WO1998058942A1 - Non-sulfonated cyanine dyes for labeling nucleosides and nucleotides - Google Patents

Non-sulfonated cyanine dyes for labeling nucleosides and nucleotides Download PDF

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Publication number
WO1998058942A1
WO1998058942A1 PCT/US1998/012593 US9812593W WO9858942A1 WO 1998058942 A1 WO1998058942 A1 WO 1998058942A1 US 9812593 W US9812593 W US 9812593W WO 9858942 A1 WO9858942 A1 WO 9858942A1
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group
compound
dye
integer
lower alkyl
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Charles K. Brush
Ned D. Reimer
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Global Life Sciences Solutions USA LLC
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Amersham Pharmacia Biotech Inc
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Priority to JP50473699A priority Critical patent/JP2002507203A/en
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Priority to CA002294979A priority patent/CA2294979A1/en
Priority to DE69815342T priority patent/DE69815342T2/en
Priority to EP98930319A priority patent/EP0989990B1/en
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom

Definitions

  • Cyanine dyes have been described in the literature for many years 1 ' 2 , mainly for photographic purposes. In recent years, researchers have taken advantage of the excellent fluorescent properties of the carbocyanines to label biological molecules. Initial efforts were thwarted by the high background and/or quenching of fluorescence observed when the dyes were conjugated to proteins. The hydrophobic nature of the dyes caused them to aggregate in aqueous media or on the hydrophobic domains of proteins. Thus, the dyes, as described m the early literature, were not suitable for labeling.
  • Waggoner, et, aj ⁇ . 3,4 disclosed the use of sulfonated derivatives of carbocyanines to label biological molecules.
  • the sulfonate group was found to be effective at preventing aggregation, because of the repulsion of the negative charges between molecules.
  • US 5,556,959 discloses the use of carbocyanine phosphoramidites to label synthetic oligonucleotides . Due to the constraints of the automated systems used for DNA synthesis, the amidites had to be soluble in aprotic organic solvents. Sulfonated carbocyanines are insoluble in the solvents best suited for oligonucleotide synthesis. Therefore, the dye amidites described m US 5,556,959 lacked the sulfonate groups. Experiments showed that the amidites were soluble m the appropriate solvents, such as acetonit ⁇ le and dichloromethane, and labeled the oligonucleotides in high yield. The dye amidites and intermediates are easily and efficiently synthesized and purified.
  • NTPs labeled with reporter groups have been m use for many years 5 c NTPs labeled with sulfonated carbocyanines have been reported m the scientific literature 73 , and are commercially availaole 713 .
  • synthesis of sulfonated cyamnes is a difficult procedure, and the purity of the dye intermediates used m labeling is variable. The recommended shelf-life is short. Reagents for labeling are therefore expensive, as are the labeled NTPs derived from them. Needed m the art of molecular biology is a nonsulfonated cyanine dye attached to a nucleotide or nucleoside .
  • R 3 -0- Sug wherein R 1 is selected from the group consisting of alkyl, aralkyl , and substituted alkyl.
  • Preferable R substitutions include, but are not limited to, OR , COOR , NR 2 R 2 , SR 2 , most preferably where R 2 is H, a removable protecting group, or a lower alkyl group.
  • R 3 is H, PO, - , P 2 0 6 3 ; P 3 0 9 4 , ⁇ -thio phosphates, such as PS0 2 2 ; P 2 S0 5 J ; P 3 S0 8 4 , and BH 3 phosphates, such as P(BH 3 )0 2 2 , P 2 (BH 3 )0 5 J , P 3 (BH 3 )0 8 ⁇ 4 .
  • R 4 is selected from the group consisting of H, lower alkyl, acyl, and (CH 2 ) p C00 (CH 2 ) q CH 3 , wherein p is an integer from 0 to 4 and q is an integer from 0 to .
  • R 5 is selected from the group consisting of H, lower alkyl, acyl, and (CH 2 ) p C00 (CH 2 ) q CH 3 wherein p is an integer from O to 4 and q is an integer from O to 4.
  • R 4 or R 5 may also be 5,6; 6,7; or 7 , 8 -butadienyl (thus forming a second fused aromatic ring) .
  • r is 1, 2, or 3 and X and Y are O, S, C(R 6 ) 2 , N(R 6 ) (wherein R 6 is preferably CH 3 or a lower alkyl) .
  • R 3 -0 - Sugar - Base is a nucleotide or nucleoside .
  • Fig. 1 is a schematic diagram of the synthesis of a cyanme dye- linked nucleoside originating with an amidite synthesis intermediate.
  • Fig. 2 is a schematic diagram of the synthesis of a cyanme dye-l ked nucleotide.
  • Fig. 3 is a diagram of indodicarbocyanine (IDC)- dCTP.
  • Fig. 4 is a diagram of alternative linkers.
  • Fig. 5 is a diagram of the general formula of the present invention.
  • the present invention is a chemical compound of the general formula
  • R 1 is selected from the group consisting of alkyl, aralkyl , and substituted alkyl chains.
  • R is (CH 2 ) 3 OH.
  • Other preferred R groups are (CH 2 ) 5 COOH, (CH 2 ) 3 NH 2 , and C 2 H 5 .
  • R 3 is either H, P0 3 " 2 , P 2 0 6 ⁇ 3 , P 3 0 9 ⁇ 4 , ⁇ -thio phosphates, such as PS0 2 " 2 , P 2 S0 5 “ 3 , P 3 S0 8 - 4 , and ⁇ BH 3 " phosphates, such as P(BH 3 )0 2 - 2 , P 2 (BH 3 )0 5 "3 , or P 3 (BH 3 )0 8 - 4 .
  • R 4 is selected from the group consisting of H, lower alkyl, acyl, and ( CH 2 ) p COO ( CH 2 ) q CH 3 , wherein p is an integer from 0 to 4 and q is an integer from 0 to 4.
  • R s is selected from the group consisting of H, lower alkyl, acyl, and ( CH 2 ) p C00 ( CH 2 ) q CH 3 wherein p is an integer from O to 4 and q is an integer from 0 to 4.
  • R 4 or R 5 may also be 5,6; 6,7; or 7,8- butadienyl (thus forming a second fused aromatic ring) .
  • butadienyl compounds are disclosed m 08/799,593 filed February 10, 1997, by Brush and Anderson, which hereby is incorporated by reference.
  • Linker is a combination of carbon, oxygen, nitrogen, and/or sulfur atoms in a chain that connects the dye through Nl to a position on the base.
  • the linker may contain amide, ester, urea, thiourea, amine, ether, sulfide or disulfide bonds.
  • the position on the base may be C 5 or C 6 of uracil, C 6 of thymine, N 4 , C 5 , or C 6 of cytos e, N 2 , N 7 , or C 8 of guanine, N 2 , C 7 , or C 8 of 7-deazaguanme , C 8 of hypoxanthine , C 7 or C 8 of 7-deazahypoxanthme, N 6 or C 8 of adenme, or N 6 , C , or C 8 of 7-deazaaden ⁇ ne .
  • Preferable linkers are listed below in the Examples (for example, propyl-0-P0 2 -0-hexyl , propyl-0 2 C-ethyl -CO, propyl-0 2 C-ethyl-CONH-hexyl , and propyl-0 2 C-ethyl -CONH-propynyl ) and m Fig. 4.
  • Preferable linkers are between 3 and 25 atoms m length.
  • Base, sugar and R 3 combine to form nucleotides and nucleosides known to one of skill m the art.
  • Base may be uracil, thymine, cytosme, guanine, 7-deazaguanme, hypoxanthine, 7-deazahypoxanthine , adenme, or 7-deazaadenme, 2 , 6-d ⁇ ammopurme or other nitrogen-heterocycle bases, such as those described in reference 8 and references therein.
  • “Sugar” may be ribosyl , 2 ' -deoxyribosyl , 3 ' -deoxyribosyl , or 2 ' , 3 ' -dideoxyribosyl or 2 ' -oxabutyl , the sugar being preferably attached at Nl to the pyrimidines, and N9 to the purmes and deazapurmes .
  • “R 3 -0-Sugar-Base” indicates that the R 3 group is preferably attached to the 5' oxygen of the sugar. In the case of the 2 -oxabutyl "sugar,” there is no 5' oxygen and the R 3 group would be attached to the 4' oxygen.
  • the aromatic quaternary ammonium salt is prepared by alkyiation of a 2 -methyl dolenme, benzoxazole, or benzthiazole , or related benzo derivative.
