WO1998045705A3 - Compositions and methods for inhibiting light-induced inactivation of biological reagents - Google Patents

Compositions and methods for inhibiting light-induced inactivation of biological reagents Download PDF

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Publication number
WO1998045705A3
WO1998045705A3 PCT/US1998/006793 US9806793W WO9845705A3 WO 1998045705 A3 WO1998045705 A3 WO 1998045705A3 US 9806793 W US9806793 W US 9806793W WO 9845705 A3 WO9845705 A3 WO 9845705A3
Authority
WO
WIPO (PCT)
Prior art keywords
compositions
methods
biological reagent
biological reagents
inhibiting light
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1998/006793
Other languages
French (fr)
Other versions
WO1998045705A2 (en
Inventor
Kenneth F Beuchler
Paul H Mcpherson
Alfred R Sundquist
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Alere San Diego Inc
Original Assignee
Biosite Diagnostics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biosite Diagnostics Inc filed Critical Biosite Diagnostics Inc
Priority to EP98913406A priority Critical patent/EP0972194B1/en
Priority to DE69816051T priority patent/DE69816051T2/en
Priority to AT98913406T priority patent/ATE244404T1/en
Priority to CA002286183A priority patent/CA2286183C/en
Priority to AU67965/98A priority patent/AU6796598A/en
Priority to JP54300498A priority patent/JP2001519908A/en
Publication of WO1998045705A2 publication Critical patent/WO1998045705A2/en
Publication of WO1998045705A3 publication Critical patent/WO1998045705A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/80—Fluorescent dyes, e.g. rhodamine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/13—Tracers or tags
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/2525—Stabilizing or preserving

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Disclosed is a fluorescent conjugate comprising a biological reagent, a fluorescent molecule, and a means for impeding phototoxic degradation of the biological reagent. The impeding means can be a cross-linking substance having a long molecular distance, whereby the cross-linking substance links the fluorescent molecule and the biological reagent; a quencher of singlet oxygen; a quencher of a free radical; or a combination thereof. Also disclosed is a solution comprising a fluorescent conjugate of a biological reagent and a fluorescent molecule together with an oxygen depleting system.
PCT/US1998/006793 1997-04-09 1998-04-06 Compositions and methods for inhibiting light-induced inactivation of biological reagents Ceased WO1998045705A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP98913406A EP0972194B1 (en) 1997-04-09 1998-04-06 Compositions and methods for inhibiting light-induced inactivation of biological reagents
DE69816051T DE69816051T2 (en) 1997-04-09 1998-04-06 COMPOSITIONS AND METHODS FOR INHIBITING THE LIGHT-INDUCED INACTIVATION OF BIOLOGICAL REAGENTS
AT98913406T ATE244404T1 (en) 1997-04-09 1998-04-06 COMPOSITIONS AND METHODS FOR INHIBITING LIGHT-INDUCED INACTIVATION OF BIOLOGICAL REAGENTS
CA002286183A CA2286183C (en) 1997-04-09 1998-04-06 Compositions and methods for inhibiting light-induced inactivation of biological reagents
AU67965/98A AU6796598A (en) 1997-04-09 1998-04-06 Novel compositions and methods for inhibiting light-induced inactivation of biological reagents
JP54300498A JP2001519908A (en) 1997-04-09 1998-04-06 Novel compositions and methods for inhibiting light-induced inactivation of biological reagents

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/837,309 US6544797B1 (en) 1997-04-09 1997-04-09 Compositions and methods for inhibiting light-induced inactivation of biological reagents
US08/837,309 1997-04-09

Publications (2)

Publication Number Publication Date
WO1998045705A2 WO1998045705A2 (en) 1998-10-15
WO1998045705A3 true WO1998045705A3 (en) 1998-12-30

Family

ID=25274130

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/006793 Ceased WO1998045705A2 (en) 1997-04-09 1998-04-06 Compositions and methods for inhibiting light-induced inactivation of biological reagents

Country Status (8)

Country Link
US (2) US6544797B1 (en)
EP (1) EP0972194B1 (en)
JP (1) JP2001519908A (en)
AT (1) ATE244404T1 (en)
AU (1) AU6796598A (en)
CA (1) CA2286183C (en)
DE (1) DE69816051T2 (en)
WO (1) WO1998045705A2 (en)

