WO1999053022A3 - Selection of lineage specific cells after differentiation of progenitor cells - Google Patents

Selection of lineage specific cells after differentiation of progenitor cells Download PDF

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Publication number
WO1999053022A3
WO1999053022A3 PCT/GB1999/001136 GB9901136W WO9953022A3 WO 1999053022 A3 WO1999053022 A3 WO 1999053022A3 GB 9901136 W GB9901136 W GB 9901136W WO 9953022 A3 WO9953022 A3 WO 9953022A3
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WO
WIPO (PCT)
Prior art keywords
cells
differentiation
lineage
selection
selected lineage
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB1999/001136
Other languages
French (fr)
Other versions
WO1999053022A2 (en
Inventor
Austin Smith
Meng Li
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Edinburgh
Original Assignee
University of Edinburgh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/535,141 external-priority patent/US6146888A/en
Application filed by University of Edinburgh filed Critical University of Edinburgh
Priority to AT99915938T priority Critical patent/ATE449837T1/en
Priority to CA002325800A priority patent/CA2325800A1/en
Priority to JP2000543570A priority patent/JP2002511246A/en
Priority to EP99915938A priority patent/EP1115840B1/en
Priority to DK99915938.7T priority patent/DK1115840T3/en
Priority to NZ507281A priority patent/NZ507281A/en
Priority to DE69941713T priority patent/DE69941713D1/en
Priority to AU34357/99A priority patent/AU745043B2/en
Priority to IL13893799A priority patent/IL138937A0/en
Publication of WO1999053022A2 publication Critical patent/WO1999053022A2/en
Priority to IL138937A priority patent/IL138937A/en
Priority to US09/686,880 priority patent/US6929948B1/en
Anticipated expiration legal-status Critical
Publication of WO1999053022A3 publication Critical patent/WO1999053022A3/en
Priority to US11/129,333 priority patent/US20060003450A1/en
Priority to US11/620,223 priority patent/US20070264709A1/en
Priority to US11/620,193 priority patent/US7781214B2/en
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0619—Neurons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0622—Glial cells, e.g. astrocytes, oligodendrocytes; Schwann cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0623—Stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/03—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from non-embryonic pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/04—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from germ cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Neurology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Neurosurgery (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

A method for generating a culture that is purified or enriched in respect of cells of a selected lineage is described in which a selectable marker, which is differentially expressed in cells of the selected lineage compared with its expression in other cells, is introduced into a multipotential cell and the multipotential cell is cultured to induce differentiation of the multipotential cell into a cell of the selected lineage or into a mixture of cells including cells of the selected lineage, or is cultured to induce preferential survival of cells of the selected lineage. Those cells that express the selectable marker are then selected for. Progenitors of selected lineage are also described as is the use of the method in assay techniques.
PCT/GB1999/001136 1995-12-29 1999-04-14 Selection of lineage specific cells after differentiation of progenitor cells Ceased WO1999053022A2 (en)

Priority Applications (14)

Application Number Priority Date Filing Date Title
AT99915938T ATE449837T1 (en) 1998-04-14 1999-04-14 LINEAGE-SPECIFIC CELLS AND PROCESSOR CELLS
CA002325800A CA2325800A1 (en) 1998-04-14 1999-04-14 Lineage specific cells and progenitor cells
JP2000543570A JP2002511246A (en) 1998-04-14 1999-04-14 Lineage-specific and progenitor cells
EP99915938A EP1115840B1 (en) 1998-04-14 1999-04-14 Lineage specific cells and progenitor cells
DK99915938.7T DK1115840T3 (en) 1998-04-14 1999-04-14 Progeny-specific cells and progenitor cells
NZ507281A NZ507281A (en) 1998-04-14 1999-04-14 Lineage specific cells and progenitor cells
DE69941713T DE69941713D1 (en) 1998-04-14 1999-04-14 ABSTRACT-SPECIFIC CELLS AND PRECURSOR CELLS
AU34357/99A AU745043B2 (en) 1998-04-14 1999-04-14 Lineage specific cells and progenitor cells
IL13893799A IL138937A0 (en) 1998-04-14 1999-04-14 Lineage specific cells, progenitor cells and methods for generating the same
IL138937A IL138937A (en) 1998-04-14 2000-10-06 Lineage specific cells, progenitor cells and methods for generating the same
US09/686,880 US6929948B1 (en) 1998-04-14 2000-10-12 Lineage specific cells and progenitor cells
US11/129,333 US20060003450A1 (en) 1998-04-14 2005-05-16 Lineage specific cells and phogenitor cells
US11/620,223 US20070264709A1 (en) 1998-04-14 2007-01-05 Lineage specific cells and progenitor cells
US11/620,193 US7781214B2 (en) 1998-04-14 2007-01-05 Lineage specific cells and progenitor cells