  • the alkylating agent contains a (protected) functional group which may be further derivatized.
  • Two molecules of the resulting quaternary salt are condensed with one molecule of a protected unsaturated dialdehyde to yield a symmetrical cyanine .
  • one molecule is condensed with a di-anil of an unsaturated dialdehyde.
  • the product is then condensed with a different aromatic quaternary ammonium salt to give an unsymmet ⁇ cal cyanme.
  • the present invention is also a method of labelling a nucleic acid.
  • a nucleic acid Preferably, one would incorporate the compound described above into the nucleic acid chain in the same manner that one incorporates other nucleotides. In a most preferaole form of the invention, one would then determine the nucleic acid se ⁇ uence of the labelled nucleic acid molecule.
  • NTP signifies a nucleoside, or nucleoside mono-, di-, or triphosphate, bound through an ammo group to the linker and dye. All the synthesized examples are triphosphates , as they are the most difficult, but also most useful, compounds to prepare. The following abbreviations are used: TABLE 1
  • IMC-ddCTP A study using visible spectroscopy was done to determine whether the hydrophobic dyes aggregate in aqueous solution. The greater the shorter wavelength shoulder, the more aggregation is occurring.
  • concentration of indot ⁇ carbocyanme-ddATP one of the most hydrophobic dye-nucleoside conjugates, was varied over a range from 0.6 to 80 ⁇ M.
  • the reaction was stirred overnight, and then treated with 10 mL of 0.35 M iodine in a mixture of pyridine, water, and collidine.
  • the solution was diluted with dichloromethane and extracted with aqueous bicarbonate and brine. The solution was dried and evaporated to leave the fully protected phosphotriester .
  • the aminohexyl -derivatized dye above was dissolved 500 ⁇ L of 0.1 M sodium borate, pH 9.2. To this was added 6-chloropurme-9- (1 ' - ⁇ -deoxyr ⁇ bos ⁇ de-5 ' -O- triphosphate) . The reaction was stirred overnight at 60°C, after which time HPLC (C-18, 0-70% acetonitrile/TEAA) analysis showed a high percentage of the product. The mam peak was isolated by prep C-18
  • the material was compared with Cy5-29 ,M -dATP , prepared by reaction of commercially obtained Cy5-29TM-OSu (NHS ester) with N 6 -aminohexyl -dATP .
  • the UV/visible spectra were identical, and the automated sequencing results obtained on ALFexpress (Pharmacia Biotech) were comparable.
  • the sequencing results demonstrate that there is no difference m the reaction of the unsulfonated material described here, and the Cy5TM-29-labeled material, which bears two sulfonate groups .
  • the mono MMTr intermediate for the preparation of the amidite (1 g, 0.00128 mol), was dissolved m 10 mL of pyridme and treated with 0.384 g (0.004 mol) succmic anhydride and 0.11 g 4-d ⁇ methylammopyr ⁇ dme (0.0058 mol) .
  • the reaction was stirred for 4 hours at ambient temperature. Progress was monitored by C-18 HPLC on a 3 ⁇ m column at 80% acetonitrile/TEAA, isocratic, detected at 648 nm. After the addition of 1 mL of water, the reaction was evaporated to dryness . The residue was dissolved m dichloromethane and was extracted with aqueous bicarbonate and brine.
  • the material from the previous reaction was dissolved in 30 mL of 80% acetic acid m water. After five hours at ambient temperature, the detritylation was complete, with no hydrolysis of the succmate ester. Progress was monitored by C-18 HPLC on a 3 ⁇ m column at 50% acetonitrile/TEAA, isocratic for 1 minute, then to 100% acetonitrile in 10 minutes, detected at 648 nm. The solution was evaporated and the residue dissolved m dichloromethane, extracted with aqueous bicarbonate three times, and brine. The solution was dried and evaporated to a blue powder.
  • the dry solid was dissolved in 10 mL of dry dichloromethane, followed by 0.5 mL pyrid e and 0.81 g ( ⁇ 3 eq) of 0- tr fluoroacetyl -N-hydroxysuccmimide .
  • the reaction monitored by C-18 HPLC on a 3 urn column at 50% acetonitrile/TEAA, isocratic for 1 minute, then to 100% acetonitrile m 10 minutes, detected at 648 nm, was over 5 minutes.
  • Dichloromethane was added to 30 mL and the solution was extracted with water three times, dried, and evaporated.
  • N 4 - (6 -Aminohexyl ) -dCTP was dissolved in 0.3 mL of 0.1 M sodium carbonate, pH 9.4. To this was added 200 ⁇ L of
  • the material was compared with Cy5-29TM-dCTP, prepared by reaction of commercially obtained Cy5-29 ,M -OSu (NHS ester) with N 4 -ammonexyl-dCTP .
  • the UV/visible spectra were identical, and the sequencing results obtained on ALFexpress (Pharmacia Biotech) were comparable.
  • the sequencing results demonstrate that there is no difference in the reaction of the unsulfonated material described here, and the Cy5TM-29-labeled material, which bears two sulfonate groups .
  • UV/visible spectrum showed the expected peaks at 646 nm for the dye and 276 nm for an N -der ⁇ vat ⁇ zed cytosme. Yield: 59% of material absorbing at 646 nm, by HPLC analysis. The material was incorporated by a DNA polymerase m a standard sequencing assay, terminating chain extension.
  • 1, 1"-B ⁇ s- (3"- (1 -hydroxypropyl) )-3,3,3',3'- tetramethyl- indotricarbocyanine) (0.5 g) was co-evaporated twice with dry pyridme, dissolved m 10 mL of pyrid e and treated with 65 mg (1 eq . ) of succmic anhydride and 0.055 g 4 -dimethylammopyridme . The reaction was stirred for 4 hours at ambient temperature. Progress was monitored by C-18 HPLC on a 4 um column at 60% acetonitrile/TEAA, isocratic. After the addition of 1 mL of water, the reaction was evaporated to dryness.
  • the appropriate peaks were pooled and desalted on a C-18 cartridge.
  • the UV/visible spectrum showed the expected peaks at 744 nm for the dye and 274 nm for an N 4 -derivat ⁇ zed cytosme. Yield: 1.5 ⁇ mol .
  • the material was incorporated by a DNA polymerase in a standard sequencing assay, terminating chain extension.
  • the appropriate peaks were pooled, the acetonitrile evaporated, and the product stored in ammonium pnospnate solution.
  • the UV/visible spectrum snowed tne expected peaks at 746 nm for the dye and 271 nm for an N 6 -suD ⁇ t ⁇ tuted adenme. Yield: 48 nmol .
  • the mono MMTr intermediate from the preparation of the IMC amidite (0.2 g) was co-evaporated twice with dry pyridme, dissolved m 2 mL of pyrid e, and treated with 0.077 g succmic anhydride and 0.022 g 4-d ⁇ methylammopyr ⁇ dme .
  • N 4 - (6-Ammonexyl ) -dCTP '1 mg) prepared from diammohexane and 2 ' -deoxy-CTP by bisulfite catalysis, was dissolved in 0.1 M aqueous carbonate buffer, pH 9.5.
  • (1- (3 - (1 '" -propyloxysuccinic acid, N-hydroxysuccmimide ester) )-l'-(3"-(l"- hydroxypropyl) )-3,3,3',3'- tetramethyl- indomonocarbocyanine) (1 mg) was added in 50 ⁇ L each of DMF and water. The pH was adjusted to 9.2.
  • the mono-acetyl derivative (175 mg) was dried by co- evaporation three times with dry acetonitrile and dissolved m 1 mL of dry pyridme. Succmic anhydride and DMAP were added and the reaction proceeded for 2 hours. The reaction was quenched with 1 mL water and the product purified by C18 prep HPLC on a 25 x 100 mm Novapak cartridge using a 0-80% acetonitrile/TEAA gradient. Yield: 22 mg.
  • the succ ate derivative (22 mg) was dried by co- evaporation two times with dry pyridme and dissolved 1 mL of dry dichloromethane with 50 ⁇ L of dry pyrid e. O-Trifluoroacetyl -N-hydroxysuccmimide (100 mg) was added. After 5 minutes the reaction was diluted with 5 mL dichloromethane and extracted twice with water. The dichloromethane was dried and evaporated to yield 20 mg of activated ester.

