WO2001027150A3 - Anthozoa derived chromo/fluoroproteins and methods for using the same - Google Patents

Anthozoa derived chromo/fluoroproteins and methods for using the same Download PDF

Info

Publication number
WO2001027150A3
WO2001027150A3 PCT/US2000/028477 US0028477W WO0127150A3 WO 2001027150 A3 WO2001027150 A3 WO 2001027150A3 US 0028477 W US0028477 W US 0028477W WO 0127150 A3 WO0127150 A3 WO 0127150A3
Authority
WO
WIPO (PCT)
Prior art keywords
discosoma
fluoroproteins
chromo
same
anthozoa
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2000/028477
Other languages
French (fr)
Other versions
WO2001027150A2 (en
Inventor
Sergey Anatolievich Lukyanov
Arcady Fedorovich Fradkov
Yulii Aleksandrovich Labas
Mikhail Vladimirovich Matz
Alexey Terskikh
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Takara Bio USA Inc
Original Assignee
Clontech Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US1999/029405 external-priority patent/WO2000034526A1/en
Priority to NZ517548A priority Critical patent/NZ517548A/en
Priority to JP2001530368A priority patent/JP2003527833A/en
Priority to AU10867/01A priority patent/AU783767B2/en
Priority to CA2383642A priority patent/CA2383642C/en
Priority to EP00972169A priority patent/EP1305412A2/en
Application filed by Clontech Laboratories Inc filed Critical Clontech Laboratories Inc
Publication of WO2001027150A2 publication Critical patent/WO2001027150A2/en
Priority to US10/006,922 priority patent/US7166444B2/en
Publication of WO2001027150A3 publication Critical patent/WO2001027150A3/en
Anticipated expiration legal-status Critical
Priority to US11/607,319 priority patent/US7338782B2/en
Priority to US11/607,828 priority patent/US9834584B2/en
Priority to US11/607,818 priority patent/US8012682B2/en
Priority to US11/607,290 priority patent/US7442521B2/en
Priority to US11/607,422 priority patent/US7442522B2/en
Priority to US11/607,735 priority patent/US7338785B2/en
Priority to US11/607,666 priority patent/US7344862B2/en
Priority to US11/607,313 priority patent/US7537915B2/en
Priority to US11/607,706 priority patent/US7338784B2/en
Priority to US11/607,685 priority patent/US7338783B2/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

Anthozoa derived chromo/fluoroproteins and mutants thereof, as well as nucleic acid compositions encoding the same, are provided. Specific proteins of interest include chromo/fluoroproteins from the following specific anthozoa species: Anemonia majano, Clavularia sp., Zoanthus sp., Zoanthus sp., Discosoma striata, Discosoma sp. 'red', Anemonia sulcata, Discosoma sp. 'green', Discosoma sp. 'magenta', and mutants thereof. Also provided are fragments of the subject proteins and nucleic acids encoding the same, as well as antibodies to the subject proteins and transgenic cells and organisms. The subject protein and nucleic acid compositions find use in a variety of different applications. Finally, kits for use in such applications that include the subject proteins are provided.
PCT/US2000/028477 1998-12-11 2000-10-13 Anthozoa derived chromo/fluoroproteins and methods for using the same Ceased WO2001027150A2 (en)

Priority Applications (16)

Application Number Priority Date Filing Date Title
NZ517548A NZ517548A (en) 1999-10-14 2000-10-13 Non-bioluminescent Anthozoa derived chromophores / fluorophores and methods for using the same
JP2001530368A JP2003527833A (en) 1999-10-14 2000-10-13 Chromophores / phosphors derived from flower insects and their use
AU10867/01A AU783767B2 (en) 1999-10-14 2000-10-13 Anthozoa derived chromophores/fluorophores and methods for using the same
CA2383642A CA2383642C (en) 1999-10-14 2000-10-13 Anthozoa derived chromophores/fluorophores and methods for using the same
EP00972169A EP1305412A2 (en) 1999-10-14 2000-10-13 Anthozoa derived chromo/fluoroproteins and methods for using the same
US10/006,922 US7166444B2 (en) 1999-10-14 2001-12-04 Nucleic acids encoding chromophores/fluorophores and methods for using the same
US11/607,685 US7338783B2 (en) 1998-12-11 2006-11-30 Nucleic acids encoding chromophores/fluorophores and methods for using the same
US11/607,706 US7338784B2 (en) 1998-12-11 2006-11-30 Nucleic acids encoding chromophores/fluorophores and methods for using the same
US11/607,313 US7537915B2 (en) 1998-12-11 2006-11-30 Nucleic acids encoding chromophores/fluorophores and methods for using the same
US11/607,319 US7338782B2 (en) 1998-12-11 2006-11-30 Nucleic acids encoding chromophores/fluorophores and methods for using the same
US11/607,666 US7344862B2 (en) 1998-12-11 2006-11-30 Nucleic acids encoding chromophores/fluorophores and methods for using the same
US11/607,828 US9834584B2 (en) 1998-12-11 2006-11-30 Nucleic acids encoding chromophores/fluorophores and methods for using the same
US11/607,818 US8012682B2 (en) 1998-12-11 2006-11-30 Nucleic acids encoding chromophores/fluorophores and methods for using the same
US11/607,290 US7442521B2 (en) 1998-12-11 2006-11-30 Nucleic acids encoding chromophores/fluorophores and methods for using the same
US11/607,422 US7442522B2 (en) 1998-12-11 2006-11-30 Nucleic acids encoding chromo or fluorescent proteins and method for using the same
US11/607,735 US7338785B2 (en) 1998-12-11 2006-11-30 Nucleic acids encoding chromophores/fluorophores and methods for using the same

