WO2000028007A3 - Chemically modified mutant serine hydrolases - Google Patents

Chemically modified mutant serine hydrolases

Info

Publication number
WO2000028007A3
WO2000028007A3 PCT/US1999/026586 US9926586W WO0028007A3 WO 2000028007 A3 WO2000028007 A3 WO 2000028007A3 US 9926586 W US9926586 W US 9926586W WO 0028007 A3 WO0028007 A3 WO 0028007A3
Authority
WO
WIPO (PCT)
Prior art keywords
serine hydrolases
cysteine
chemically modified
group
modified
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1999/026586
Other languages
French (fr)
Other versions
WO2000028007A2 (en
Inventor
John Bryan Jones
Michael Dickman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Genencor International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor International Inc filed Critical Genencor International Inc
Priority to DE69940978T priority Critical patent/DE69940978D1/en
Priority to JP2000581174A priority patent/JP4932990B2/en
Priority to AU30990/00A priority patent/AU772427B2/en
Priority to EP99964973A priority patent/EP1129180B1/en
Priority to DK99964973T priority patent/DK1129180T3/en
Priority to AT99964973T priority patent/ATE433485T1/en
Priority to CA002348014A priority patent/CA2348014A1/en
Publication of WO2000028007A2 publication Critical patent/WO2000028007A2/en
Publication of WO2000028007A3 publication Critical patent/WO2000028007A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

This invention provides chemically modified mutant serine hydrolases that catalyze a transamidation and/or a transpeptidation and/or a transesterification reaction. The modified serine hydrolases have one or more amino acid residues in a subsite replaced with a cysteine, wherein the cysteine is modified by replacing the thiol hydrogen in the cysteine with a substituent group providing a thiol side chain comprising a moiety selected from the group consisting of a polar aromatic substituent, an alkyl amino group with a positive charge, and a glycoside. In particularly preferred embodiments, the substitutents include an oxazolidinone, a C1 to C15 alkyl amino group with a positive charge, or a glycoside.
PCT/US1999/026586 1998-11-10 1999-11-09 Chemically modified mutant serine hydrolases Ceased WO2000028007A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
DE69940978T DE69940978D1 (en) 1998-11-10 1999-11-09 CHEMICALLY MODIFIED MUTANT SERINHYDROLASES
JP2000581174A JP4932990B2 (en) 1998-11-10 1999-11-09 Chemically modified mutant serine hydrolase exhibits improved catalytic activity and chiral selectivity
AU30990/00A AU772427B2 (en) 1998-11-10 1999-11-09 Chemically modified mutant serine hydrolases show improved catalytic activity and chiral selectivity
EP99964973A EP1129180B1 (en) 1998-11-10 1999-11-09 Chemically modified mutant serine hydrolases
DK99964973T DK1129180T3 (en) 1998-11-10 1999-11-09 Chemically Modified Mutant Serine Hydrolases
AT99964973T ATE433485T1 (en) 1998-11-10 1999-11-09 CHEMICALLY MODIFIED MUTANT SERINE HYDROLASES
CA002348014A CA2348014A1 (en) 1998-11-10 1999-11-09 Chemically modified mutant serine hydrolases

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US10775898P 1998-11-10 1998-11-10
US60/107,758 1998-11-10
US11306198P 1998-12-21 1998-12-21
US60/113,061 1998-12-21

Publications (2)

Publication Number Publication Date
WO2000028007A2 WO2000028007A2 (en) 2000-05-18
WO2000028007A3 true WO2000028007A3 (en) 2000-07-27

Family

ID=26805118

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/026586 Ceased WO2000028007A2 (en) 1998-11-10 1999-11-09 Chemically modified mutant serine hydrolases

Country Status (9)

