WO2000028053A3 - Plant glutamine amidotransferase homologs - Google Patents

Plant glutamine amidotransferase homologs Download PDF

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Publication number
WO2000028053A3
WO2000028053A3 PCT/US1999/025950 US9925950W WO0028053A3 WO 2000028053 A3 WO2000028053 A3 WO 2000028053A3 US 9925950 W US9925950 W US 9925950W WO 0028053 A3 WO0028053 A3 WO 0028053A3
Authority
WO
WIPO (PCT)
Prior art keywords
homologs
biosynthetic enzyme
glutamine amidotransferase
histidine biosynthetic
relates
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1999/025950
Other languages
French (fr)
Other versions
WO2000028053A2 (en
Inventor
Stephen M Allen
Lisa L Huang
Saverio Carl Falco
Antoni J Rafalski
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EIDP Inc
Original Assignee
EI Du Pont de Nemours and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co
Priority to EP99956903A priority Critical patent/EP1127144A2/en
Priority to AU13413/00A priority patent/AU1341300A/en
Priority to US09/831,233 priority patent/US6855867B1/en
Publication of WO2000028053A2 publication Critical patent/WO2000028053A2/en
Publication of WO2000028053A3 publication Critical patent/WO2000028053A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

This invention relates to an isolated nucleic acid fragment encoding a histidine biosynthetic enzyme. The invention also relates to the construction of a chimeric gene encoding all or a portion of the histidine biosynthetic enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the histidine biosynthetic enzyme in a transformed host cell.
PCT/US1999/025950 1998-11-05 1999-11-04 Plant glutamine amidotransferase homologs Ceased WO2000028053A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP99956903A EP1127144A2 (en) 1998-11-05 1999-11-04 Plant glutamine amidotransferase homologs
AU13413/00A AU1341300A (en) 1998-11-05 1999-11-04 Plant glutamine amidotransferase homologs
US09/831,233 US6855867B1 (en) 1998-11-05 1999-11-04 Plant glutamine amidotransferase homologs

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10727598P 1998-11-05 1998-11-05
US60/107,275 1998-11-05

Publications (2)

Publication Number Publication Date
WO2000028053A2 WO2000028053A2 (en) 2000-05-18
WO2000028053A3 true WO2000028053A3 (en) 2000-08-17

Family

ID=22315811

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/025950 Ceased WO2000028053A2 (en) 1998-11-05 1999-11-04 Plant glutamine amidotransferase homologs

Country Status (3)

Country Link
EP (1) EP1127144A2 (en)
AU (1) AU1341300A (en)
WO (1) WO2000028053A2 (en)

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DATABASE EMEST14 EMBL Heidelberg, Germany; 28 July 1999 (1999-07-28), SHOEMAKER R ET AL.: "Glycine max cDNA clone similar to: glutamine amidotransferase/cyclase", XP002136030 *
DATABASE EMEST21 EMBL Heidelberg, Germany; 18 October 1999 (1999-10-18), WALBOT V: "Maize ESTs from various cDNA libraries sequenced at Stanford University", XP002136029 *
DATABASE NEW_TREMBL EMBL Heidelberg, Germany; 17 June 1999 (1999-06-17), BEVAN M ET AL.: "Glutamine amidotransferase/cyclase", XP002136028 *
FUJIMORI K AND OHTA D: "An Arabidopsis cDNA encoding a bifunctional glutamine amidotransferase/cyclase suppresses the histidine auxotrophy of a Saccharomyces cerevisiae his7 mutant", FEBS LETTERS, vol. 428, no. 3, 29 May 1998 (1998-05-29), pages 229 - 234, XP002136027 *
KLEM T J AND DAVISSON V J: "Imidazole glycerole phosphate synthase: the glutamine amidotransferase in histidine biosynthesis", BIOCHEMISTRY, vol. 32, 1993, pages 5177 - 5186, XP002136052 *

Also Published As

Publication number Publication date
AU1341300A (en) 2000-05-29
EP1127144A2 (en) 2001-08-29
WO2000028053A2 (en) 2000-05-18

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