WO1999045143A3 - A fluorescence polarization screening method - Google Patents

A fluorescence polarization screening method Download PDF

Info

Publication number
WO1999045143A3
WO1999045143A3 PCT/DK1999/000112 DK9900112W WO9945143A3 WO 1999045143 A3 WO1999045143 A3 WO 1999045143A3 DK 9900112 W DK9900112 W DK 9900112W WO 9945143 A3 WO9945143 A3 WO 9945143A3
Authority
WO
WIPO (PCT)
Prior art keywords
fluorescence polarization
screening method
polarization screening
nucleotide sequence
biological compound
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/DK1999/000112
Other languages
French (fr)
Other versions
WO1999045143A2 (en
Inventor
Lars Kongsbak
Kristian Skovgaard Joergensen
Jesper Valbjoern
Christel Thea Joergensen
Tommy Lykke Husum
Steffen Ernst
Soeren Moeller
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novo Nordisk AS
Original Assignee
Novo Nordisk AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk AS filed Critical Novo Nordisk AS
Priority to DE69900465T priority Critical patent/DE69900465T2/en
Priority to AU26122/99A priority patent/AU2612299A/en
Priority to CA002322488A priority patent/CA2322488A1/en
Priority to DK99906092T priority patent/DK1058738T3/en
Priority to AT99906092T priority patent/ATE208828T1/en
Priority to EP99906092A priority patent/EP1058738B1/en
Priority to JP2000534674A priority patent/JP2002505114A/en
Publication of WO1999045143A2 publication Critical patent/WO1999045143A2/en
Publication of WO1999045143A3 publication Critical patent/WO1999045143A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/40—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6445—Measuring fluorescence polarisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/91045—Acyltransferases (2.3)
    • G01N2333/91074—Aminoacyltransferases (general) (2.3.2)
    • G01N2333/9108—Aminoacyltransferases (general) (2.3.2) with definite EC number (2.3.2.-)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
    • G01N2333/918—Carboxylic ester hydrolases (3.1.1)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Materials By The Use Of Optical Means Adapted For Particular Applications (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A method for screening for a nucleotide sequence encoding for a biological compound comprising measuring the fluorescence polarization of a fluorescent substance reacting with the biological compound expressed by an expression system comprising the nucleotide sequence.
PCT/DK1999/000112 1998-03-06 1999-03-05 A fluorescence polarization screening method Ceased WO1999045143A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
DE69900465T DE69900465T2 (en) 1998-03-06 1999-03-05 A SCREENING PROCEDURE BASED ON FLUORESCENT POLARISATION
AU26122/99A AU2612299A (en) 1998-03-06 1999-03-05 A fluorescence polarization screening method
CA002322488A CA2322488A1 (en) 1998-03-06 1999-03-05 A fluorescence polarization screening method
DK99906092T DK1058738T3 (en) 1998-03-06 1999-03-05 Method of screening based on fluorescence polarization
AT99906092T ATE208828T1 (en) 1998-03-06 1999-03-05 A SCREENING METHOD BASED ON FLUORESCENCE POLARIZATION
EP99906092A EP1058738B1 (en) 1998-03-06 1999-03-05 A fluorescence polarization screening method
JP2000534674A JP2002505114A (en) 1998-03-06 1999-03-05 Fluorescence polarization screening method

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK30898 1998-03-06
DK0308/98 1998-03-06

Publications (2)

Publication Number Publication Date
WO1999045143A2 WO1999045143A2 (en) 1999-09-10
WO1999045143A3 true WO1999045143A3 (en) 1999-10-21

Family

ID=8092089

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK1999/000112 Ceased WO1999045143A2 (en) 1998-03-06 1999-03-05 A fluorescence polarization screening method

Country Status (11)

