WO2000037663A3 - Plant transformation process - Google Patents

Plant transformation process Download PDF

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Publication number
WO2000037663A3
WO2000037663A3 PCT/US1999/030972 US9930972W WO0037663A3 WO 2000037663 A3 WO2000037663 A3 WO 2000037663A3 US 9930972 W US9930972 W US 9930972W WO 0037663 A3 WO0037663 A3 WO 0037663A3
Authority
WO
WIPO (PCT)
Prior art keywords
agrobacterium
seedlings
transgene
plant transformation
transformation process
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1999/030972
Other languages
French (fr)
Other versions
WO2000037663A2 (en
Inventor
Maria J Harrison
Anthony T Trieu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roberts Samuels Noble Foundation Inc
Original Assignee
Roberts Samuels Noble Foundation Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roberts Samuels Noble Foundation Inc filed Critical Roberts Samuels Noble Foundation Inc
Priority to CA002352488A priority Critical patent/CA2352488A1/en
Priority to AU25943/00A priority patent/AU2594300A/en
Priority to JP2000589717A priority patent/JP2002533090A/en
Priority to EP99968550A priority patent/EP1141356A2/en
Publication of WO2000037663A2 publication Critical patent/WO2000037663A2/en
Publication of WO2000037663A3 publication Critical patent/WO2000037663A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A method of Agrobacterium-mediated genetic transformation using seedlings has been found which is applicable to dicots and monocots capable of being transformed by Agrobacterium. The transformation method utilizes vacuum-infiltration to introduce the Agrobacterium T-DNA carrying a gene of interest into the seedlings. Upon maturity, seeds collected from the infiltrated seedlings are germinated, and progeny carrying the transgene are selected. This transformation method produces progeny exhibiting stable inheritance of the transgene without the need for regeneration methods such as somatic embryogenesis or organogenesis.
PCT/US1999/030972 1998-12-23 1999-12-23 Plant transformation process Ceased WO2000037663A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002352488A CA2352488A1 (en) 1998-12-23 1999-12-23 Plant transformation process
AU25943/00A AU2594300A (en) 1998-12-23 1999-12-23 Plant transformation process
JP2000589717A JP2002533090A (en) 1998-12-23 1999-12-23 Plant transformation method
EP99968550A EP1141356A2 (en) 1998-12-23 1999-12-23 Plant transformation process

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US11371798P 1998-12-23 1998-12-23
US60/113,717 1998-12-23
US14537399P 1999-07-23 1999-07-23
US60/145,373 1999-07-23

Publications (2)

Publication Number Publication Date
WO2000037663A2 WO2000037663A2 (en) 2000-06-29
WO2000037663A3 true WO2000037663A3 (en) 2000-11-09

Family

ID=26811389

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/030972 Ceased WO2000037663A2 (en) 1998-12-23 1999-12-23 Plant transformation process

Country Status (5)

Country Link
EP (1) EP1141356A2 (en)
JP (1) JP2002533090A (en)
AU (1) AU2594300A (en)
CA (1) CA2352488A1 (en)
WO (1) WO2000037663A2 (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6907887B2 (en) 2001-06-08 2005-06-21 Vector Tobacco Ltd. Modifying nicotine and nitrosamine levels in tobacco
US6911541B2 (en) 2000-08-30 2005-06-28 North Carolina State University Promoter fragment that is recognized by the product of the tobacco Nic gene
US7189570B2 (en) 2000-11-07 2007-03-13 North Carolina State University Putrescine-n-methyltransferase promoter
US7304220B2 (en) 1997-06-12 2007-12-04 North Carolina State University Regulation of quinolate phosphoribosyl transferase expression

