WO1999058948A3 - Enumeration method of analyte detection - Google Patents

Enumeration method of analyte detection Download PDF

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Publication number
WO1999058948A3
WO1999058948A3 PCT/US1999/010917 US9910917W WO9958948A3 WO 1999058948 A3 WO1999058948 A3 WO 1999058948A3 US 9910917 W US9910917 W US 9910917W WO 9958948 A3 WO9958948 A3 WO 9958948A3
Authority
WO
WIPO (PCT)
Prior art keywords
signal
sample
analyte
enumeration
binding events
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1999/010917
Other languages
French (fr)
Other versions
WO1999058948A2 (en
Inventor
Timothy W Starzl
Scott Clark
Marybeth Robinson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DDX Inc
Original Assignee
DDX Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DDX Inc filed Critical DDX Inc
Priority to JP2000548701A priority Critical patent/JP2002526743A/en
Priority to EP99925655A priority patent/EP1188059A2/en
Priority to AU41900/99A priority patent/AU4190099A/en
Priority to IL13963199A priority patent/IL139631A0/en
Priority to CA002331787A priority patent/CA2331787A1/en
Priority to NZ508062A priority patent/NZ508062A/en
Publication of WO1999058948A2 publication Critical patent/WO1999058948A2/en
Anticipated expiration legal-status Critical
Publication of WO1999058948A3 publication Critical patent/WO1999058948A3/en
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites

Landscapes

  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Nanotechnology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Composite Materials (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Materials Engineering (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

This invention is directed to an optically-based method and system for analyte detection using solid phase immobilization, specific analyte labels adapted for signal generation and corresponding processes for the utilization thereof. The enumeration detection method disclosed herein narrows the area for signal observation, thus, improving detectable signal to background ratio. The system is comprised of a platform/support for immobilizing a sample stage having a labeled sample (analyte complex) bound thereto, a radiation source, an optical apparatus for generating and directing radiation at said sample and a means for data collection and analysis. Upon engagement of the system, the sample generates a signal capable of differentiation from background signal, both of which are collected and imaged with a signal detector that generated a sample image to a data processing apparatus. Said apparatus receives signal measurements and, in turn, enumerates individual binding events. Generated signal may be increased via selected mass enhancement. The invention, enumeration assay methodology detecting individual binding events, may be used, for example, in analyses to detect analyte or confirm results in both research, commercial and point of care applications.
PCT/US1999/010917 1998-05-13 1999-05-13 Enumeration method of analyte detection Ceased WO1999058948A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
JP2000548701A JP2002526743A (en) 1998-05-13 1999-05-13 Analyte detection calculation method
EP99925655A EP1188059A2 (en) 1998-05-13 1999-05-13 Enumeration method of analyte detection
AU41900/99A AU4190099A (en) 1998-05-13 1999-05-13 Enumeration method of analyte detection
IL13963199A IL139631A0 (en) 1998-05-13 1999-05-13 Enumeration method of analyte detection
CA002331787A CA2331787A1 (en) 1998-05-13 1999-05-13 Enumeration method of analyte detection
NZ508062A NZ508062A (en) 1998-05-13 1999-05-13 Enumeration method of analyte detection

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US8525998P 1998-05-13 1998-05-13
US60/085,259 1998-05-13

Publications (2)

Publication Number Publication Date
WO1999058948A2 WO1999058948A2 (en) 1999-11-18
WO1999058948A3 true WO1999058948A3 (en) 2002-01-03

Family

ID=22190457

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/010917 Ceased WO1999058948A2 (en) 1998-05-13 1999-05-13 Enumeration method of analyte detection

Country Status (7)

Country Link
EP (1) EP1188059A2 (en)
JP (1) JP2002526743A (en)
AU (1) AU4190099A (en)
CA (1) CA2331787A1 (en)
IL (1) IL139631A0 (en)
NZ (1) NZ508062A (en)
WO (1) WO1999058948A2 (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8895255B1 (en) 2003-07-12 2014-11-25 Accelerate Diagnostics, Inc. Sensitive and rapid determination of antimicrobial susceptibility
US9057046B2 (en) 2005-09-26 2015-06-16 Rapid Micro Biosystems, Inc. Cassette containing growth medium
US9290382B2 (en) 2001-09-06 2016-03-22 Rapid Micro Biosystems Rapid detection of replicating cells
US9434937B2 (en) 2011-03-07 2016-09-06 Accelerate Diagnostics, Inc. Rapid cell purification systems