Country Link
EP (2) EP1156122A3 (en)
JP (1) JP2002505114A (en)
CN (2) CN1560609A (en)
AT (1) ATE208828T1 (en)
AU (1) AU2612299A (en)
CA (1) CA2322488A1 (en)
DE (1) DE69900465T2 (en)
DK (1) DK1058738T3 (en)
ES (1) ES2168848T3 (en)
PT (1) PT1058738E (en)
WO (1) WO1999045143A2 (en)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2328719A1 (en) * 1998-04-15 1999-10-21 Hiroyoshi Hidaka Method for in vivo detecting target protein gene by drug
DE60016951T2 (en) * 1999-10-01 2005-12-15 Novozymes A/S CELLULOSE FILMS FOR PERFORMING SCREENING PROCEDURES
US6426189B1 (en) 1999-10-01 2002-07-30 Novozymes A/S Cellulose films for screening
AU7646100A (en) * 1999-11-05 2001-05-14 Novozymes A/S A high throughput screening (hts) method
US6723537B2 (en) 2001-05-18 2004-04-20 Rigel Pharmaceuticals, Incorporated Directed evolution of protein in mammalian cells
JP4607950B2 (en) * 2004-03-18 2011-01-05 トランス テック ファーマ，インコーポレイテッド Fluorescence polarization assay
FR2897164B1 (en) 2006-02-09 2008-03-14 Commissariat Energie Atomique REALIZING CAVITIES THAT CAN BE FILLED BY A FLUID MATERIAL IN AN OPTICAL MICROTECHNOLOGICAL COMPOUND
FR2929715B1 (en) 2008-04-04 2010-09-10 Commissariat Energie Atomique METHOD FOR PRODUCING MICRONIC OR SUBMICRONIC CAVITIES
FR2933205B1 (en) 2008-06-27 2010-08-13 Commissariat Energie Atomique IMPROVED METHOD FOR MAKING LIQUID-FILLED ENCLOSURES AND CLOSED BY A MEMBRANE
FR2938834B1 (en) * 2008-11-27 2011-03-04 Commissariat Energie Atomique METHOD FOR MAKING A RETENTION MATRIX AND COMPRISING A FUNCTIONAL LIQUID
FR2963112B1 (en) 2010-07-21 2013-02-15 Commissariat Energie Atomique WALL MICROSTRUCTURE WITH DETERMINED OPTICAL PROPERTIES AND METHOD FOR PRODUCING MICROSTRUCTURES
WO2014067933A1 (en) 2012-10-31 2014-05-08 C-Lecta Gmbh Bioactive carrier preparation for enhanced safety in care products and food
KR101494881B1 (en) 2013-05-06 2015-02-24 한국과학기술연구원 Method for detecting Bacillus subtilis using fluorescence probe binding to sulfur oxidoreductase
CN105866316B (en) * 2016-06-24 2018-03-30 曲阜师范大学 It is a kind of while detect the analysis method of amino acid and biogenic amine in food
CN110954517A (en) * 2019-12-11 2020-04-03 西北大学 One-step fluorescence derivatization method of reducing sugar and its application
CN113514445B (en) * 2021-08-05 2023-12-26 荆州市第一人民医院（荆州市肿瘤医院、长江大学附属第一医院） Fluorescence polarization-based deubiquitinase activity detection method
CN114166807B (en) * 2021-11-22 2023-12-12 中国农业大学 Fluorescence polarization immunoassay method for detecting polymyxin
WO2025168975A1 (en) * 2024-02-10 2025-08-14 Biuro Techniczno-Prawne Adam Turczyński Method of manufacturing a probe for determining the activity of pectinolytic enzymes and method of determining the activity of pectinolytic enzymes using said probe
WO2025168976A1 (en) * 2024-02-10 2025-08-14 Biuro Techniczno-Prawne Adam Turczyński Method for detecting pectinolytic pathogens of crops and ornamental plants, microbial medium for use in this method and test kit implementing this method

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0194472A2 (en) * 1985-02-25 1986-09-17 Abbott Laboratories Fluorescence polarization assay for macromolecular hydrolases.
WO1993011249A1 (en) * 1991-12-04 1993-06-10 Novo Nordisk A/S A method of cloning proteins in yeast and cellulase from humicola insolens
EP0639647A2 (en) * 1993-07-08 1995-02-22 Tanabe Seiyaku Co., Ltd. Assay for detecting nucleic acid sequence
WO1997011193A1 (en) * 1995-09-22 1997-03-27 Novo Nordisk A/S Oligosaccharide amino alditols and assay method

