WO1998042874A3 - Biomolecular processor - Google Patents

Biomolecular processor Download PDF

Info

Publication number
WO1998042874A3
WO1998042874A3 PCT/US1998/006029 US9806029W WO9842874A3 WO 1998042874 A3 WO1998042874 A3 WO 1998042874A3 US 9806029 W US9806029 W US 9806029W WO 9842874 A3 WO9842874 A3 WO 9842874A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acids
target molecules
blood
sample
reagent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1998/006029
Other languages
French (fr)
Other versions
WO1998042874B1 (en
WO1998042874A2 (en
Inventor
Robert E Fields
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to AT98913175T priority Critical patent/ATE291637T1/en
Priority to EP98913175A priority patent/EP0972080B1/en
Priority to DE69829466T priority patent/DE69829466T2/en
Priority to AU67790/98A priority patent/AU6779098A/en
Publication of WO1998042874A2 publication Critical patent/WO1998042874A2/en
Publication of WO1998042874A3 publication Critical patent/WO1998042874A3/en
Publication of WO1998042874B1 publication Critical patent/WO1998042874B1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/405—Concentrating samples by adsorption or absorption
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Rigid containers without fluid transport within
    • B01L3/5085—Rigid containers without fluid transport within for multiple samples, e.g. microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0858—Side walls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

A process and apparatus for isolating and purifying nucleic acids and other target molecules directly from blood, plasma, urine, cell cultures and the like by totally automated means, without centrifugation, aspiration or vacuum; after mixing and heating a nucleic acid containing sample with lysis reagent in an environmentally isolated compartment, nucleic acids are absorbed onto a binding filter and eluted in a small volume using heated elution reagent; a preferred embodiment purifies nucleic acids and automatically detects target sequences from a sample of fresh blood. Another embodiment purifies target molecules from a multitude of samples held in microtiter plates; test kits for each embodiment include disposable isolation and detection devices and associated reagents.
PCT/US1998/006029 1997-03-24 1998-03-23 Biomolecular processor Ceased WO1998042874A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AT98913175T ATE291637T1 (en) 1997-03-24 1998-03-23 BIOMOLECULAR PROCESSOR
EP98913175A EP0972080B1 (en) 1997-03-24 1998-03-23 Biomolecular processor
DE69829466T DE69829466T2 (en) 1997-03-24 1998-03-23 BIOMOLECULAR PROCESSOR
AU67790/98A AU6779098A (en) 1997-03-24 1998-03-23 Biomolecular processor

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US4123797P 1997-03-24 1997-03-24
US60/041,237 1997-03-24

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US09381603 A-371-Of-International 1999-09-22
US10/243,521 Continuation US20030027203A1 (en) 1997-03-24 2002-09-12 Biomolecular processor

Publications (3)

Publication Number Publication Date
WO1998042874A2 WO1998042874A2 (en) 1998-10-01
WO1998042874A3 true WO1998042874A3 (en) 1998-12-23
WO1998042874B1 WO1998042874B1 (en) 1999-01-28

Family

ID=21915486

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/006029 Ceased WO1998042874A2 (en) 1997-03-24 1998-03-23 Biomolecular processor

Country Status (5)

Country Link
EP (1) EP0972080B1 (en)
AT (1) ATE291637T1 (en)
AU (1) AU6779098A (en)
DE (1) DE69829466T2 (en)
WO (1) WO1998042874A2 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19746874A1 (en) * 1997-10-23 1999-04-29 Qiagen Gmbh Isolation of nucleic acids
ATE399215T1 (en) * 1999-03-11 2008-07-15 Whatman Inc SOLID MEDIUM AND METHOD FOR STORAGE AND RAPID PURIFICATION OF NUCLEIC ACIDS
JP2003505703A (en) * 1999-07-23 2003-02-12 テプネル・メディカル・リミテッド Biomolecule isolation
US7229595B2 (en) 2001-06-15 2007-06-12 Molecular Devices Corporation Filtration column devices and methods of filtering therewith
US7749388B2 (en) 2001-06-15 2010-07-06 Life Technologies Corporation Low volume filtration column devices and methods of filtering therewith
US7556733B2 (en) 2001-06-15 2009-07-07 Mds Analytical Technologies (Us) Inc. Low volume filtration column devices and methods of filtering therewith
FR2827873B1 (en) * 2001-07-24 2003-09-26 Amenagement Urbain & Rural AUTOMATED METHOD FOR DETECTING PATHOGENS IN WATER
EP1412470B1 (en) * 2001-07-24 2010-11-03 Societe D'amenagement Urbain Et Rural Automated process for detecting pathogenic organisms in water
EP1417306A2 (en) * 2001-08-10 2004-05-12 Xantos Biomedicine AG High-throughput dna-isolation and transfection for analysing the function of genes or genetic products
DE20117746U1 (en) * 2001-11-02 2002-04-25 InViTek Gesellschaft für Biotechnik & Biodesign mbH, 13125 Berlin Reaction rooms containing complex storage-stable reagent formulations and test kit for the detection and isolation of pathogenic microbial nucleic acids
WO2017216270A1 (en) 2016-06-15 2017-12-21 Grabmayr Heinrich Single molecule detection or quantification using dna nanotechnology
CN109696346B (en) * 2017-10-20 2023-10-13 莱伯泰科(天津)科技有限公司 Isotope pretreatment device
US11994528B2 (en) * 2019-12-18 2024-05-28 Life Technologies Corporation Systems, methods, and devices for automated nucleic acid and protein isolation
CN113030477B (en) * 2019-12-25 2024-01-26 深圳迈瑞生物医疗电子股份有限公司 A measurement method, measurement device and storage medium for specific protein
WO2021254519A1 (en) * 2020-06-19 2021-12-23 中国疾病预防控制中心病毒病预防控制所 Sample processing and detection apparatus and application thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4775635A (en) * 1985-04-12 1988-10-04 E. I. Du Pont De Nemours And Company Rapid assay processor
EP0487028A2 (en) * 1990-11-22 1992-05-27 Shimadzu Corporation Apparatus for extracting and purifying nucleic acid
US5645723A (en) * 1995-08-04 1997-07-08 Tomy Seiko Co., Ltd. Process and apparatus for the extraction and purification of DNA
WO1997032645A1 (en) * 1996-03-06 1997-09-12 Akzo Nobel N.V. Automated nucleic acid extraction from samples

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4775635A (en) * 1985-04-12 1988-10-04 E. I. Du Pont De Nemours And Company Rapid assay processor
EP0487028A2 (en) * 1990-11-22 1992-05-27 Shimadzu Corporation Apparatus for extracting and purifying nucleic acid
US5645723A (en) * 1995-08-04 1997-07-08 Tomy Seiko Co., Ltd. Process and apparatus for the extraction and purification of DNA
WO1997032645A1 (en) * 1996-03-06 1997-09-12 Akzo Nobel N.V. Automated nucleic acid extraction from samples

Also Published As

Publication number Publication date
DE69829466D1 (en) 2005-04-28
AU6779098A (en) 1998-10-20
EP0972080A2 (en) 2000-01-19
DE69829466T2 (en) 2006-01-26
WO1998042874A2 (en) 1998-10-01
EP0972080B1 (en) 2005-03-23
ATE291637T1 (en) 2005-04-15

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