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US11008359B2 (en) 2002-08-23 2021-05-18 Illumina Cambridge Limited Labelled nucleotides
US20040241742A1 (en) * 2003-05-30 2004-12-02 Peck Bill J. Ligand array processing methods that include a low surface tension fluid deposition step and compositions for practicing the same
US20040241880A1 (en) * 2003-05-30 2004-12-02 Leproust Eric M. Ligand array assays having reduced fluorescent dye degradation and compositions for practicing the same
GB0424294D0 (en) * 2004-11-03 2004-12-01 Elam T Ltd Buffer layer
WO2006064199A1 (en) * 2004-12-13 2006-06-22 Solexa Limited Improved method of nucleotide detection
GB0427236D0 (en) * 2004-12-13 2005-01-12 Solexa Ltd Improved method of nucleotide detection
US7998717B2 (en) 2005-12-02 2011-08-16 Pacific Biosciences Of California, Inc. Mitigation of photodamage in analytical reactions
EP2049902A4 (en) * 2006-07-28 2010-09-01 Biosite Inc Devices and methods for performing receptor binding assays using magnetic particles
US20090043109A1 (en) * 2007-08-07 2009-02-12 Spangler Brenda D Asymmetric one- and two-photon fluorophores for simultaneous detection of multiple analytes using a common excitation source
US20090062386A1 (en) * 2007-08-30 2009-03-05 Clean Earth Technologies, Llc Simulants of Toxants for Training and Testing
US20090057622A1 (en) * 2007-08-31 2009-03-05 Clean Earth Technologies, Llc Simulant of Radiological Contamination
WO2009100382A1 (en) * 2008-02-07 2009-08-13 Pacific Biosciences Of California, Inc. Cis reactive oxygen quenchers integrated into linkers
US7968702B2 (en) * 2008-03-13 2011-06-28 Pacific Biosciences Of California, Inc. Labeled reactants and their uses
WO2010059880A2 (en) * 2008-11-19 2010-05-27 Pacific Biosciences Of California, Inc. Photo-induced damage mitigating agents and preparation and methods of use thereof
DE102009010563A1 (en) 2009-02-16 2010-08-26 Matthias W. Engel Device for the detection of analytes in body fluids
GB0913258D0 (en) 2009-07-29 2009-09-02 Dynex Technologies Inc Reagent dispenser
US9523701B2 (en) 2009-07-29 2016-12-20 Dynex Technologies, Inc. Sample plate systems and methods
WO2012021733A2 (en) * 2010-08-12 2012-02-16 Pacific Biosciences Of California, Inc. Photodamage mitigation compounds and systems
US9777320B2 (en) 2010-08-25 2017-10-03 Pacific Biosciences Of California, Inc. Molecular adaptors for dye conjugates
US8486717B2 (en) 2011-01-18 2013-07-16 Symbolics, Llc Lateral flow assays using two dimensional features
US8835000B2 (en) * 2011-12-23 2014-09-16 General Electric Company High-density fluorescent dye clusters
DE102012102082B3 (en) * 2012-03-13 2013-03-21 Thyssenkrupp Rasselstein Gmbh A method of treating a metal-coated steel strip or sheet with an aftertreatment agent and a steel strip or sheet provided with a metal coating.
US9315864B2 (en) 2012-05-18 2016-04-19 Pacific Biosciences Of California, Inc. Heteroarylcyanine dyes with sulfonic acid substituents
EP2850086B1 (en) 2012-05-18 2023-07-05 Pacific Biosciences Of California, Inc. Heteroarylcyanine dyes
US9874556B2 (en) 2012-07-18 2018-01-23 Symbolics, Llc Lateral flow assays using two dimensional features
US20140072959A1 (en) 2012-09-12 2014-03-13 Force Diagnostics, Inc. Rapid tests for insurance underwriting
EP2923204A1 (en) * 2012-10-31 2015-09-30 Astute Medical, Inc. Quantitative lateral flow assay
CN105102980B (en) 2013-02-26 2017-11-03 阿斯图特医药公司 Lateral flow assays with strip keeper
WO2014139596A1 (en) 2013-03-15 2014-09-18 Illumina Cambridge Limited Modified nucleosides or nucleotides
CN108051590B (en) 2013-09-13 2020-12-11 Symbolics有限责任公司 Lateral tomography detection using 2D assay and control signal readout modes
JP6286966B2 (en) * 2013-09-18 2018-03-07 コニカミノルタ株式会社 RESIN PARTICLE FOR FLUORESCENT LABELING, PROCESS FOR PRODUCING THE SAME, AND FLUORESCENT LABELING AGENT FOR IMMUNO STAINING CONTAINING THE SAME
BR112017021305A2 (en) 2015-04-06 2018-06-26 Bludiagnostics Inc lateral flow test device, and method and kit for quantitative detection of an analyte in a saliva sample
EP3676612A4 (en) * 2017-08-31 2021-05-26 Agilent Technologies, Inc. COMPOSITION FOR THE STABILIZATION OF ANTIBODY FORMULATIONS CONTAINING PERCP
WO2019084458A1 (en) * 2017-10-26 2019-05-02 The University Of Houston System Highly-specific assays
US11761040B2 (en) 2020-04-20 2023-09-19 Mgi Tech Co., Ltd. DNA protection agent in DNA imaging buffer

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WO1991018006A1 (en) * 1990-05-15 1991-11-28 Diatron Corporation Fluorescent porphyrin, and fluorescent phthalocyanine - polyethylene glycol, polyol, and saccharide derivatives as fluorescent probes
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WO1995008772A1 (en) * 1993-09-24 1995-03-30 Biosite Diagnostics Incorporated Fluorescence energy transfer and intramolecular energy transfer in particles using novel compounds
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WO1991018006A1 (en) * 1990-05-15 1991-11-28 Diatron Corporation Fluorescent porphyrin, and fluorescent phthalocyanine - polyethylene glycol, polyol, and saccharide derivatives as fluorescent probes
WO1992018868A1 (en) * 1991-04-10 1992-10-29 Biosite Diagnostics Incorporated Crosstalk inhibitors and their uses
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Also Published As

Publication number Publication date
DE69816051D1 (en) 2003-08-07
JP2001519908A (en) 2001-10-23
AU6796598A (en) 1998-10-30
US20030129642A1 (en) 2003-07-10
EP0972194B1 (en) 2003-07-02
US6544797B1 (en) 2003-04-08
DE69816051T2 (en) 2004-05-19
CA2286183A1 (en) 1998-10-15
EP0972194A2 (en) 2000-01-19
WO1998045705A2 (en) 1998-10-15
ATE244404T1 (en) 2003-07-15
US7588908B2 (en) 2009-09-15
CA2286183C (en) 2008-11-18

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