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60130970T2 (en) * 2000-01-31 2008-07-31 Matsushita Ecology Systems Co., Ltd., Kasugai REAGENT SET FOR DETECTING MICROORGANISMS, APPARATUS FOR QUANTIFYING MICROORGANISMS AND METHOD FOR QUANTIFYING MICROORGANISMS
GB0029154D0 (en) * 2000-11-30 2001-01-17 Lee Helen Signal enhancement with multiple labelled-antibodies
DE10113711A1 (en) * 2001-03-16 2002-09-26 Lifebits Ag Identifying bio-chemical reactions e.g., DNA hybridization, comprises digitally scanning specific detector molecules in an array of accurately-defined and discrete points with a sample, for statistical evaluation
JP3945636B2 (en) * 2001-03-27 2007-07-18 富士フイルム株式会社 measuring device
US20120077206A1 (en) 2003-07-12 2012-03-29 Accelr8 Technology Corporation Rapid Microbial Detection and Antimicrobial Susceptibility Testing
US8492098B2 (en) 2006-02-21 2013-07-23 The Trustees Of Tufts College Methods and arrays for target analyte detection and determination of reaction components that affect a reaction
US11237171B2 (en) 2006-02-21 2022-02-01 Trustees Of Tufts College Methods and arrays for target analyte detection and determination of target analyte concentration in solution
US20110195852A1 (en) * 2007-08-30 2011-08-11 Trustees Of Tufts College Methods for determining the concentration of an analyte in solution
US8222047B2 (en) 2008-09-23 2012-07-17 Quanterix Corporation Ultra-sensitive detection of molecules on single molecule arrays
AU2009296526B2 (en) 2008-09-24 2015-11-19 First Light Diagnostics, Inc. Method for detecting analytes
WO2011073867A1 (en) 2009-12-18 2011-06-23 Koninklijke Philips Electronics N.V. Substance determining apparatus
US8236574B2 (en) 2010-03-01 2012-08-07 Quanterix Corporation Ultra-sensitive detection of molecules or particles using beads or other capture objects
WO2011109379A1 (en) 2010-03-01 2011-09-09 Quanterix Corporation Methods and systems for extending dynamic range in assays for the detection of molecules or particles
US9678068B2 (en) 2010-03-01 2017-06-13 Quanterix Corporation Ultra-sensitive detection of molecules using dual detection methods
US8415171B2 (en) 2010-03-01 2013-04-09 Quanterix Corporation Methods and systems for extending dynamic range in assays for the detection of molecules or particles
US9952237B2 (en) 2011-01-28 2018-04-24 Quanterix Corporation Systems, devices, and methods for ultra-sensitive detection of molecules or particles
WO2012117332A1 (en) * 2011-02-28 2012-09-07 Koninklijke Philips Electronics N.V. Substance determining apparatus
US10254204B2 (en) 2011-03-07 2019-04-09 Accelerate Diagnostics, Inc. Membrane-assisted purification
WO2012142301A2 (en) 2011-04-12 2012-10-18 Quanterix Corporation Methods of determining a treatment protocol for and/or a prognosis of a patients recovery from a brain injury
DK3327110T3 (en) 2011-11-07 2020-04-27 Rapid Micro Biosystems Inc Kassette til sterilitetstest
EP2839021B1 (en) 2012-04-16 2022-02-23 Rapid Micro Biosystems, Inc. Cell culturing device
US9932626B2 (en) 2013-01-15 2018-04-03 Quanterix Corporation Detection of DNA or RNA using single molecule arrays and other techniques
US9677109B2 (en) 2013-03-15 2017-06-13 Accelerate Diagnostics, Inc. Rapid determination of microbial growth and antimicrobial susceptibility
US10253355B2 (en) 2015-03-30 2019-04-09 Accelerate Diagnostics, Inc. Instrument and system for rapid microorganism identification and antimicrobial agent susceptibility testing
AU2016243656A1 (en) 2015-03-30 2017-11-09 Accelerate Diagnostics, Inc. Instrument and system for rapid microorganism identification and antimicrobial agent susceptibility testing
CN119804399A (en) 2018-04-19 2025-04-11 曙光诊断学公司 Target Detection
AU2019353145B2 (en) 2018-10-04 2025-12-04 First Light Diagnostics, Inc. Microbial analysis without cell purification