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/535,141 US6146888A (en) 1993-04-21 1994-04-21 Method of enriching for mammalian stem cells
GBGB9807935.3A GB9807935D0 (en) 1998-04-14 1998-04-14 Lineage specific cells and progenitor cells
GB9807935.3 1998-04-14

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US09/686,880 Continuation US6929948B1 (en) 1998-04-14 2000-10-12 Lineage specific cells and progenitor cells

Publications (2)

Publication Number Publication Date
WO1999053022A2 WO1999053022A2 (en) 1999-10-21
WO1999053022A3 true WO1999053022A3 (en) 2001-04-19

Family

ID=10830330

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1999/001136 Ceased WO1999053022A2 (en) 1995-12-29 1999-04-14 Selection of lineage specific cells after differentiation of progenitor cells

Country Status (12)

Country Link
US (4) US6929948B1 (en)
EP (1) EP1115840B1 (en)
JP (1) JP2002511246A (en)
AT (1) ATE449837T1 (en)
AU (1) AU745043B2 (en)
CA (1) CA2325800A1 (en)
DE (1) DE69941713D1 (en)
DK (1) DK1115840T3 (en)
GB (1) GB9807935D0 (en)
IL (2) IL138937A0 (en)
NZ (1) NZ507281A (en)
WO (1) WO1999053022A2 (en)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9807935D0 (en) * 1998-04-14 1998-06-10 Univ Edinburgh Lineage specific cells and progenitor cells
US6667176B1 (en) 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
US20050042749A1 (en) 2001-05-16 2005-02-24 Carpenter Melissa K. Dopaminergic neurons and proliferation-competent precursor cells for treating Parkinson's disease
IL152741A0 (en) 2000-05-17 2003-06-24 Geron Corp Neural progenitor cell populations
US7250294B2 (en) 2000-05-17 2007-07-31 Geron Corporation Screening small molecule drugs using neural cells differentiated from human embryonic stem cells
US6576464B2 (en) 2000-11-27 2003-06-10 Geron Corporation Methods for providing differentiated stem cells
WO2002064756A2 (en) * 2001-02-15 2002-08-22 Whitehead Institute For Biomedical Research Stem cell identification
US8524499B2 (en) * 2003-11-10 2013-09-03 Cornell Research Foundation, Inc. Regulatory sequences that direct gene expression to spinal motor neurons and uses thereof
US8673634B2 (en) * 2003-11-13 2014-03-18 Massachusetts Eye & Ear Infirmary Method for the treatment of hearing loss
US20100291042A1 (en) * 2007-05-03 2010-11-18 The Brigham And Women's Hospital, Inc. Multipotent stem cells and uses thereof
US8574567B2 (en) * 2007-05-03 2013-11-05 The Brigham And Women's Hospital, Inc. Multipotent stem cells and uses thereof
DE102008008558A1 (en) * 2008-02-08 2009-08-13 Phenion Gmbh & Co. Kg A method for obtaining olfactory epithelial cells from non-human embryonic stem cells
JP5951254B2 (en) 2008-03-17 2016-07-13 ザ スクリプス リサーチ インスティテュート A combination of chemical and genetic techniques to generate induced pluripotent stem cells
JP6093110B2 (en) 2008-12-17 2017-03-08 ザ スクリプス リサーチ インスティテュート Production and maintenance of stem cells
EP2398897B1 (en) * 2009-02-20 2017-06-28 Cellular Dynamics International, Inc. Methods and compositions for the differentiation of stem cells
JPWO2010137722A1 (en) * 2009-05-26 2012-11-15 武田薬品工業株式会社 Regenerative medicine screening method
