WO1998028444A3 - Customized oligonucleotide microchips as multiple biosensors - Google Patents

Customized oligonucleotide microchips as multiple biosensors Download PDF

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Publication number
WO1998028444A3
WO1998028444A3 PCT/US1997/023778 US9723778W WO9828444A3 WO 1998028444 A3 WO1998028444 A3 WO 1998028444A3 US 9723778 W US9723778 W US 9723778W WO 9828444 A3 WO9828444 A3 WO 9828444A3
Authority
WO
WIPO (PCT)
Prior art keywords
biosensors
oligonucleotides
customized
microchip
multiple biosensors
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1997/023778
Other languages
French (fr)
Other versions
WO1998028444A2 (en
Inventor
Andrei Mirzabekov
Dmitry Y Guschin
Valentine Shik
Alexander Fotin
Gennadiy Yershov
Yu Lysov
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University of Chicago
Original Assignee
University of Chicago
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Chicago filed Critical University of Chicago
Priority to AU57160/98A priority Critical patent/AU5716098A/en
Priority to EP97953408A priority patent/EP0951569A2/en
Publication of WO1998028444A2 publication Critical patent/WO1998028444A2/en
Publication of WO1998028444A3 publication Critical patent/WO1998028444A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Biosensors are constructed using customized micromatrices to design a microship. The microchip contains an arrangement of oligonucleotides designed to provide information on oligonucleotide sequences in a sample to be tested by the ability of the oligonucleotides in the nucleic acid sample to hybridize with oligonucleotides on the microchip. Biosensors are used to detect microorganisms in the environment, in biological samples and in food. Biosensors are also used to diagnose genetic defects, to identify polymorphisms, and to monitor quantitative gene expression.
PCT/US1997/023778 1996-12-23 1997-12-19 Customized oligonucleotide microchips as multiple biosensors Ceased WO1998028444A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU57160/98A AU5716098A (en) 1996-12-23 1997-12-19 Customized oligonucleotide microchips as multiple biosensors
EP97953408A EP0951569A2 (en) 1996-12-23 1997-12-19 Customized oligonucleotide microchips as multiple biosensors

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US78002696A 1996-12-23 1996-12-23
US08/780,026 1996-12-23

Publications (2)

Publication Number Publication Date
WO1998028444A2 WO1998028444A2 (en) 1998-07-02
WO1998028444A3 true WO1998028444A3 (en) 1998-12-17

Family

ID=25118334

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1997/023778 Ceased WO1998028444A2 (en) 1996-12-23 1997-12-19 Customized oligonucleotide microchips as multiple biosensors

Country Status (3)

Country Link
EP (1) EP0951569A2 (en)
AU (1) AU5716098A (en)
WO (1) WO1998028444A2 (en)

Cited By (8)

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US6942771B1 (en) 1999-04-21 2005-09-13 Clinical Micro Sensors, Inc. Microfluidic systems in the electrochemical detection of target analytes
US7087148B1 (en) 1998-06-23 2006-08-08 Clinical Micro Sensors, Inc. Binding acceleration techniques for the detection of analytes
US7267939B2 (en) 1997-06-12 2007-09-11 Clinical Micro Sensors, Inc. Detection of analytes using reorganization energy
US7381533B2 (en) 1996-11-05 2008-06-03 Clinical Micro Sensors, Inc. Electrodes linked via oligomers to nucleic acids
US7381525B1 (en) 1997-03-07 2008-06-03 Clinical Micro Sensors, Inc. AC/DC voltage apparatus for detection of nucleic acids
US7393645B2 (en) 1996-11-05 2008-07-01 Clinical Micro Sensors, Inc. Compositions for the electronic detection of analytes utilizing monolayers
US7655129B2 (en) 1998-06-23 2010-02-02 Osmetech Technology Inc. Binding acceleration techniques for the detection of analytes
US9874542B2 (en) 1998-09-17 2018-01-23 Clinical Micro Sensors, Inc. Signal detection techniques for the detection of analytes

