WO1999013330A3 - Aufreinigung von substanzen aus einer biologischen probe - Google Patents

Aufreinigung von substanzen aus einer biologischen probe Download PDF

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Publication number
WO1999013330A3
WO1999013330A3 PCT/EP1998/005521 EP9805521W WO9913330A3 WO 1999013330 A3 WO1999013330 A3 WO 1999013330A3 EP 9805521 W EP9805521 W EP 9805521W WO 9913330 A3 WO9913330 A3 WO 9913330A3
Authority
WO
WIPO (PCT)
Prior art keywords
biological sample
purification
substances
target substance
binding pair
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP1998/005521
Other languages
English (en)
French (fr)
Other versions
WO1999013330A2 (de
Inventor
Wolfgang Hoesel
Helmut Lenz
Jochen Peter
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Roche Diagnostics GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics GmbH filed Critical Roche Diagnostics GmbH
Priority to DE59813433T priority Critical patent/DE59813433D1/de
Priority to JP2000511063A priority patent/JP4150500B2/ja
Priority to US09/508,138 priority patent/US6635420B1/en
Priority to EP98952583A priority patent/EP1015885B1/de
Publication of WO1999013330A2 publication Critical patent/WO1999013330A2/de
Publication of WO1999013330A3 publication Critical patent/WO1999013330A3/de
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/422—Displacement mode
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/803—Physical recovery methods, e.g. chromatography, grinding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification
    • Y10S435/815—Enzyme separation or purification by sorption
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/971—Capture of complex after antigen-antibody reaction
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/823—Immunogenic carrier or carrier per se
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/824—Immunological separation techniques

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Die Erfindung betrifft ein Verfahren zur Aufreinigung einer Zielsubstanz aus einer biologischen Probe durch Immobilisierung der Zielsubstanz an einer Festphase über ein hochaffines Bindepaar und anschließende Elution durch Zugabe eines Partners des Bindepaares in freier Form. Weiterhin werden Reagenzienkits zur Duchführung des Verfahrens offenbart.
PCT/EP1998/005521 1997-09-08 1998-08-31 Aufreinigung von substanzen aus einer biologischen probe Ceased WO1999013330A2 (de)

Priority Applications (4)

Application Number Priority Date Filing Date Title
DE59813433T DE59813433D1 (de) 1997-09-08 1998-08-31 Aufreinigung von substanzen aus einer biologischen probe
JP2000511063A JP4150500B2 (ja) 1997-09-08 1998-08-31 生物学的試料からの物質の精製
US09/508,138 US6635420B1 (en) 1997-09-08 1998-08-31 Purification of substances from a biological sample
EP98952583A EP1015885B1 (de) 1997-09-08 1998-08-31 Aufreinigung von substanzen aus einer biologischen probe

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19739218A DE19739218A1 (de) 1997-09-08 1997-09-08 Aufreinigung von Substanzen aus einer biologischen Probe
DE19739218.0 1997-09-08

Publications (2)

Publication Number Publication Date
WO1999013330A2 WO1999013330A2 (de) 1999-03-18
WO1999013330A3 true WO1999013330A3 (de) 1999-06-17

Family

ID=7841541

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1998/005521 Ceased WO1999013330A2 (de) 1997-09-08 1998-08-31 Aufreinigung von substanzen aus einer biologischen probe

Country Status (7)

