WO2000055307A3 - A method for increasing the processivity of a dna- or rna-dependent polymerase and compositions therefor - Google Patents

A method for increasing the processivity of a dna- or rna-dependent polymerase and compositions therefor Download PDF

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Publication number
WO2000055307A3
WO2000055307A3 PCT/CA2000/000261 CA0000261W WO0055307A3 WO 2000055307 A3 WO2000055307 A3 WO 2000055307A3 CA 0000261 W CA0000261 W CA 0000261W WO 0055307 A3 WO0055307 A3 WO 0055307A3
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WO
WIPO (PCT)
Prior art keywords
increasing
dna
processivity
rna
polymerase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/CA2000/000261
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French (fr)
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WO2000055307A2 (en
Inventor
Jerry Pelletier
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
McGill University
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McGill University
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Filing date
Publication date
Application filed by McGill University filed Critical McGill University
Priority to EP00908881A priority Critical patent/EP1165760A2/en
Priority to CA002365263A priority patent/CA2365263A1/en
Publication of WO2000055307A2 publication Critical patent/WO2000055307A2/en
Publication of WO2000055307A3 publication Critical patent/WO2000055307A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to genetic engineering, and especially to cDNA synthesis and cDNA cloning. More specifically, a method is presented for increasing the processivity of a DNA- or RNA-dependent RNA- or DNA-polymerase comprising an addition of a general nucleic acid binding protein. In particular, the present invention relates to methods for increasing the processivity of reverse transcriptase (RT) E. coli DNA polymerase and T7 DNA polymerase using a nucleic acid binding protein such as Ncp7, recA, SSB and T4gp32. The invention further relates to assays to identify and select agents capable of increasing the processivity of a DNA or RNA-dependent polymerase, such as MMTV RT, AMV RT, T7 DNA polymerase and E. coli DNA polymerase. In a particularly preferred embodiment, the invention relates to a method for increasing the generation of full-length cDNA clones using a nucleic acid binding protein such as Ncp7, recA, SSB and T4gp32.
PCT/CA2000/000261 1999-03-12 2000-03-10 A method for increasing the processivity of a dna- or rna-dependent polymerase and compositions therefor Ceased WO2000055307A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP00908881A EP1165760A2 (en) 1999-03-12 2000-03-10 A method for increasing the processivity of a dna- or rna-dependent polymerase and compositions therefor
CA002365263A CA2365263A1 (en) 1999-03-12 2000-03-10 A method for increasing the processivity of a dna- or rna-dependent polymerase and compositions therefor

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12401199P 1999-03-12 1999-03-12
US60/124,011 1999-03-12

Publications (2)

Publication Number Publication Date
WO2000055307A2 WO2000055307A2 (en) 2000-09-21
WO2000055307A3 true WO2000055307A3 (en) 2001-08-16

Family

ID=22412246

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2000/000261 Ceased WO2000055307A2 (en) 1999-03-12 2000-03-10 A method for increasing the processivity of a dna- or rna-dependent polymerase and compositions therefor

Country Status (4)

Country Link
US (1) US20020119467A1 (en)
EP (1) EP1165760A2 (en)
CA (1) CA2365263A1 (en)
WO (1) WO2000055307A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020110827A1 (en) * 2001-02-12 2002-08-15 Hunter Craig P. Quantitative mRNA amplification
JP2005110621A (en) * 2003-10-10 2005-04-28 Aisin Seiki Co Ltd Nucleic acid amplification method and nucleic acid amplification reagent kit
US20060228726A1 (en) 2005-01-06 2006-10-12 Martin Patrick K Polypeptides having nucleic acid binding activity and compositions and methods for nucleic acid amplification
WO2007050125A2 (en) * 2005-05-27 2007-05-03 William Marsh Rice University High processivity polymerases
US20070059713A1 (en) 2005-09-09 2007-03-15 Lee Jun E SSB-DNA polymerase fusion proteins
US7998672B2 (en) 2006-05-30 2011-08-16 The University Of Utah Research Foundation Simultaneous amplification and detection of ribonucleic acid be an optical method using surface plasmon resonance
JP5279339B2 (en) 2008-05-16 2013-09-04 タカラバイオ株式会社 Composition for reverse transcription reaction
CN119452096A (en) * 2022-06-16 2025-02-14 生物辐射实验室股份有限公司 Improving the efficiency and sensitivity of single-cell assays using homologous recombinases
WO2025137293A2 (en) 2023-12-22 2025-06-26 Roche Sequencing Solutions, Inc. Enhancement of nucleic acid polymerization by aromatic compounds

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4331589A (en) * 1978-11-22 1982-05-25 Abbott Laboratories Deoxyribonucleic acid synthesis using binding protein extracted from chick embryo fibroblasts
WO1991017267A1 (en) * 1990-05-07 1991-11-14 Sri International Process for nucleic acid hybridization and amplification
EP0524808A2 (en) * 1991-07-23 1993-01-27 F. Hoffmann-La Roche Ag In situ PCR preparations, their use, and corresponding thermal cycler equipment
EP0821059A2 (en) * 1996-07-25 1998-01-28 The Institute Of Physical & Chemical Research Method for reverse transcription
EP0869187A2 (en) * 1997-02-24 1998-10-07 Becton, Dickinson and Company Replication of nucleic acids using single-strand DNA binding proteins

