WO2000063408A3 - Apparatus for, and method of, introducing a substance into a cell by electroporation - Google Patents

Apparatus for, and method of, introducing a substance into a cell by electroporation Download PDF

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Publication number
WO2000063408A3
WO2000063408A3 PCT/GB2000/001477 GB0001477W WO0063408A3 WO 2000063408 A3 WO2000063408 A3 WO 2000063408A3 GB 0001477 W GB0001477 W GB 0001477W WO 0063408 A3 WO0063408 A3 WO 0063408A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell
substance
introducing
electroporation
transfected
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2000/001477
Other languages
French (fr)
Other versions
WO2000063408A2 (en
Inventor
John Dodgson
Anthony Robert Corless
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
AstraZeneca AB
Original Assignee
AstraZeneca AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by AstraZeneca AB filed Critical AstraZeneca AB
Priority to US09/958,755 priority Critical patent/US6653136B1/en
Priority to JP2000612486A priority patent/JP2004500022A/en
Priority to DE60029209T priority patent/DE60029209T2/en
Priority to EP00920881A priority patent/EP1173599B1/en
Priority to AU41296/00A priority patent/AU4129600A/en
Publication of WO2000063408A2 publication Critical patent/WO2000063408A2/en
Publication of WO2000063408A3 publication Critical patent/WO2000063408A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Electromagnetism (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

The invention relates to an apparatus for and method of introducing a substance into an object, particularly into a cell or cellular material. In a preferred arrangement apparatus receives the cell and preferably locates it between first and second electrodes and applies a voltage pulse to cause a disruption in the cell wall. This causes the cell to become permeable. The substance may then be introduced, for example under a fluid pressure. Cells may then be inspected and sorted into transfected and non-transfected types. This may be achieved automatically, for example by using electrophoresis.
PCT/GB2000/001477 1999-04-16 2000-04-17 Apparatus for, and method of, introducing a substance into a cell by electroporation Ceased WO2000063408A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
US09/958,755 US6653136B1 (en) 1999-04-16 2000-04-17 Apparatus for, and method of, introducing a substance into an object
JP2000612486A JP2004500022A (en) 1999-04-16 2000-04-17 Apparatus and method for introducing a substance into an object
DE60029209T DE60029209T2 (en) 1999-04-16 2000-04-17 DEVICE AND METHOD FOR INTRODUCING SUBSTANCES INTO A CELL BY ELECTROPORATION
EP00920881A EP1173599B1 (en) 1999-04-16 2000-04-17 Apparatus for, and method of, introducing a substance into a cell by electroporation
AU41296/00A AU4129600A (en) 1999-04-16 2000-04-17 Apparatus for, and method of, introducing a substance into an object

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9908681.1 1999-04-16
GBGB9908681.1A GB9908681D0 (en) 1999-04-16 1999-04-16 Apparatus for, and method of, introducing a substance into an object

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US09/958,755 A-371-Of-International US6653136B1 (en) 1999-04-16 2000-04-17 Apparatus for, and method of, introducing a substance into an object
US10/705,438 Continuation US20040106189A1 (en) 1999-04-16 2003-11-10 Apparatus for, and method of, introducing a substance into an object

Publications (2)

Publication Number Publication Date
WO2000063408A2 WO2000063408A2 (en) 2000-10-26
WO2000063408A3 true WO2000063408A3 (en) 2001-01-25

Family

ID=10851623

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/GB2000/001477 Ceased WO2000063408A2 (en) 1999-04-16 2000-04-17 Apparatus for, and method of, introducing a substance into a cell by electroporation
PCT/GB2000/001472 Ceased WO2000063407A2 (en) 1999-04-16 2000-04-17 Apparatus for, and method of, introducing a substance into a cell by electroporation

Family Applications After (1)

Application Number Title Priority Date Filing Date
PCT/GB2000/001472 Ceased WO2000063407A2 (en) 1999-04-16 2000-04-17 Apparatus for, and method of, introducing a substance into a cell by electroporation

Country Status (9)

Country Link
US (2) US6653136B1 (en)
EP (1) EP1173599B1 (en)
JP (1) JP2004500022A (en)
AT (1) ATE332387T1 (en)
AU (2) AU4129600A (en)
DE (1) DE60029209T2 (en)
ES (1) ES2267518T3 (en)
GB (1) GB9908681D0 (en)
WO (2) WO2000063408A2 (en)