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Abstract

A chemical compound of formula: (I), wherein R1 is selected from the group consisting of alkyl, aralkyl, and substituted alkyl groups; R3 is selected from the group consisting of H, PO¿3?-2; P2O6-3; P3O9-4, and α-thio phosphates (PSO¿2?-2; P2SO5-3; P3SO8-4); and αBH¿3?- phosphates (P(BH¿3?)O2?-2, P¿2(BH3)O5-3, P3(BH3)O8-4); R4 is selected from the group consisting of H, lower alkyl, acyl, (CH¿2?)pCOO(CH2)qCH3 wherein p is an integer from 0 to 4 and q is an integer from 0 to 4, and 5,6; 6,7; or 7,8- butadienyl, R?5¿ is selected from the group consisting of H, lower alkyl, acyl, (CH¿2?)pCOO(CH2)qCH3 wherein p is an integer from 0 to 4 and q is an integer from 0 to 4 and 5,6; 6,7; or 7,8- butadienyl; r is 1, 2 or 3 to form a second fused aromatic; X or Y are selected from the group consisting of O, S, C(R?6)¿2, or N(R6), wherein R6 is preferably CH¿3? or a lower alkyl; and R?3¿-0 - Sugar-Base is a nucleoside or nucleotide is disclosed.

Description

NON-SULFONATED CYANINE DYES FOR LABELING NUCLEUSIDES AND NUCLEOTIDES
BACKGROUND OF THE INVENTION
Cyanine dyes have been described in the literature for many years1'2, mainly for photographic purposes. In recent years, researchers have taken advantage of the excellent fluorescent properties of the carbocyanines to label biological molecules. Initial efforts were thwarted by the high background and/or quenching of fluorescence observed when the dyes were conjugated to proteins. The hydrophobic nature of the dyes caused them to aggregate in aqueous media or on the hydrophobic domains of proteins. Thus, the dyes, as described m the early literature, were not suitable for labeling.
Waggoner, et, aj^.3,4 disclosed the use of sulfonated derivatives of carbocyanines to label biological molecules. The sulfonate group was found to be effective at preventing aggregation, because of the repulsion of the negative charges between molecules. In some of the cited Waggoner disclosures, the importance of the sulfonate groups to the novelty and efficacy of the dye derivatives, which included nucleic acids, was emphasized.
US 5,556,959 discloses the use of carbocyanine phosphoramidites to label synthetic oligonucleotides . Due to the constraints of the automated systems used for DNA synthesis, the amidites had to be soluble in aprotic organic solvents. Sulfonated carbocyanines are insoluble in the solvents best suited for oligonucleotide synthesis. Therefore, the dye amidites described m US 5,556,959 lacked the sulfonate groups. Experiments showed that the amidites were soluble m the appropriate solvents, such as acetonitπle and dichloromethane, and labeled the oligonucleotides in high yield. The dye amidites and intermediates are easily and efficiently synthesized and purified. Nucleos de triphosphates (NTPs) labeled with reporter groups have been m use for many years5 c NTPs labeled with sulfonated carbocyanines have been reported m the scientific literature73, and are commercially availaole713. However, synthesis of sulfonated cyamnes is a difficult procedure, and the purity of the dye intermediates used m labeling is variable. The recommended shelf-life is short. Reagents for labeling are therefore expensive, as are the labeled NTPs derived from them. Needed m the art of molecular biology is a nonsulfonated cyanine dye attached to a nucleotide or nucleoside .
BRIEF SUMMARY OF THE INVENTION The present invention is a chemical compound of the following formula:
R3-0- Sug
Figure imgf000004_0001
wherein R1 is selected from the group consisting of alkyl, aralkyl , and substituted alkyl. Preferable R substitutions include, but are not limited to, OR , COOR , NR2R2, SR2, most preferably where R2 is H, a removable protecting group, or a lower alkyl group. R3 is H, PO, - , P206 3; P309 4, α-thio phosphates, such as PS02 2 ; P2S05 J ; P3S08 4, and BH3 phosphates, such as P(BH3)02 2, P2(BH3)05 J, P3(BH3)08 ~4. R4 is selected from the group consisting of H, lower alkyl, acyl, and (CH2) pC00 (CH2) qCH3, wherein p is an integer from 0 to 4 and q is an integer from 0 to . R5 is selected from the group consisting of H, lower alkyl, acyl, and (CH2) pC00 (CH2) qCH3 wherein p is an integer from O to 4 and q is an integer from O to 4. R4 or R5 may also be 5,6; 6,7; or 7 , 8 -butadienyl (thus forming a second fused aromatic ring) . r is 1, 2, or 3 and X and Y are O, S, C(R6)2, N(R6) (wherein R6 is preferably CH3 or a lower alkyl) . R3-0 - Sugar - Base is a nucleotide or nucleoside .
It is an object of the present invention to provide a nucleotide or nucleoside attached to a nonsulfonated cyanme dye .
It is another ob ect of this present invention to provide a nucleoside or nucleotide attached to a fluorescent label . Other objects, advantages, and features of the present invention will become apparent after one has examined the specification, claims, and drawings of the present invention.
BRIEF DESCRIPTION OF THE SEVERAL VIEWS OF THE DRAWINGS
Fig. 1 is a schematic diagram of the synthesis of a cyanme dye- linked nucleoside originating with an amidite synthesis intermediate.
Fig. 2 is a schematic diagram of the synthesis of a cyanme dye-l ked nucleotide. Fig. 3 is a diagram of indodicarbocyanine (IDC)- dCTP.
Fig. 4 is a diagram of alternative linkers. Fig. 5 is a diagram of the general formula of the present invention.
DETAILED DESCRIPTION OF THE INVENTION
The present invention is a chemical compound of the general formula
R3-0- Sug
Figure imgf000006_0001
R1 is selected from the group consisting of alkyl, aralkyl , and substituted alkyl chains. Preferably, the substitutions are OR2, COOR2, NR2R2 , SR2 , where R2 is preferably H, a removable protecting group such as tπtyl or acetyl, or a lower alkyl group (n= 1-4) . In the examples below, we describe a compound of the present invention in which R is (CH2)3OH. Other preferred R groups are (CH2)5COOH, (CH2)3NH2, and C2H5.
R3 is either H, P03"2, P206 ~3, P309 ~4, α-thio phosphates, such as PS02"2, P2S05"3, P3S08-4, and αBH3" phosphates, such as P(BH3)02-2, P2(BH3)05 "3, or P3(BH3)08-4. R4 is selected from the group consisting of H, lower alkyl, acyl, and ( CH2 ) pCOO ( CH2 ) qCH3 , wherein p is an integer from 0 to 4 and q is an integer from 0 to 4. Rs is selected from the group consisting of H, lower alkyl, acyl, and ( CH2 ) pC00 ( CH2 ) qCH3 wherein p is an integer from O to 4 and q is an integer from 0 to 4. R4 or R5 may also be 5,6; 6,7; or 7,8- butadienyl (thus forming a second fused aromatic ring) . r is 1, 2, or 3 and X and Y are 0, S, C(R6)2, NR6, wherein R6 is preferably CH3 or a lower alkyl (n=l-4), such as CH2CH3.
The butadienyl compounds are disclosed m 08/799,593 filed February 10, 1997, by Brush and Anderson, which hereby is incorporated by reference.
"Linker" is a combination of carbon, oxygen, nitrogen, and/or sulfur atoms in a chain that connects the dye through Nl to a position on the base. The linker may contain amide, ester, urea, thiourea, amine, ether, sulfide or disulfide bonds. The position on the base may be C5 or C6 of uracil, C6 of thymine, N4, C5, or C6 of cytos e, N2, N7, or C8 of guanine, N2 , C7, or C8 of 7-deazaguanme , C8 of hypoxanthine , C7 or C8 of 7-deazahypoxanthme, N6 or C8 of adenme, or N6, C , or C8 of 7-deazaadenιne . Preferable linkers are listed below in the Examples (for example, propyl-0-P02-0-hexyl , propyl-02C-ethyl -CO, propyl-02C-ethyl-CONH-hexyl , and propyl-02C-ethyl -CONH-propynyl ) and m Fig. 4. Preferable linkers are between 3 and 25 atoms m length.
Base, sugar and R3 combine to form nucleotides and nucleosides known to one of skill m the art.
"Base" may be uracil, thymine, cytosme, guanine, 7-deazaguanme, hypoxanthine, 7-deazahypoxanthine , adenme, or 7-deazaadenme, 2 , 6-dιammopurme or other nitrogen-heterocycle bases, such as those described in reference 8 and references therein.
"Sugar" may be ribosyl , 2 ' -deoxyribosyl , 3 ' -deoxyribosyl , or 2 ' , 3 ' -dideoxyribosyl or 2 ' -oxabutyl , the sugar being preferably attached at Nl to the pyrimidines, and N9 to the purmes and deazapurmes . "R3-0-Sugar-Base" indicates that the R3 group is preferably attached to the 5' oxygen of the sugar. In the case of the 2 -oxabutyl "sugar," there is no 5' oxygen and the R3 group would be attached to the 4' oxygen.
The Examples below disclose preferred methods of synthesis of the compound of the present invention. In general, the synthesis of the compounds may oe described as follows: The aromatic quaternary ammonium salt is prepared by alkyiation of a 2 -methyl dolenme, benzoxazole, or benzthiazole , or related benzo derivative. The alkylating agent contains a (protected) functional group which may be further derivatized. Two molecules of the resulting quaternary salt are condensed with one molecule of a protected unsaturated dialdehyde to yield a symmetrical cyanine . Alternatively, one molecule is condensed with a di-anil of an unsaturated dialdehyde. The product is then condensed with a different aromatic quaternary ammonium salt to give an unsymmetπcal cyanme.
