WO1999065586A3 - Expanded bed adsorption system - Google Patents

Expanded bed adsorption system Download PDF

Info

Publication number
WO1999065586A3
WO1999065586A3 PCT/DK1999/000336 DK9900336W WO9965586A3 WO 1999065586 A3 WO1999065586 A3 WO 1999065586A3 DK 9900336 W DK9900336 W DK 9900336W WO 9965586 A3 WO9965586 A3 WO 9965586A3
Authority
WO
WIPO (PCT)
Prior art keywords
eba
columns
expanded bed
outlet
expanded
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/DK1999/000336
Other languages
French (fr)
Other versions
WO1999065586A2 (en
Inventor
Rene Oehlenschlaeger
Brian A Olsen
Elias Zafirakos
Allan Otto Fog Lihme
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Upfront Chromatography AS
Original Assignee
Upfront Chromatography AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Upfront Chromatography AS filed Critical Upfront Chromatography AS
Priority to US09/719,781 priority Critical patent/US6620326B1/en
Priority to EP99927730A priority patent/EP1087828B1/en
Priority to DE69939590T priority patent/DE69939590D1/en
Priority to AU44984/99A priority patent/AU4498499A/en
Publication of WO1999065586A2 publication Critical patent/WO1999065586A2/en
Publication of WO1999065586A3 publication Critical patent/WO1999065586A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1807—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using counter-currents, e.g. fluidised beds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/45—Magnetic mixers; Mixers with magnetically driven stirrers
    • B01F33/452—Magnetic mixers; Mixers with magnetically driven stirrers using independent floating stirring elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3204—Inorganic carriers, supports or substrates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3268—Macromolecular compounds
    • B01J20/3272—Polymers obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
    • B01J20/3274—Proteins, nucleic acids, polysaccharides, antibodies or antigens
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2220/00—Aspects relating to sorbent materials
    • B01J2220/50—Aspects relating to the use of sorbent or filter aid materials
    • B01J2220/52—Sorbents specially adapted for preparative chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2220/00—Aspects relating to sorbent materials
    • B01J2220/50—Aspects relating to the use of sorbent or filter aid materials
    • B01J2220/58—Use in a single column
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/28—Control of physical parameters of the fluid carrier
    • G01N30/32—Control of physical parameters of the fluid carrier of pressure or speed
    • G01N2030/324—Control of physical parameters of the fluid carrier of pressure or speed speed, flow rate
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6004—Construction of the column end pieces
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6052—Construction of the column body
    • G01N30/6082—Construction of the column body transparent to radiation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Inorganic Chemistry (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • External Artificial Organs (AREA)

Abstract

The present application describes various improvements and further developments to the Expanded Bed Adsorption (EBA) technology. In particular, the application describes an EBA process where elution is performed in fluidised mode, i.e. in expanded bed mode, the so-called All Expanded Process. This improvement which is based on the use of controlled density particles, e.g. particles having a density of at least 1.3 g/ml, provides valuable advantages with respect to use of reduced amounts of elution buffer. The application also describes the use of magnetic stirring in EBA columns, design of an inlet ring with multiple openings, outlet at the bottom or side an EBA column for the discharge of gel, mobile pipe outlet, floating outlet, temperature regulator at inlet, the use of coloured gel, the use of sensors, processes for the regeneration of buffer in EBA systems, the introduction of baffles, and disposable columns (single use sterile columns).
PCT/DK1999/000336 1998-06-18 1999-06-18 Expanded bed adsorption system Ceased WO1999065586A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
US09/719,781 US6620326B1 (en) 1998-06-18 1999-06-18 Expanded bed adsorption system
EP99927730A EP1087828B1 (en) 1998-06-18 1999-06-18 Expanded bed adsorption system
DE69939590T DE69939590D1 (en) 1998-06-18 1999-06-18 EXPANDED BED ABSORPTION SYSTEM
AU44984/99A AU4498499A (en) 1998-06-18 1999-06-18 Expanded bed adsorption system

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA199800828 1998-06-18
DK82898 1998-06-18

