WO2001016294A3 - Metal binding compounds and their use in cell culture medium compositions - Google Patents

Metal binding compounds and their use in cell culture medium compositions Download PDF

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Publication number
WO2001016294A3
WO2001016294A3 PCT/US2000/023580 US0023580W WO0116294A3 WO 2001016294 A3 WO2001016294 A3 WO 2001016294A3 US 0023580 W US0023580 W US 0023580W WO 0116294 A3 WO0116294 A3 WO 0116294A3
Authority
WO
WIPO (PCT)
Prior art keywords
compositions
binding compounds
metal binding
cells
added
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2000/023580
Other languages
French (fr)
Other versions
WO2001016294A2 (en
Inventor
David A Epstein
Paul Battista
Dale Gruber
David Judd
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Invitrogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Invitrogen Corp filed Critical Invitrogen Corp
Priority to DE2000637819 priority Critical patent/DE60037819T2/en
Priority to CA2383460A priority patent/CA2383460C/en
Priority to JP2001520842A priority patent/JP4972261B2/en
Priority to EP00959504A priority patent/EP1210410B1/en
Priority to AU70815/00A priority patent/AU7081500A/en
Publication of WO2001016294A2 publication Critical patent/WO2001016294A2/en
Publication of WO2001016294A3 publication Critical patent/WO2001016294A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0043—Medium free of human- or animal-derived components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/005—Protein-free medium
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/34—Sugars

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention is directed generally to metal binding compounds which may be added to cell culture media to replace factors required for cultivation of the cells (e.g. transferrin) which are of animal or human origin. More specifically, the invention is directed to metal binding compounds or complexes thereof comprising one or more transition element cations (such as ferrous or ferric ions), which are added to cell and tissue culture medium compositions. The metal binding compounds may be added to the media alone or may be first complexed with a transition metal ion. The invention is also directed to methods of use of such compositions, including, for example, methods for the cultivation of eukaryotic cells, particularly animal cells, in vitro. The invention also relates to compositions comprising such culture media and one or more cells, and to kits comprising one or more of the above-described compositions. The compositions of the present invention obviate the need for naturally derived metal-binding proteins, such as transferrin and ceruloplasmin, which may contain blood-borne pathogens.
PCT/US2000/023580 1999-08-27 2000-08-28 Metal binding compounds and their use in cell culture medium compositions Ceased WO2001016294A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
DE2000637819 DE60037819T2 (en) 1999-08-27 2000-08-28 METAL BINDING COMPOUNDS AND THEIR USE IN COMPOSITIONS FOR CELL CULTURE MEDIA
CA2383460A CA2383460C (en) 1999-08-27 2000-08-28 Metal binding compounds and their use in cell culture medium compositions
JP2001520842A JP4972261B2 (en) 1999-08-27 2000-08-28 Metal binding compounds and their use in cell culture media compositions
EP00959504A EP1210410B1 (en) 1999-08-27 2000-08-28 Metal binding compounds and their use in cell culture medium compositions
AU70815/00A AU7081500A (en) 1999-08-27 2000-08-28 Metal binding compounds and their use in cell culture medium compositions

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15105599P 1999-08-27 1999-08-27
US60/151,055 1999-08-27

Publications (2)

Publication Number Publication Date
WO2001016294A2 WO2001016294A2 (en) 2001-03-08
WO2001016294A3 true WO2001016294A3 (en) 2001-09-07

Family

ID=22537150

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/023580 Ceased WO2001016294A2 (en) 1999-08-27 2000-08-28 Metal binding compounds and their use in cell culture medium compositions

Country Status (7)

Country Link
EP (1) EP1210410B1 (en)
JP (1) JP4972261B2 (en)
AT (1) ATE384120T1 (en)
AU (1) AU7081500A (en)
CA (1) CA2383460C (en)
DE (1) DE60037819T2 (en)
WO (1) WO2001016294A2 (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030096414A1 (en) 2001-03-27 2003-05-22 Invitrogen Corporation Culture medium for cell growth and transfection
FR2836924B1 (en) * 2002-03-08 2005-01-14 Vivalis AVIAN CELL LINES USEFUL FOR THE PRODUCTION OF INTEREST SUBSTANCES
JP4833991B2 (en) 2004-11-02 2011-12-07 アレス トレーディング ソシエテ アノニム Serum-free cell culture medium for mammalian cells
US8273553B2 (en) 2004-11-02 2012-09-25 Ares Trading S.A. Production of growth hormone in serum-free cell culture medium for mammalian cells
DK2368975T3 (en) 2004-12-23 2015-01-05 Medimmune Llc Non-tumorigenic MDCK cell line for the propagation of viruses
EP1870450A1 (en) * 2006-06-21 2007-12-26 DSMIP Assets B.V. Process for the culturing of E1-immortalized HER cells
KR101464783B1 (en) 2006-09-15 2014-11-26 메디뮨 엘엘씨 MDCK cell line supporting high reverse transverse viral growth and bioreactor process using same
DK2250251T3 (en) 2008-01-09 2018-01-22 Sartorius Stedim Cellca Gmbh Improved culture medium additive and method of using it
KR20110074563A (en) 2008-09-24 2011-06-30 메디뮨 엘엘씨 Purification method of virus
WO2010115634A1 (en) 2009-04-09 2010-10-14 Cellca Gmbh Method for improved single cell cloning
BR112012027434B1 (en) 2010-04-26 2020-09-29 Novartis Ag PROCESS FOR PRODUCTION OF A RECOMBINANT POLYPEPTIDE UNDERSTANDING A PRODUCTION PHASE
BR112014027378A2 (en) 2012-05-02 2017-06-27 Life Technologies Corp high-yield transient expression in mammalian cells using unique high density growth-matched transfection medium and expression enhancers
JP2017506511A (en) * 2014-02-14 2017-03-09 ナショナル ユニバーシティー オブ アイルランド， ゴールウェイ Serum-free medium
EP3322813A1 (en) 2015-07-13 2018-05-23 Life Technologies Corporation System and method for improved transient protein expression in cho cells
CN108291190A (en) * 2015-12-03 2018-07-17 默克专利股份公司 The culture medium that chemical composition for detecting microorganism determines
EP3778868B1 (en) * 2019-08-16 2022-01-26 UGA Biopharma GmbH Cell culture medium for cultivating cells, method for culturing cells and method for expressing at least one recombinant protein in a cell culture
WO2026036019A1 (en) 2024-08-09 2026-02-12 Life Technologies Corporation Method of producing a recombinant protein in hek-293 cells