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US7582421B2 (en) 1993-11-01 2009-09-01 Nanogen, Inc. Methods for determination of single nucleic acid polymorphisms using a bioelectronic microchip
US5952172A (en) 1993-12-10 1999-09-14 California Institute Of Technology Nucleic acid mediated electron transfer
US5824473A (en) 1993-12-10 1998-10-20 California Institute Of Technology Nucleic acid mediated electron transfer
US6071699A (en) 1996-06-07 2000-06-06 California Institute Of Technology Nucleic acid mediated electron transfer
US7160678B1 (en) 1996-11-05 2007-01-09 Clinical Micro Sensors, Inc. Compositions for the electronic detection of analytes utilizing monolayers
US7045285B1 (en) 1996-11-05 2006-05-16 Clinical Micro Sensors, Inc. Electronic transfer moieties attached to peptide nucleic acids
US6096273A (en) 1996-11-05 2000-08-01 Clinical Micro Sensors Electrodes linked via conductive oligomers to nucleic acids
US6458584B1 (en) * 1996-12-23 2002-10-01 University Of Chicago Customized oligonucleotide microchips that convert multiple genetic information to simple patterns, are portable and reusable
EP1051517A2 (en) 1998-01-27 2000-11-15 Clinical Micro Sensors, Inc. Amplification of nucleic acids with electronic detection
US6686150B1 (en) 1998-01-27 2004-02-03 Clinical Micro Sensors, Inc. Amplification of nucleic acids with electronic detection
WO2000024941A1 (en) 1998-10-27 2000-05-04 Clinical Micro Sensors, Inc. Detection of target analytes using particles and electrodes
US6391937B1 (en) 1998-11-25 2002-05-21 Motorola, Inc. Polyacrylamide hydrogels and hydrogel arrays made from polyacrylamide reactive prepolymers
US20020177135A1 (en) 1999-07-27 2002-11-28 Doung Hau H. Devices and methods for biochip multiplexing
US7312087B2 (en) 2000-01-11 2007-12-25 Clinical Micro Sensors, Inc. Devices and methods for biochip multiplexing
DE60038015T2 (en) * 1999-05-03 2009-02-12 Gen-Probe Inc., San Diego METHOD BASED ON A POLYNUCLEOTIDE MATRIX FOR IDENTIFYING MICROORGANISMS
EP1925678B1 (en) * 1999-05-03 2009-07-22 Gen-Probe Incorporated Polynucleotide matrix-based method of identifying microorganisms
US6821770B1 (en) 1999-05-03 2004-11-23 Gen-Probe Incorporated Polynucleotide matrix-based method of identifying microorganisms
CA2380258C (en) 1999-07-26 2008-07-15 Clinical Micro Sensors, Inc. Sequence determination of nucleic acids using electronic detection
KR100348786B1 (en) * 1999-10-01 2002-08-17 엘지전자주식회사 Method for detecting nucleic acids, detector for nucleic acids and method for producing the same
WO2001036683A2 (en) * 1999-11-16 2001-05-25 Apollo Biotechnology, Inc. Method for rapid and accurate identification of microorganisms
US6878517B1 (en) 1999-12-15 2005-04-12 Congra Grocery Products Company Multispecies food testing and characterization organoleptic properties
JP3871846B2 (en) * 2000-03-10 2007-01-24 日立ソフトウエアエンジニアリング株式会社 Hybridization reaction detection method and detection apparatus
US7205104B2 (en) 2000-03-24 2007-04-17 Eppendorf Array Technologies Sa (Eat) Identification of biological (micro) organisms by detection of their homologous nucleotide sequences on arrays
US7829313B2 (en) 2000-03-24 2010-11-09 Eppendorf Array Technologies Identification and quantification of a plurality of biological (micro)organisms or their components
EP1136566A1 (en) * 2000-03-24 2001-09-26 Facultés Universitaires Notre-Dame de la Paix Method and kit for detection and/or quantification of homologus nucleotide sequences on arrays
US7202026B2 (en) 2000-03-24 2007-04-10 Eppendorf Array Technologies Sa (Eat) Identification of a large number of biological (micro)organisms groups at different levels by their detection on a same array
US7875442B2 (en) 2000-03-24 2011-01-25 Eppendorf Array Technologies Identification and quantification of a plurality of biological (micro)organisms or their components
FR2815043B1 (en) * 2000-10-05 2005-09-16 Skuld Tech RAPID METHOD OF DETECTION OF ORGANISMS IN A SAMPLE
FR2816958B1 (en) * 2000-11-17 2004-11-26 Biomerieux Sa METHOD FOR THE ANALYSIS OF THE GENETIC PREDISPOSITION OF A PATIENT WITH INSULIN-DEPENDENT DIABETES, DEVICE SUITABLE FOR ITS IMPLEMENTATION AND SET OF AMPLIFICATION PRIMERS SUITABLE FOR SUCH A PROCESS
US6960298B2 (en) 2001-12-10 2005-11-01 Nanogen, Inc. Mesoporous permeation layers for use on active electronic matrix devices
FR2833968A1 (en) * 2001-12-20 2003-06-27 Ct Medico Chirurgical De Tronq Thematic biochip, useful e.g. for diagnosis, particularly of breast cancer, and treatment selection, comprises thematic probes and probes for interpretation
FR2833969A1 (en) * 2001-12-20 2003-06-27 Ct Medico Chirurgical De Tronq Biochip that carries high purity molecular probes, useful e.g. for diagnosis, specifically of breast cancer, and for analysis of DNA repair
EP1469067A4 (en) * 2002-01-25 2007-05-23 Olympus Corp Method and apparatus for detecting nucleic acid data
EP1524320A1 (en) * 2003-10-15 2005-04-20 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Rapid determination and quantification of mycoplasma contamination using DNA chip technology
GB0326079D0 (en) 2003-11-07 2003-12-10 Rue De Int Ltd Security device
US7338763B2 (en) 2004-06-02 2008-03-04 Eppendorf Array Technologies S.A. Method and kit for the detection and/or quantification of homologous nucleotide sequences on arrays
EP2302072B1 (en) * 2004-11-18 2012-03-14 Eppendorf Array Technologies S.A. Real time-PCR of targets on a micro-array
DE602005005333T2 (en) 2005-09-13 2008-09-18 Eppendorf Array Technologies S.A. Method for detecting homologous sequences differing by a base on a microarray
CN101336301A (en) * 2006-01-05 2008-12-31 西蒙斯单倍体有限公司 microarray method
EP2277032A4 (en) * 2008-05-09 2011-10-26 Zhiping Liu MOLECULE DETECTION SYSTEM
WO2017100454A1 (en) 2015-12-09 2017-06-15 Intuitive Biosciences, Inc. Automated silver enhancement system