Applications Claiming Priority (38)

Application Number Priority Date Filing Date Title
US41852999A 1999-10-14 1999-10-14
US09/418,529 1999-10-14
US41892299A 1999-10-15 1999-10-15
US41891799A 1999-10-15 1999-10-15
US09/418,917 1999-10-15
US09/418,922 1999-10-15
US44433899A 1999-11-19 1999-11-19
US44434199A 1999-11-19 1999-11-19
US09/444,338 1999-11-19
US09/444,341 1999-11-19
US45814499A 1999-12-09 1999-12-09
US45847799A 1999-12-09 1999-12-09
US45755699A 1999-12-09 1999-12-09
US45789899A 1999-12-09 1999-12-09
US09/458,144 1999-12-09
US09/458,477 1999-12-09
US09/457,556 1999-12-09
US09/457,898 1999-12-09
PCT/US1999/029405 WO2000034526A1 (en) 1998-12-11 1999-12-10 Fluorescent proteins from non-bioluminescent species of class anthozoa, genes encoding such proteins and uses thereof
USPCT/US99/29405 1999-12-10
US21160700P 2000-06-14 2000-06-14
US21162700P 2000-06-14 2000-06-14
US21160900P 2000-06-14 2000-06-14
US21176600P 2000-06-14 2000-06-14
US21207000P 2000-06-14 2000-06-14
US21188800P 2000-06-14 2000-06-14
US21188000P 2000-06-14 2000-06-14
US21168700P 2000-06-14 2000-06-14
US21162600P 2000-06-14 2000-06-14
US60/211,627 2000-06-14
US60/211,609 2000-06-14
US60/211,880 2000-06-14
US60/211,766 2000-06-14
US60/211,888 2000-06-14
US60/211,626 2000-06-14
US60/211,607 2000-06-14
US60/211,687 2000-06-14
US60/212,070 2000-06-14

Related Parent Applications (12)

Application Number Title Priority Date Filing Date
US21033098A Division 1998-12-11 1998-12-11
US21033098A Continuation-In-Part 1998-12-11 1998-12-11
US41852999A Continuation-In-Part 1998-12-11 1999-10-14
US41892299A Continuation-In-Part 1998-12-11 1999-10-15
US41891799A Continuation-In-Part 1998-12-11 1999-10-15
US44433899A Continuation-In-Part 1998-12-11 1999-11-19
US44434199A Continuation-In-Part 1998-12-11 1999-11-19
US45847799A Continuation-In-Part 1998-12-11 1999-12-09
US45755699A Continuation-In-Part 1998-12-11 1999-12-09
US45814499A Continuation-In-Part 1998-12-11 1999-12-09
US45789899A Continuation-In-Part 1998-12-11 1999-12-09
PCT/US1999/029405 Continuation-In-Part WO2000034526A1 (en) 1998-12-11 1999-12-10 Fluorescent proteins from non-bioluminescent species of class anthozoa, genes encoding such proteins and uses thereof

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US10/000,922 Continuation-In-Part US20030087492A1 (en) 1998-12-11 2001-11-02 Semiconductor device and method of manufacturing the same
US10/006,922 Continuation-In-Part US7166444B2 (en) 1998-12-11 2001-12-04 Nucleic acids encoding chromophores/fluorophores and methods for using the same

Publications (2)

Publication Number Publication Date
WO2001027150A2 WO2001027150A2 (en) 2001-04-19
WO2001027150A3 true WO2001027150A3 (en) 2001-12-06

Family

ID=27586307

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/028477 Ceased WO2001027150A2 (en) 1998-12-11 2000-10-13 Anthozoa derived chromo/fluoroproteins and methods for using the same

Country Status (6)

Country Link
US (4) US7166444B2 (en)
EP (2) EP1305412A2 (en)
JP (1) JP2003527833A (en)
AU (1) AU783767B2 (en)
CA (1) CA2383642C (en)
WO (1) WO2001027150A2 (en)