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2386126C (en) * 2000-08-03 2010-07-13 Japan Tobacco Inc. Method of improving gene transfer efficiency into plant cells
KR100807434B1 (en) * 2000-08-03 2008-02-25 니뽄 다바코 산교 가부시키가이샤 How to improve the efficiency of gene introduction into plant cells
CN1149918C (en) * 2001-02-26 2004-05-19 山西省农业生物技术研究中心 Method for transferring agrobacterium mediated plant germination seed gene
EP1521840B1 (en) * 2002-07-16 2010-04-21 National Institute of Agrobiological Sciences Transformation of plant cells using electroporation and depressurization
SG178918A1 (en) 2009-09-22 2012-04-27 Medicago Inc Method of preparing plant-derived vlps
CA2786001A1 (en) 2009-12-31 2011-07-07 Pioneer Hi-Bred International, Inc. Engineering plant resistance to diseases caused by pathogens
EP2575432B1 (en) 2010-06-02 2019-08-14 Evolva, Inc. Recombinant production of steviol glycosides
CN103119169B (en) * 2010-06-09 2018-11-20 拜尔作物科学公司 Methods and tools for modifying plant genomes in nucleotide sequences commonly used in plant genome engineering
ES2614527T3 (en) 2010-11-04 2017-05-31 Medicago Inc. Plant expression system
WO2012084962A1 (en) * 2010-12-22 2012-06-28 Philip Morris Products S.A. Method and system for the vacuum infiltration of plants
TWI620816B (en) 2011-03-23 2018-04-11 苜蓿股份有限公司 Plant derived protein recovery method
CN102212553A (en) * 2011-04-15 2011-10-12 山西省农业科学院生物技术研究中心 Ultrasonic-assisted agrobacterium-mediated plant germination seed gene transformation method
AU2012294452C1 (en) 2011-08-08 2017-11-30 Danstar Ferment Ag Recombinant production of steviol glycosides
WO2014122227A2 (en) 2013-02-06 2014-08-14 Evolva Sa Methods for improved production of rebaudioside d and rebaudioside m
BR112015019160A2 (en) 2013-02-11 2017-08-22 Dalgaard Mikkelsen Michael PRODUCTION OF STEVIOL GLYCOSIDES IN RECOMBINANT HOSTERS
AU2015234595B2 (en) 2014-03-27 2021-03-25 Medicago Inc. Modified CPMV enhancer elements
WO2016004536A1 (en) 2014-07-11 2016-01-14 Medicago Inc. Modifying protein production in plants
CN114410493A (en) 2014-08-11 2022-04-29 埃沃尔瓦公司 Production of steviol glycosides in recombinant hosts
KR20170052647A (en) 2014-09-09 2017-05-12 에볼바 에스아 Production of steviol glycosides in recombinant hosts
AU2016211124B2 (en) 2015-01-30 2022-04-14 Danstar Ferment Ag Production of steviol glycosides in recombinant hosts
EP3271457B1 (en) 2015-03-16 2021-01-20 DSM IP Assets B.V. Udp-glycosyltransferases
RU2728472C2 (en) 2015-07-02 2020-07-29 Медикаго Инк. Activator of jasmonic acid signaling pathway
EP3332018B1 (en) 2015-08-07 2022-07-27 Evolva SA Production of steviol glycosides in recombinant hosts
KR20180132696A (en) 2016-04-13 2018-12-12 에볼바 에스아 Production of steviol glycosides in recombinant hosts
US10815514B2 (en) 2016-05-16 2020-10-27 Evolva Sa Production of steviol glycosides in recombinant hosts
WO2018083338A1 (en) 2016-11-07 2018-05-11 Evolva Sa Production of steviol glycosides in recombinant hosts
EP4351320A1 (en) 2021-06-09 2024-04-17 ImmunityBio, Inc. Methods and systems for producing a protein of interest in a plant
CN114657206B (en) * 2022-03-17 2024-03-22 中国热带农业科学院热带作物品种资源研究所 Genetic transformation method of stylosanthes guianensis

Citations (7)

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Publication number Priority date Publication date Assignee Title
WO1989005859A1 (en) * 1987-12-21 1989-06-29 The Upjohn Company Agrobacterium mediated transformation of germinating plant seeds
WO1994002620A2 (en) * 1992-07-27 1994-02-03 Pioneer Hi-Bred International, Inc. An improved method of agrobacterium-mediated transformation of cultured soybean cells
WO1995028423A1 (en) * 1994-04-13 1995-10-26 The General Hospital Corporation Rps gene family, primers, probes, and detection methods
US5569597A (en) * 1985-05-13 1996-10-29 Ciba Geigy Corp. Methods of inserting viral DNA into plant material
WO1997048814A2 (en) * 1996-06-21 1997-12-24 Monsanto Company Methods for the production of stably-transformed, fertile wheat employing agrobacterium-mediated transformation and compositions derived therefrom
WO1998013503A1 (en) * 1996-09-23 1998-04-02 F.B. Investments Pty. Ltd. A plant and method of modification
WO2000022149A1 (en) * 1998-10-15 2000-04-20 Protein Research Trust Transformation process

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5569597A (en) * 1985-05-13 1996-10-29 Ciba Geigy Corp. Methods of inserting viral DNA into plant material
WO1989005859A1 (en) * 1987-12-21 1989-06-29 The Upjohn Company Agrobacterium mediated transformation of germinating plant seeds
WO1994002620A2 (en) * 1992-07-27 1994-02-03 Pioneer Hi-Bred International, Inc. An improved method of agrobacterium-mediated transformation of cultured soybean cells
WO1995028423A1 (en) * 1994-04-13 1995-10-26 The General Hospital Corporation Rps gene family, primers, probes, and detection methods
WO1997048814A2 (en) * 1996-06-21 1997-12-24 Monsanto Company Methods for the production of stably-transformed, fertile wheat employing agrobacterium-mediated transformation and compositions derived therefrom
WO1998013503A1 (en) * 1996-09-23 1998-04-02 F.B. Investments Pty. Ltd. A plant and method of modification
WO2000022149A1 (en) * 1998-10-15 2000-04-20 Protein Research Trust Transformation process

