WO2000020112A3 - Chemical constructs and their uses - Google Patents

Chemical constructs and their uses Download PDF

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Publication number
WO2000020112A3
WO2000020112A3 PCT/GB1999/003284 GB9903284W WO0020112A3 WO 2000020112 A3 WO2000020112 A3 WO 2000020112A3 GB 9903284 W GB9903284 W GB 9903284W WO 0020112 A3 WO0020112 A3 WO 0020112A3
Authority
WO
WIPO (PCT)
Prior art keywords
cleavage site
fragment
groups
cleavable
chemical
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB1999/003284
Other languages
French (fr)
Other versions
WO2000020112A2 (en
Inventor
Stephen Carl Mckeown
Stephen Paul Watson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Glaxo Group Ltd
Original Assignee
Glaxo Group Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Glaxo Group Ltd filed Critical Glaxo Group Ltd
Priority to AT99947748T priority Critical patent/ATE222884T1/en
Priority to DE69902678T priority patent/DE69902678T2/en
Priority to JP2000573466A priority patent/JP2002526753A/en
Priority to EP99947748A priority patent/EP1119528B1/en
Priority to AU61120/99A priority patent/AU6112099A/en
Priority to DK99947748T priority patent/DK1119528T3/en
Publication of WO2000020112A2 publication Critical patent/WO2000020112A2/en
Publication of WO2000020112A3 publication Critical patent/WO2000020112A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/1072—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups
    • C07K1/1077—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups by covalent attachment of residues other than amino acids or peptide residues, e.g. sugars, polyols, fatty acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D239/00—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings
    • C07D239/02—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings
    • C07D239/24—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings having three or more double bonds between ring members or between ring members and non-ring members
    • C07D239/28—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings having three or more double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, directly attached to ring carbon atoms
    • C07D239/32—One oxygen, sulfur or nitrogen atom
    • C07D239/38—One sulfur atom
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C07K1/042—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers characterised by the nature of the carrier
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/0054—Means for coding or tagging the apparatus or the reagents
    • B01J2219/00572—Chemical means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00702—Processes involving means for analysing and characterising the products
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00725—Peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B2200/00—Indexing scheme relating to specific properties of organic compounds
    • C07B2200/11—Compounds covalently bound to a solid support
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/10—Libraries containing peptides or polypeptides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B70/00—Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Materials For Medical Uses (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention provides a chemical construct for use in solid phase synthesis comprising a solid support Q having linked thereto groups Y?1R and Y2¿R; wherein R is a substrate or a coding tag and the groups Y?1 and Y2¿ are connecting groups each having a first cleavage site, at least one of Y?1 and Y2¿ having a second cleavage site located between the first cleavage site and group R, the first cleavage site being orthogonally and selectively cleavable with respect to the second cleavage site, and, when both groups Y?1 and Y2¿ contain a second cleavage site, the second cleavage site in Y1 being selectively and orthogonally cleavable with respect to the second cleavage site in Y2; the second cleavage site being cleavable to release the substrate; and the first cleavage site being selectively cleavable to release a fragment Fr comprising the substrate R and at least a portion of the connecting group Y; and wherein: (i) the chemical fragment Fr contains a sensitising group G which sensitises the chemical fragment Fr to instrumental, e.g. mass spectroscopic analysis; and/or (ii) the fragment Fr contains a means for imparting a characteristic signature to the mass spectrum of the fragment.
PCT/GB1999/003284 1998-10-05 1999-10-05 Chemical constructs and their uses Ceased WO2000020112A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
AT99947748T ATE222884T1 (en) 1998-10-05 1999-10-05 CHEMICAL CONSTRUCTIONS AND THEIR USES
DE69902678T DE69902678T2 (en) 1998-10-05 1999-10-05 CHEMICAL CONSTRUCTIONS AND THEIR USE
JP2000573466A JP2002526753A (en) 1998-10-05 1999-10-05 Chemical constructs and their use
EP99947748A EP1119528B1 (en) 1998-10-05 1999-10-05 Chemical constructs and their uses
AU61120/99A AU6112099A (en) 1998-10-05 1999-10-05 Chemical constructs and their uses
DK99947748T DK1119528T3 (en) 1998-10-05 1999-10-05 Chemical constructions and their applications

