WO1999034003A3 - Proteases from gram positive organisms - Google Patents

Proteases from gram positive organisms Download PDF

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Publication number
WO1999034003A3
WO1999034003A3 PCT/US1998/027020 US9827020W WO9934003A3 WO 1999034003 A3 WO1999034003 A3 WO 1999034003A3 US 9827020 W US9827020 W US 9827020W WO 9934003 A3 WO9934003 A3 WO 9934003A3
Authority
WO
WIPO (PCT)
Prior art keywords
present
metalloprotease
gram positive
provides
proteases
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1998/027020
Other languages
French (fr)
Other versions
WO1999034003A2 (en
Inventor
David A Estell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Genencor International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor International Inc filed Critical Genencor International Inc
Priority to US09/554,998 priority Critical patent/US6599731B1/en
Priority to AU19301/99A priority patent/AU1930199A/en
Priority to EP98964108A priority patent/EP1042490A2/en
Publication of WO1999034003A2 publication Critical patent/WO1999034003A2/en
Publication of WO1999034003A3 publication Critical patent/WO1999034003A3/en
Anticipated expiration legal-status Critical
Priority to US11/013,992 priority patent/US20050101504A1/en
Priority to US11/014,361 priority patent/US7098021B2/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present invention relates to the identification of a novel metalloprotease in gram positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the metalloprotease. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding the metalloprotease. The present invention provides host cells which further comprises a nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions, animal feeds and compositions used to treat a textile that include the metalloprotease of the present invention.
PCT/US1998/027020 1997-12-30 1998-12-17 Proteases from gram positive organisms Ceased WO1999034003A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
US09/554,998 US6599731B1 (en) 1997-12-30 1998-12-17 Proteases from gram positive organisms
AU19301/99A AU1930199A (en) 1997-12-30 1998-12-17 Proteases from gram positive organisms
EP98964108A EP1042490A2 (en) 1997-12-30 1998-12-17 Proteases from gram positive organisms
US11/013,992 US20050101504A1 (en) 1997-12-30 2004-12-16 Proteases from gram positive organisms
US11/014,361 US7098021B2 (en) 1997-12-30 2004-12-16 Proteases from gram positive organisms

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9727464.1A GB9727464D0 (en) 1997-12-30 1997-12-30 Proteases from gram positive organisms
GB9727464.1 1997-12-30

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US09554998 A-371-Of-International 1998-12-17
US10/460,718 Division US6872807B2 (en) 1997-12-30 2003-06-12 Proteases from gram positive organisms

Publications (2)

Publication Number Publication Date
WO1999034003A2 WO1999034003A2 (en) 1999-07-08
WO1999034003A3 true WO1999034003A3 (en) 1999-11-18

Family

ID=10824308

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/027020 Ceased WO1999034003A2 (en) 1997-12-30 1998-12-17 Proteases from gram positive organisms

Country Status (4)

Country Link
EP (1) EP1042490A2 (en)
AU (1) AU1930199A (en)
GB (1) GB9727464D0 (en)
WO (1) WO1999034003A2 (en)