Families Citing this family (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020006664A1 (en) 1999-09-17 2002-01-17 Sabatini David M. Arrayed transfection method and uses related thereto
DE60031997D1 (en) 1999-09-17 2007-01-04 Whitehead Biomedical Inst UMKEHRTRANSFEKTIONSVERFAHREN
EP2169383A1 (en) * 2001-06-15 2010-03-31 Bayer Technology Services GmbH Body for flow through cuvettes and use thereof
AU2003234323A1 (en) * 2002-05-03 2003-11-17 The Regents Of The University Of California Fast electrical lysis of cells and rapid collection of the contents thereof using capillary electrophoresis
EP1521840B1 (en) * 2002-07-16 2010-04-21 National Institute of Agrobiological Sciences Transformation of plant cells using electroporation and depressurization
US20040115784A1 (en) * 2002-09-30 2004-06-17 Maxcyte, Inc. Apparatus and method for streaming electroporation
US7087444B2 (en) * 2002-12-16 2006-08-08 Palo Alto Research Center Incorporated Method for integration of microelectronic components with microfluidic devices
JP2004305076A (en) * 2003-04-04 2004-11-04 Canon Inc Modifier for object
JP2004344036A (en) * 2003-05-21 2004-12-09 Fujitsu Ltd Substance introduction device and substance introduction system
US7338796B1 (en) * 2003-08-13 2008-03-04 Sandia Corporation Vesicle-based method and apparatus for collecting, manipulating, and chemically processing trace macromolecular species
US20050118705A1 (en) * 2003-11-07 2005-06-02 Rabbitt Richard D. Electrical detectors for microanalysis
JP2005278480A (en) * 2004-03-29 2005-10-13 Fujitsu Ltd Substance introduction device and substance introduction chip
US6897069B1 (en) 2004-06-08 2005-05-24 Ambion, Inc. System and method for electroporating a sample
CN103060191B (en) 2004-06-12 2015-03-11 英杰生命新加坡私人有限公司 Electroporator system
US7592139B2 (en) 2004-09-24 2009-09-22 Sandia National Laboratories High temperature flow-through device for rapid solubilization and analysis
JP4894199B2 (en) * 2005-02-17 2012-03-14 富士通株式会社 Substance injection equipment
US7704743B2 (en) * 2005-03-30 2010-04-27 Georgia Tech Research Corporation Electrosonic cell manipulation device and method of use thereof
US20060281168A1 (en) * 2005-06-13 2006-12-14 Tosoh Corporation Cell fusion chamber, cell fusion device, and method for cell fusion using the same
JP4659553B2 (en) * 2005-08-05 2011-03-30 富士通株式会社 Automatic microinjection device and cell capture plate
US20070105206A1 (en) * 2005-10-19 2007-05-10 Chang Lu Fluidic device
US8226811B2 (en) * 2005-11-02 2012-07-24 May-Ruben Technologies, Inc. High impedance system for generating electric fields and method of use
EP1957642A2 (en) * 2005-12-07 2008-08-20 Genetronics, Inc. Variable volume electroporation chamber and methods therefore
JP5040191B2 (en) * 2006-06-29 2012-10-03 富士通株式会社 Microinjection apparatus and automatic focus adjustment method
US20080138876A1 (en) * 2006-12-11 2008-06-12 Bio-Rad Laboratories, Inc. Transfection in electronically driven continuous flow
US9110010B2 (en) * 2007-05-11 2015-08-18 The United States Of America, As Represented By The Secretary Of The Navy Electrical detection using confined fluids
WO2009021232A2 (en) * 2007-08-09 2009-02-12 Massachusetts Institute Of Technology High-throughput, whole-animal screening system
US8308926B2 (en) 2007-08-20 2012-11-13 Purdue Research Foundation Microfluidic pumping based on dielectrophoresis
WO2009032827A2 (en) * 2007-09-04 2009-03-12 Purdue Research Foundation Electroporative flow cytometry
JP5226352B2 (en) * 2008-03-21 2013-07-03 オリンパス株式会社 Living body observation apparatus and living body observation method
KR101084528B1 (en) 2008-04-15 2011-11-18 인비트로겐 싱가포르 피티이. 엘티디. Pipette tip for electroporation device
US20090280518A1 (en) * 2008-05-12 2009-11-12 Massachusetts Institute Of Technology System for high throughput measurement of mechanical properties of cells
DE102010031103A1 (en) * 2010-07-08 2012-01-12 Robert Bosch Gmbh Method of manufacturing an integrated microfluidic system and integrated microfluidic system
WO2012147463A1 (en) * 2011-04-28 2012-11-01 株式会社日立製作所 Cell cultivation container and cell culturing apparatus
US20130101482A1 (en) * 2011-10-21 2013-04-25 Nanoinjection Technologies, L.L.C. Systems and devices for restraining a micro-object and associated methods
GB201312035D0 (en) 2013-07-04 2013-08-21 Cytomos Ltd Biological sensing apparatus
ES2751732T3 (en) 2014-05-02 2020-04-01 Lonza Cologne Gmbh Device and method for transfection of large volumes
EP3096338B1 (en) * 2015-05-21 2019-07-24 Nokia Technologies Oy An apparatus and method for providing a time varying voltage
EP3096134B1 (en) * 2015-05-21 2019-07-24 Nokia Technologies Oy An apparatus and method for providing a time varying voltage
ES2687422T3 (en) * 2015-09-07 2018-10-25 Miltenyi Biotec Gmbh Disposable electroporation cartridge
WO2017176764A1 (en) * 2016-04-04 2017-10-12 CyteQuest, Inc. System, device and method for electroporation of cells
US20170299571A1 (en) 2016-04-14 2017-10-19 Arizona Board Of Regents On Behalf Of The University Of Arizona Compositions and methods for determining mechanical properties of cells
WO2017210334A1 (en) * 2016-05-31 2017-12-07 Massachusetts Institute Of Technology Hydrodynamically controlled electric fields for high throughput transformation & high throughput parallel transformation platform
US11198841B2 (en) 2017-06-09 2021-12-14 Hewlett-Packard Development Company, L.P. Porated cell ejection devices
US11215562B2 (en) 2017-08-28 2022-01-04 Hewlett-Packard Development Company, L.P. Deformable covers on sensors and reservoirs
KR102232757B1 (en) * 2018-11-22 2021-03-26 (주)엑솔런스바이오테크놀로지 Delivery device of target material using extracorporeal shock wave
EP3666882A1 (en) 2018-12-12 2020-06-17 AIT Austrian Institute of Technology GmbH Specific electroporation and lysis of eukaryotic cells
EP3666881A1 (en) * 2018-12-12 2020-06-17 AIT Austrian Institute of Technology GmbH Coupled sorting and electric treatment of biological cells
KR102698373B1 (en) * 2019-05-03 2024-08-23 주식회사 엘지화학 Device and method for processing cell
KR102698372B1 (en) * 2019-05-03 2024-08-23 주식회사 엘지화학 Device and method for processing cell
WO2021010341A1 (en) 2019-07-12 2021-01-21 富士フイルム株式会社 Production method of organism-derived substance, production method of product, and voltage application device
EP4100506A4 (en) * 2020-04-07 2023-05-03 Hewlett-Packard Development Company, L.P. Microfluidic chip cell sorting and transfection
US20260015568A1 (en) * 2022-07-15 2026-01-15 Hewlett-Packard Development Company, L.P. Cell porating and optically detecting microfluidic devices
WO2026015154A1 (en) * 2024-07-12 2026-01-15 Hewlett-Packard Development Company, L.P. Microfluidic device with sensor

