WO2000043792A3 - Methods and kits for sequencing polypeptides - Google Patents

Methods and kits for sequencing polypeptides Download PDF

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Publication number
WO2000043792A3
WO2000043792A3 PCT/US2000/000790 US0000790W WO0043792A3 WO 2000043792 A3 WO2000043792 A3 WO 2000043792A3 US 0000790 W US0000790 W US 0000790W WO 0043792 A3 WO0043792 A3 WO 0043792A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
kits
present disclosure
sequencing polypeptides
polypeptides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2000/000790
Other languages
French (fr)
Other versions
WO2000043792A2 (en
Inventor
Thomas Woods Keough
Robert Scott Youngquist
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Procter and Gamble Co
Original Assignee
Procter and Gamble Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to DE60013093T priority Critical patent/DE60013093T2/en
Priority to AU28487/00A priority patent/AU757356B2/en
Priority to AT00906904T priority patent/ATE274190T1/en
Priority to IL14412900A priority patent/IL144129A0/en
Priority to EP00906904A priority patent/EP1145012B1/en
Priority to DK00906904T priority patent/DK1145012T3/en
Priority to CA002359163A priority patent/CA2359163C/en
Priority to JP2000595162A priority patent/JP2002535659A/en
Application filed by Procter and Gamble Co filed Critical Procter and Gamble Co
Publication of WO2000043792A2 publication Critical patent/WO2000043792A2/en
Publication of WO2000043792A3 publication Critical patent/WO2000043792A3/en
Priority to IL144129A priority patent/IL144129A/en
Priority to NO20013563A priority patent/NO20013563L/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
    • C07K1/128—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides
    • G01N33/6824—Sequencing of polypeptides involving N-terminal degradation, e.g. Edman degradation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G01N33/6851—Methods of protein analysis involving laser desorption ionisation mass spectrometry

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Genetics & Genomics (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present disclosure provides methods and kits which are useful for sequencing polypeptides. The methods involve derivatization of the N-terminus of the polypeptide or peptides thereof. Mass spectral analysis of one or more of the resulting derivatized analytes provides spectra which are readily interpreted through the use of techniques well-known to the ordinarily skilled artisan. The present disclosure also describes kits which enhance convenient performance of the methods.
PCT/US2000/000790 1999-01-20 2000-01-12 Methods and kits for sequencing polypeptides Ceased WO2000043792A2 (en)

Priority Applications (10)

Application Number Priority Date Filing Date Title
CA002359163A CA2359163C (en) 1999-01-20 2000-01-12 Methods and kits for sequencing polypeptides
AT00906904T ATE274190T1 (en) 1999-01-20 2000-01-12 METHODS AND KITS FOR SEQUENCING POLYPEPTIDES
IL14412900A IL144129A0 (en) 1999-01-20 2000-01-12 Methods and kits for sequencing polypeptides
EP00906904A EP1145012B1 (en) 1999-01-20 2000-01-12 Methods and kits for sequencing polypeptides
DK00906904T DK1145012T3 (en) 1999-01-20 2000-01-12 Methods and kits for sequencing polypeptides
DE60013093T DE60013093T2 (en) 1999-01-20 2000-01-12 METHODS AND KITS FOR SEQUENCING POLYPEPTIDES
AU28487/00A AU757356B2 (en) 1999-01-20 2000-01-12 Methods and kits for sequencing polypeptides
JP2000595162A JP2002535659A (en) 1999-01-20 2000-01-12 Methods and kits for determining the sequence of a polypeptide
IL144129A IL144129A (en) 1999-01-20 2001-07-03 Methods and kits for sequencing polypeptides
NO20013563A NO20013563L (en) 1999-01-20 2001-07-18 Methods and assays for sequencing polypeptides

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US11650299P 1999-01-20 1999-01-20
US15667799P 1999-09-29 1999-09-29
US60/116,502 1999-09-29
US60/156,677 1999-09-29

Publications (2)

Publication Number Publication Date
WO2000043792A2 WO2000043792A2 (en) 2000-07-27
WO2000043792A3 true WO2000043792A3 (en) 2000-11-30

Family

ID=26814315

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/000790 Ceased WO2000043792A2 (en) 1999-01-20 2000-01-12 Methods and kits for sequencing polypeptides

Country Status (12)