Families Citing this family (60)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9727470D0 (en) * 1997-12-30 1998-02-25 Genencor Int Bv Proteases from gram positive organisms
BR0214653B1 (en) 2001-12-07 2015-01-06 Novozymes As CONSTRUCTION OF NUCLEIC ACID, RECOMBINANT EXPRESSION VECTOR, RECOMBINANT HOST MICROORGANISM CELL, METHOD FOR PRODUCING A POLYPEPTIDE, USE OF AT LEAST ONE PROTEASE, METHOD FOR IMPROVING THE NUTRITIONAL VALUE OF AN ANIMAL FEED, ADDITIVE FOR ANIMAL FEED, COMPOSITION OF ANIMAL FEED IMAL, AND, METHOD FOR THE TREATMENT OF EGETAL PROTEINS.
CN102414317B (en) * 2009-02-27 2014-11-05 诺维信公司 Mutant cells having reduced expression of metallopeptidase, suitable for recombinant polypeptide production
EP3080263B1 (en) 2013-12-13 2019-07-03 Danisco US Inc. Serine proteases of the bacillus gibsonii-clade
US20160312204A1 (en) 2013-12-13 2016-10-27 Danisco Us Inc. Serine proteases of bacillus species
US10005850B2 (en) 2013-12-16 2018-06-26 E I Du Pont De Nemours And Company Use of poly alpha-1,3-glucan ethers as viscosity modifiers
US9957334B2 (en) 2013-12-18 2018-05-01 E I Du Pont De Nemours And Company Cationic poly alpha-1,3-glucan ethers
WO2015123323A1 (en) 2014-02-14 2015-08-20 E. I. Du Pont De Nemours And Company Poly-alpha-1,3-1,6-glucans for viscosity modification
EP3116914B8 (en) 2014-03-11 2021-04-21 E. I. du Pont de Nemours and Company Oxidized poly alpha-1,3-glucan as detergent builder
MX2016012044A (en) 2014-03-21 2017-06-29 Danisco Us Inc Serine proteases of bacillus species.
EP3919599A1 (en) 2014-06-19 2021-12-08 Nutrition & Biosciences USA 4, Inc. Compositions containing one or more poly alpha-1,3-glucan ether compounds
US9714403B2 (en) 2014-06-19 2017-07-25 E I Du Pont De Nemours And Company Compositions containing one or more poly alpha-1,3-glucan ether compounds
DK3207129T3 (en) 2014-10-17 2020-02-24 Danisco Us Inc SERIN PROTEAS OF THE BACILLUS ART
DK3212662T3 (en) 2014-10-27 2020-07-20 Danisco Us Inc serine proteases
CN107148472A (en) 2014-10-27 2017-09-08 丹尼斯科美国公司 The serine protease of Bacillus spec
WO2016069569A2 (en) 2014-10-27 2016-05-06 Danisco Us Inc. Serine proteases
EP3212783B1 (en) 2014-10-27 2024-06-26 Danisco US Inc. Serine proteases
EP3212781B1 (en) 2014-10-27 2019-09-18 Danisco US Inc. Serine proteases
CN107109450A (en) 2014-12-23 2017-08-29 纳幕尔杜邦公司 enzymatically produced cellulose
EP4219704A3 (en) 2015-05-13 2023-08-23 Danisco US Inc Aprl-clade protease variants and uses thereof
JP7015695B2 (en) 2015-06-17 2022-02-03 ダニスコ・ユーエス・インク Bacillus gibsonii clade serine protease
CN108603183B (en) 2015-11-05 2023-11-03 丹尼斯科美国公司 Paenibacillus spp. and Bacillus spp. mannanase
EP3371308B1 (en) 2015-11-05 2022-05-11 Danisco US Inc. Paenibacillus sp. mannanases
WO2017083229A1 (en) 2015-11-13 2017-05-18 E. I. Du Pont De Nemours And Company Glucan fiber compositions for use in laundry care and fabric care
WO2017083228A1 (en) 2015-11-13 2017-05-18 E. I. Du Pont De Nemours And Company Glucan fiber compositions for use in laundry care and fabric care
EP3374488B1 (en) 2015-11-13 2020-10-14 DuPont Industrial Biosciences USA, LLC Glucan fiber compositions for use in laundry care and fabric care
US20180362946A1 (en) 2015-12-18 2018-12-20 Danisco Us Inc. Polypeptides with endoglucanase activity and uses thereof
CA3026064A1 (en) 2016-05-31 2017-12-07 Danisco Us Inc. Protease variants and uses thereof
CA3027745A1 (en) 2016-06-17 2017-12-21 Danisco Us Inc. Protease variants and uses thereof
WO2018085524A2 (en) 2016-11-07 2018-05-11 Danisco Us Inc Laundry detergent composition
EP4520820A3 (en) 2016-12-21 2025-04-16 Danisco Us Inc Protease variants and uses thereof
US11946081B2 (en) 2016-12-21 2024-04-02 Danisco Us Inc. Bacillus gibsonii-clade serine proteases
EP3583210B1 (en) 2017-03-15 2021-07-07 Danisco US Inc. Trypsin-like serine proteases and uses thereof
EP3717643A1 (en) 2017-11-29 2020-10-07 Danisco US Inc. Subtilisin variants having improved stability
WO2019245704A1 (en) 2018-06-19 2019-12-26 Danisco Us Inc Subtilisin variants
EP3799601A1 (en) 2018-06-19 2021-04-07 Danisco US Inc. Subtilisin variants
JP2022503923A (en) 2018-09-27 2022-01-12 ダニスコ・ユーエス・インク Composition for cleaning medical equipment
EP3887515A1 (en) 2018-11-28 2021-10-06 Danisco US Inc. Subtilisin variants having improved stability
CN114174504B (en) 2019-05-24 2026-04-17 丹尼斯科美国公司 Subtilis protease variants and usage methods
WO2020247582A1 (en) 2019-06-06 2020-12-10 Danisco Us Inc Methods and compositions for cleaning
US20240034960A1 (en) 2020-08-27 2024-02-01 Danisco Us Inc Enzymes and enzyme compositions for cleaning
EP4284906A1 (en) 2021-01-29 2023-12-06 Danisco US Inc. Compositions for cleaning and methods related thereto
WO2023278297A1 (en) 2021-06-30 2023-01-05 Danisco Us Inc Variant lipases and uses thereof
EP4396320A2 (en) 2021-09-03 2024-07-10 Danisco US Inc. Laundry compositions for cleaning
EP4448750A2 (en) 2021-12-16 2024-10-23 Danisco US Inc. Subtilisin variants and uses thereof
WO2023114939A2 (en) 2021-12-16 2023-06-22 Danisco Us Inc. Subtilisin variants and methods of use
EP4448751A2 (en) 2021-12-16 2024-10-23 Danisco US Inc. Subtilisin variants and methods of use
WO2023168234A1 (en) 2022-03-01 2023-09-07 Danisco Us Inc. Enzymes and enzyme compositions for cleaning
CN119487167A (en) 2022-06-21 2025-02-18 丹尼斯科美国公司 Compositions and methods for cleaning comprising polypeptides having thermolysin activity
EP4581137A1 (en) 2022-09-02 2025-07-09 Danisco Us Inc Mannanase variants and methods of use
CN120112635A (en) 2022-09-02 2025-06-06 丹尼斯科美国公司 Subtilisin variants and methods related thereto
EP4581119A1 (en) 2022-09-02 2025-07-09 Danisco US Inc. Detergent compositions and methods related thereto
CN120303400A (en) 2022-11-09 2025-07-11 丹尼斯科美国公司 Subtilisin variants and methods of use
WO2024163584A1 (en) 2023-02-01 2024-08-08 Danisco Us Inc. Subtilisin variants and methods of use
EP4677080A1 (en) 2023-03-06 2026-01-14 Danisco US Inc. Subtilisin variants and methods of use
CN120813242A (en) 2023-03-16 2025-10-17 营养与生物科学美国第四公司 Brevibacillus brevis ferment extract for cleaning and odor control and uses thereof
WO2025071996A1 (en) 2023-09-28 2025-04-03 Danisco Us Inc. Variant cutinase enzymes with improved solubility and uses thereof
WO2025085351A1 (en) 2023-10-20 2025-04-24 Danisco Us Inc. Subtilisin variants and methods of use
WO2026024921A1 (en) 2024-07-25 2026-01-29 The Procter & Gamble Company Detergent composition comprising a subtilisin variant and methods of use
WO2026050315A1 (en) 2024-08-29 2026-03-05 Danisco Us Inc. Subtilisin variants and methods of use