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9821669.0 1998-10-05
GBGB9821669.0A GB9821669D0 (en) 1998-10-05 1998-10-05 Chemical constructs and their uses

Publications (2)

Publication Number Publication Date
WO2000020112A2 WO2000020112A2 (en) 2000-04-13
WO2000020112A3 true WO2000020112A3 (en) 2000-10-26

Family

ID=10840013

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1999/003284 Ceased WO2000020112A2 (en) 1998-10-05 1999-10-05 Chemical constructs and their uses

Country Status (10)

Country Link
EP (1) EP1119528B1 (en)
JP (1) JP2002526753A (en)
AT (1) ATE222884T1 (en)
AU (1) AU6112099A (en)
DE (1) DE69902678T2 (en)
DK (1) DK1119528T3 (en)
ES (1) ES2181478T3 (en)
GB (1) GB9821669D0 (en)
PT (1) PT1119528E (en)
WO (1) WO2000020112A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002538163A (en) 1999-03-05 2002-11-12 マサチューセッツ インスティテュート オブ テクノロジー Linkers for oligosaccharide synthesis on solid supports
ATE344456T1 (en) * 2000-05-05 2006-11-15 Purdue Research Foundation AFFINITY-SELECTED SIGNATURE EPPTIDES FOR IDENTIFYING AND QUANTIFYING PROTEINS
US6787635B2 (en) * 2001-04-05 2004-09-07 3M Innovative Properties Company Solid phase synthesis supports and methods
DE10117274B4 (en) * 2001-04-06 2005-03-03 Hte Ag The High Throughput Experimentation Company Method for analyzing and archiving at least one material library
JP4613299B2 (en) 2003-01-30 2011-01-12 ディーエイチ テクノロジーズ デベロップメント プライベート リミテッド Methods, mixtures, kits, and compositions for analyte analysis
US20050148087A1 (en) 2004-01-05 2005-07-07 Applera Corporation Isobarically labeled analytes and fragment ions derived therefrom
US20070048752A1 (en) 2004-07-12 2007-03-01 Applera Corporation Mass tags for quantitative analyses
CA2801064A1 (en) * 2010-06-01 2011-12-08 Advanced Proteome Therapeutics Inc. Crosslinking of proteins and other entities via conjugates of alpha-haloacetophenones, benzyl halides, quinones, and their derivatives

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995028640A1 (en) * 1994-04-13 1995-10-26 The Trustees Of Columbia University In The City Of New York Complex combinatorial chemical libraries encoded with tags
WO1997037953A1 (en) * 1996-04-08 1997-10-16 Glaxo Group Ltd. Mass-based encoding and qualitative analysis of combinatorial libraries

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995028640A1 (en) * 1994-04-13 1995-10-26 The Trustees Of Columbia University In The City Of New York Complex combinatorial chemical libraries encoded with tags
WO1997037953A1 (en) * 1996-04-08 1997-10-16 Glaxo Group Ltd. Mass-based encoding and qualitative analysis of combinatorial libraries

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CARRASCO M R ET AL: "Direct Monitoring of Organic Reactions on Polymeric Supports", TETRAHEDRON LETTERS,NL,ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, vol. 38, no. 36, 8 September 1997 (1997-09-08), pages 6331 - 6334, XP004087929, ISSN: 0040-4039 *
GEYSEN H M ET AL: "ISOTOPE OR MASS ENCODING OF COMBINATORIAL LIBRARIES", CHEMISTRY AND BIOLOGY,GB,CURRENT BIOLOGY, LONDON, vol. 3, no. 8, 1 August 1996 (1996-08-01), pages 679 - 688, XP002035873, ISSN: 1074-5521 *

Also Published As

Publication number Publication date
ATE222884T1 (en) 2002-09-15
EP1119528A2 (en) 2001-08-01
PT1119528E (en) 2002-12-31
ES2181478T3 (en) 2003-02-16
AU6112099A (en) 2000-04-26
GB9821669D0 (en) 1998-12-02
WO2000020112A2 (en) 2000-04-13
EP1119528B1 (en) 2002-08-28
DE69902678T2 (en) 2003-04-24
DK1119528T3 (en) 2002-12-30
JP2002526753A (en) 2002-08-20
DE69902678D1 (en) 2002-10-02

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