Publications (2)

Publication Number Publication Date
WO1999065586A2 WO1999065586A2 (en) 1999-12-23
WO1999065586A3 true WO1999065586A3 (en) 2000-02-24

Family

ID=8098012

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK1999/000336 Ceased WO1999065586A2 (en) 1998-06-18 1999-06-18 Expanded bed adsorption system

Country Status (5)

Country Link
US (1) US6620326B1 (en)
EP (1) EP1087828B1 (en)
AU (1) AU4498499A (en)
DE (1) DE69939590D1 (en)
WO (1) WO1999065586A2 (en)

Families Citing this family (69)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1289641A2 (en) * 2000-05-12 2003-03-12 Upfront Chromatography A/S A bed adsorption system
SE0100714D0 (en) * 2000-07-13 2001-02-28 Ap Biotech Ab Reaction vessel and method for distributing fluid in such a vessel
DE10035292A1 (en) * 2000-07-18 2002-02-21 Mpb Cologne Gmbh Molecular Pla Process for obtaining proteins from plants in pure form
SE0100071D0 (en) * 2001-01-11 2001-01-11 Apbiotech Ab Continuous separation method
EP1584242B1 (en) 2001-06-01 2010-01-20 Upfront Chromatography A/S Fractionation of protein containing mixtures
DK1605772T3 (en) * 2003-03-21 2007-10-29 Upfront Chromatography As Process for high throughput volumes in the fractionation of biomolecules by chromatographic systems
US20070199899A1 (en) * 2004-03-31 2007-08-30 Andrew Alaska Sweep-flow methods and clogging disrupters, for expanded bed chromatography of liquids with suspended particulates
EP1765866B1 (en) 2004-06-07 2014-01-08 Therapure Biopharma Inc. Isolation of plasma or serum proteins
USD551104S1 (en) * 2006-09-21 2007-09-18 Ge Healthcare Bio-Sciences Ab Chromatography column
CN101631599A (en) * 2007-01-15 2010-01-20 厄普弗朗特色谱公司 Production of biofuels and proteins from feedstock
WO2008092450A1 (en) * 2007-01-29 2008-08-07 Upfront Chromatography A/S Isolation and separation of minimally denatured potato proteins and peptides
EP1972933A1 (en) * 2007-03-23 2008-09-24 Coöperatie AVEBE U.A. Chromatographic column system
AU2013201133B2 (en) * 2007-03-28 2014-02-27 Patheon Holdings I B.V. Expanded bed column and disposable chromatography
CA2682333C (en) 2007-03-28 2015-05-05 Upfront Chromatography A/S Expanded bed column and disposable chromatography
DK1994972T3 (en) * 2007-05-15 2011-10-17 Cooperatie Avebe U A Process and apparatus for continuous chromatographic separation
CN101690854B (en) * 2009-09-22 2011-08-31 周鑫 Novel expanded bed
WO2011152781A1 (en) * 2010-05-31 2011-12-08 Ge Healthcare Bio-Sciences Ab Adjustable volume mixer chamber and method of use
US10463988B2 (en) 2010-09-23 2019-11-05 Ge Healthcare Bio-Sciences Corp. Single use slurrying and chromatography systems
CN102120102B (en) * 2010-11-30 2013-02-27 顾雄毅 Expanded bed chromatographic separation device for biochemical separation technology
CN103429349B (en) * 2011-01-28 2015-09-16 株式会社日冷生物科学 The rabbling mechanism of elongated liquid in container, method
MY180565A (en) * 2011-04-14 2020-12-02 Gs Caltex Corp Apparatus and method for separating and refining product manufactured by microbial fermentation by using adsorbent
JP5634339B2 (en) * 2011-06-27 2014-12-03 孝昭 石井 Chromatographic separation method
US9351978B2 (en) 2012-02-29 2016-05-31 Mead Johnson Nutrition Company Neurogenesis screening method and uses thereof
IN2015DN02055A (en) 2012-09-17 2015-08-14 Grace W R & Co
WO2014044746A1 (en) * 2012-09-24 2014-03-27 Thermo Electron Manufacturing Limited Improvements in and relating to chromatography columns
GB201302714D0 (en) * 2013-02-15 2013-04-03 Biotechflow Ltd Chromatography systems and processes
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US20180161292A1 (en) 2016-12-12 2018-06-14 Mead Johnson Nutrition Company Nutritional compositions containing butyrate and uses thereof
US20180168215A1 (en) 2016-12-21 2018-06-21 Mead Johnson Nutrition Company Nutritional compositions containing inositol and uses thereof
WO2018139145A1 (en) * 2017-01-30 2018-08-02 東レ株式会社 Purification column
US20180333426A1 (en) 2017-05-17 2018-11-22 Mead Johnson Nutrition Company Nutritional composition with human milk oligosaccharides and uses thereof
US20180332881A1 (en) 2017-05-17 2018-11-22 Mead Johnson Nutrition Company Preterm infant formula containing butyrate and uses thereof
US20190208807A1 (en) 2018-01-05 2019-07-11 Mead Johnson Nutrition Company Nutritional compositions containing milk-derived peptides and uses thereof
GB2573538B (en) 2018-05-09 2023-01-04 Mjn Us Holdings Llc Pediatric nutritional compositions and methods for infants delivered by C-section
GB202203613D0 (en) * 2022-03-16 2022-04-27 Biotechflow Ltd Expanded bed chromatography apparatus
CN115920515A (en) * 2022-12-26 2023-04-07 浙江嘉邻信息科技有限公司 Efficient adjustable water purifier