Families Citing this family (60)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030087492A1 (en) 2001-11-02 2003-05-08 Promos Technologies, Inc. Semiconductor device and method of manufacturing the same
US9834584B2 (en) 1998-12-11 2017-12-05 Takara Bio Usa, Inc. Nucleic acids encoding chromophores/fluorophores and methods for using the same
US7338782B2 (en) 1998-12-11 2008-03-04 Clontech Laboratories, Inc. Nucleic acids encoding chromophores/fluorophores and methods for using the same
CA2383642C (en) 1999-10-14 2014-03-25 Clontech Laboratories, Inc. Anthozoa derived chromophores/fluorophores and methods for using the same
HK1049185A1 (en) * 2000-02-23 2003-05-02 Invitrogen Corporation Modified fluorescent proteins
AU6842401A (en) * 2000-06-14 2001-12-24 Clontech Lab Inc Fluorescent timer proteins and methods for their use
US7879540B1 (en) 2000-08-24 2011-02-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
JP4330338B2 (en) 2000-10-12 2009-09-16 クローンテック ラボラトリーズ インク． Fluorescent protein greatly shifted to the red side
US6969597B2 (en) 2001-02-21 2005-11-29 Clontech Laboratories, Inc. Nucleic acids encoding non aggregating fluorescent proteins and methods for using the same
JP2004536571A (en) * 2001-02-21 2004-12-09 クローンテック ラボラトリーズ インク． Non-aggregating fluorescent protein and method of using same
WO2002096924A1 (en) * 2001-05-25 2002-12-05 Clontech Laboratories, Inc. Kindling fluorescent proteins and methods for their use
CA2461824A1 (en) * 2001-10-12 2003-04-17 Clontech Laboratories, Inc. Nucleic acids encoding linked chromo/fluorescent domains and methods for using the same
EP1444245A4 (en) * 2001-11-13 2005-11-16 Clontech Lab Inc NOVEL CHROMOPHORES / FLUOROPHORES AND METHODS OF USE
GB0129689D0 (en) * 2001-12-12 2002-01-30 Janssen Pharmaceutica Nv Isolation tool for viable c-kit expressing cells
AU2002357322A1 (en) 2001-12-19 2003-07-09 The University Of Chicago Rapidly maturing fluorescent proteins and methods for using the same
EP2371953A1 (en) 2001-12-20 2011-10-05 Riken Fluorescent protein
JP4381147B2 (en) 2002-02-25 2009-12-09 独立行政法人理化学研究所 Fluorescent protein
US7504491B2 (en) 2002-06-10 2009-03-17 Riken Pigment protein from Cnidopus japonicus
AU2003242182A1 (en) * 2002-06-10 2003-12-22 Medical & Biological Laboratories Co., Ltd Chromoprotein
US20040180378A1 (en) * 2002-07-19 2004-09-16 Eileen Tozer Fluorescent proteins, nucleic acids encoding them and methods for making and using them
DE60335726D1 (en) 2002-08-23 2011-02-24 Riken Wako CHROMOPROTEIN AND FLUORO PROTEINS
US7344886B2 (en) 2002-11-29 2008-03-18 Boehringer Ingelheim Pharma Gmbh & Co., Kg Neomycin-phosphotransferase-genes and methods for the selection of recombinant cells producing high levels of a desired gene product
US7384744B2 (en) 2002-11-29 2008-06-10 Boehringer Ingelheim Pharma Gmbh & Co., Kg Expression vector, methods for the production of heterologous gene products and for the selection of recombinant cells producing high levels of such products
US7413874B2 (en) 2002-12-09 2008-08-19 University Of Miami Nucleic acid encoding fluorescent proteins from aquatic species
RU2345137C2 (en) 2002-12-26 2009-01-27 Закрытое акционерное общество "ЕВРОГЕН" Fluorescing proteins from copepodas crustaceas and their application
US7258981B2 (en) * 2003-01-24 2007-08-21 Clontech Laboratories, Inc. Sensitive proteasome sensor constructs and methods for their design and use
WO2004078984A1 (en) * 2003-03-05 2004-09-16 Janssen Pharmaceutica N.V. Animal model for the fast identification of pharmaceutical active compounds in vivo
EP2098535B1 (en) 2003-06-16 2011-10-05 Riken Fluorescent protein and chromoprotein
DE10338531A1 (en) 2003-08-19 2005-04-07 Boehringer Ingelheim Pharma Gmbh & Co. Kg Method for recloning production cells
US8669109B2 (en) 2003-08-19 2014-03-11 Boehringer Ingelheim Pharma Gmbh & Co. Kg Methods of producing proteins in Chinese hamster ovary (CHO) cells
EP1732944B1 (en) 2004-04-07 2012-09-05 The University of Chicago Monomeric red fluorescent proteins
US7728118B2 (en) 2004-09-17 2010-06-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
US7790868B2 (en) 2005-05-20 2010-09-07 The Governors Of The University Of Alberta Teal fluorescent proteins (mTFP1)
US7935801B2 (en) 2005-05-20 2011-05-03 The Governors Of The University Of Alberta Teal fluorescent proteins
US8362250B2 (en) 2005-05-24 2013-01-29 Enzo Biochem, Inc. Fluorescent dyes and compounds, methods and kits useful for identifying specific organelles and regions in cells of interest
WO2007019382A1 (en) * 2005-08-03 2007-02-15 University Of Sydney Photoactivatable chromo/fluorescent gfp-like proteins and imaging applications
EP3796002B1 (en) 2006-07-14 2023-11-22 The Regents of The University of California Cancer biomarkers and methods of use thereof
US20080124760A1 (en) 2006-07-26 2008-05-29 Barbara Enenkel Regulatory Nucleic Acid Elements
WO2008085502A2 (en) * 2007-01-05 2008-07-17 Inseron, Inc. A green fluorescent protein optimized for expression with self-cleaving polypeptides
WO2008150496A2 (en) * 2007-05-31 2008-12-11 Genelux Corporation Assay for sensitivity to chemotherapeutic agents
US8727554B2 (en) 2007-06-25 2014-05-20 Yorktown Technologies, L.P. Aquarium with adjustable lighting
WO2009059305A2 (en) 2007-11-01 2009-05-07 The University Of Chicago Red fluorescent proteins with enhanced bacterial expression, increased brightness and reduced aggregation
US9334281B2 (en) * 2008-09-08 2016-05-10 Enzo Life Sciences, Inc. Fluorochromes for organelle tracing and multi-color imaging
US9250249B2 (en) 2008-09-08 2016-02-02 Enzo Biochem, Inc. Autophagy and phospholipidosis pathway assays
JP5304275B2 (en) * 2009-01-29 2013-10-02 Ｊｎｃ株式会社 Codon optimized nucleic acid encoding apocrytin-II and methods of use thereof
US9145447B2 (en) * 2009-12-04 2015-09-29 Allele Biotechnology & Pharmaceuticals, Inc. Photoconvertible fluorescent proteins
WO2011133874A1 (en) * 2010-04-23 2011-10-27 University Of Massachusetts Multicistronic expression constructs
WO2011139721A1 (en) 2010-04-27 2011-11-10 The Regents Of The University Of California Cancer biomarkers and methods of use thereof
US10842728B2 (en) * 2010-08-09 2020-11-24 Allele Biotechnology & Pharmaceuticals, Inc. Light-absorbing compositions and methods of use
US9450759B2 (en) 2011-04-05 2016-09-20 Apple Inc. Apparatus and methods for controlling distribution of electronic access clients
WO2012155071A1 (en) 2011-05-12 2012-11-15 Gilead Biologics, Inc. Methods of identifying lysyl oxidase - like - 2 (loxl2) binding partners
WO2013138522A2 (en) 2012-03-16 2013-09-19 Genelux Corporation Methods for assessing effectiveness and monitoring oncolytic virus treatment
WO2013158265A1 (en) 2012-04-20 2013-10-24 Genelux Corporation Imaging methods for oncolytic virus therapy
EP2859331A2 (en) 2012-06-06 2015-04-15 The United States of America, as represented by The Secretary, Department of Health and Human Services Kits for detecting and monitoring endocrine disrupting chemicals (edcs)
US10221221B2 (en) 2012-07-24 2019-03-05 Allele Biotechnology & Pharmaceuticals, Inc Monomeric yellow-green fluorescent protein from cephalochordate
EP3037534B1 (en) 2013-08-23 2021-02-17 Riken Polypeptide exhibiting fluorescent properties, and utilization of same
WO2016090323A1 (en) 2014-12-05 2016-06-09 Prelude, Inc. Dcis recurrence and invasive breast cancer
EP3331536A4 (en) 2015-08-03 2019-03-27 The Regents of The University of California COMPOSITIONS AND METHODS FOR MODULATING ABHD2 ACTIVITY
US10386361B2 (en) 2016-06-07 2019-08-20 The Board Of Trustees Of The Leland Stanford Junior University Long term hematopoietic stem cell specific reporter mouse and uses thereof
WO2020056338A1 (en) 2018-09-14 2020-03-19 Prelude Corporation Method of selection for treatment of subjects at risk of invasive breast cancer