Citations (6)

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Publication number Priority date Publication date Assignee Title
EP0274445A2 (en) * 1987-01-09 1988-07-13 Medi-Cult A/S A serum-free growth medium additive and a use thereof
WO1993000423A1 (en) * 1991-06-21 1993-01-07 Novo Nordisk A/S Iron chelate culture medium additive
US5419894A (en) * 1983-07-01 1995-05-30 Schering Aktiengesellschaft Complexing agents, complexes and complex salts
WO1997034999A1 (en) * 1996-03-18 1997-09-25 University Of Pittsburgh Cell culture media for mammalian cells
US5688815A (en) * 1995-09-29 1997-11-18 Ciba Geigy Corporation Hydroxypyridinones
WO1998015614A1 (en) * 1996-10-10 1998-04-16 Life Technologies, Inc. Animal cell culture media comprising plant-derived nutrients

Family Cites Families (8)

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DE4033419A1 (en) * 1990-10-20 1992-04-23 Wolman Gmbh Dr POLYMOUS NITROGEN COMPOUNDS AND METAL FIXING SAEURS CONTAINING WOOD PROTECTION AGENTS
CZ282939B6 (en) * 1992-03-04 1997-11-12 Lonza A.G. NITROGENOUS HYDROXY HETEROCYCLIC CARBOXYLIC ACIDS, PROCESS OF THEIR PREPARATION AND MICRO-ORGANISMS Ki101DSN6920
US5328913A (en) * 1992-12-11 1994-07-12 Duke University Minoxidil analogs as inhibitors of cell proliferation and lysyl hydroxylase
JP3502420B2 (en) * 1993-01-21 2004-03-02 日本製薬株式会社 Animal cell culture medium
JPH07123990A (en) * 1993-11-08 1995-05-16 Cosmo Sogo Kenkyusho:Kk Method for producing 2,5-dihydroxypyridine and synthase of 2,5-dihydroxypyridine
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Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5419894A (en) * 1983-07-01 1995-05-30 Schering Aktiengesellschaft Complexing agents, complexes and complex salts
EP0274445A2 (en) * 1987-01-09 1988-07-13 Medi-Cult A/S A serum-free growth medium additive and a use thereof
WO1993000423A1 (en) * 1991-06-21 1993-01-07 Novo Nordisk A/S Iron chelate culture medium additive
US5688815A (en) * 1995-09-29 1997-11-18 Ciba Geigy Corporation Hydroxypyridinones
WO1997034999A1 (en) * 1996-03-18 1997-09-25 University Of Pittsburgh Cell culture media for mammalian cells
WO1998015614A1 (en) * 1996-10-10 1998-04-16 Life Technologies, Inc. Animal cell culture media comprising plant-derived nutrients

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
KRIEGERBECKOVA KARIN ET AL: "Non-transferrin iron uptake by HeLa cells cultured in serum-free media with different iron sources.", EUROPEAN JOURNAL OF CLINICAL CHEMISTRY AND CLINICAL BIOCHEMISTRY, vol. 33, no. 11, 1995, pages 791 - 797, XP000990410, ISSN: 0939-4974 *

Also Published As

Publication number Publication date
DE60037819T2 (en) 2009-01-08
EP1210410A2 (en) 2002-06-05
EP1210410B1 (en) 2008-01-16
JP2003508046A (en) 2003-03-04
WO2001016294A2 (en) 2001-03-08
DE60037819D1 (en) 2008-03-06
CA2383460A1 (en) 2001-03-08
JP4972261B2 (en) 2012-07-11
ATE384120T1 (en) 2008-02-15
AU7081500A (en) 2001-03-26
CA2383460C (en) 2015-10-13

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