The functional group on tne alkylatmg agent s then deprotecteά [ if necessary; and derivatized tt yield ar. activated group which is capable of reacting with a group on the nucleoside triphosphate. Many methods are known to the art which can be adapted to accomplish this.
The present invention is also a method of labelling a nucleic acid. Preferably, one would incorporate the compound described above into the nucleic acid chain in the same manner that one incorporates other nucleotides. In a most preferaole form of the invention, one would then determine the nucleic acid seσuence of the labelled nucleic acid molecule.
EXAMPLES
In General
The reaction schemes described below are general for several of the compounds made oy the metnod. "NTP" signifies a nucleoside, or nucleoside mono-, di-, or triphosphate, bound through an ammo group to the linker and dye. All the synthesized examples are triphosphates , as they are the most difficult, but also most useful, compounds to prepare. The following abbreviations are used: TABLE 1
Figure imgf000009_0001
Figure imgf000009_0002
Compounds prepared by the method described in Fig. 2 include :
IDC-rCTP IDC-dCTP IDC-ddCTP ITC-ddCTP ITC-ddATP IDC-dATP
IMC-c7-ddGTP
OMC-ddCTP
IMC-ddCTP A study using visible spectroscopy was done to determine whether the hydrophobic dyes aggregate in aqueous solution. The greater the shorter wavelength shoulder, the more aggregation is occurring. The concentration of indotπcarbocyanme-ddATP , one of the most hydrophobic dye-nucleoside conjugates, was varied over a range from 0.6 to 80 μM. The ratio of 744 nm : 682 nm was observed (the wavelength maximum to the shorter wavelength at the shoulder of ITC spectrum) . Variation was approximately ±5% over the entire range, compared to the ratio at a median absorption of A744 = 0.578 : A682 = 0.202, indicating very little, if any aggregation .
Experimental Procedures Example 1 (Fig. 5, r=2, X = C(CH3)2, R4=RS= H, R=(CH2)3OH, R3 =
5 ' -O-triphosphate, linker = DYE- (propy -0-PO--0- hexyl) -BASE; sugar = deoxyribosyl; base = adenme-N6.) 1-3"- (N6-hexyldeoxyadenosine, 5 ' -O- triphosphate) - propyl) phosphate) -1 ' - (3 '" -hydroxypropyl) )-3,3,3',3'- tetramethyl- indodicarbocyanine
(β-Cyanoethyl)
(1- (3 '" - (1'" -propyl) ) -l1 - (3"- (1"- (p-methoxytrityl) oxypropyl JJ-S/S^'^'- tetramethyl - indodicarbocyanine) ( 6 -N- trifluoroacetyla inohexyl ) phosphate The mono MMTr intermediate for the preparation of the corresponding amidite (1.13 g, 0.00145 mol), was treated with 6-N-trifluoroacetylaminohexyl -β-cyanoethyl - N,N-diisopropylamino-phosphoramidite (1.4 g, 0.0065 mol) and 0.4 g (0.0056 mol) of tetrazole (in 2 mL of dry acetonitrile) in 7 mL of dry dichloromethane . The reaction was stirred overnight, and then treated with 10 mL of 0.35 M iodine in a mixture of pyridine, water, and collidine. The solution was diluted with dichloromethane and extracted with aqueous bicarbonate and brine. The solution was dried and evaporated to leave the fully protected phosphotriester .
(1- (3 •"- (1'" -propyl) -1' - (3"-oxypropyl) ) -3,3,3 ■ ,3 ■- tetramethyl -indodicarbocyanine) (6-aminohexyl) phosphate
The triester was dissolved m 50 mL of ethanol , to which 150 mL of 3:1 cone, ammoma/ethanol were added. After two hours at 60°C, the TFA and cyanoethyl protecting groups had been removed. The solvents were evaporated and the residue was dissolved m 50 mL of 7% tπchloroacetic acid for one hour. The reaction mixture was neutralized by extraction with aqueous bicarbonate, dried, and evaporated. The residue was purified by C-18 NovaPak column chromatography with a 15 minute gradient of 40-100% acetonitrile in triethylammonium acetate, 0.1 M, pH 7.0. Rf = 7.8 minutes. The isolated material had the expected U /visible spectrum, λ = 648 nm.
(1- (3 '" - (1»" -propyl) -1' - (3" -oxypropyl) )-3,3,3',3'- tetramethyl - indodicarbocyanine ) ( 6 - aminohexyl N6-deoxyadenosine, 5 ' -O- triphosphate) phosphate
The aminohexyl -derivatized dye above was dissolved 500 μL of 0.1 M sodium borate, pH 9.2. To this was added 6-chloropurme-9- (1 ' -β-deoxyrιbosιde-5 ' -O- triphosphate) . The reaction was stirred overnight at 60°C, after which time HPLC (C-18, 0-70% acetonitrile/TEAA) analysis showed a high percentage of the product. The mam peak was isolated by prep C-18
HPLC, 10-50% acetonitrile/0.05 M ammonium phosphate, pH 7.2 over a 40 minute gradient. It was repurified twice by prep C-18 HPLC, 15-40% acetonitrile/0.05 ammonium phosphate, pH 7.2 over a 40 minute gradient to a purity of >99%. The product was desalted on a C-18 cartridge and stored m aqueous solution. The UV/visiole spectrum showed the expected peaks at 648 nm for the dye and 268 nm for an N6-deπvatιzed adenme.
The material was compared with Cy5-29,M-dATP , prepared by reaction of commercially obtained Cy5-29™-OSu (NHS ester) with N6-aminohexyl -dATP . The UV/visible spectra were identical, and the automated sequencing results obtained on ALFexpress (Pharmacia Biotech) were comparable. The sequencing results demonstrate that there is no difference m the reaction of the unsulfonated material described here, and the Cy5™-29-labeled material, which bears two sulfonate groups .
Example 2 (Fig. 5, r=2, X = C(CH3)2, R4=R= K, R=(CH2) OH, R =
5 ' -O-triphosphate, linker = DYE- (propyl -02C-ethyl -CO) - BASE; sugar = deoxyribosyl; base = cytosme-N4. 1-3"- (N4-6-amιdohexyldeoxycytidine-5 ' -O- triphosphate) - succinoyloxypropyl) -1' - (3 '" -hydroxypropyl) )-3,3,3',3'- tetramethyl -indodicarbocyanine (See Fig. 3)
(1- (3 "• - (1*" -Propyloxysuccinic acid) ) -l1 - (3"- (1"- (p-methoxytπtyl) oxypropyl) ) -3 , 3 , 3 ' , 3 ' -tetramethyl - indodicarbocyanine)
The mono MMTr intermediate for the preparation of the amidite (1 g, 0.00128 mol), was dissolved m 10 mL of pyridme and treated with 0.384 g (0.004 mol) succmic anhydride and 0.11 g 4-dιmethylammopyrιdme (0.0058 mol) . The reaction was stirred for 4 hours at ambient temperature. Progress was monitored by C-18 HPLC on a 3 μm column at 80% acetonitrile/TEAA, isocratic, detected at 648 nm. After the addition of 1 mL of water, the reaction was evaporated to dryness . The residue was dissolved m dichloromethane and was extracted with aqueous bicarbonate and brine. After drying, the organic layer was evaporated to dryness. (1- (3 '" - (1'" -Propyloxysuccinic acid) ) -1' -1.5*- (l7r- hydroxypropyl))-3,3,3',3'- tetramethyl - indodicarbocyanine )
The material from the previous reaction was dissolved in 30 mL of 80% acetic acid m water. After five hours at ambient temperature, the detritylation was complete, with no hydrolysis of the succmate ester. Progress was monitored by C-18 HPLC on a 3 μm column at 50% acetonitrile/TEAA, isocratic for 1 minute, then to 100% acetonitrile in 10 minutes, detected at 648 nm. The solution was evaporated and the residue dissolved m dichloromethane, extracted with aqueous bicarbonate three times, and brine. The solution was dried and evaporated to a blue powder.
(1- (3 "'- (1 '" -Propyloxysuccinic acid, N-hydroxysuccinimide ester) ) -1' - (3"- (1" -hydroxypropyl) ) -3,3, 3 ■ , 3 ' - tetramethyl - indodicarbocyanine)
The dry solid was dissolved in 10 mL of dry dichloromethane, followed by 0.5 mL pyrid e and 0.81 g (~3 eq) of 0- tr fluoroacetyl -N-hydroxysuccmimide . The reaction, monitored by C-18 HPLC on a 3 urn column at 50% acetonitrile/TEAA, isocratic for 1 minute, then to 100% acetonitrile m 10 minutes, detected at 648 nm, was over 5 minutes. Dichloromethane was added to 30 mL and the solution was extracted with water three times, dried, and evaporated.
1-3" - (N4-6-Amidohexyldeoxycytidine-5 ' -O- triphosphate) -succ inoyloxypropyl) -1 ' - (3 '" -hydroxypropyl) ) -3 , 3 , 3 ' , 3 ' -tetrame thyl-indodicarbocyanine
N4- (6 -Aminohexyl ) -dCTP was dissolved in 0.3 mL of 0.1 M sodium carbonate, pH 9.4. To this was added 200 μL of
DMF, followed by 100 μL of D F containing 10 mg of the aminolinker dye. The pH was readjusted to 9.4. The reaction was stirred for 1.5 hours at ambient temperature, at which time anion exchange HPLC (5-50% B in A over 30 minutes; A = 0.005 M sodium phosphate, pH 7.5 with 20% acetonitrile; B = A + 1 M NaCl ) analysis showed a high percentage of the product at ~6 minutes. The ma peak was repurified by C-18 HPLC, 5% for 2 minutes, then 5-60% acetonιtrιle/0.05 M ammonium phosphate, pH 7.2 over a 40 mmute gradient. It was repurified twice prep C-18 HPLC, 15-40% acetonιtrιle/0.05 M ammonium phosphate, pH 7.2 over a 40 mmute gradient to a purity of > 99 % . The product was desalted on a C-18 cartridge and stored aqueous solution. The UV/visible spectrum showed the expected peaks at 648 nm for tne dye and 276 nm for an N4 -derivatized cytosme.
The material was compared with Cy5-29™-dCTP, prepared by reaction of commercially obtained Cy5-29,M-OSu (NHS ester) with N4-ammonexyl-dCTP . The UV/visible spectra were identical, and the sequencing results obtained on ALFexpress (Pharmacia Biotech) were comparable. The sequencing results demonstrate that there is no difference in the reaction of the unsulfonated material described here, and the Cy5™-29-labeled material, which bears two sulfonate groups .
Example 3