Country Link
US (4) US20050282248A1 (en)
EP (1) EP1129180B1 (en)
JP (1) JP4932990B2 (en)
AT (1) ATE433485T1 (en)
AU (1) AU772427B2 (en)
CA (1) CA2348014A1 (en)
DE (1) DE69940978D1 (en)
DK (1) DK1129180T3 (en)
WO (1) WO2000028007A2 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6908757B1 (en) 1998-03-26 2005-06-21 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
US6495136B1 (en) 1998-03-26 2002-12-17 The Procter & Gamble Company Proteases having modified amino acid sequences conjugated to addition moieties
US6569663B1 (en) 1998-03-26 2003-05-27 The Procter & Gamble Company Serine protease variants having amino acid substitutions
WO2000028007A2 (en) 1998-11-10 2000-05-18 Genencor International, Inc. Chemically modified mutant serine hydrolases
EP1185302B1 (en) * 1999-04-28 2013-07-24 Genencor International, Inc. Specifically targeted catalytic antagonists and uses thereof
BR0012660A (en) 1999-07-22 2002-04-09 Procter & Gamble Protease variant of subtilisin type; cleaning composition; and personal care composition
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
CN1373802A (en) 1999-07-22 2002-10-09 宝洁公司 Subtilisin variants with amino acid deletions and substitutions in specific epitope regions
EP1196547A2 (en) * 1999-07-22 2002-04-17 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
MXPA02000842A (en) 1999-07-22 2002-07-30 Procter & Gamble Protease conjugates having sterically protected clip sites.
US7202070B2 (en) * 2000-10-31 2007-04-10 Biocatalytics, Inc. Method for reductive amination of a ketone using a mutated enzyme
DE10121463A1 (en) * 2001-05-02 2003-02-27 Henkel Kgaa New alkaline protease variants and washing and cleaning agents containing these new alkaline protease variants
EP1354960A1 (en) * 2002-04-08 2003-10-22 Degussa AG Assay for synthesis activity of hydrolases
EP1359225B1 (en) * 2002-04-08 2006-11-15 Degussa AG Assay for synthesis activity of hydrolases
EP3307848B1 (en) 2015-06-10 2019-03-06 Merck Patent GmbH Chiral dopants having a nortricyclan unit

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998023732A2 (en) * 1996-11-26 1998-06-04 Genencor International, Inc. Chemically modified enzymes
WO1999037323A1 (en) * 1998-01-23 1999-07-29 Genencor International, Inc. Chemically modified mutant enzymes and methods for producing them and screening them
WO1999037324A1 (en) * 1998-01-23 1999-07-29 Genencor International, Inc. Modified enzymes and their use for peptide synthesis
WO2000001712A2 (en) * 1998-07-02 2000-01-13 Genencor International, Inc. Chemically modified proteins with a carbohydrate moiety

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5155033A (en) 1989-01-06 1992-10-13 Genencor, Inc. Subtilisins modified at position 225 resulting in a shift in catalytic activity
US5972682A (en) 1984-05-29 1999-10-26 Genencor International, Inc. Enzymatically active modified subtilisins
US5244791A (en) 1984-05-29 1993-09-14 Genecor International, Inc. Methods of ester hydrolysis
CN1056187C (en) 1988-02-11 2000-09-06 金克克国际有限公司 Proteolytic enzymes and their use in detergents
US5116741A (en) * 1988-04-12 1992-05-26 Genex Corporation Biosynthetic uses of thermostable proteases
DK97190D0 (en) 1990-04-19 1990-04-19 Novo Nordisk As OXIDATION STABLE DETERGENT ENZYMER
EP0542931B1 (en) 1990-08-09 1999-12-08 Genentech, Inc. Serine protease variants having peptide ligase activity
US5629173A (en) * 1990-08-09 1997-05-13 Genentech, Inc. Methods of use of serine protease variants having peptide ligase activity
US5316935A (en) * 1992-04-06 1994-05-31 California Institute Of Technology Subtilisin variants suitable for hydrolysis and synthesis in organic media
US5837516A (en) 1995-03-03 1998-11-17 Genentech, Inc. Subtilisin variants capable of cleaving substrates containing basic residues
US6946280B1 (en) 1996-03-29 2005-09-20 Genencor International, Inc. Enzyme multimer and process of producing same
AR015977A1 (en) 1997-10-23 2001-05-30 Genencor Int PROTEASA VARIANTS MULTIPLY SUBSTITUTED WITH ALTERED NET LOAD FOR USE IN DETERGENTS
WO2000028007A2 (en) 1998-11-10 2000-05-18 Genencor International, Inc. Chemically modified mutant serine hydrolases
AU2200100A (en) * 1998-12-21 2000-07-12 Genencor International, Inc. Chemically modified enzymes with multiple charged variants

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998023732A2 (en) * 1996-11-26 1998-06-04 Genencor International, Inc. Chemically modified enzymes
WO1999037323A1 (en) * 1998-01-23 1999-07-29 Genencor International, Inc. Chemically modified mutant enzymes and methods for producing them and screening them
WO1999037324A1 (en) * 1998-01-23 1999-07-29 Genencor International, Inc. Modified enzymes and their use for peptide synthesis
WO2000001712A2 (en) * 1998-07-02 2000-01-13 Genencor International, Inc. Chemically modified proteins with a carbohydrate moiety