Citations (2)

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Publication number Priority date Publication date Assignee Title
EP0369817A2 (en) * 1988-11-18 1990-05-23 Omnigene Bioproducts, Inc. Bacillus strains
FR2728905A1 (en) * 1994-12-29 1996-07-05 Rhone Poulenc Nutrition Animal New stereospecific, heat-stable amino acid amidohydrolase

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0369817A2 (en) * 1988-11-18 1990-05-23 Omnigene Bioproducts, Inc. Bacillus strains
FR2728905A1 (en) * 1994-12-29 1996-07-05 Rhone Poulenc Nutrition Animal New stereospecific, heat-stable amino acid amidohydrolase

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL NUCLEOTIDE SEQU 1 January 1900 (1900-01-01), XP002106131, Database accession no. Z99120 *
DATABASE EMBL NUCLEOTIDE SEQU 1 January 1900 (1900-01-01), XP002106132, Database accession no. G70017 *
KUNST F ET AL: "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis", NATURE, vol. 390, 20 November 1997 (1997-11-20), pages 249 - 256, TABLE 1, XP002080813 *

Also Published As

Publication number Publication date
AU1930199A (en) 1999-07-19
GB9727464D0 (en) 1998-02-25
WO1999034003A2 (en) 1999-07-08
EP1042490A2 (en) 2000-10-11

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