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19718640A1 (en) * 1997-05-02 1999-07-22 Joerg Wiedenmann Protein derived from Anemonia useful in gene technology and molecular biology
WO1999049019A2 (en) * 1998-03-27 1999-09-30 Prolume, Ltd. Luciferases, fluorescent proteins, nucleic acids encoding the luciferases and fluorescent proteins and the use thereof in diagnostics

Family Cites Families (95)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19718C (en) N. WlEDERER in Fürth (Bayern) Spring strut for mirrors and pictures
US802125A (en) 1903-12-15 1905-10-17 Barrett D Tillinghast Valve-gear for explosive-engines.
US796777A (en) 1905-01-31 1905-08-08 Luigi Viezzi Support for floor construction.
US828844A (en) 1905-12-12 1906-08-14 Reece Buttonhole Machine Company Button-sewing machine.
US972638A (en) 1909-12-03 1910-10-11 Frederick N Pease Metal-polish.
USRE30985E (en) 1978-01-01 1982-06-29 Serum-free cell culture media
US4302536A (en) 1978-08-15 1981-11-24 Longenecker Robert W Colorimetric immunoassay process
US4399216A (en) 1980-02-25 1983-08-16 The Trustees Of Columbia University Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
ZA811368B (en) 1980-03-24 1982-04-28 Genentech Inc Bacterial polypedtide expression employing tryptophan promoter-operator
US4551433A (en) 1981-05-18 1985-11-05 Genentech, Inc. Microbial hybrid promoters
US4560655A (en) 1982-12-16 1985-12-24 Immunex Corporation Serum-free cell culture medium and process for making same
US4657866A (en) 1982-12-21 1987-04-14 Sudhir Kumar Serum-free, synthetic, completely chemically defined tissue culture media
US4745051A (en) 1983-05-27 1988-05-17 The Texas A&M University System Method for producing a recombinant baculovirus expression vector
IE58011B1 (en) 1983-05-27 1993-06-16 Texas A & M Univ Sys Method for producing a recombinant baculovirus expression vector
US4767704A (en) 1983-10-07 1988-08-30 Columbia University In The City Of New York Protein-free culture medium
ZA848495B (en) 1984-01-31 1985-09-25 Idaho Res Found Production of polypeptides in insect cells
US4837148A (en) 1984-10-30 1989-06-06 Phillips Petroleum Company Autonomous replication sequences for yeast strains of the genus pichia
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
GB8516415D0 (en) 1985-06-28 1985-07-31 Celltech Ltd Culture of animal cells
US5583024A (en) 1985-12-02 1996-12-10 The Regents Of The University Of California Recombinant expression of Coleoptera luciferase
US4927762A (en) 1986-04-01 1990-05-22 Cell Enterprises, Inc. Cell culture medium with antioxidant
GB8610600D0 (en) 1986-04-30 1986-06-04 Novo Industri As Transformation of trichoderma
US5221623A (en) 1986-07-22 1993-06-22 Boyce Thompson Institute For Plant Research, Inc. Use of bacterial luciferase structural genes for cloning and monitoring gene expression in microorganisms and for tagging and identification of genetically engineered organisms
US5739409A (en) 1987-06-19 1998-04-14 The Regents Of The University Of California Endogenously sweetened transgenic plant products
US4929555A (en) 1987-10-19 1990-05-29 Phillips Petroleum Company Pichia transformation
US5182202A (en) 1987-11-30 1993-01-26 Kikkoman Corporation Purified luciferase from luciola cruciata
AU632065B2 (en) 1988-09-23 1992-12-17 Novartis Vaccines And Diagnostics, Inc. Cell culture medium for enhanced cell growth, culture longevity and product expression
GB8823869D0 (en) 1988-10-12 1988-11-16 Medical Res Council Production of antibodies
GB8904009D0 (en) 1989-02-22 1989-04-05 Celltech Ltd Vector
US5256558A (en) 1989-05-03 1993-10-26 The Trustees Of Rockefeller University Gene encoding plant asparagine synthetase
FR2649120B1 (en) 1989-06-30 1994-01-28 Cayla NOVEL STRAIN AND ITS MUTANTS OF FILAMENTOUS MUSHROOMS, PROCESS FOR PRODUCING RECOMBINANT PROTEINS USING SAID STRAIN, AND STRAINS AND PROTEINS OBTAINED BY SAID METHOD
US5292658A (en) 1989-12-29 1994-03-08 University Of Georgia Research Foundation, Inc. Boyd Graduate Studies Research Center Cloning and expressions of Renilla luciferase
US5484956A (en) 1990-01-22 1996-01-16 Dekalb Genetics Corporation Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin
US5219737A (en) 1990-03-27 1993-06-15 Kikkoman Corporation Mutant luciferase of a firefly, mutant luciferase genes, recombinant dnas containing the genes and a method of producing mutant luciferase