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4508832A (en) * 1981-06-22 1985-04-02 Battelle Memorial Institute Ellipsometrically measuring rate of optical change in immunoassay
US4521522A (en) * 1981-09-05 1985-06-04 Kurt I. Lundstroem Optical specific binding assay with reflection of polarized electromagnetic radiation
US4805623A (en) * 1987-09-04 1989-02-21 Vander Corporation Spectrophotometric method for quantitatively determining the concentration of a dilute component in a light- or other radiation-scattering environment
US4876208A (en) * 1987-01-30 1989-10-24 Yellowstone Diagnostics Corporation Diffraction immunoassay apparatus and method
US4885077A (en) * 1988-11-17 1989-12-05 Becton, Dickinson And Company Composite membrane, method for its preparation and electrolyte sensor including same
US5017009A (en) * 1986-06-26 1991-05-21 Ortho Diagnostic Systems, Inc. Scattered total internal reflectance immunoassay system
US5496701A (en) * 1991-06-04 1996-03-05 Fisons Plc Optical biosensor method for determining an analyte
US5622868A (en) * 1989-04-27 1997-04-22 Microbiological Research Authority Camr (Centre For Applied Microbiology & Research) Analytical apparatus utilizing a colorimetric or other optically detectable effect

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4508832A (en) * 1981-06-22 1985-04-02 Battelle Memorial Institute Ellipsometrically measuring rate of optical change in immunoassay
US4521522A (en) * 1981-09-05 1985-06-04 Kurt I. Lundstroem Optical specific binding assay with reflection of polarized electromagnetic radiation
US5017009A (en) * 1986-06-26 1991-05-21 Ortho Diagnostic Systems, Inc. Scattered total internal reflectance immunoassay system
US4876208A (en) * 1987-01-30 1989-10-24 Yellowstone Diagnostics Corporation Diffraction immunoassay apparatus and method
US4805623A (en) * 1987-09-04 1989-02-21 Vander Corporation Spectrophotometric method for quantitatively determining the concentration of a dilute component in a light- or other radiation-scattering environment
US4885077A (en) * 1988-11-17 1989-12-05 Becton, Dickinson And Company Composite membrane, method for its preparation and electrolyte sensor including same
US5622868A (en) * 1989-04-27 1997-04-22 Microbiological Research Authority Camr (Centre For Applied Microbiology & Research) Analytical apparatus utilizing a colorimetric or other optically detectable effect
US5496701A (en) * 1991-06-04 1996-03-05 Fisons Plc Optical biosensor method for determining an analyte

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9290382B2 (en) 2001-09-06 2016-03-22 Rapid Micro Biosystems Rapid detection of replicating cells
US8895255B1 (en) 2003-07-12 2014-11-25 Accelerate Diagnostics, Inc. Sensitive and rapid determination of antimicrobial susceptibility
US9057046B2 (en) 2005-09-26 2015-06-16 Rapid Micro Biosystems, Inc. Cassette containing growth medium
US9434937B2 (en) 2011-03-07 2016-09-06 Accelerate Diagnostics, Inc. Rapid cell purification systems

Also Published As

Publication number Publication date
JP2002526743A (en) 2002-08-20
CA2331787A1 (en) 1999-11-18
AU4190099A (en) 1999-11-29
EP1188059A2 (en) 2002-03-20
IL139631A0 (en) 2002-02-10
NZ508062A (en) 2002-04-26
WO1999058948A2 (en) 1999-11-18

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