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4894343A (en) * 1986-11-19 1990-01-16 Hitachi, Ltd. Chamber plate for use in cell fusion and a process for production thereof
US5098843A (en) * 1987-06-04 1992-03-24 Calvin Noel M Apparatus for the high efficiency transformation of living cells
US5137817A (en) * 1990-10-05 1992-08-11 Amoco Corporation Apparatus and method for electroporation
US5283194A (en) * 1991-07-22 1994-02-01 Schmukler Robert E Apparatus and methods for electroporation and electrofusion
WO1998012310A1 (en) * 1996-09-23 1998-03-26 Duke University Method of transfecting cells by electroporation and apparatus for same
WO2000034436A2 (en) * 1998-12-04 2000-06-15 Genetronics, Inc. Facs assisted methods for introducing individual chromosomes into cells
WO2000037628A1 (en) * 1998-12-22 2000-06-29 Evotec Biosystems Ag Microsystem for cell permeation and cell fusion

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4822470A (en) * 1987-10-09 1989-04-18 Baylor College Of Medicine Method of and apparatus for cell poration and cell fusion using radiofrequency electrical pulses
US6090617A (en) * 1996-12-05 2000-07-18 Entremed, Inc. Flow electroporation chamber with electrodes having a crystalline metal nitride coating
WO1999011771A1 (en) * 1997-09-04 1999-03-11 Science Research Laboratory, Inc. Cell separation using electric fields
US6193647B1 (en) * 1999-04-08 2001-02-27 The Board Of Trustees Of The University Of Illinois Microfluidic embryo and/or oocyte handling device and method

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4894343A (en) * 1986-11-19 1990-01-16 Hitachi, Ltd. Chamber plate for use in cell fusion and a process for production thereof
US5098843A (en) * 1987-06-04 1992-03-24 Calvin Noel M Apparatus for the high efficiency transformation of living cells
US5137817A (en) * 1990-10-05 1992-08-11 Amoco Corporation Apparatus and method for electroporation
US5283194A (en) * 1991-07-22 1994-02-01 Schmukler Robert E Apparatus and methods for electroporation and electrofusion
WO1998012310A1 (en) * 1996-09-23 1998-03-26 Duke University Method of transfecting cells by electroporation and apparatus for same
WO2000034436A2 (en) * 1998-12-04 2000-06-15 Genetronics, Inc. Facs assisted methods for introducing individual chromosomes into cells
WO2000037628A1 (en) * 1998-12-22 2000-06-29 Evotec Biosystems Ag Microsystem for cell permeation and cell fusion

Also Published As

Publication number Publication date
ES2267518T3 (en) 2007-03-16
US20040106189A1 (en) 2004-06-03
WO2000063408A2 (en) 2000-10-26
US6653136B1 (en) 2003-11-25
EP1173599A2 (en) 2002-01-23
WO2000063407A2 (en) 2000-10-26
DE60029209T2 (en) 2007-06-06
GB9908681D0 (en) 1999-06-09
WO2000063407A3 (en) 2001-01-25
DE60029209D1 (en) 2006-08-17
ATE332387T1 (en) 2006-07-15
AU4129600A (en) 2000-11-02
JP2004500022A (en) 2004-01-08
EP1173599B1 (en) 2006-07-05
AU4582100A (en) 2000-11-02

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