(Fig. 5, r=2, X = C(CH3)2, R4=R5= K, R=(CH2),OH, R = 5 ' -O-tripnosphate, linker = DYE- (propyl -OjC-etnyi -CONH- hexyi)-BASE; sugar = ribosyl ; pase = cycosine-N1 ^
1-3" - ( (N4-6-Amidohexylcytιdine, 5 ' -O- triphosphate) - succinoyloxypropyl) -1 ' - (3 '" -hydroxypropyl) )-3,3,3',3'- tetramethyl - indodicarbocyanine
N4- (6 -Aminohexyl) -CTP (10 mg) , prepared from diammohexane and CTP by bisulfite catalysis, was dissolved m 200 μL sodium carbonate buffer, pH 9.5. 10 mg of the indodicarbocyanine -NHS ester (see Fig. 2, X = C(CH3)2, r = 2) was added in 50 μL of DMF. The pH was adjusted to 9.5 and the reaction was allowed to proceed for 2 hours. The product was isolated by NovaPak C18 HPLC (A = 0.05 M ammonium phosphate, pH 7.5, B = acetonitrile: 5% B for 2 minutes, 5-60% B for 40 minutes) . The appropriate peaks were pooled and desalted on a C-18 cartridge. The UV/visible spectrum showed the expected peaks at 646 nm for the dye and 276 nm for an N4-derιvatιzed cytosme.
Example 4
(Fig. 5, r=2, X = C(CH3)2, R4=R5= H, R=(CH2)3OH, R3 = 5 ' -O- triphosphate, linker = DYE- (propyl -02C-etnyl -CONH- hexyl) -BASE; sugar = dideoxyribosyl; base = cytosme-N4. ) 1-3"- ( (N4-6-Amidohexyl-2 ' , 3 ' -dideoxycytidine, 5 ■ -O- triphosphate) -succinoyloxypropyl) -1' - (3 '" -hydroxypropyl) ) -3 , 3 , 3 ' , 3 ' - tetramethyl - indodicarbocyanine N4- ( 6 -Ammonexyl ) -ddCTP, prepared from d ammohexane and 2 ' , 3 ' -dideoxy-CTP by bisulfite catalysis, (14 μmol) was dissolved m 1000 μL of 0.05 M sodium carbonate buffer, pH 9.5. To it was added 8 mg of the mdodicarbocyanme-NHS ester (see Fig. 2 , X = C(CH3)2, r = 2) m 150 μL of DMF and 150 μL water. The pH was adjusted to 9.3 and the reaction was allowed to proceed for 1 hour. The product was isolated by NovaPak C18 HPLC (A = 0.05 M ammonium phosphate, pH 7.2, B = acetonitrile: 0% B to 70% B for 40 minutes) . The appropriate peaks were pooled and desalted on a C-18 cartridge. The
UV/visible spectrum showed the expected peaks at 646 nm for the dye and 276 nm for an N -derιvatιzed cytosme. Yield: 59% of material absorbing at 646 nm, by HPLC analysis. The material was incorporated by a DNA polymerase m a standard sequencing assay, terminating chain extension.
Example 5
(Fig. 5, r=3, X = C(CH3)2, R4=R5= H, R=(CH2)3OH, R3 = 5 ' -O- triphosphate, linker = DYE- (propyl -02C-ethyl -CONH- hexyl) -BASE; sugar = dideoxyribosyl; base = cytosme-N4.) 1-3"- ( (N4-6-Amidohexyl-2 ' ,3 ' -dideoxycytidine, 5 ' -0- triphosphate) -succinoyloxypropyl) -1' -
(3 "■ -hydroxypropyl) ) -3,3,3 ' , 3 ' -tetramethyl - indotricarbocyanine
1,1" -Bis- (3"- (1-hydroxypropyl) ) -3,3,3 ' ,3 ' -tetramethyl- indotricarbocyanine)
1- ( (3 ' - (1 ' -Acetoxypropyl) ) -2,3 , 3-trιmethyl- (3H) - mdolmium iodide (2 g) and 0.71 g glutacondialdehyde dianil were dissolved m a mixture of 40 mL acetic anhydride, 10 mL acetic acid, and 1 g of potassium acetate. The solution was refluxed for 20 minutes, at which time the ratio of A740 to A280 indicated that the reaction was complete. The solvents were evaporated and the residue was dissolved m dichloromethane, extracted three times with aqueous bicarbonate and once with brine, and evaporated. The residue was dissolved in 100 mL of methanol and 100 mL 4 M HCl were added. The reaction was stirred at ambient temperature overnight to complete the hydrolysis of the acetate esters. The solvents were evaporated and the residue was dissolved m dichloromethane, extracted three times with aqueous bicarbonate and once with brine, and evaporated. HPLC confirmed the conversion to the title compound, compared to the diacetyl . UV/vis : λmax = 744 nm maximum
(1- (3 '" - (1'" -Propyloxysuccinic acid) ) -1 ' - (3" - ( 1" - hydroxypropyl) ) -3, 3, 3 ' , 3 ' - 1etra ethyundotπcarbocyanine)
1, 1"-Bιs- (3"- (1 -hydroxypropyl) )-3,3,3',3'- tetramethyl- indotricarbocyanine) (0.5 g) was co-evaporated twice with dry pyridme, dissolved m 10 mL of pyrid e and treated with 65 mg (1 eq . ) of succmic anhydride and 0.055 g 4 -dimethylammopyridme . The reaction was stirred for 4 hours at ambient temperature. Progress was monitored by C-18 HPLC on a 4 um column at 60% acetonitrile/TEAA, isocratic. After the addition of 1 mL of water, the reaction was evaporated to dryness. The residue was dissolved m 10 mL dichloromethane, extracted with water, and dried. After drying, the organic layer was evaporated to dryness. The residue was dissolved 10% acetonitrile m 1 M TEAA, pH 7 and purified on a prep HPLC on a NovaPak C18 cartridge with a gradient of 0-70% acetonιtrιle/0.1 M TEAA, pH 7. Yield: 30 mg.
(1_ (3 in . (i m -Propyloxysuccinic acid, N-hydroxysuccinimide ester) ) -1' - (3"- (1" -hydroxypropyl) )-3,3,3',3'- tetramethylindotricarbocyanine)
(1- (3 "' - (1 '" -Propyloxysuccinic acid) ) -1 ' - (3" - (1" -hydroxypropyl) ) -3, 3, 3 ' , 3 ' -tetramethyl - indotricarbocyanine) was dried by co-evaporation twice with dichloromethane, then dissolved m 1 mL of dry dichloromethane and 0.05 mL pyridme.
O-Trifluoroacetyl-N-hydroxysuccmimide (0.025 g) was added and the reaction was stirred. The reaction, monitored by C-18 HPLC on a 4 μm column with a gradient of 0-75% acetonitrile/TEAA, pH 7, detected at 648 nm, was over m 5 minutes. Dichloromethane was added to 30 mL and the solution was extracted with water tnree times, dried, and evaporated. UN/vis : λmax = 744 nm; yield: 34 mg at 80% purity.
1-3"- ( (N4-6-Amidohexyl-2 ' , 3 ' -dideoxycytidme, 5 ■ -O- triphosphate) -succinoyloxypropyl) -1' - (3 '" -hydroxypropyl) ) -3,3,3' , 3 ' -tetramethyl-indotricarbocyanine
N4- (6 -Aminohexyl) -ddCTP, prepared from diammohexane and 2 ', 3 ' -dideoxy-CTP by bisulfite catalysis, (6 mg, 10 μmol) was dissolved m 700 μL of 0.07 M sodium carbonate buffer, pH 9.5. To it was added 5 mg of the indotπcarbocyanme-NHS ester (Fig. 2, X = C(CH3)2, r = 3) 100 μL of DMF and 100 μL water. The pH was adjusted to 9.3 and the reaction was allowed to proceed for 45 minutes. The product was isolated by NovaPak CIS HPLC (A = 0.05 M ammonium phosphate, pH 7.2, B = acetonitrile: 0% B to 70% B for 40 minutes) . The appropriate peaks were pooled and desalted on a C-18 cartridge. The UV/visible spectrum showed the expected peaks at 744 nm for the dye and 274 nm for an N4-derivatιzed cytosme. Yield: 1.5 μmol . The material was incorporated by a DNA polymerase in a standard sequencing assay, terminating chain extension.
Example 6
(Fig. 5, r=3, X = C ( CHJ 2 , R4=R5= H, R=(CH2),OH, R-- = 5 ' -O- riphosphate, linker = DYE- (propyl -02C-ethyl -CONH- hexyl) -BASE ; sugar = deoxyribosyl; base = cytosme-N- . )
1-3"- ( (N4-6-Amidohex l-2 ' -deoxycytidine, 5 ' -O- triphosphate) -succinoyloxypropyl) -1' - (3 '" -hydroxypropyl) ) - 3 , 3 , 3 ' , 3 ' - tetramethylindotricarbocyanine N4- (6 -Aminohexyl) -dCTP, prepared from diammohexane and 2 ' -deoxy-CTP by bisulfite catalysis, (1 mg) was dissolved in 1000 μL of 0.1 M sodium carbonate buffer, pH 9.5. To it was added 1 mg of the mdotricarbocyanine-NHS ester (Fig. 3, X = C(CH3)2, r = 3) in 50 μL of DMF and 50 μL water. The pH was adjusted to 9.3 and the reaction was allowed to proceed for 45 minutes. The product was isolated by NovaPak C18 HPLC (A = 0.05 M ammonium phosphate, pH 7.2, B = acetonitrile: 0% B to 70% B for 40 minutes) . The appropriate peaks were pooled and desalted on a C-18 cartridge. The UV/visible spectrum showed the expected peaks at 744 nm for the dye and 274 nm for an N4-derivatιzed cytosme. Yield: 50% of material absorbing at 744 nm is product, by HPLC analysis.
Example 7 (Fig. 5, r=3, X = C(CH3)2, R4=R5= H, R=(CH2)3OH, R3 = 5' -0-triphosphate, linker = DYE- (propyl -02C-ethyl -CONH- hexyl) -BASE; sugar = dideoxyribosyl; base = adenine-N6.) 1-3"- ( (N6-6-Amidohexyl-2 ' , 3 ' -dideoxyadenosme, 5 ' -0- triphosphate) - succinoyloxypropyl) - 1 ' - ( 3 '" - hydroxypropyl) ) -3 , 3 , 3 ' , 3 ' -tetramethyl -indotricarbocyanine
N6- (6 -Aminohexyl ) -ddATP, prepared by reaction of diammohexane and 6-chloropurme-2 ' , 3 ' -dideoxyπboside- 5 ' -O-triphosphate (10 μmol) was dissolved in 500 μL of 0.08 M sodium carbonate buffer, pH 9.5. To it was added 3 mg of the indotricarbocyanine-NHS ester (Fig. 3 , X = C(CH3)2, r = 3) m 75 μL of DMF and 75 μL water. The pH was adjusted to 9.3 and the reaction was allowed to proceed for 2 hours. The product was isolated by NovaPak C18 HPLC (A = 0.05 M ammonium phosphate, pH 7.2, B = acetonitrile: 0% B to 70% B for 40 minutes) . The appropriate peaks were pooled, the acetonitrile evaporated, and the product stored in ammonium pnospnate solution. The UV/visible spectrum snowed tne expected peaks at 746 nm for the dye and 271 nm for an N6-suDΞtιtuted adenme. Yield: 48 nmol .
Example 8 (Fig. 5, r=l, X = C(CH3)2, R4=R5= H, R=(CH2)3OH, R3 = 5 ' -O-triphosphate, linker = DYE- (propyl -02C-ethyl-CONH- propynyl) -BASE; sugar = dideoxyribosyl; base = 7-deazaguanme-C . )
1-3"- ( (7- (3 -Amidopropynyl-2 ' , 3 ' -dideoxy-7-deazaguanosine, 5 ' -O-triphosphate) -succinoyloxypropyl) -1 ' - (3 "' - hydroxypropyl))-3,3,3',3'- tetramethyl- indomonocarbocyanine
(1- (3 "' - (1'" -Propyloxysuccinic acid) ) -1 ' - (3" - (1" -
(p-methoxytrityl) oxypropyl) ) -3 , 3 , 3 ' , 3 * -tetramethyl - indomonocarbocyanine)
