WO1999058652A3 - Cytotoxin-based biological containment - Google Patents

Cytotoxin-based biological containment Download PDF

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Publication number
WO1999058652A3
WO1999058652A3 PCT/DK1999/000258 DK9900258W WO9958652A3 WO 1999058652 A3 WO1999058652 A3 WO 1999058652A3 DK 9900258 W DK9900258 W DK 9900258W WO 9958652 A3 WO9958652 A3 WO 9958652A3
Authority
WO
WIPO (PCT)
Prior art keywords
cytotoxin
gram
polypeptide
cells
organisms
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/DK1999/000258
Other languages
French (fr)
Other versions
WO1999058652A2 (en
Inventor
Kenn Gerdes
Marie Gotfredsen
Hugo Groenlund
Kim Pedersen
Peter Kristoffersen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GX BioSystems AS
Original Assignee
GX BioSystems AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to EP99917812A priority Critical patent/EP1075512B1/en
Priority to DE69942249T priority patent/DE69942249D1/en
Priority to CA2327387A priority patent/CA2327387C/en
Priority to DK99917812.2T priority patent/DK1075512T3/en
Priority to US09/700,130 priority patent/US7183097B1/en
Priority to AU35963/99A priority patent/AU766257C/en
Application filed by GX BioSystems AS filed Critical GX BioSystems AS
Publication of WO1999058652A2 publication Critical patent/WO1999058652A2/en
Publication of WO1999058652A3 publication Critical patent/WO1999058652A3/en
Anticipated expiration legal-status Critical
Priority to US11/558,856 priority patent/US7595185B2/en
Priority to US11/837,456 priority patent/US7595186B2/en
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/68—Stabilisation of the vector

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Method of conditionally controlling the survivability of a recombinant cell population and of containing such cells to an environment or containing replicons to a host cell is based on the use of proteic killer systems including the E. coli relBE locus and similar systems found in Gram-negative and Gram-positive bacteria and Archae. Such system are generally based on a cytotoxin polypeptide and an antitoxin or antidote polypeptide that in contrast to the cytotoxin is degradable by proteases. The recombinant cells are useful as vaccines, pollulant degrading organisms or as biogical pest control organisms e.g. expressing B. thuringiensis crystalline proteins.
PCT/DK1999/000258 1998-05-07 1999-05-07 Cytotoxin-based biological containment Ceased WO1999058652A2 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
DE69942249T DE69942249D1 (en) 1998-05-07 1999-05-07 CYTOTOXIN-BASED BIOLOGICAL INCLUSION SYSTEM
CA2327387A CA2327387C (en) 1998-05-07 1999-05-07 Cytotoxin-based biological containment
DK99917812.2T DK1075512T3 (en) 1998-05-07 1999-05-07 Cytotoxin-based biological encapsulation
US09/700,130 US7183097B1 (en) 1998-05-07 1999-05-07 Cytotoxin-based biological containment
AU35963/99A AU766257C (en) 1998-05-07 1999-05-07 Cytotoxin-based biological containment
EP99917812A EP1075512B1 (en) 1998-05-07 1999-05-07 Cytotoxin-based biological containment
US11/558,856 US7595185B2 (en) 1998-05-07 2006-11-10 Cytotoxin-based biological containment
US11/837,456 US7595186B2 (en) 1998-05-07 2007-08-10 Cytotoxin-based biological containment

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DK0627/98 1998-05-07
DK62798 1998-05-07
US8506798P 1998-05-12 1998-05-12
US60/085,067 1998-05-12

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US09/700,130 A-371-Of-International US7183097B1 (en) 1998-05-07 1999-05-07 Cytotoxin-based biological containment
US11/558,856 Division US7595185B2 (en) 1998-05-07 2006-11-10 Cytotoxin-based biological containment

Publications (2)

Publication Number Publication Date
WO1999058652A2 WO1999058652A2 (en) 1999-11-18
WO1999058652A3 true WO1999058652A3 (en) 2000-01-20

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK1999/000258 Ceased WO1999058652A2 (en) 1998-05-07 1999-05-07 Cytotoxin-based biological containment

Country Status (7)

Country Link
US (3) US7183097B1 (en)
EP (2) EP1075512B1 (en)
AU (1) AU766257C (en)
CA (1) CA2327387C (en)
DE (1) DE69942249D1 (en)
DK (1) DK1075512T3 (en)
WO (1) WO1999058652A2 (en)

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US7625707B2 (en) 2003-10-02 2009-12-01 Ramot At Tel Aviv University Ltd. Antibacterial agents and methods of identifying and utilizing same
US8927689B2 (en) 2002-12-09 2015-01-06 Ramot At Tel-Aviv University Ltd. Peptide nanostructures and methods of generating and using the same
US9394628B2 (en) 2004-08-02 2016-07-19 Ramot At Tel-Aviv University Ltd. Method of forming a fiber made of peptide nanostructures
US11458172B2 (en) 2017-09-08 2022-10-04 New Portal Limited Nucleic acid systems that enable bacteria to specifically target solid tumors via glucose-dependent viability
US12491236B2 (en) 2020-07-07 2025-12-09 New Portal Limited Genetically engineered live bacteria and methods of constructing the same

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Cited By (7)

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Publication number Priority date Publication date Assignee Title
US8927689B2 (en) 2002-12-09 2015-01-06 Ramot At Tel-Aviv University Ltd. Peptide nanostructures and methods of generating and using the same
US7625707B2 (en) 2003-10-02 2009-12-01 Ramot At Tel Aviv University Ltd. Antibacterial agents and methods of identifying and utilizing same
US9394628B2 (en) 2004-08-02 2016-07-19 Ramot At Tel-Aviv University Ltd. Method of forming a fiber made of peptide nanostructures
US11458172B2 (en) 2017-09-08 2022-10-04 New Portal Limited Nucleic acid systems that enable bacteria to specifically target solid tumors via glucose-dependent viability
US11696931B2 (en) 2017-09-08 2023-07-11 New Portal Limited Bacteria for targeting tumors and treating cancer
US12042517B2 (en) 2017-09-08 2024-07-23 New Portal Limited Nucleic acid systems that enable bacteria to specifically target solid tumors via glucose-dependent viability
US12491236B2 (en) 2020-07-07 2025-12-09 New Portal Limited Genetically engineered live bacteria and methods of constructing the same

Also Published As

Publication number Publication date
CA2327387A1 (en) 1999-11-08
US20080182327A1 (en) 2008-07-31
EP1075512A2 (en) 2001-02-14
DE69942249D1 (en) 2010-05-27
US7183097B1 (en) 2007-02-27
US7595186B2 (en) 2009-09-29
DK1075512T3 (en) 2010-06-07
EP2180050A2 (en) 2010-04-28
AU3596399A (en) 1999-11-29
WO1999058652A2 (en) 1999-11-18
AU766257C (en) 2004-06-03
US20080206840A1 (en) 2008-08-28
US7595185B2 (en) 2009-09-29
EP2180050B1 (en) 2016-04-20
EP2180050A3 (en) 2010-06-30
CA2327387C (en) 2012-07-03
EP1075512B1 (en) 2010-04-14
AU766257B2 (en) 2003-10-09

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