Country Link
US (1) US6635420B1 (de)
EP (1) EP1015885B1 (de)
JP (1) JP4150500B2 (de)
AT (1) ATE319735T1 (de)
DE (2) DE19739218A1 (de)
ES (1) ES2259213T3 (de)
WO (1) WO1999013330A2 (de)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
DE19739218A1 (de) 1997-09-08 1999-03-11 Boehringer Mannheim Gmbh Aufreinigung von Substanzen aus einer biologischen Probe
US7569342B2 (en) 1997-12-10 2009-08-04 Sierra Molecular Corp. Removal of molecular assay interferences
JP2005538929A (ja) 2002-01-16 2005-12-22 ダイナル バイオテック エイエスエイ 単一サンプルからの核酸及びタンパク質の単離方法
US7597936B2 (en) * 2002-11-26 2009-10-06 University Of Utah Research Foundation Method of producing a pigmented composite microporous material
US7682688B2 (en) * 2002-11-26 2010-03-23 University Of Utah Research Foundation Microporous materials, methods, and articles for localizing and quantifying analytes
GB0229287D0 (en) * 2002-12-16 2003-01-22 Dna Res Innovations Ltd Polyfunctional reagents
EP2143482A1 (de) * 2003-02-19 2010-01-13 Natrix Separations Inc. Geträgerte poröse Gele umfassende Verbundmaterialien
AU2005231532B2 (en) * 2004-04-08 2010-01-07 Merck Millipore Ltd. Membrane stacks
US8133840B2 (en) * 2004-06-07 2012-03-13 Natrix Separations Inc. Stable composite material comprising supported porous gels
US7492312B2 (en) * 2006-11-14 2009-02-17 Fam Adly T Multiplicative mismatched filters for optimum range sidelobe suppression in barker code reception
CA2736814C (en) * 2008-09-02 2017-02-28 Natrix Separations Inc. Chromatography membranes, devices containing them, and methods of use thereof
KR20170104656A (ko) * 2009-11-13 2017-09-15 나트릭스 세퍼레이션즈, 인코포레이티드 소수성 상호반응 크로마토그래피용 막 및 그의 사용방법
EP2709749B1 (de) 2011-05-17 2018-08-15 Natrix Separations Inc. Verwendungsverfahren für gewickelte chromatographie-membranen

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0378197A2 (de) * 1989-01-11 1990-07-18 Roche Diagnostics GmbH Vitamin-B12-Bestimmung
WO1992002818A1 (en) * 1990-08-10 1992-02-20 Purdue Research Foundation Matrix sequential addition immunoassay
EP0506032A1 (de) * 1991-03-28 1992-09-30 American Cyanamid Company Rezeptorreinigungsverfahren

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3225027A1 (de) * 1982-07-05 1984-01-05 Boehringer Mannheim Gmbh, 6800 Mannheim Immunchemisches messverfahren
DE3430905A1 (de) * 1984-08-22 1986-02-27 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren zur bestimmung einer immunologisch bindefaehigen substanz
DE3900534A1 (de) * 1989-01-10 1990-07-12 Boehringer Mannheim Gmbh Diagnostischer nachweis unter verwendung von chimaeren antikoerpern
EP0440044A1 (de) * 1990-01-31 1991-08-07 Abbott Laboratories Verfahren zur Vermeidung humaner Anti-Maus-Antikörper-Störungen in den in vitro diagnostischen Bestimmungsverfahren
DE19739218A1 (de) 1997-09-08 1999-03-11 Boehringer Mannheim Gmbh Aufreinigung von Substanzen aus einer biologischen Probe

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0378197A2 (de) * 1989-01-11 1990-07-18 Roche Diagnostics GmbH Vitamin-B12-Bestimmung
WO1992002818A1 (en) * 1990-08-10 1992-02-20 Purdue Research Foundation Matrix sequential addition immunoassay
EP0506032A1 (de) * 1991-03-28 1992-09-30 American Cyanamid Company Rezeptorreinigungsverfahren

Also Published As

Publication number Publication date
JP4150500B2 (ja) 2008-09-17
DE59813433D1 (de) 2006-05-04
US6635420B1 (en) 2003-10-21
DE19739218A1 (de) 1999-03-11
WO1999013330A2 (de) 1999-03-18
EP1015885B1 (de) 2006-03-08
JP2001515788A (ja) 2001-09-25
ATE319735T1 (de) 2006-03-15
EP1015885A2 (de) 2000-07-05
ES2259213T3 (es) 2006-09-16

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