WO2000056900A3 - Promoter sequences derived from fusarium venenatum and uses thereof - Google Patents

Promoter sequences derived from fusarium venenatum and uses thereof Download PDF

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Publication number
WO2000056900A3
WO2000056900A3 PCT/US2000/007815 US0007815W WO0056900A3 WO 2000056900 A3 WO2000056900 A3 WO 2000056900A3 US 0007815 W US0007815 W US 0007815W WO 0056900 A3 WO0056900 A3 WO 0056900A3
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WO
WIPO (PCT)
Prior art keywords
nucleic acid
nucleotides
seq
promoter sequences
polypeptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2000/007815
Other languages
French (fr)
Other versions
WO2000056900A2 (en
Inventor
Randy M Berka
Michael W Rey
Kimberly Brown
Stephen H Brown
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes Inc
Original Assignee
Novo Nordisk Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk Biotech Inc filed Critical Novo Nordisk Biotech Inc
Priority to EP00919596A priority Critical patent/EP1194572A2/en
Priority to JP2000606759A priority patent/JP4620253B2/en
Priority to AU40257/00A priority patent/AU4025700A/en
Publication of WO2000056900A2 publication Critical patent/WO2000056900A2/en
Anticipated expiration legal-status Critical
Publication of WO2000056900A3 publication Critical patent/WO2000056900A3/en
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention relates to methods for producing a polypetide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a first nucleic acid sequence encoding the polypeptide operably linked to a second nucleic acid sequence comprising a promoter foreign to the nucleic acid sequence, wherein the promoter comprises a sequence selected from the group consisting of nucleotides 1 to 3949 of SEQ ID NO. 1, nucleotides 1 to 938 of SEQ ID NO. 2, and nucleotides 1 to 3060 of SEQ ID NO. 3, and a subsequence thereof; and mutant, hybrid, and tandem promoters thereof; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to the isolated promoter sequences and to constructs, vectors, and fungal host cells comprising the promoter sequences operably linked to nucleic acid sequences encoding polypetides.
PCT/US2000/007815 1999-03-22 2000-03-22 Promoter sequences derived from fusarium venenatum and uses thereof Ceased WO2000056900A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP00919596A EP1194572A2 (en) 1999-03-22 2000-03-22 Promoter sequences derived from fusarium venenatum and uses thereof
JP2000606759A JP4620253B2 (en) 1999-03-22 2000-03-22 Promoter for gene expression in fungal cells
AU40257/00A AU4025700A (en) 1999-03-22 2000-03-22 Promoters for expressing genes in a fungal cell

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US27444999A 1999-03-22 1999-03-22
US09/274,449 1999-03-22

Publications (2)

Publication Number Publication Date
WO2000056900A2 WO2000056900A2 (en) 2000-09-28
WO2000056900A3 true WO2000056900A3 (en) 2002-01-31

Family

ID=23048245

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/007815 Ceased WO2000056900A2 (en) 1999-03-22 2000-03-22 Promoter sequences derived from fusarium venenatum and uses thereof

Country Status (6)

Country Link
EP (2) EP1194572A2 (en)
JP (4) JP4620253B2 (en)
CN (5) CN100532561C (en)
AU (1) AU4025700A (en)
DK (1) DK2278016T3 (en)
WO (1) WO2000056900A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024100063A1 (en) 2022-11-08 2024-05-16 River Stone Biotech Aps Genetically modified benzylisoquinoline alkaloid-producing host cells with modified efflux transporter gene expression

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WO2000056900A2 (en) 2000-09-28
EP1194572A2 (en) 2002-04-10

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