US5767367A (en) 1990-06-23 1998-06-16 Hoechst Aktiengesellschaft Zea mays (L.) with capability of long term, highly efficient plant regeneration including fertile transgenic maize plants having a heterologous gene, and their preparation
EP0537270B1 (en) 1990-07-02 1998-09-09 The Regents Of The University Of California Detection of analytes using fluorescent energy transfer
US5165424A (en) 1990-08-09 1992-11-24 Silverman Harvey N Method and system for whitening teeth
US5229285A (en) 1991-06-27 1993-07-20 Kikkoman Corporation Thermostable luciferase of firefly, thermostable luciferase gene of firefly, novel recombinant dna, and process for the preparation of thermostable luciferase of firefly
US5641670A (en) 1991-11-05 1997-06-24 Transkaryotic Therapies, Inc. Protein production and protein delivery
US5733761A (en) 1991-11-05 1998-03-31 Transkaryotic Therapies, Inc. Protein production and protein delivery
DE4207358A1 (en) 1992-03-04 1993-09-09 Inst Genbiologische Forschung EXPRESSION CASSETTE AND PLASMIDE FOR CLOSE-CELL-SPECIFIC EXPRESSION AND THEIR USE FOR THE PRODUCTION OF TRANSGENIC PLANT CELLS AND PLANTS
US5633155A (en) 1992-08-19 1997-05-27 Jinro Limited Expression vector for phytolacca antiviral protein and process for preparing transgenic plant transformed therewith
DE4234131C2 (en) 1992-10-09 1995-08-24 Max Planck Gesellschaft Transgenic pathogen-resistant organism
JP2651651B2 (en) 1993-04-21 1997-09-10 チッソ株式会社 Firefly luciferase gene
US5491084A (en) * 1993-09-10 1996-02-13 The Trustees Of Columbia University In The City Of New York Uses of green-fluorescent protein
US5576198A (en) 1993-12-14 1996-11-19 Calgene, Inc. Controlled expression of transgenic constructs in plant plastids
US5869255A (en) 1994-02-01 1999-02-09 The Regents Of The University Of California Probes labeled with energy transfer couples dyes exemplified with DNA fragment analysis
US5654419A (en) 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
JP3448090B2 (en) 1994-02-16 2003-09-16 浜松ホトニクス株式会社 Energy transfer detection method and apparatus
US5750870A (en) 1994-06-17 1998-05-12 Agritope, Inc. Plant genetic transformation methods and transgenic plants
US5650135A (en) 1994-07-01 1997-07-22 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive localization of a light-emitting conjugate in a mammal
KR970007864B1 (en) 1994-07-21 1997-05-17 진로 주식회사 Process of plant which expressing pip of phytolacca insularis nakai
JP3466765B2 (en) 1994-07-27 2003-11-17 キッコーマン株式会社 Biotinylated firefly luciferase, biotinylated firefly luciferase gene, novel recombinant DNA, method for producing biotinylated firefly luciferase and bioluminescence analysis method
US5625048A (en) 1994-11-10 1997-04-29 The Regents Of The University Of California Modified green fluorescent proteins
US5750868A (en) 1994-12-08 1998-05-12 Pioneer Hi-Bred International, Inc. Reversible nuclear genetic system for male sterility in transgenic plants
US5629470A (en) 1995-01-20 1997-05-13 Rutgers, The State University Of New Jersey Transgenic plants and plant cells with enhanced pathogen resistance and related methods
US5958713A (en) 1995-01-31 1999-09-28 Novo Nordisk A/S Method of detecting biologically active substances by using green fluorescent protein
KR100501838B1 (en) 1995-04-24 2005-07-21 큐비스트 파마슈티컬즈 인코포레이티드 Methods for generating and screening noverl metabolic pathways
US5674731A (en) 1995-04-27 1997-10-07 Life Technologies, Inc. Regeneration of both plant tissues and transgenic plant tissues using a new plant hormone, 5-bromoindole-3-acetic acid
US6008373A (en) 1995-06-07 1999-12-28 Carnegie Mellon University Fluorescent labeling complexes with large stokes shift formed by coupling together cyanine and other fluorochromes capable of resonance energy transfer
US6342379B1 (en) * 1995-06-07 2002-01-29 The Regents Of The University Of California Detection of transmembrane potentials by optical methods
US5654173A (en) 1996-08-23 1997-08-05 Genetics Institute, Inc. Secreted proteins and polynucleotides encoding them
US5728528A (en) 1995-09-20 1998-03-17 The Regents Of The University Of California Universal spacer/energy transfer dyes
US5968738A (en) 1995-12-06 1999-10-19 The Board Of Trustees Of The Leland Stanford Junior University Two-reporter FACS analysis of mammalian cells using green fluorescent proteins
