WO2000020609A3 - Protective recombinant haemophilus influenzae high molecular weight proteins - Google Patents

Protective recombinant haemophilus influenzae high molecular weight proteins Download PDF

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Publication number
WO2000020609A3
WO2000020609A3 PCT/CA1999/000938 CA9900938W WO0020609A3 WO 2000020609 A3 WO2000020609 A3 WO 2000020609A3 CA 9900938 W CA9900938 W CA 9900938W WO 0020609 A3 WO0020609 A3 WO 0020609A3
Authority
WO
WIPO (PCT)
Prior art keywords
operon
proteins
molecular weight
high molecular
coli
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/CA1999/000938
Other languages
French (fr)
Other versions
WO2000020609A2 (en
Inventor
Sheena M Loosmore
Yan-Ping Yang
Michel H Klein
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sanofi Pasteur Ltd
Original Assignee
Connaught Laboratories Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/206,942 external-priority patent/US6432669B1/en
Priority to CA2345208A priority Critical patent/CA2345208C/en
Priority to NZ511360A priority patent/NZ511360A/en
Priority to JP2000574704A priority patent/JP3654512B2/en
Priority to AU60736/99A priority patent/AU765339B2/en
Priority to AT99947153T priority patent/ATE474927T1/en
Application filed by Connaught Laboratories Ltd filed Critical Connaught Laboratories Ltd
Priority to EP99947153A priority patent/EP1117807B1/en
Priority to DE69942611T priority patent/DE69942611D1/en
Publication of WO2000020609A2 publication Critical patent/WO2000020609A2/en
Publication of WO2000020609A3 publication Critical patent/WO2000020609A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/285—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pasteurellaceae (F), e.g. Haemophilus influenza
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Communicable Diseases (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Public Health (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Physics & Mathematics (AREA)
  • Veterinary Medicine (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicinal Preparation (AREA)

Abstract

Protective high molecular weight (HMW) proteins are produced recombinantly by expression from E. coli by using a promoter effective in E. coli and a nucleic acid molecule which contains a modified operon of a non-typeable strain of Haemophilus. The modified operon contains the portion only of the A region which encodes the mature HMW protein and the complete B and C regions of the operon. Enhanced levels of expression of the HMW proteins can be achieved by including the E. coli cer gene, a further copy of the portion of the A region of the operon encoding the mature protein or both, in the expression vector. Nucleotide and deduced amino acid sequences of the hmw1 and hmw2 genes and HMW1 and HMW2 proteins, respectively, of several non-typeable Haemophilus influenzae strain have been identified.
PCT/CA1999/000938 1998-10-07 1999-10-07 Protective recombinant haemophilus influenzae high molecular weight proteins Ceased WO2000020609A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
DE69942611T DE69942611D1 (en) 1998-10-07 1999-10-07 PROTECTIVE HIGH-MOLECULAR WEIGHT PROTEINS FROM HAEMOPHILUS INFLUENZAE
NZ511360A NZ511360A (en) 1998-10-07 1999-10-07 Protective recombinant haemophilus influenzae high molecular weight proteins
JP2000574704A JP3654512B2 (en) 1998-10-07 1999-10-07 Nucleic acid molecule encoding transformation recombinant HAEMOPHILUSINFLUENZAE (H. influenzae) high molecular weight protein, transformation vector, transformed strain and method for producing protein using them
AU60736/99A AU765339B2 (en) 1998-10-07 1999-10-07 Protective recombinant (haemophilus influenzae) high molecular weight proteins
AT99947153T ATE474927T1 (en) 1998-10-07 1999-10-07 PROTECTIVE HIGH MOLECULAR WEIGHT PROTEINS FROM HAEMOPHILUS INFLUENZAE
CA2345208A CA2345208C (en) 1998-10-07 1999-10-07 Protective recombinant haemophilus influenzae high molecular weight proteins
EP99947153A EP1117807B1 (en) 1998-10-07 1999-10-07 Protective recombinant haemophilus influenzae high molecular weight proteins

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US16756898A 1998-10-07 1998-10-07
US09/167,568 1998-10-07
US09/206,942 US6432669B1 (en) 1998-10-07 1998-12-08 Protective recombinant Haemophilus influenzae high molecular weight proteins
US09/206,942 1998-12-08

Publications (2)

