WO2001018058A3 - Enhanced phage display library of human vh fragments and methods for producing same - Google Patents

Enhanced phage display library of human vh fragments and methods for producing same Download PDF

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Publication number
WO2001018058A3
WO2001018058A3 PCT/CA2000/001027 CA0001027W WO0118058A3 WO 2001018058 A3 WO2001018058 A3 WO 2001018058A3 CA 0001027 W CA0001027 W CA 0001027W WO 0118058 A3 WO0118058 A3 WO 0118058A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
fragments
phage display
human
display library
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/CA2000/001027
Other languages
French (fr)
Other versions
WO2001018058A2 (en
Inventor
Howard Kaplan
Joycelyn Entwistle
Jamshid Tanha
Saran Narang
Michael Dan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Viventia Bio Inc
Original Assignee
Novopharm Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CA 2282179 external-priority patent/CA2282179A1/en
Application filed by Novopharm Biotech Inc filed Critical Novopharm Biotech Inc
Priority to AU72627/00A priority Critical patent/AU7262700A/en
Priority to CA002384388A priority patent/CA2384388A1/en
Priority to EP00960243A priority patent/EP1214352A2/en
Publication of WO2001018058A2 publication Critical patent/WO2001018058A2/en
Publication of WO2001018058A3 publication Critical patent/WO2001018058A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Phage display libraries are taught in which the recombinant phage population displays a plurality of potential binding fragments having preferred characteristics of solubility and/or intermolecular interaction. Also taught are methods of biasing display libraries to produce variants which more closely approximate the preferred characteristics of the parental binding fragment.
PCT/CA2000/001027 1999-09-07 2000-09-07 Enhanced phage display library of human vh fragments and methods for producing same Ceased WO2001018058A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU72627/00A AU7262700A (en) 1999-09-07 2000-09-07 Enhanced phage display library of human vh fragments and methods for producing same
CA002384388A CA2384388A1 (en) 1999-09-07 2000-09-07 Enhanced phage display library of human vh fragments and methods for producing same
EP00960243A EP1214352A2 (en) 1999-09-07 2000-09-07 Enhanced phage display libraries of human vh fragments and methods for producing same

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
CA 2282179 CA2282179A1 (en) 1999-09-07 1999-09-07 Enhanced phage display library
CA2,282,179 1999-09-07
US16354699P 1999-11-04 1999-11-04
US60/163,546 1999-11-04

Publications (2)

Publication Number Publication Date
WO2001018058A2 WO2001018058A2 (en) 2001-03-15
WO2001018058A3 true WO2001018058A3 (en) 2001-10-04

Family

ID=25681199

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2000/001027 Ceased WO2001018058A2 (en) 1999-09-07 2000-09-07 Enhanced phage display library of human vh fragments and methods for producing same

Country Status (3)