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Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7381533B2 (en) 1996-11-05 2008-06-03 Clinical Micro Sensors, Inc. Electrodes linked via oligomers to nucleic acids
US7393645B2 (en) 1996-11-05 2008-07-01 Clinical Micro Sensors, Inc. Compositions for the electronic detection of analytes utilizing monolayers
US7381525B1 (en) 1997-03-07 2008-06-03 Clinical Micro Sensors, Inc. AC/DC voltage apparatus for detection of nucleic acids
US7514228B2 (en) 1997-06-12 2009-04-07 Clinical Micro Sensors, Inc. Detection of analytes using reorganization energy
US7267939B2 (en) 1997-06-12 2007-09-11 Clinical Micro Sensors, Inc. Detection of analytes using reorganization energy
US7087148B1 (en) 1998-06-23 2006-08-08 Clinical Micro Sensors, Inc. Binding acceleration techniques for the detection of analytes
US7655129B2 (en) 1998-06-23 2010-02-02 Osmetech Technology Inc. Binding acceleration techniques for the detection of analytes
US9874542B2 (en) 1998-09-17 2018-01-23 Clinical Micro Sensors, Inc. Signal detection techniques for the detection of analytes
US6942771B1 (en) 1999-04-21 2005-09-13 Clinical Micro Sensors, Inc. Microfluidic systems in the electrochemical detection of target analytes
US7534331B2 (en) 1999-04-21 2009-05-19 Osmetech Technology Inc. Use of microfluidic systems in the electrochemical detection of target analytes
US8486247B2 (en) 1999-04-21 2013-07-16 Osmetch Technology, Inc. Use of microfluidic systems in the electrochemical detection of target analytes
US9151746B2 (en) 1999-04-21 2015-10-06 Osmetech Technology, Inc. Use of microfluidic systems in the electrochemical detection of target analytes
US9557295B2 (en) 1999-04-21 2017-01-31 Osmetech Technology, Inc. Use of microfluidic systems in the electrochemical detection of target analytes

Also Published As

Publication number Publication date
EP0951569A2 (en) 1999-10-27
WO1998028444A2 (en) 1998-07-02
AU5716098A (en) 1998-07-17

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