US9005968B2 (en) 2009-10-16 2015-04-14 The Scripps Research Institute Induction of pluripotent cells
EP2553086B1 (en) 2010-03-31 2017-04-19 The Scripps Research Institute Reprogramming cells
JP5968871B2 (en) 2010-04-13 2016-08-10 セルラー ダイナミクス インターナショナル， インコーポレイテッド Production of hepatocytes by forward programming
EP3399026B1 (en) 2010-06-14 2024-06-26 The Scripps Research Institute Reprogramming of cells to a new fate
WO2011159684A2 (en) 2010-06-15 2011-12-22 Cellular Dynamics International, Inc. Generation of induced pluripotent stem cells from small volumes of peripheral blood
EP2582792B1 (en) 2010-06-18 2018-10-31 FUJIFILM Cellular Dynamics, Inc. Cardiomyocyte medium with dialyzed serum
WO2012006440A2 (en) 2010-07-07 2012-01-12 Cellular Dynamics International, Inc. Endothelial cell production by programming
US8765470B2 (en) 2010-08-04 2014-07-01 Cellular Dynamics International, Inc. Reprogramming immortalized B-cells to induced pluripotent stem cells
US9181529B2 (en) 2010-10-19 2015-11-10 Cellular Dynamics International, Inc. Titration of differentiation medium components
US9732128B2 (en) * 2010-10-22 2017-08-15 Biotime, Inc. Methods of modifying transcriptional regulatory networks in stem cells
AU2011349446C1 (en) 2010-12-22 2016-01-21 Fate Therapauetics, Inc. Cell culture platform for single cell sorting and enhanced reprogramming of iPSCs
WO2012135621A2 (en) 2011-03-30 2012-10-04 Cellular Dynamics International. Inc Priming of pluripotent stem cells for neural differentiation
WO2013010045A1 (en) 2011-07-12 2013-01-17 Biotime Inc. Novel methods and formulations for orthopedic cell therapy
WO2015073751A1 (en) * 2013-11-15 2015-05-21 Applied Stemcell, Inc. Methods for isolating lineage-specific cells
SG11201606934SA (en) 2014-03-04 2016-09-29 Fate Therapeutics Inc Improved reprogramming methods and cell culture platforms
US10167451B2 (en) 2014-12-22 2019-01-01 The Chinese University Of Hong Kong Combinational use of mechanical manipulation and programin derivatives to increase Oct4, Sox2, or Nanog expression in fibroblasts
CA3001917A1 (en) 2015-10-16 2017-04-20 Fate Therapeutics, Inc. Platform for the induction & maintenance of ground state pluripotency
US11185555B2 (en) 2016-04-11 2021-11-30 Noah James Harrison Method to kill pathogenic microbes in a patient
MX2024001443A (en) 2021-08-11 2024-05-15 Sana Biotechnology Inc Inducible systems for altering gene expression in hypoimmunogenic cells.
GB202308408D0 (en) 2023-06-06 2023-07-19 Cambridge Entpr Ltd Stem cells based three-dimensional embryo model

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993018137A1 (en) * 1992-03-04 1993-09-16 Systemix, Inc. Culturing of hematopoietic stem cells and their genetic engineering
WO1994002593A1 (en) * 1992-07-27 1994-02-03 California Institute Of Technology Mammalian multipotent neural stem cells
WO1997004118A1 (en) * 1995-07-17 1997-02-06 Universite Pierre Et Marie Curie (Paris Vi) Cd4 gene regulatory sequences specifically expressed in mature t cells
WO1999010488A1 (en) * 1997-08-28 1999-03-04 The Salk Institute For Biological Studies Site-specific recombination in eukaryotes and constructs useful therefor