AU710425B2 (en) 1995-12-18 1999-09-23 Washington University Method for nucleic acid analysis using fluorescence resonance energy transfer
US5874304A (en) 1996-01-18 1999-02-23 University Of Florida Research Foundation, Inc. Humanized green fluorescent protein genes and methods
US6020192A (en) 1996-01-18 2000-02-01 University Of Florida Humanized green fluorescent protein genes and methods
US6803188B1 (en) 1996-01-31 2004-10-12 The Regents Of The University Of California Tandem fluorescent protein constructs
US5804387A (en) 1996-02-01 1998-09-08 The Board Of Trustees Of The Leland Stanford Junior University FACS-optimized mutants of the green fluorescent protein (GFP)
US5863727A (en) 1996-05-03 1999-01-26 The Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US5945526A (en) 1996-05-03 1999-08-31 Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US5989835A (en) 1997-02-27 1999-11-23 Cellomics, Inc. System for cell-based screening
US5866336A (en) 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US5976796A (en) 1996-10-04 1999-11-02 Loma Linda University Construction and expression of renilla luciferase and green fluorescent protein fusion genes
TW371617B (en) 1996-10-09 1999-10-11 Of Animal And Plant Health Inspection And Quarantine Council Of Agriculture Executive Yuan Bureau Method to transplant GFP into autographa californica multiple nuclear polyhedrosis virus for inflicting pest in an attempt to detect and flow up it existence and to improve life span against UV
CN100582230C (en) 1997-09-26 2010-01-20 阿瑟西斯公司 Expression of endogenous genes by non-homologous recombination of vector constructs with cellular DNA
US6501003B1 (en) 1998-07-08 2002-12-31 Wisconsin Alumni Research Foundation Transgentic mouse expressing green fluorescent protein in glial cells
AU4992599A (en) 1998-07-13 2000-02-01 Cellomics, Inc. A system for cell-based screening
US5998146A (en) 1998-07-17 1999-12-07 Wallac Oy Homogeneous luminescence assay method based on energy transfer
AU6048599A (en) 1998-09-18 2000-04-10 Cellomics, Inc. A system for cell-based screening
JP2002525600A (en) 1998-09-22 2002-08-13 セロミックス インコーポレイテッド Miniaturized cell array method and device for cell-based screening
US5985577A (en) 1998-10-14 1999-11-16 The Trustees Of Columbia University In The City Of New York Protein conjugates containing multimers of green fluorescent protein
DK1131471T3 (en) 1998-10-30 2002-12-30 Cellomics Inc A system for cell-based screening
WO2000034323A1 (en) * 1998-12-11 2000-06-15 Clontech Laboratories, Inc. Fluorescent proteins from non-bioluminescent species of class anthozoa, genes encoding such proteins and uses thereof
WO2000034324A1 (en) * 1998-12-11 2000-06-15 Clontech Laboratories, Inc. Fluorescent proteins from non-bioluminescent species of class anthozoa, genes encoding such proteins and uses thereof
WO2000034526A1 (en) * 1998-12-11 2000-06-15 Clontech Laboratories, Inc. Fluorescent proteins from non-bioluminescent species of class anthozoa, genes encoding such proteins and uses thereof
WO2000034321A1 (en) * 1998-12-11 2000-06-15 Clontech Laboratories, Inc. Fluorescent proteins from non-bioluminescent species of class anthozoa, genes encoding such proteins and uses thereof
WO2000034318A1 (en) * 1998-12-11 2000-06-15 Clontech Laboratories, Inc. Fluorescent proteins from non-bioluminescent species of class anthozoa, genes encoding such proteins and uses thereof
WO2000034326A1 (en) * 1998-12-11 2000-06-15 Clontech Laboratories, Inc. Fluorescent proteins from non-bioluminescent species of class anthozoa, genes encoding such proteins and uses thereof
WO2000034322A1 (en) * 1998-12-11 2000-06-15 Clontech Laboratories, Inc. Fluorescent proteins from non-bioluminescent species of class anthozoa, genes encoding such proteins and uses thereof
AUPP846399A0 (en) 1999-02-02 1999-02-25 University Of Sydney, The Pigment protein from coral tissue
CA2383642C (en) 1999-10-14 2014-03-25 Clontech Laboratories, Inc. Anthozoa derived chromophores/fluorophores and methods for using the same
AU1446801A (en) 1999-10-29 2001-05-14 Rutgers, The State University Of New Jersey Renilla reniformis green fluorescent protein
DK1228233T3 (en) 1999-11-10 2007-05-14 Rigel Pharmaceuticals Inc Methods and compositions comprising Renfla Green Fluorescent Proteins (GFP)