The mono MMTr intermediate from the preparation of the IMC amidite (0.2 g) was co-evaporated twice with dry pyridme, dissolved m 2 mL of pyrid e, and treated with 0.077 g succmic anhydride and 0.022 g 4-dιmethylammopyrιdme . The reaction was stirred for 2 hours at ambient temperature. Progress was monitored by C-18 HPLC on a 3 μm column at 60% acetonitrile/TEAA, isocratic. After the addition of 0.2 mL of water, tne reaction was evaporated to dryness. The residue was dissolved m dichloromethane and was extracted itr. aqueous bicarbonate and brine. After drying, tne organic layer was evaporated to dryness. UV/vis : • λma> = 550 nm.
(1- (3 '" - (lm -Propyloxysuccinic acid) ) -1 ' - (3" - (1" - hydroxypropyl))-3,3,3',3'-tetramethyl- indomonocarbocyanine)
The material from the previous reaction was dissolved in 10 mL of 80% acetic acid in water. After three hours at ambient temperature, the detritylation was complete, with no hydrolysis of the succmate ester. Progress was monitored by C-18 HPLC. The solution was evaporated and the residue dissolved m dichloromethane, extracted with aqueous bicarbonate three times, and brine. The solution was dried and evaporated. Yield 130 mg . UV/vis: λraax = 550 nm.
(1- (3 '" - (l"1 -Propyloxysuccinic acid, N-hydroxysuccinimide ester) ) -1' - (3"- ( 1" -hydroxypropyl) )-3,3,3',3'- tetramethyl - indomonocarbocyanine)
The dry solid co-evaporated twice with dry pyridme, and was dissolved m 2 mL of dry dichloromethane, followed by 0.1 mL pyridme and 0.2 g (-3 eq) of
O-trifluoroacetyl-N-hydroxysuccmimide . The reaction, monitored by C-18 HPLC, was over in 5 minutes. Dichloromethane was added and the solution was extracted with water three times, dried, and evaporated. HPLC analysis (10-90% acetonitrile m 0.1 M TEAA, pH 7) showed the material to be about 85% pure. Yield 180 mg .
Figure imgf000020_0001
1-3"- ( (7- (3-Amidopropynyl-2 ' , 3 ' -dideoxy-7-deazaguanosme, 5 ' -O-triphosphate) -succinoyloxypropyl) -1 ' - (3 '" - hydroxypropyl) ) -3 , 3 , 3 ' , 3 ' -tetramethyl- indomonocarbocyanine
7- (3-Ammopropynyl) -7-deaza-2 ' , 3 ' -ddGTP (0.25 μmol), obtained from NEN/DuPont, was dissolved in 0.1 M aqueous carbonate buffer, pH 9.5. (1- (3 "'- (1 '" -Propyloxysuccinic acid, N-hydroxysuccinimide ester) ) -1 ' - (3" - (1" - hydroxypropyl) ) -3 , 3 , 3 ' , 3 ' - tetramethyl - indomonocarbocyanine) (1 mg) was added m 50 uL each of DMF and water. The pH was adjusted to 9.2. After 1 hour the reaction was quenched by the addition of NaH PC to pK 6.7. The product was purified on C-18 HPLC with acetonitrile and ammonium phosphate, pH 6. The appropriate peaks were pooled, the acetonitrile evaporated, and the product stored m ammonium phosphate solution. Yield: 29 nmol . UV/vis: λmax = 550 nm.
Example 9
(Fig. 5, r=l, X = C(CH3)2, R4=R5= H, R=(CH2)3OH, R3 = 5 ' -O-triphosphate, linker = DYE- (propyl -02C-ethyl -CONH- hexyl)-BASE; sugar = deoxyribosyl; base = cytosme-N4.)
1-3"- ( (N4- (6-Amidohexyl-2 ' -deoxycytidine, 5 ' -O- triphosphate) - succinoyloxypropyl) - 1 ' - ( 3 '" -hydroxypropyl) ) -3 , 3 , 3 ' , 3 ' - tetramethyl- indomonocarbocyanine
N4- (6-Ammonexyl ) -dCTP '1 mg) , prepared from diammohexane and 2 ' -deoxy-CTP by bisulfite catalysis, was dissolved in 0.1 M aqueous carbonate buffer, pH 9.5. (1- (3 - (1 '" -propyloxysuccinic acid, N-hydroxysuccmimide ester) )-l'-(3"-(l"- hydroxypropyl) )-3,3,3',3'- tetramethyl- indomonocarbocyanine) (1 mg) was added in 50 μL each of DMF and water. The pH was adjusted to 9.2.
After 1 hour the presence of the product was confirmed by HPLC by comparison to similar compounds. UV/vis: λmax = 550 n . Exampl e 10
(Fig. 5, r=l, X = 0, R4=RS= H, R=(CH2),OH, R; = 5 ' -O-triphosphate, linker = DYE- (propyl -02C-ethyl -CONH- hexyl)-BASE; sugar = dideoxyribosyl; base = cytosme-N4.) 1-3"- ( (N4-6-Amidohexyl-2' ,3 ' -dideoxycytidine, 5'-0- triphosphate) -succinoyloxypropyl) -1 ' - (3 '" -hydroxypropyl) ) -benzoxazolmonocarbocyanine
1- ( (3 ' - (1 ' -Acetoxypropyl) ) -benzoxazolium iodide
2-Methylbenzoxaole (7.0 g 0.053 mol) and 13.2 g (0.03 mol) of 3-iodopropyl acetate were heated together at 100-110°C for 16 hours. The mixture was crystallized to a granular powder by trituration m ethyl acetate. It was filtered and dried by washing with etner. Yield: 15.1 g.
1,1" -Bis- (3"- (1-acetoxypropyl) ) -benzoxazolmonocarbocyanine
1- ( (3 ' - (1 ' -Acetoxypropyl) ) -benzoxazolium iodide (5 g, 0.014 mol) and 4.5 g of triethylorthoformate were dissolved in 100 mL dry pyridine. The solution was refluxed for three hours. The solvents were evaporated and the residue crystallized from ethyl acetate. Yield: 4.9 g. UV/vis: λmax = 484 nm.
(1- (3"- (1"-acetoxypropyl) -1"- (3 '" - (1'" -hydroxypropyl) ) - benzoxazolmonocarbocyanine
1- ( (3 ' - (1 ' -Acetoxypropyl) ) -benzoxazolium iodide (1 g) was stirred m a mixture of 20 mL of 4 N HCl and 20 mL methanol for 1 hour, then was rotovaped to dryness. The residue was dissolved m dichloromethane and extracted with water. The water was back-extracted three times with dichloromethane. The organic layers were combined, dried, and evaporated. The material was purified by C18 prep HPLC on a 25 x 200 mm Novapak cartridge using a 10- 65% acetonitrile/TEAA gradient. The fractions enriched in the mono-acetyl derivative were pooled and evaporated to dryness. (1- (3 '"- (1"' -Propyloxysuccinic acid) ) -1' - (3 »•• - (1"' - acetoxypropyl) ) -benzoxazolmonocarbocyanine
The mono-acetyl derivative (175 mg) was dried by co- evaporation three times with dry acetonitrile and dissolved m 1 mL of dry pyridme. Succmic anhydride and DMAP were added and the reaction proceeded for 2 hours. The reaction was quenched with 1 mL water and the product purified by C18 prep HPLC on a 25 x 100 mm Novapak cartridge using a 0-80% acetonitrile/TEAA gradient. Yield: 22 mg.
(1- (3 '"- (1 '" -Propyloxysuccinic acid N-hydroxysuccinimide ester) ) -1' - (3 "■ - (1MI -acetoxypropyl) ) - benzoxazolmonocarbocyanine
The succ ate derivative (22 mg) was dried by co- evaporation two times with dry pyridme and dissolved 1 mL of dry dichloromethane with 50 μL of dry pyrid e. O-Trifluoroacetyl -N-hydroxysuccmimide (100 mg) was added. After 5 minutes the reaction was diluted with 5 mL dichloromethane and extracted twice with water. The dichloromethane was dried and evaporated to yield 20 mg of activated ester.
1-3"- ( (N4-6-Amidohexyl-2 ' , 3 ' -dideoxycytidine, 5 ' -O- triphosphate) -succinoyloxypropyl) -1' - (3"' - hydroxypropyl) ) -benzoxazolmonocarbocyanine The activated ester (2 mg) was dissolved m 200 μL 50% DMF/water and 4 μmol of 6 -aminohexyl-ddCTP in 500 μL 0.1 M carbonate buffer, pH 9 was added. After 45 minutes the reaction was terminated. The product was purified by C18 HPLC (3.9 x 150 mm Novapak, 0-75% acetonitπle/ammonium phosphate, pH 6. Yield: 584 nmol .
Figure imgf000023_0001
The material was incorporated by a DNA polymerase in a standard sequencing assay, terminating chain extension. REFERENCES
Hamer, "Cyanme Dyes and Related Compounds," Interscience Publishers, pp. 86-350, 1964.
Sturmer et. aJ . , "Sensitizing and Desensitizing Dyes," Special Topics m Heterocyclic Chemistry, Ch. 8, pp. 194-197, 1977.
(a) Southwick, Ernst, Tauriello, Parker, Mujumdar, Mujumdar, Clever, and Waggoner, "Cyanme Dye Labeling Reagents - Carboxymethylmdocyanme Succmimidyl Esters," Cytometry 11:418-430, 1990.
(b) Yu, Ernst, Wagner, and Waggoner, "Sensitive Detection of RNAs in Single Cells by Flow Cytometry," Nuc . Acids Res . 20:83-88, 1992.
(c) Galbraith, Wagner, Chao, Abaza, Ernst, Nederlof, Hartsock, Taylor, and Waggoner, Cytometry, 12:579-596, 1991.
(d) Ernst, Gupta, Mujumdar, and Waggoner, Cytometry, 10:3-10, 1989.
(a) Mujumdar, Ernst, Mujumdar, Lewis, and Waggoner, "Cyanme Dye Labeling Reagents :
Sulfomdocyanme Succmimidyl Esters," Bioconj uga te Chem . 4:105-111, 1993.
(b) Mujumdar, Mujumdar, Grant, and Waggoner, "Cyanme Dye Labeling Reagents:
Sulfobenz (e) mdocyanme Succmimidyl Esters," Bioconj uga te Chem . 7:356-362, 1996.
(c) US 4 981, 977 1/91, Southwick and Waggoner.
(d) us 5 268 486 12/93, Waggoner, et. al .
(e) us 5 486 616 1/96, Waggoner, et. al .
(f) us 5 569 587 10/96, Waggoner.
(g) us 5 569 766 10/96, Waggoner, et. al .
(a) us 5 047 519 9/91, Hobbs and Cucozza.
(b) us 5 151 507 9/92, Hobbs and Trainor.
(c) us 5 242 796 9/93, Prober, et. al .
(d) us 5 332 666 7/94, Prober, e al .
(e) us 5 558 991 9/96, Trainor.
(f) us 4 828 979 5/89, Klevan, et. al . (g) PCT WO 95/04747, Mϋhlegger, et aJ . (h) PCT/EP92/01756, Ansorge , et al.
(a) US 4,711,955; 12/87, Ward, et al .
(b) US 5,328,824; 7/94; Ward, et al. (c) US 5,449,767; 9/95, Ward, et al.
(d) US 5,476,928; 12/95, Ward, e_t aJ .
(e) US 5,241,060; 8/93, Englehart , et. al -
7. (a) Yu, Chao, Patek, Mujumdar, Mujumdar, and
Waggoner, "Cyanme Dye dUTP Analogs For Enzymatic Labeling Of DNA Probes," Nuc. Acids Res . 22:3226-3232, 1994.
(b) Amersham Life Science Catalogue, 1996.
8. Johnson, Zhang, and Bergstrom, "The synthesis and stability of oligodeoxyribonucleotides containing deoxyadenosme mimic 1- (2 ' -deoxy-β-D-ribofuranosyl) rmidazole-4-carboxamide, " Nuc . Acids Res . 25:559- 567, 1997.