Publication Number Publication Date
WO2000020609A2 WO2000020609A2 (en) 2000-04-13
WO2000020609A3 true WO2000020609A3 (en) 2000-08-03

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA1999/000938 Ceased WO2000020609A2 (en) 1998-10-07 1999-10-07 Protective recombinant haemophilus influenzae high molecular weight proteins

Country Status (6)

Country Link
EP (1) EP1117807B1 (en)
JP (2) JP3654512B2 (en)
AU (1) AU765339B2 (en)
CA (1) CA2345208C (en)
NZ (1) NZ511360A (en)
WO (1) WO2000020609A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101103103A (en) * 2004-11-19 2008-01-09 威斯康星旧生研究基金会 Recombinant influenza virus vectors with tandem transcription units

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0502637A2 (en) * 1991-02-26 1992-09-09 Zeneca Limited Vector
WO1993019090A1 (en) * 1992-03-16 1993-09-30 Barenkamp Stephen J High molecular weight surface proteines of non-typeable haemophilus
WO1994021290A1 (en) * 1993-03-16 1994-09-29 Barenkamp Stephen J High molecular weight surface proteins of non-typeable haemophilus
WO1997036914A1 (en) * 1996-04-01 1997-10-09 Barenkamp Stephen J High molecular weight surface proteins of non-typeable haemophilus

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0502637A2 (en) * 1991-02-26 1992-09-09 Zeneca Limited Vector
WO1993019090A1 (en) * 1992-03-16 1993-09-30 Barenkamp Stephen J High molecular weight surface proteines of non-typeable haemophilus
WO1994021290A1 (en) * 1993-03-16 1994-09-29 Barenkamp Stephen J High molecular weight surface proteins of non-typeable haemophilus
WO1997036914A1 (en) * 1996-04-01 1997-10-09 Barenkamp Stephen J High molecular weight surface proteins of non-typeable haemophilus

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BARENKAMP S J ET AL: "GENES ENCODING HIGH-MOLECULAR-WEIGHT ADHESION PROTEINS OF NONTYPEABLE HAEMOPHILUS INFLUENZAE ARE PART OF GENE CLUSTERS", INFECTION AND IMMUNITY,US,AMERICAN SOCIETY FOR MICROBIOLOGY. WASHINGTON, vol. 62, no. 8, 1 August 1994 (1994-08-01), pages 3320 - 3328, XP000578342, ISSN: 0019-9567 *
BARENKAMP S.J.: "Immunization with High-Molecular-Weight adhesion proteins of nontypeable Haemophilus influenzae modifies experimental otitis media in chinchillas", INF. IMMUN., vol. 64, no. 4, April 1996 (1996-04-01), pages 1245 - 1251, XP002133637 *
DAWID S.R. ET AL.: "High Molecular Weight adhesins of nontypeable Haemophilus influenzae: characterization of a novel mechanism of regulation", ABSTRACTS OF THE 1998 GENERAL MEETING OF THE AMERICAN SOCIETY FOR MICROBIOLOGY, vol. 98, 17 May 1998 (1998-05-17), pages 74, XP002133638 *
ST. GEME III J.W. ET AL.: "Prevalence and distribution of the hmw and hia genes and the HMW and Hia adhesins among genetically diverse strains of nontypeable Haemophilus influenzae", INFECTION AND IMMUNITY, vol. 66, no. 1, January 1998 (1998-01-01), pages 364 - 368, XP002137980 *
ST. GEME III, J.W. ET AL.: "Secretion of the Haemophilus influenzae HMW1 and HMW2 adhesins involves a periplasmic intermediate and requires the HMWB and HMWC proteins.", MOL. MICROBIOL., vol. 27, no. 3, February 1998 (1998-02-01), pages 617 - 630, XP000892544 *

Also Published As

Publication number Publication date
JP4151844B2 (en) 2008-09-17
CA2345208A1 (en) 2000-04-13
JP2002532062A (en) 2002-10-02
AU6073699A (en) 2000-04-26
AU765339B2 (en) 2003-09-18
EP1117807B1 (en) 2010-07-21
JP2004166710A (en) 2004-06-17
JP3654512B2 (en) 2005-06-02
NZ511360A (en) 2004-01-30
CA2345208C (en) 2012-01-17
WO2000020609A2 (en) 2000-04-13
EP1117807A2 (en) 2001-07-25

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