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19718640A1 (en) * 1997-05-02 1999-07-22 Joerg Wiedenmann Protein derived from Anemonia useful in gene technology and molecular biology
WO1999049019A2 (en) * 1998-03-27 1999-09-30 Prolume, Ltd. Luciferases, fluorescent proteins, nucleic acids encoding the luciferases and fluorescent proteins and the use thereof in diagnostics

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DOVE SOPHIE G ET AL: "Isolation and partial characterization of the pink and blue pigments of pocilloporid and acroporid corals.", BIOLOGICAL BULLETIN (WOODS HOLE), vol. 189, no. 3, 1995, pages 288 - 297, XP000974081, ISSN: 0006-3185 *
MATZ M V ET AL: "FLUORESCENT PROTEINS FROM NONBIOLUMINESCENT ANTHOZOA SPECIES", NATURE BIOTECHNOLOGY,NATURE PUBLISHING,US, vol. 17, no. 10, 1999, pages 969 - 973, XP000882891, ISSN: 1087-0156 *
See also references of EP1305412A2 *
TSIEN R Y: "THE GREEN FLUORESCENT PROTEIN", ANNUAL REVIEW OF BIOCHEMISTRY,PALTO ALTO, CA,US, vol. 67, 1998, pages 509 - 544, XP000946725 *
TSIEN R.Y.: "ROSY DAWN FOR FLUORESCENT PROTEINS", NATURE BIOTECHNOLOGY, vol. 17, no. 10, October 1999 (1999-10-01), pages 956 - 957, XP002159040 *
WARD W W ET AL: "AN ENERGY TRANSFER PROTEIN IN COELENTERATE BIOLUMINESCENCE CHARACTERIZATION OF THE RENILLA GREEN-FLUORESCENT PROTEIN", JOURNAL OF BIOLOGICAL CHEMISTRY. (MICROFILMS),US,AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, vol. 254, no. 3, 10 February 1979 (1979-02-10), pages 781 - 788, XP002030243 *

