WO2000028071A3 - A method for the determination of homocysteine - Google Patents

A method for the determination of homocysteine Download PDF

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Publication number
WO2000028071A3
WO2000028071A3 PCT/US1999/026989 US9926989W WO0028071A3 WO 2000028071 A3 WO2000028071 A3 WO 2000028071A3 US 9926989 W US9926989 W US 9926989W WO 0028071 A3 WO0028071 A3 WO 0028071A3
Authority
WO
WIPO (PCT)
Prior art keywords
homocysteine
cystathionine
determination
pyruvate
ammonia
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1999/026989
Other languages
French (fr)
Other versions
WO2000028071A9 (en
WO2000028071A2 (en
Inventor
Leo Seman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genzyme Corp
Original Assignee
Genzyme Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genzyme Corp filed Critical Genzyme Corp
Priority to DK99968042T priority Critical patent/DK1129356T3/en
Priority to AT99968042T priority patent/ATE232301T1/en
Priority to JP2000581237A priority patent/JP4426725B2/en
Priority to DE69905276T priority patent/DE69905276T2/en
Priority to CA002350607A priority patent/CA2350607C/en
Priority to EP99968042A priority patent/EP1129356B1/en
Priority to AU24737/00A priority patent/AU777349B2/en
Publication of WO2000028071A2 publication Critical patent/WO2000028071A2/en
Publication of WO2000028071A3 publication Critical patent/WO2000028071A3/en
Publication of WO2000028071A9 publication Critical patent/WO2000028071A9/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/527—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving lyase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6806—Determination of free amino acids
    • G01N33/6812—Assays for specific amino acids
    • G01N33/6815—Assays for specific amino acids containing sulfur, e.g. cysteine, cystine, methionine, homocysteine

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Microbiology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Adhesives Or Adhesive Processes (AREA)
  • Dental Preparations (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention relates to a method for determining homocysteine concentration in samples in which homocysteine is condensed using an enzyme cystathionine β synthase to form cystathionine. Pyruvate and/or ammonia are released from cystathionine by action of an enzyme, cystathionine β lyase, and homocysteine is regenerated. The release of pyruvate and/or ammonia can be correlated to the concentration of homocysteine present in the sample.
PCT/US1999/026989 1998-11-12 1999-11-12 A method for the determination of homocysteine Ceased WO2000028071A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
DK99968042T DK1129356T3 (en) 1998-11-12 1999-11-12 Method for determining homocysteine
AT99968042T ATE232301T1 (en) 1998-11-12 1999-11-12 METHOD FOR DETERMINING HOMOCYSTEIN
JP2000581237A JP4426725B2 (en) 1998-11-12 1999-11-12 Homocysteine measurement method
DE69905276T DE69905276T2 (en) 1998-11-12 1999-11-12 METHOD FOR DETERMINING HOMOCYSTONE
CA002350607A CA2350607C (en) 1998-11-12 1999-11-12 A method for the determination of homocysteine
EP99968042A EP1129356B1 (en) 1998-11-12 1999-11-12 A method for the determination of homocysteine
AU24737/00A AU777349B2 (en) 1998-11-12 1999-11-12 A method for the determination of homocysteine

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10809998P 1998-11-12 1998-11-12
US60/108,099 1998-11-12

Publications (3)

Publication Number Publication Date
WO2000028071A2 WO2000028071A2 (en) 2000-05-18
WO2000028071A3 true WO2000028071A3 (en) 2000-07-13
WO2000028071A9 WO2000028071A9 (en) 2000-09-28

Family

ID=22320298

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/026989 Ceased WO2000028071A2 (en) 1998-11-12 1999-11-12 A method for the determination of homocysteine

Country Status (9)

Country Link
EP (1) EP1129356B1 (en)
JP (1) JP4426725B2 (en)
AT (1) ATE232301T1 (en)
AU (1) AU777349B2 (en)
CA (1) CA2350607C (en)
DE (1) DE69905276T2 (en)
DK (1) DK1129356T3 (en)
ES (1) ES2189530T3 (en)
WO (1) WO2000028071A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6664073B1 (en) * 1999-11-02 2003-12-16 Catch Inc. Enzymatic cycling assays for homocysteine and cystathionine
US6635438B2 (en) 1999-11-02 2003-10-21 Catch, Inc. Enzymatic cycling assays for homocysteine and cystathionine
US6867012B2 (en) * 2000-12-05 2005-03-15 Toyo Boseki Kabushiki Kaisha Determination method of biological component and reagent kit used therefor
JP6056138B2 (en) * 2010-12-28 2017-01-11 東ソー株式会社 Immunological measurement method
CN111304283A (en) * 2020-03-05 2020-06-19 安徽大千生物工程有限公司 Kit for determining HCY based on cyclic enzyme rate method and preparation and use methods thereof
CN111983057A (en) * 2020-07-29 2020-11-24 上海润达榕嘉生物科技有限公司 A kind of detection method and kit for homocysteine in human plasma
CN116200457A (en) * 2023-01-17 2023-06-02 光景生物科技(苏州)有限公司 Homocysteine detection test strip and preparation method and application thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998007872A1 (en) * 1996-08-23 1998-02-26 The University Court Of The University Of Glasgow Homocysteine desulphurase from the protozoan trichomonas vaginalis
WO1999005311A1 (en) * 1997-07-24 1999-02-04 Anticancer, Inc. High specificity homocysteine assays for biological samples

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998007872A1 (en) * 1996-08-23 1998-02-26 The University Court Of The University Of Glasgow Homocysteine desulphurase from the protozoan trichomonas vaginalis
WO1999005311A1 (en) * 1997-07-24 1999-02-04 Anticancer, Inc. High specificity homocysteine assays for biological samples

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
F FRANTZEN, A L FAAREN, I ALFHEIM, A K NORDHEI: "Enzyme conversion immunoassay for determining total homocysteine in plasma or serum", CLINICAL CHEMISTRY, vol. 44, no. 2, February 1998 (1998-02-01), pages 311 - 316, XP002135758 *
P M UELAND, H REFSUM, S P STABLER, M R MALINOW, A ANDERSSON, R H ALLEN: "Total Homocysteine in Plasma or Serum: Methods and Clinical Applications", CLINICAL CHEMISTRY, vol. 39, no. 9, 1993, pages 1764 - 1779, XP002135759 *
T FISKERSTRAND, H REFSUM, G KVALHEIM, P M UELAND: "Homocysteine and Other Thiols in Plasma and Urine: Automated Determination and Sample Stability", CLINICAL CHEMISTRY, vol. 39, no. 2, 1993, pages 263 - 271, XP002135757 *

Also Published As

Publication number Publication date
WO2000028071A9 (en) 2000-09-28
ATE232301T1 (en) 2003-02-15
EP1129356B1 (en) 2003-02-05
AU2473700A (en) 2000-05-29
AU777349B2 (en) 2004-10-14
DE69905276D1 (en) 2003-03-13
DK1129356T3 (en) 2003-05-26
ES2189530T3 (en) 2003-07-01
JP2002529102A (en) 2002-09-10
EP1129356A2 (en) 2001-09-05
WO2000028071A2 (en) 2000-05-18
JP4426725B2 (en) 2010-03-03
DE69905276T2 (en) 2003-12-11
CA2350607C (en) 2009-04-07
CA2350607A1 (en) 2000-05-18

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