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5364783A (en) * 1990-05-14 1994-11-15 Massachusetts Institute Of Technology Retrovirus promoter-trap vectors
US5654183A (en) * 1992-07-27 1997-08-05 California Institute Of Technology Genetically engineered mammalian neural crest stem cells
US7153684B1 (en) * 1992-10-08 2006-12-26 Vanderbilt University Pluripotential embryonic stem cells and methods of making same
GB9308271D0 (en) * 1993-04-21 1993-06-02 Univ Edinburgh Method of isolating and/or enriching and/or selectively propagating pluripotential animal cells and animals for use in said method
US5602301A (en) * 1993-11-16 1997-02-11 Indiana University Foundation Non-human mammal having a graft and methods of delivering protein to myocardial tissue
US6015671A (en) * 1995-06-07 2000-01-18 Indiana University Foundation Myocardial grafts and cellular compositions
US6001654A (en) * 1994-01-28 1999-12-14 California Institute Of Technology Methods for differentiating neural stem cells to neurons or smooth muscle cells using TGT-β super family growth factors
JPH08322427A (en) * 1995-01-19 1996-12-10 Fujita Gakuen Methods for targeting marked cells and tissues in transgenic animals
GB9505663D0 (en) * 1995-03-21 1995-05-10 Stringer Bradley M J Genetically modified neural cells
US5629145A (en) * 1995-03-24 1997-05-13 Organ, Inc. Cryopreservation of cell suspensions
GB9807935D0 (en) * 1998-04-14 1998-06-10 Univ Edinburgh Lineage specific cells and progenitor cells
US5994075A (en) * 1996-05-17 1999-11-30 Hexagen Technology Limited Methods for identifying a mutation in a gene of interest without a phenotypic guide
US5948623A (en) * 1996-12-31 1999-09-07 Max-Planck Gesellschaft Zur Forderung Der Wissenschaften E.V. Method for testing the differentiation status in pancreatic cells of a mammal

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993018137A1 (en) * 1992-03-04 1993-09-16 Systemix, Inc. Culturing of hematopoietic stem cells and their genetic engineering
WO1994002593A1 (en) * 1992-07-27 1994-02-03 California Institute Of Technology Mammalian multipotent neural stem cells
WO1997004118A1 (en) * 1995-07-17 1997-02-06 Universite Pierre Et Marie Curie (Paris Vi) Cd4 gene regulatory sequences specifically expressed in mature t cells
WO1999010488A1 (en) * 1997-08-28 1999-03-04 The Salk Institute For Biological Studies Site-specific recombination in eukaryotes and constructs useful therefor

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CHENG L ET AL: "Sustained gene expression in retrovirally transduced, engrafting human hematopoietic stem cells and their lympho-myeloid progeny.", BLOOD, (1998 JUL 1) 92 (1) 83-92., XP002112126 *
CONNEALLY E ET AL: "Rapid and efficient selection of human hematopoietic cells expressing murine heat-stable antigen as an indicator of retroviral-mediated gene transfer.", BLOOD, (1996 JAN 15) 87 (2) 456-64. JOURNAL CODE: A8G. ISSN: 0006-4971., XP002112125 *
LI M ET AL: "Generation of purified neural precursors from embryonic stem cells by lineage selection.", CURRENT BIOLOGY, (1998 AUG 27) 8 (17) 971-4. JOURNAL CODE: B44. ISSN: 0960-9822., ENGLAND: United Kingdom, XP002112127 *

Also Published As

Publication number Publication date
US20070264709A1 (en) 2007-11-15
US20070172946A1 (en) 2007-07-26
CA2325800A1 (en) 1999-10-21
US6929948B1 (en) 2005-08-16
NZ507281A (en) 2002-03-28
US7781214B2 (en) 2010-08-24
EP1115840B1 (en) 2009-11-25
US20060003450A1 (en) 2006-01-05
GB9807935D0 (en) 1998-06-10
DK1115840T3 (en) 2010-04-12
ATE449837T1 (en) 2009-12-15
JP2002511246A (en) 2002-04-16
AU745043B2 (en) 2002-03-07
DE69941713D1 (en) 2010-01-07
IL138937A0 (en) 2001-11-25
WO1999053022A2 (en) 1999-10-21
IL138937A (en) 2008-07-08
EP1115840A2 (en) 2001-07-18
AU3435799A (en) 1999-11-01

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