Non-Patent Citations (11)

* Cited by examiner, † Cited by third party
Title
BECHTOLD NICOLE ET AL: "In planta Agrobacterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants.", COMPTES RENDUS DE L'ACADEMIE DES SCIENCES SERIE III SCIENCES DE LA VIE 1993, vol. 316, no. 10, 1993, pages 1194 - 1199, XP000907460, ISSN: 0764-4469 *
BIOLOGIA PLANTARUM (PRAGUE) 1997-1998, vol. 40, no. 1, 1997, pages 33 - 41, ISSN: 0006-3134 *
CHEE P P ET AL: "TRANSFORMATION OF SOYBEAN GLYCINE-MAX BY INFECTING GERMINATING SEEDS WITH AGROBACTERIUM-TUMEFACIENS", PLANT PHYSIOLOGY (BETHESDA) 1989, vol. 91, no. 3, 1989, pages 1212 - 1218, XP002140336, ISSN: 0032-0889 *
DANHOF LINDA R ET AL: "Transformation of Arabidopsis by vacuum infiltration: A demonstration.", ANNUAL MEETING OF THE AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS;SAN ANTONIO, TEXAS, USA; JULY 27-31, 1996, vol. 111, no. 2 SUPPL., 1996, Plant Physiology (Rockville) 1996, pages 164, XP002140332, ISSN: 0032-0889 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1995, BECHTOLD N ET AL: "In planta Agrobacterium-mediated transformation of adult Arabidopsis thaliana plants by vacuum infiltration.", XP002140338, Database accession no. PREV199698573015 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1996, TRIEU ANTHONY T ET AL: "Rapid transformation of Medicago truncatula: Regeneration via shoot organogenesis.", XP002140339, Database accession no. PREV199799329039 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1997, RAKOUSKY S ET AL: "Transient beta-glucuronidase activity after infiltration of Arabidopsis thaliana by Agrobacterium tumefaciens.", XP002140337, Database accession no. PREV199800165996 *
GENE TRANSFER TO PLANTS. 1995 SPRINGER-VERLAG;SPRINGER-VERLAG NEW YORK, INC. HEIDELBERGER PLATZ 3, D-1000 BERLIN, GERMANY; 175 FIFTH AVENUE, NEW YORK, NEW YORK 10010, USA, 1995, pages 19 - 23, ISBN: 3-540-58406-4 *
PLANT CELL REPORTS 1996, vol. 16, no. 1-2, 1996, pages 6 - 11, ISSN: 0721-7714 *
TRIEU ANTHONY T ET AL: "Approaches to improve the efficiency of transformation and regeneration of Medicago truncatula.", ANNUAL MEETING OF THE AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS;CHARLOTTE, NORTH CAROLINA, USA; JULY 29-AUGUST 2, 1995, vol. 108, no. 2 SUPPL., 1995, Plant Physiology (Rockville) 1995, pages 57, XP002140335, ISSN: 0032-0889 *
WENCK ALLAN ET AL: "Frequent collinear long transfer of DNA inclusive of the whole binary vector during Agrobacterium-mediated transformation.", PLANT MOLECULAR BIOLOGY 1997, vol. 34, no. 6, 1997, pages 913 - 922, XP002140334, ISSN: 0167-4412 *

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7304220B2 (en) 1997-06-12 2007-12-04 North Carolina State University Regulation of quinolate phosphoribosyl transferase expression
US7408098B2 (en) 1997-06-12 2008-08-05 North Carolina State University Regulation of quinolate phosphoribosyl transferase expression
US7425670B2 (en) 1997-06-12 2008-09-16 North Carolina State University Methods and compositions for protein production in tobacco plants with reduced nicotine
US7605308B2 (en) 1997-06-12 2009-10-20 North Carolina State University Regulation of quinolate phosphoribosyl transferase expression
US6911541B2 (en) 2000-08-30 2005-06-28 North Carolina State University Promoter fragment that is recognized by the product of the tobacco Nic gene
US7192771B2 (en) 2000-08-30 2007-03-20 North Carolina State University Plant promoter sequence
US7189570B2 (en) 2000-11-07 2007-03-13 North Carolina State University Putrescine-n-methyltransferase promoter
US6907887B2 (en) 2001-06-08 2005-06-21 Vector Tobacco Ltd. Modifying nicotine and nitrosamine levels in tobacco

Also Published As

Publication number Publication date
EP1141356A2 (en) 2001-10-10
JP2002533090A (en) 2002-10-08
AU2594300A (en) 2000-07-12
WO2000037663A2 (en) 2000-06-29
CA2352488A1 (en) 2000-06-29

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