Claims

CLAIMS We claim:
1. A chemical compound of the following formula:
R3-0- Sug
Figure imgf000026_0001
wnerem:
R1 is selected from the group consisting of alkyl, aralkyl , and substituted alkyl groups; R3 is selected from the group consisting of H,
P03 "2; P206 "3; P309"4, α-thio phosphates; and cBH3 phosphates ;
R4 is selected from the group consisting of H, lower alkyl, acyl, (CH2) PC00 (CH2) qCH, wherein p is an integer from O to 4 and q is an integer from O to 4 , and 5,6; 6,7; or 7,8 -butadienyl ;
R5 is selected from the group consisting of H, lower alkyl, acyl, (CH2) pCOO (CH2) qCH3 wherein p is an integer from O to 4 and q is an integer from O to 4 and 5,6; 6,7; or 7 , 8 -butadienyl ; r is 1, 2, or 3;
X or Y are selected from the group consisting of 0, S, C(R6)2, N(R6) , wherein R6 is CH3 or a lower alkyl; and R3-0-Sugar-Base is a nucleoside or nucleotide .
2. The compound of claim 1 wherein R is selected from the group consisting of substituted alkyl chains, wherein the substitution is OR2, COOR2 , NR2R2 , or SR2 , wherein R2 is H, a removable protecting group or a lower alkyl group .
3. The compound of claim 2 wherein R2 is selected from the group consisting of H, a removable protecting group, or a lower alkyl group.
4. The compound of claim 1 wherein R is (CH2),OH.
5. The compound of claim 1 wherein R is selected from the group consisting of (CH2)=.COOH, (CH2) ,NH2 and C2H-..
6. The compound of claim 1 wherein R3 is selected from the group consisting of P03 2 , P206 3 and P309 4.
7. The compound of claim 1 wherein the linker is selected from the group consisting of propyl -0-P02-0- hexyl, propyl -02C-ethyl -CO, propyl -02C-ethyl -CONH-hexyl , and propyl -02C-ethyl-CONH-propynyl .
8. The compound of claim 1 wherein the linker is between 3 and 25 atoms m length.
9. The compound of claim 1 wherein X and Y are C(CH3)2.
10. The compound of claim 1 wherein the nucleotide or nucleoside formed by R3-0-Sugar-Base is selected from the group consisting of IDC-rCTP, IDC-dCTP, IDC-ddCTP, ITC-ddCTP, ITC-ddATP, IDC-dATP, IMC-c7-ddGTP, and OMC-ddCTP.
11. The compound of claim 1 wherein R3 is selected from the group consisting of PS02 2; P2S05"3; and P3S0 -4
8
12. The compound of claim 1 wherein R3 is selected from the group consisting of P(BH3)02 _z, P2(BH,)05 3, and P3(BH3)08-4.
13. A non-sulfonated carbocyanine dye linked to a nucleotide or nucleoside.
14. The dye of claim 13 wherein the dye is a indocarbocyanine dye .
15. A method of labeling a nucleic acid molecule comprising the step of incorporating the compound of claim 1 into a nucleic acid chain.
16. The method of claim 15 further comprising the step determining the nucleic acid sequence of the molecule .
17. A method of labeling a nucleic acid molecule comprising the step of incorporating the compound of claim 13 into a nucleic acid chain.
18. The method of claim 17 further comprising the step of determining the nucleic acid sequence of the molecule .
PCT/US1998/012593 1997-06-20 1998-06-16 Non-sulfonated cyanine dyes for labeling nucleosides and nucleotides Ceased WO1998058942A1 (en)

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Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001019841A1 (en) * 1999-09-17 2001-03-22 Amersham Pharmacia Biotech, Inc. Charge-modified nucleic acid terminators
EP1060264A4 (en) * 1998-02-04 2001-07-04 Amersham Pharm Biotech Inc Dideoxy dye terminators
JP2001288197A (en) * 2000-04-10 2001-10-16 Fuji Photo Film Co Ltd Fluorescent nucleotide
WO2002026890A1 (en) * 2000-09-19 2002-04-04 Institut für Chemo- und Biosensorik Münster E.V. 3-spiro-cyanin fluorochromes and their use in bioassays
JP2003034696A (en) * 2001-07-19 2003-02-07 Fuji Photo Film Co Ltd Fluorescent nucleotide and labeling method using the same
US6811979B2 (en) * 2000-10-11 2004-11-02 Applera Corporation Fluorescent nucleobase conjugates having anionic linkers
EP1112254A4 (en) * 1999-04-19 2005-03-16 Nen Life Science Prod Inc Cyanine dyes and synthesis methods thereof
FR2913424A1 (en) * 2007-03-09 2008-09-12 Cis Bio Internat Sa CYANINE DERIVATIVES, FLUORESCENT CONJUGATES CONTAINING SAME AND USE THEREOF
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JP2012184246A (en) * 1999-08-27 2012-09-27 Cibus Internatl Lp Single-stranded oligonucleotide mutational vector