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4780113A (en) * 1987-10-16 1988-10-25 Exxon Chemical Patents Inc. Isomobility focusing in a magnetically stabilized fluidized bed
DK165090D0 (en) 1990-07-09 1990-07-09 Kem En Tec As CONLOMERATED PARTICLES
SE9101149D0 (en) * 1991-04-17 1991-04-17 Pharmacia Lkb Biotech BEADS FOR DOWN STREAM PROCESSING
SE9400263L (en) * 1994-01-28 1995-07-29 Pharmacia Biotech Ab column Shelf
DE29611336U1 (en) * 1996-06-28 1996-09-19 Gesellschaft für Biotechnologische Forschung mbH (GBF), 38124 Braunschweig Filtration device
JP2000042303A (en) * 1998-07-30 2000-02-15 Takenori Tanimura Multistage liquid-solid extractor

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BJORKLUND M ET AL: "Characterisation of silica-based heparin-affinity adsorbents through column chromatography of plasma fractions containing thrombin<1", JOURNAL OF CHROMATOGRAPHY A, vol. 743, no. 1, 30 August 1996 (1996-08-30), pages 145-162, XP004020279, ISSN: 0021-9673 *
HJORTH R: "Expanded-bed adsorption in industrial bioprocessing: recent developments", TRENDS IN BIOTECHNOLOGY, vol. 15, no. 6, 1 June 1997 (1997-06-01), pages 230-235, XP004064512, ISSN: 0167-7799 *
KAUFMANN M: "Unstable proteins: how to subject them to chromatographic separations for purification procedures", JOURNAL OF CHROMATOGRAPHY B: BIOMEDICAL SCIENCES & APPLICATIONS, vol. 699, no. 1-2, 10 October 1997 (1997-10-10), pages 347-369, XP004095003, ISSN: 0378-4347 *
THOMMES J ET AL: "Isolation of monoclonal antibodies from cell containing hybridoma broth using a protein A coated adsorbent in expanded beds", JOURNAL OF CHROMATOGRAPHY A, vol. 752, no. 1-2, 1 November 1996 (1996-11-01), pages 111-122, XP004071206, ISSN: 0021-9673 *

Also Published As

Publication number Publication date
EP1087828B1 (en) 2008-09-17
EP1087828A2 (en) 2001-04-04
WO1999065586A2 (en) 1999-12-23
AU4498499A (en) 2000-01-05
DE69939590D1 (en) 2008-10-30
US6620326B1 (en) 2003-09-16

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