WO2000003683A3 - Liposomal encapsulated nucleic acid-complexes - Google Patents

Liposomal encapsulated nucleic acid-complexes Download PDF

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Publication number
WO2000003683A3
WO2000003683A3 PCT/US1999/016166 US9916166W WO0003683A3 WO 2000003683 A3 WO2000003683 A3 WO 2000003683A3 US 9916166 W US9916166 W US 9916166W WO 0003683 A3 WO0003683 A3 WO 0003683A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
complexes
encapsulated nucleic
liposomes
liposomal encapsulated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1999/016166
Other languages
French (fr)
Other versions
WO2000003683A2 (en
Inventor
Anthony Boey
Tao Chen
Esther Leng
Paul G Tard
Jeffrey J Wheeler
Peter Scherrer
Roger Graham
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Inex Pharmaceuticals Corp
Original Assignee
Inex Pharmaceuticals Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inex Pharmaceuticals Corp filed Critical Inex Pharmaceuticals Corp
Priority to DE69906977T priority Critical patent/DE69906977T2/en
Priority to AT99934114T priority patent/ATE237312T1/en
Priority to AU50019/99A priority patent/AU747597B2/en
Priority to CA002335393A priority patent/CA2335393C/en
Priority to EP99934114A priority patent/EP1096921B1/en
Priority to JP2000559818A priority patent/JP2002520038A/en
Publication of WO2000003683A2 publication Critical patent/WO2000003683A2/en
Publication of WO2000003683A3 publication Critical patent/WO2000003683A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/88—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/127—Synthetic bilayered vehicles, e.g. liposomes or liposomes with cholesterol as the only non-phosphatidyl surfactant
    • A61K9/1271—Non-conventional liposomes, e.g. PEGylated liposomes or liposomes coated or grafted with polymers
    • A61K9/1272—Non-conventional liposomes, e.g. PEGylated liposomes or liposomes coated or grafted with polymers comprising non-phosphatidyl surfactants as bilayer-forming substances, e.g. cationic lipids or non-phosphatidyl liposomes coated or grafted with polymers

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Organic Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Preparation (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

This invention relates to liposomes which are useful for the introduction of nucleic acids into cells. The liposomes of the present invention entrap a condensing agent-nucleic acid complex and are suitable for nucleic acid-transfer delivery vehicles in clinical use. In addition, methods of transfecting a cell with a nucleic acid using the liposomes of the present invention are also disclosed.
PCT/US1999/016166 1998-07-20 1999-07-16 Liposomal encapsulated nucleic acid-complexes Ceased WO2000003683A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
DE69906977T DE69906977T2 (en) 1998-07-20 1999-07-16 NUCLEIC ACID COMPLEXES ENCLOSED IN LIPOSOMES
AT99934114T ATE237312T1 (en) 1998-07-20 1999-07-16 NUCLEIC ACID COMPLEXES ENCAPSULATED IN LIPOSOMES
AU50019/99A AU747597B2 (en) 1998-07-20 1999-07-16 Liposomal encapsulated nucleic acid-complexes
CA002335393A CA2335393C (en) 1998-07-20 1999-07-16 Liposomal encapsulated nucleic acid-complexes
EP99934114A EP1096921B1 (en) 1998-07-20 1999-07-16 Liposomal encapsulated nucleic acid-complexes
JP2000559818A JP2002520038A (en) 1998-07-20 1999-07-16 Liposome encapsulated nucleic acid complex

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9348198P 1998-07-20 1998-07-20
US60/093,481 1998-07-20

Publications (2)

Publication Number Publication Date
WO2000003683A2 WO2000003683A2 (en) 2000-01-27
WO2000003683A3 true WO2000003683A3 (en) 2000-04-27

Family

ID=22239187

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/016166 Ceased WO2000003683A2 (en) 1998-07-20 1999-07-16 Liposomal encapsulated nucleic acid-complexes

Country Status (7)

Country Link
EP (1) EP1096921B1 (en)
JP (1) JP2002520038A (en)
AT (1) ATE237312T1 (en)
AU (1) AU747597B2 (en)
CA (1) CA2335393C (en)
DE (1) DE69906977T2 (en)
WO (1) WO2000003683A2 (en)

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US9018187B2 (en) 2009-07-01 2015-04-28 Protiva Biotherapeutics, Inc. Cationic lipids and methods for the delivery of therapeutic agents
US9035039B2 (en) 2011-12-22 2015-05-19 Protiva Biotherapeutics, Inc. Compositions and methods for silencing SMAD4
US11845925B2 (en) 2010-07-06 2023-12-19 Glaxosmithkline Biologicals Sa Immunisation of large mammals with low doses of RNA
US11850305B2 (en) 2010-07-06 2023-12-26 Glaxosmithkline Biologicals Sa Method of making lipid formulations with RNA encoding immunogens
US11932854B2 (en) 2010-10-29 2024-03-19 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using short interfering nucleic acids (siNA)

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