceutical composition comprising the vector of claims 22-25 and a pharmaceutically acceptable carrier.

31. A method for preparing a polypeptide comprising culturing the recombinant cell according to claims 26-28 under conditions permitting the expression of the polypeptide.

32. A polypeptide prepared according to the method of claim 31.

33. An antibody capable of specifically binding to the polypeptide of claims 1-7 or 32.

34. An antibody capable of specifically binding to the polypeptide of claim 5 and neutralizing the phospholipase activity of the polypeptide.

35. The antibody of claims 33 or 34 which is a monoclonal antibody.

36. The antibody of claims 33 or 34 which is a polyclonal antibody.

37. A hybridoma cell which produces the antibody of claim 35.

38. A composition comprising the antibody of claims 33-36 and a biologically compatible solution.

39. A pharmaceutical composition comprising the antibody of claims 33-36 and a pharmaceutically acceptable carrier.

40. A method of screening for agonists or antagonists of LLG activity comprising:

(a) contacting potential agonists or antagonists with LLG and a substrate of LLG, and (b) measuring the ability of the potential agonists or antagonists to enhance or inhibit

LLG activity, wherein the LLG is the polypeptide of claim 1. <P

41. A method for the enzymatic hydrolysis of a phosphatidylcholine ester comprising contacting said phosphatidylcholine ester with the polypeptide of claims 1-5.

42. A method of improving the serum lipid profile of a human or other animal having an undesirable lipid profile comprising administration of an effective amount of the pharmaceutical composition of claim 9 or 21.

43. A method of improving the serum lipid profile of a human or other animal having an undesirable lipid profile comprising administration of an effective amount of the composition of claim 39.

44. The isolated polypeptide of claim 1, wherein the polypeptide is of rabbit origin.

45. The isolated polypeptide of claim 45 which has an amino acid sequence of SEQ ID NO: 12.

46. An isolated nucleic acid which encodes the polypeptide of claim 45.

47. The nucleic acid of claim 47 having a nucleic acid sequence of SEQ ID NO: 1 1.

48. A transgenic mouse expressing the polypeptide of claims 1-5.