Country Link
EP (1) EP1145012B1 (en)
JP (1) JP2002535659A (en)
CN (1) CN1149398C (en)
AT (1) ATE274190T1 (en)
AU (1) AU757356B2 (en)
CA (1) CA2359163C (en)
DE (1) DE60013093T2 (en)
DK (1) DK1145012T3 (en)
ES (1) ES2223463T3 (en)
IL (2) IL144129A0 (en)
NO (1) NO20013563L (en)
WO (1) WO2000043792A2 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001270941A1 (en) * 2000-06-09 2001-12-17 Mds Proteomics, Inc. Labeling of proteomic samples during proteolysis for quantitation and sample multiplexing
US20060141632A1 (en) * 2000-07-25 2006-06-29 The Procter & Gamble Co. New methods and kits for sequencing polypeptides
US7045296B2 (en) * 2001-05-08 2006-05-16 Applera Corporation Process for analyzing protein samples
JP2005518521A (en) * 2001-05-23 2005-06-23 アメルシャム・バイオサイエンシーズ・アクチボラグ Peptide analysis using solid supports
US7074570B2 (en) 2001-05-23 2006-07-11 Ge Healthcare Bio-Sciences Ab Peptide fragmentation
JP3534191B1 (en) 2002-12-26 2004-06-07 日本電気株式会社 Method for analyzing peptide C-terminal amino acid sequence using mass spectrometry
US7371514B2 (en) 2004-07-16 2008-05-13 Agilent Technologies, Inc. Serial derivatization of peptides for de novo sequencing using tandem mass spectrometry
JP4543929B2 (en) * 2005-01-04 2010-09-15 日本電気株式会社 Protein analysis method
GB0503411D0 (en) * 2005-02-18 2005-03-30 Shimadzu Res Lab Europe Ltd Mass spectrometry precursor ion selection
HRP20100044B1 (en) 2010-01-25 2016-11-18 Institut Ruđer Bošković Protein identification using mass spectrometry
CA2836114C (en) * 2011-05-18 2016-10-25 Rudjer Boskovic Institute Method of detection of amino acid sequence and/or identification of peptides and proteins, by use of a new derivatization reagent and synthesis of 5-formyl-benzene-1,3-disulphonicacid as derivatization reagent
CN103852513B (en) * 2012-11-29 2016-01-06 中国科学院计算技术研究所 A kind of peptide section de novo sequencing method and system based on HCD and ETD mass spectrogram
CN108440740B (en) * 2018-02-14 2020-05-05 苏州大学 Reversible self-repairing epoxy resin and preparation and recycling remodeling methods thereof

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
CHEN X ET AL: "Isotope Edited Product Ion Assignment by alpha-N Labeling of Peptides with [H3(50%)]2,4-Dinitrofluorobenzene", JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY,US,ELSEVIER SCIENCE INC., NEW YORK, NY, vol. 10, no. 5, May 1999 (1999-05-01), pages 448 - 452, XP004164485, ISSN: 1044-0305 *
COX K A ET AL: "Role of the Site of Protonation in the Low-Energy Decompositions of Gas-Phase Peptide Ions", JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY,US,ELSEVIER SCIENCE INC, vol. 7, no. 6, 1 June 1996 (1996-06-01), pages 522 - 531, XP004052038, ISSN: 1044-0305 *
DATABASE CHEMICAL ABSTRACTS American Chemical Society (via STN, Karlsruhe); BAUER, MARK D.; SUN, YIPING; KEOUGH, THOMAS ; LACEY, MARTIN P.: "Sequencing of sulfonic acid derivatized peptides by electrospray mass spectrometry", XP002141711 *
HUANG Z.-H., WU J., ROTH K.D.W., YANG Y., GAGE D.A., WATSON J.T.: "A picomole-scale method for charge derivatization of peptides for sequence analysis by mass spectrometry", ANALYTICAL CHEMISTRY, vol. 69, 1997, pages 137 - 144, XP002141708 *
HUANG ZHI-HENG; SHEN TUNLI; WU JIANG; GAGE DOUGLAS A; WATSON J THROCK: "Protein sequencing by matrix-assisted laser desorption ionization-postsource decay-mass spectrometry analysis of the N-Tris(2,4,6-trimethoxyphenyl)phosphine-acetylated tryptic digests", ANALYTICAL BIOCHEMISTRY, vol. 268, 15 March 1999 (1999-03-15), pages 305 - 317, XP002141710 *
KEOUGH, T.; YOUNGQUIST, R. S.; LACEY, M. P.: "A method for high-sensitivity peptide sequencing using postsource decay matrix-assisted laser desorption ionization mass spectrometry", PROC. NATL. ACAD. SCI. U. S. A., vol. 96, June 1999 (1999-06-01), pages 7131 - 7136, XP002141709 *
NAKANISHI T ET AL: "Electrospray Ionization--Tandem Mass Spectrometry Analysis of Peptides Derived by Enzymatic Digestion of Oxidized Globin Subunits: An Improved Method to Determine Amino Acid Substitution in the Hemoglobin 'Core'", JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY,US,ELSEVIER SCIENCE INC, vol. 7, no. 10, 1 October 1996 (1996-10-01), pages 1040 - 1049, XP004070144, ISSN: 1044-0305 *
RAPID COMMUN. MASS SPECTROM., vol. 14, no. 10, 2000, pages 924 - 929 *
ROTH K.D.W., HUANG Z.-H., SADAGOPAN N., WATSON J.T.: "Charge derivatization of peptides for analysis by mass spectrometry", MASS SPECTROM. REV., vol. 17, 1998, pages 255, XP000922702 *

Also Published As

Publication number Publication date
IL144129A0 (en) 2002-05-23
AU757356B2 (en) 2003-02-20
NO20013563L (en) 2001-09-18
CA2359163A1 (en) 2000-07-27
ES2223463T3 (en) 2005-03-01
CN1149398C (en) 2004-05-12
DE60013093T2 (en) 2005-09-01
ATE274190T1 (en) 2004-09-15
CN1340162A (en) 2002-03-13
CA2359163C (en) 2004-07-20
IL144129A (en) 2006-12-31
WO2000043792A2 (en) 2000-07-27
JP2002535659A (en) 2002-10-22
EP1145012A2 (en) 2001-10-17
DE60013093D1 (en) 2004-09-23
EP1145012B1 (en) 2004-08-18
NO20013563D0 (en) 2001-07-18
AU2848700A (en) 2000-08-07
DK1145012T3 (en) 2004-12-13

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