Families Citing this family (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1088559A3 (en) * 1999-09-29 2002-10-02 INSTITUT FÜR DIAGNOSTIKFORSCHUNG GmbH AN DER FREIEN UNIVERSITÄT BERLIN Galenic formulations
US6642375B2 (en) 1999-12-07 2003-11-04 Fuji Photo Film Co., Ltd. Fluorescent substances
JP2003531111A (en) * 1999-12-17 2003-10-21 アライアッド・ファーマシューティカルズ・インコーポレーテッド Proton pump inhibitor
AU2583901A (en) * 1999-12-17 2001-06-25 Ariad Pharmaceuticals, Inc. Proton pump inhibitors
CA2423806C (en) 2000-09-29 2009-12-22 Molecular Probes, Inc. Modified carbocyanine dyes and their conjugates
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AU2002306620A1 (en) * 2001-02-28 2002-09-12 Stratagene Fluorescent dye
US6972339B2 (en) 2001-09-07 2005-12-06 Epoch Biosciences, Inc. Compounds and methods for fluorescent labeling
US8569516B2 (en) * 2001-09-07 2013-10-29 Elitech Holding B.V. Compounds and methods for fluorescent labeling
IL146142A0 (en) * 2001-10-24 2002-07-25 Univ Bar Ilan 2-substituted-5'-o-(1-boranotriphosphate adenosine derivatives and pharmaceutical compositions comprising them for treatment of type 2 diabetes
US6838289B2 (en) 2001-11-14 2005-01-04 Beckman Coulter, Inc. Analyte detection system
WO2004003510A2 (en) * 2002-07-01 2004-01-08 Guava Technologies, Inc. Fluorescent dyes, energy transfer couples and methods
US20040265870A1 (en) * 2003-04-09 2004-12-30 Invitrogen Corporation Methods of synthesizing and labeling nucleic acid molecules
US20050065171A1 (en) * 2003-06-25 2005-03-24 Shakespeare William C. Substituted purine derivatives
US7570443B2 (en) 2003-09-19 2009-08-04 Applied Biosystems, Llc Optical camera alignment
US20060029948A1 (en) * 2003-09-19 2006-02-09 Gary Lim Sealing cover and dye compatibility selection
US7417726B2 (en) * 2003-09-19 2008-08-26 Applied Biosystems Inc. Normalization of data using controls
WO2005040357A2 (en) * 2003-10-24 2005-05-06 Epoch Biosciences, Inc. Compounds and methods for fluorescent labeling
US7705150B2 (en) 2004-02-04 2010-04-27 Biosearch Technologies, Inc. Cyanine dyes
WO2005077125A2 (en) * 2004-02-11 2005-08-25 Applera Corporation Methods and compositions for detecting nucleic acids
US20050255485A1 (en) * 2004-05-14 2005-11-17 Livak Kenneth J Detection of gene duplications
JP5214967B2 (en) 2004-08-13 2013-06-19 エポック バイオサイエンシズ インコーポレーティッド Phosphonic acid fluorescent dyes and complexes
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US7781187B2 (en) * 2005-12-30 2010-08-24 Corning Incorporated Fluorescent dyes
WO2009012109A2 (en) 2007-07-13 2009-01-22 Emory University Cyanine-containing compounds for cancer imaging and treatment
WO2009152440A1 (en) * 2008-06-13 2009-12-17 Cedars-Sinai Medical Center Small molecule ligand-drug conjugates for targeted cancer therapy
JP4621926B2 (en) * 2008-07-24 2011-02-02 国立大学法人九州大学 Enzyme substrate-modified nucleoside triphosphate, nucleic acid probe, multilabeled nucleic acid probe, method for producing multilabeled nucleic acid probe, and method for detecting target nucleic acid
JP5146785B2 (en) * 2008-07-24 2013-02-20 国立大学法人九州大学 Enzyme substrate modified nucleoside triphosphate derivatives
JP5704481B2 (en) * 2010-01-22 2015-04-22 国立大学法人九州大学 Nucleic acid detection kit
US9777320B2 (en) 2010-08-25 2017-10-03 Pacific Biosciences Of California, Inc. Molecular adaptors for dye conjugates
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ES2537189T3 (en) 2011-05-24 2015-06-03 Elitech Holding B.V. Detection of methicillin-resistant Staphylococci
US8889884B1 (en) 2011-07-14 2014-11-18 Pierce Biotechnology, Inc. Phosphine derivatives of fluorescent compounds
US9249307B2 (en) 2011-08-16 2016-02-02 Pierce Biotechnology, Inc. Benzocyanine compounds
US9751868B2 (en) 2012-02-28 2017-09-05 Pierce Biotechnology, Inc. Benzocyanine compounds
WO2013130761A1 (en) 2012-03-02 2013-09-06 Pierce Biotechnology, Inc. Indole derivatives as labeling dye for biomolecule
EP2850086B1 (en) 2012-05-18 2023-07-05 Pacific Biosciences Of California, Inc. Heteroarylcyanine dyes
US9315864B2 (en) 2012-05-18 2016-04-19 Pacific Biosciences Of California, Inc. Heteroarylcyanine dyes with sulfonic acid substituents
JP5509440B2 (en) 2012-06-22 2014-06-04 国立大学法人九州大学 Method for producing protein-nucleic acid complex and method for detecting target
US9676787B2 (en) 2012-08-28 2017-06-13 Pierce Biotechnology, Inc. Benzopyrylium compounds
EP2894158B1 (en) 2012-09-04 2018-06-20 Kabushiki Kaisha DNAFORM Compound, nucleic acid, labeling substance, and detection method
WO2014164479A1 (en) 2013-03-11 2014-10-09 Elitech Holding B.V. Methods for true isothermal strand displacement amplification
US9328384B2 (en) 2013-05-13 2016-05-03 Elitechgroup B.V. Droplet digital PCR with short minor groove probes
EP3230467A1 (en) 2014-12-12 2017-10-18 ELITechGroup B.V. Methods and compositions for detecting antibiotic resistant bacteria
US9988670B2 (en) 2014-12-12 2018-06-05 Elitechgroup B.V. Methods and compositions for detecting antibiotic resistant bacteria
WO2021080629A1 (en) 2019-10-23 2021-04-29 Elitechgroup, Inc. Methods for true isothermal strand displacement amplification
CN115181049B (en) * 2022-06-01 2023-12-19 郑州玛特瑞斯生物科技有限公司 Fluorescent compound, preparation method thereof, fluorescent modified nucleotide and sequencing reagent

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996022298A1 (en) * 1995-01-18 1996-07-25 Pharmacia Biotech Inc. Method and composition for stabilization of labelled nucleoside triphosphates
US5556959A (en) * 1993-01-22 1996-09-17 Pharmacia P-L Biochemicals Inc. Indocarbocyanine-linked phosphoramidites

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5569587A (en) * 1986-04-18 1996-10-29 Carnegie Mellon University Method for labeling and detecting materials employing luminescent arysulfonate cyanine dyes
US5268486A (en) * 1986-04-18 1993-12-07 Carnegie-Mellon Unversity Method for labeling and detecting materials employing arylsulfonate cyanine dyes
US5627027A (en) * 1986-04-18 1997-05-06 Carnegie Mellon University Cyanine dyes as labeling reagents for detection of biological and other materials by luminescence methods
US4981977A (en) * 1989-06-09 1991-01-01 Carnegie-Mellon University Intermediate for and fluorescent cyanine dyes containing carboxylic acid groups
GB9009980D0 (en) * 1990-05-03 1990-06-27 Amersham Int Plc Phosphoramidite derivatives,their preparation and the use thereof in the incorporation of reporter groups on synthetic oligonucleotides
DE4326466A1 (en) * 1993-08-06 1995-02-09 Boehringer Mannheim Gmbh Infrared dye-labeled nucleotides and their use in nucleic acid detection

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5556959A (en) * 1993-01-22 1996-09-17 Pharmacia P-L Biochemicals Inc. Indocarbocyanine-linked phosphoramidites
WO1996022298A1 (en) * 1995-01-18 1996-07-25 Pharmacia Biotech Inc. Method and composition for stabilization of labelled nucleoside triphosphates

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
J.B. RANDOLPH AND A.S. WAGGONER: "Stability, specifity and fluorescence brightness of multiply-labeled fluorescent DNA probes", NUCLEIC ACIDS RES., vol. 25, no. 14, 15 July 1997 (1997-07-15), pages 2923 - 29, XP002074426 *
Z. ZHU ET AL.: "Directly labeled DNA probes using fluorescent nucleotides with different length linkers", NUCLEIC ACIDS RES., vol. 22, 1994, pages 3418 - 22, XP002074425 *

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US6949635B1 (en) 1998-02-04 2005-09-27 Amersham Biosciences Corp Dideoxy dye terminators
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US7855293B2 (en) 2000-09-19 2010-12-21 Lutz Haalck 3-spiro-cyanin fluorochromes and their use in bioassays
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US7687236B2 (en) 2000-10-11 2010-03-30 Applied Biosystems, Llc Fluorescent nucleobase conjugates having anionic linkers
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US8383792B2 (en) 2007-03-09 2013-02-26 Riken Compound having structure derived from mononucleoside or mononucleotide, nucleic acid, labeling substance, and method and kit for detection of nucleic acid
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