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BECH L M ET AL: "CHEMICAL MODIFICATIONS OF A CYSTEINYL RESIDUE INTRODUCED IN THE BINDING SITE OF CARBOXYPEPTIDASE Y BY SITE-DIRECTED MUTAGENESIS", CARLSBERG RESEARCH COMMUNICATIONS,XX,XX, vol. 53, 1 January 1988 (1988-01-01), pages 381 - 393, XP002063095, ISSN: 0105-1938 *
BERGLUND P ET AL: "CHEMICAL MODIFICAITON OF CYSTEINE MUTANTS OF SUBTILISIN BACILLUS LENTUS CAN CREATE BETTER CATALYSTS THAN THE WILD-TYPE ENZYME", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,US,AMERICAN CHEMICAL SOCIETY, WASHINGTON, DC, vol. 119, no. 22, 4 June 1997 (1997-06-04), pages 5265 - 5266, XP002063097, ISSN: 0002-7863 *
DAVIS, BENJAMIN G. ET AL: "Controlled Site-Selective Glycosylation of Proteins by a Combined Site-Directed Mutagenesis and Chemical Modification Approach", J. ORG. CHEM. (1998), 63(26), 9614-9615, XP002135378 *
DESANTIS G ET AL: "Site-directed mutagenesis combined with chemical modification as a strategy for altering the specificity of the S1 and S1' pockets of subtilisin Bacillus lentus.", BIOCHEMISTRY, (1998 APR 28) 37 (17) 5968-73., XP002135377 *
DICKMAN, MICHAEL ET AL: "Chemically modified mutants of subtilisin Bacillus lentus catalyze transesterification reactions better than wild type", TETRAHEDRON: ASYMMETRY (1998), 9(23), 4099-4102, XP000891276 *

Also Published As

Publication number Publication date
US20120156721A1 (en) 2012-06-21
CA2348014A1 (en) 2000-05-18
US8357524B2 (en) 2013-01-22
ATE433485T1 (en) 2009-06-15
AU3099000A (en) 2000-05-29
JP2002529078A (en) 2002-09-10
AU772427B2 (en) 2004-04-29
DE69940978D1 (en) 2009-07-23
JP4932990B2 (en) 2012-05-16
US20090075329A1 (en) 2009-03-19
US8148128B2 (en) 2012-04-03
EP1129180A2 (en) 2001-09-05
DK1129180T3 (en) 2009-08-24
US20120295329A1 (en) 2012-11-22
US20050282248A1 (en) 2005-12-22
EP1129180B1 (en) 2009-06-10
WO2000028007A2 (en) 2000-05-18

Similar Documents

Publication Publication Date Title
WO2000028007A3 (en) Chemically modified mutant serine hydrolases
Beckett et al. Glutathione S-transferases: biomedical applications
WO1995030744A3 (en) Lipases with improved surfactant resistance
BR9811192A (en) Enzymatic cleaning compositions
WO2003006652A3 (en) Inhibition of specific histone deacetylase isoforms
AU8623198A (en) Antimicrobial composition containing a haloperoxidase, a hydrogen peroxide source, a halide source and an ammonium source
WO2001079458A3 (en) Polypeptides having haloperoxidase activity
AU2001253404A1 (en) Process of disinfecting a hard-surface with a composition comprising cinnamon oil and/or an active thereof
WO2004020458A3 (en) Assays and compositions for identifying agents that modulate the activity of deubiquitinating agents
WO1998023732A3 (en) Chemically modified enzymes
AU5815296A (en) Determination of glycated proteins
WO2001092473A3 (en) Method for cultivating a cartilage replacement and a biomatrix produced according to this method
EP1141507A4 (en) Hinge structure
WO1998038285A3 (en) Mutant carboxypeptidase y and methods for transamidation
AU1166199A (en) Methods for identifying the toxic/pathologic effect of environmental stimuli on gene transcription
PL343841A1 (en) Substance system
WO2004027382A3 (en) Sensor assemblies and methods of securing elongated members within such assemblies
AU2002242858A1 (en) Patch-clamping and its use in analysing subcellular features
AU5897696A (en) Use of mannanases as slime control agents
EP1179183A4 (en) A method for identifying pre-neoplastic and/or neoplastic states in mammals
AU2002304335A1 (en) Culture medium for enhanced heterologous protein expression
GB0115382D0 (en) Mutant
WO2001012204A3 (en) Xanthine oxidase inhibitor containing compositions for the treatment of acetaminophen intoxication
GB2366767A (en) Improvements in or relating to marking instruments
AU1740500A (en) Thyroid peroxidase autoantibody assay compositions, method and kit

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
ENP Entry into the national phase

Ref document number: 2348014

Country of ref document: CA

Ref country code: CA

Ref document number: 2348014

Kind code of ref document: A

Format of ref document f/p: F

ENP Entry into the national phase

Ref country code: JP

Ref document number: 2000 581174

Kind code of ref document: A

Format of ref document f/p: F

WWE Wipo information: entry into national phase

Ref document number: 30990/00

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 1999964973

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 1999964973

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWG Wipo information: grant in national office

Ref document number: 30990/00

Country of ref document: AU