Country Link
EP (1) EP1214352A2 (en)
AU (1) AU7262700A (en)
WO (1) WO2001018058A2 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1306272C (en) 2000-11-17 2007-03-21 罗切斯特大学 Method of screening for encoding antigen-specific immunoglobulin molecules or antigen-specific fragments thereof
ES2376454T3 (en) * 2000-12-22 2012-03-14 Grad, Carole, Legal Representative Of Howard, Kaplan EXPRESSION BOOKS IN FAGO OF VH HUMAN FRAGMENTS.
AU2002359913A1 (en) * 2001-12-20 2003-07-09 Agricultural Research Council Semi-synthetic antibody fragment combinatorial library based on avian immunoglobulin genes
JP4889493B2 (en) * 2003-05-14 2012-03-07 ドマンティス リミテッド Method for recovering a polypeptide that unfolds reversibly from a polypeptide repertoire
CN100575489C (en) * 2003-05-14 2009-12-30 多曼蒂斯有限公司 Method for recovering reversibly unfolded polypeptides from polypeptide totipotent library
WO2006081473A2 (en) 2005-01-28 2006-08-03 Children's Medical Center Corporation Methods for diagnosis and prognosis of epithelial cancers
JP2009527735A (en) 2006-02-17 2009-07-30 チルドレンズ メディカル センター コーポレーション Free NGAL as a biomarker for cancer
EP2010917B1 (en) 2006-04-10 2012-06-27 Trustees of Boston University Melanoma biomarker and methods of uses
EP3127549B1 (en) 2007-06-22 2019-12-18 Children's Medical Center Corporation Methods and uses thereof of a fragment of saposin a
EP3913367A1 (en) 2008-12-30 2021-11-24 Children's Medical Center Corporation Method of predicting acute appendicitis
WO2011069093A1 (en) 2009-12-04 2011-06-09 Boston Biomedical Research Institute, Inc. Detecting and counting tissue-specific stem cells and uses thereof
EP3366695B1 (en) 2009-12-17 2021-07-07 Children's Medical Center Corporation Saposin-a derived peptides and uses thereof
EP3560509B1 (en) 2011-12-22 2024-01-31 Children's Medical Center Corporation Saposin-a derived peptides and uses thereof
CN103215255B (en) * 2012-01-19 2015-06-17 深圳华大基因科技有限公司 Primer set for amplifying immunoglobulin light chain CDR3 sequence and application thereof
CN113234142B (en) 2012-08-22 2024-03-05 财团法人牧岩生命工学研究所 Screening and transformation methods for ultra-stable immunoglobulin variable domains and their applications

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995035374A1 (en) * 1994-06-21 1995-12-28 Dan Michael D Human monoclonal antibodies specific to cell cycle independent glioma surface antigen
WO2000029004A1 (en) * 1998-11-18 2000-05-25 Peptor Ltd. Small functional units of antibody heavy chain variable regions

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995035374A1 (en) * 1994-06-21 1995-12-28 Dan Michael D Human monoclonal antibodies specific to cell cycle independent glioma surface antigen
WO2000029004A1 (en) * 1998-11-18 2000-05-25 Peptor Ltd. Small functional units of antibody heavy chain variable regions

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
A. DESMYTER ET AL.: "Crystal structure of a camel single-domain VH antibody fragment in complex with lysozyme.", NATURE STRUCTURAL BIOLOGY, vol. 3, no. 9, September 1996 (1996-09-01), New York, NY, USA, pages 803 - 811, XP000990754 *
J. DAVIES ET AL.: "Single antibody domains as small recognition units: design and in vitro antigen selection of camelized, human VH domains with improved protein stability.", PROTEIN ENGINEERING, vol. 9, no. 6, 1996, Oxford, GB, pages 531 - 537, XP000971767 *
J. DE KRUIF ET AL.: "Selection and application of human single chain Fv antibody fragments from a semi-synthetic phage antibody display library with designed CDR3 regions.", JOURNAL OF MOLECULAR BIOLOGY, vol. 248, no. 1, 1995, Oxford, GB, pages 97 - 105, XP000646544 *
M. LAUWEREYS ET AL.: "Potent enzyme inhibitors derived from dromedary heavy-chain antibodies.", THE EMBO JOURNAL, vol. 17, no. 13, 1 July 1998 (1998-07-01), Oxford, GB, pages 3512 - 3520, XP002136362 *
S. MUYLDERMANS ET AL.: "Sequence and structure of VH domain from naturally occurring camel heavy chain immunoglobulins lacking light chains.", PROTEIN ENGINEERING, vol. 7, no. 9, September 1994 (1994-09-01), Oxford, GB, pages 1129 - 1135, XP000445081 *
Y. REITER ET AL.: "An antibody single-domain phage display library of a native heavy chain variable region: Isolation of functional single-domain VH molecules with a unique interface.", JOURNAL OF MOLECULAR BIOLOGY, vol. 290, no. 3, 16 July 1999 (1999-07-16), Oxford, GB, pages 685 - 698, XP002165613 *

Also Published As

Publication number Publication date
AU7262700A (en) 2001-04-10
WO2001018058A2 (en) 2001-03-15
EP1214352A2 (en) 2002-06-19

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