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0194472A2 (en) * 1985-02-25 1986-09-17 Abbott Laboratories Fluorescence polarization assay for macromolecular hydrolases.
WO1993011249A1 (en) * 1991-12-04 1993-06-10 Novo Nordisk A/S A method of cloning proteins in yeast and cellulase from humicola insolens
EP0639647A2 (en) * 1993-07-08 1995-02-22 Tanabe Seiyaku Co., Ltd. Assay for detecting nucleic acid sequence
WO1997011193A1 (en) * 1995-09-22 1997-03-27 Novo Nordisk A/S Oligosaccharide amino alditols and assay method

Also Published As

Publication number Publication date
CN1297487A (en) 2001-05-30
EP1156122A3 (en) 2005-02-09
CA2322488A1 (en) 1999-09-10
CN1560609A (en) 2005-01-05
EP1058738B1 (en) 2001-11-14
CN1167810C (en) 2004-09-22
DE69900465T2 (en) 2002-09-05
DE69900465D1 (en) 2001-12-20
JP2002505114A (en) 2002-02-19
WO1999045143A2 (en) 1999-09-10
PT1058738E (en) 2002-05-31
AU2612299A (en) 1999-09-20
ATE208828T1 (en) 2001-11-15
EP1156122A2 (en) 2001-11-21
EP1058738A2 (en) 2000-12-13
ES2168848T3 (en) 2002-06-16
DK1058738T3 (en) 2002-03-11

Similar Documents

Publication Publication Date Title
WO1999045143A3 (en) A fluorescence polarization screening method
AU5429399A (en) Fluorescent assay for biological systems
AU8286498A (en) Raman system for rapid sample identification
AU2779799A (en) Process for producing an electrochemical biosensor
AU2002322518A1 (en) Methods for preparing nucleic acid samples
AU2002217660A1 (en) Substrate for fluorescence analysis
AU3699899A (en) Method for conducting the synthesis of nucleic acid molecules
AU5257201A (en) Method for detecting product of nucleic acid synthesizing reaction
AU2002309998A1 (en) Electrochemical method for high-throughput screening of minute quantities of candidate compounds
AU2996499A (en) High-throughput screening assays for modulators of nucleic acid topoisomerases
AU2789299A (en) Unique identifier for biological samples
AU2002244757A1 (en) Method for specifically detecting, isolating and characterizing cells from body samples by transfecting nucleic acid constructs
AU5999199A (en) Novel antibodies, drugs containing these antibodies and methods for screening compounds by using these antibodies
AU6928700A (en) Identifying organisms by detecting intronic nucleic acid or encoded proteins
ZA9810063B (en) Process for the manufacture of an organic compound.
AU2002223169A1 (en) Method for detecting molecules or chemical reactions by determining variation ofconductance
AU2002317672A1 (en) Method for the biochemical detection of analytes
AU1386599A (en) Methods for identifying biological macromolecule interactions with compounds
AUPS064202A0 (en) Improved method for the analysis of nucleic acid samples
AU6350300A (en) Method for chemical analysis of biological material
AU2000265963A1 (en) Instrument for measuring lifetime of fluorescence
AU3720899A (en) Method for assaying a plurality of analytes
AU8694598A (en) Methods for quantifying nucleic acid molecules
AU7627100A (en) Homogeneous fluorescence method for assaying structural modifications of biomolecules
AU1721001A (en) Method for identifying biochemical material

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 99805151.9

Country of ref document: CN

AK Designated states

Kind code of ref document: A2

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG UZ VN YU ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW SD SL SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

AK Designated states

Kind code of ref document: A3

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG UZ VN YU ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW SD SL SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 1999906092

Country of ref document: EP

ENP Entry into the national phase

Ref document number: 2322488

Country of ref document: CA

Ref document number: 2322488

Country of ref document: CA

Kind code of ref document: A

NENP Non-entry into the national phase

Ref country code: KR

WWP Wipo information: published in national office

Ref document number: 1999906092

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

NENP Non-entry into the national phase

Ref country code: CA

WWG Wipo information: grant in national office

Ref document number: 1999906092

Country of ref document: EP