Also Published As

Publication number Publication date
US20070134703A1 (en) 2007-06-14
US7166444B2 (en) 2007-01-23
AU783767B2 (en) 2005-12-01
US7344862B2 (en) 2008-03-18
CA2383642A1 (en) 2001-04-19
EP1305412A2 (en) 2003-05-02
US7442522B2 (en) 2008-10-28
US20070231893A1 (en) 2007-10-04
US20070148735A1 (en) 2007-06-28
EP2308974A2 (en) 2011-04-13
AU1086701A (en) 2001-04-23
JP2003527833A (en) 2003-09-24
WO2001027150A2 (en) 2001-04-19
US20020197676A1 (en) 2002-12-26
US7442521B2 (en) 2008-10-28
EP2308974A3 (en) 2011-08-10
CA2383642C (en) 2014-03-25

Similar Documents

Publication Publication Date Title
WO2001027150A3 (en) Anthozoa derived chromo/fluoroproteins and methods for using the same
CA2165566A1 (en) Conotoxins having acetylcholin receptor binding properties
WO1999061619A8 (en) Cell cycle genes, proteins and uses thereof
WO2003042401A3 (en) Novel chromophores/fluorophores and methods for using the same
WO2004020605A3 (en) Circular nucleic acid vectors, and methods for making and using the same
CA2418961A1 (en) Anti-il-12 antibodies, compositions, methods and uses
EP2105449A3 (en) Antagonistic selective binding agents of osteoprotegerin binding protein
CA2413156A1 (en) Expression vectors
WO2005003296A3 (en) Albumin fusion proteins
WO2003020932A1 (en) Novel secretory proteins and dna thereof
WO2003030821A3 (en) Albumin fusion proteins
WO2002062999A3 (en) Proteins and nucleic acids encoding same
WO2001096373A3 (en) Fluorescent timer proteins and methods for their use
CA2281895A1 (en) Ikb kinases
WO2003065003A3 (en) Nuclear matrix protein alterations associated with colon cancer and colon metastasis to the liver, and uses thereof
WO1999067382A3 (en) Angiopoietin-like growth factor sequences
WO2002030965A3 (en) Nucleic acids encoding stichodactylidae chromoproteins
CA2294566A1 (en) Ikk-.alpha. proteins, nucleic acids and methods
WO2001034791A3 (en) Chemoreceptors in plant parasitic nematodes
AU2003287115A1 (en) Fluorescent proteins from copepoda species and methods for using same
WO2003062270A8 (en) Fluorescent protein from aequorea coerulscens and uses therof
AU2003285844A1 (en) Fluorescent proteins and chromoproteins from non-aequorea hydrozoa species and methods for using same
WO2002068459A3 (en) Non aggregating fluorescent proteins and methods for using the same
WO2003062378A3 (en) Methods of random mutagenesis and methods of modifying nucleic acids using translesion dna polymerases
EP1132397A4 (en) 'SENSOR PROTEIN' AND ITS USE

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US US US US US US US US US US US US US

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US US US US US US US US US US US US US

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

WWE Wipo information: entry into national phase

Ref document number: 2383642

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 517548

Country of ref document: NZ

Ref document number: 10867/01

Country of ref document: AU

ENP Entry into the national phase

Ref country code: JP

Ref document number: 2001 530368

Kind code of ref document: A

Format of ref document f/p: F

WWE Wipo information: entry into national phase

Ref document number: 2000972169

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWP Wipo information: published in national office

Ref document number: 2000972169

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 517548

Country of ref document: NZ

WWG Wipo information: grant in national office

Ref document number: 517548

Country of ref document: NZ

WWG Wipo information: grant in national office

Ref document number: 10867/01

Country of ref document: AU