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5266466A (en) * 1987-01-14 1993-11-30 President And Fellows Of Harvard College Method of using T7 DNA polymerase to label the 3' end of a DNA molecule
US5654151A (en) * 1990-06-11 1997-08-05 Nexstar Pharmaceuticals, Inc. High affinity HIV Nucleocapsid nucleic acid ligands
US5438131A (en) * 1992-09-16 1995-08-01 Bergstrom; Donald E. 3-nitropyrrole nucleoside
US6183997B1 (en) * 1997-03-21 2001-02-06 Stratagene Polymerase enhancing factor (PEF) extracts PEF protein complexes isolated PEF proteins and methods for purifying and identifying same
US6054274A (en) * 1997-11-12 2000-04-25 Hewlett-Packard Company Method of amplifying the signal of target nucleic acid sequence analyte
US6406891B1 (en) * 1998-09-28 2002-06-18 Board Of Regents, The University Of Texas System Dual RT procedure for cDNA synthesis

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4331589A (en) * 1978-11-22 1982-05-25 Abbott Laboratories Deoxyribonucleic acid synthesis using binding protein extracted from chick embryo fibroblasts
WO1991017267A1 (en) * 1990-05-07 1991-11-14 Sri International Process for nucleic acid hybridization and amplification
EP0524808A2 (en) * 1991-07-23 1993-01-27 F. Hoffmann-La Roche Ag In situ PCR preparations, their use, and corresponding thermal cycler equipment
EP0821059A2 (en) * 1996-07-25 1998-01-28 The Institute Of Physical & Chemical Research Method for reverse transcription
EP0869187A2 (en) * 1997-02-24 1998-10-07 Becton, Dickinson and Company Replication of nucleic acids using single-strand DNA binding proteins

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
JI, X. ET AL.: "Effect of human immunodeficiency virus type 1 (HIV-1) nucleocapsid protein on HIV-1 reverse transcriptase activity in vitro", BIOCHEMISTRY., vol. 35, 1996, AMERICAN CHEMICAL SOCIETY. EASTON, PA., US, pages 132 - 142, XP000946067, ISSN: 0006-2960 *
LENER, D. ET AL.: "Involvement of HIV-1 nucleocapsid protein in the recruitment of reverse transcriptase into nucleoprotein complexes formed in vitro", JOURNAL OF BIOLOGICAL CHEMISTRY., vol. 273, 1998, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD., US, pages 33781 - 33786, XP000946066, ISSN: 0021-9258 *
LI X ET AL: "Human immunodeficiency virus Type 1 nucleocapsid protein (NCp7) directs specific initiation of minus-strand DNA synthesis primed by human tRNA(Lys3) in vitro: studies of viral RNA molecules mutated in regions that flank the primer binding site", JOURNAL OF VIROLOGY,US,THE AMERICAN SOCIETY FOR MICROBIOLOGY, vol. 70, no. 8, 1 August 1996 (1996-08-01), pages 4996 - 5004, XP002088584, ISSN: 0022-538X *
OSHIMA R O: "SINGLE-STRANDED DNA BINDING PROTEIN FACILITATES AMPLICATION OF GENOMIC SEQUENCES BY PCR", BIOTECHNIQUES,EATON PUBLISHING, NATICK,US, vol. 13, no. 128, 1992, pages 188, XP000971377, ISSN: 0736-6205 *
RIGLER MARY N ET AL: "Differences in the mechanism of stimulation of T7 DNA polymerase by two binding modes of Escherichia coli single-stranded DNA-binding protein", JOURNAL OF BIOLOGICAL CHEMISTRY,AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD,US, vol. 270, no. 15, 1995, pages 8910 - 8919, XP002155011, ISSN: 0021-9258 *
RODRIQUEZ-RODRIQUEZ, L. ET AL.: "Influence of human immunodeficiency virus nucleocapsid protein on synthesis and strand transfer by the reverse transcriptase in vitro", JOURNAL OF BIOLOGICAL CHEMISTRY., vol. 270, 1995, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD., US, pages 15005 - 15011, XP000946053, ISSN: 0021-9258 *
SCHWARZ K ET AL: "IMPROVED YIELDS OF LONG PCR PRODUCTS USING GENE 32 PROTEIN", NUCLEIC ACIDS RESEARCH,OXFORD UNIVERSITY PRESS, SURREY,GB, vol. 18, no. 4, 1990, pages 1079, XP000971373, ISSN: 0305-1048 *

Also Published As

Publication number Publication date
US20020119467A1 (en) 2002-08-29
WO2000055307A2 (en) 2000-09-21
CA2365263A1 (en) 2000-09-21
EP1165760A2 (en) 2002-01-02

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