WO2001027331A3 - Detection of nucleic acid heteroduplex molecules by anion-exchange chromatography - Google Patents

Detection of nucleic acid heteroduplex molecules by anion-exchange chromatography Download PDF

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Publication number
WO2001027331A3
WO2001027331A3 PCT/US2000/028441 US0028441W WO0127331A3 WO 2001027331 A3 WO2001027331 A3 WO 2001027331A3 US 0028441 W US0028441 W US 0028441W WO 0127331 A3 WO0127331 A3 WO 0127331A3
Authority
WO
WIPO (PCT)
Prior art keywords
anion
exchange chromatography
detection
nucleic acid
heteroduplexes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2000/028441
Other languages
French (fr)
Other versions
WO2001027331A2 (en
Inventor
Paul D Taylor
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Precipio Inc
Original Assignee
Transgenomic Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Transgenomic Inc filed Critical Transgenomic Inc
Priority to AU10856/01A priority Critical patent/AU1085601A/en
Priority to JP2001529461A priority patent/JP2004514874A/en
Priority to DE60040232T priority patent/DE60040232D1/en
Priority to EP00972158A priority patent/EP1226275B1/en
Publication of WO2001027331A2 publication Critical patent/WO2001027331A2/en
Anticipated expiration legal-status Critical
Publication of WO2001027331A3 publication Critical patent/WO2001027331A3/en
Ceased legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
    • B01J41/20—Anion exchangers for chromatographic processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/366—Ion-pair, e.g. ion-pair reversed phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)

Abstract

The present invention describes a method for separating heteroduplex and homoduplex DNA molecules in a mixture. In the method, such a mixture is applied to an anion-exchange chromatography medium. The heteroduplex and homoduplex molecules are eluted with a mobile phase containing an eluting salt, including an anion and a cation, a buffer, and preferably including an organic solvent. The eluting is carried out under conditions effective to at least partially denature the heteroduplexes (e.g., thermal or chemical denaturing) resulting in the separation of the heteroduplexes from the homoduplexes. The method has many applications including, but not limited to, detecting mutations and comparative DNA sequencing.
PCT/US2000/028441 1999-10-12 2000-10-12 Detection of nucleic acid heteroduplex molecules by anion-exchange chromatography Ceased WO2001027331A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU10856/01A AU1085601A (en) 1999-10-12 2000-10-12 Detection of nucleic acid heteroduplex molecules by anion-exchange chromatography
JP2001529461A JP2004514874A (en) 1999-10-12 2000-10-12 Detection of nucleic acid heteroduplex molecules by anion-exchange chromatography
DE60040232T DE60040232D1 (en) 1999-10-12 2000-10-12 DETECTION OF NUCLEIC ACID HETERODUPLEX MOLECULES USING ANION EXCHANGE CHROMATOGRAPHY
EP00972158A EP1226275B1 (en) 1999-10-12 2000-10-12 Detection of nucleic acid heteroduplex molecules by anion-exchange chromatography

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US15874799P 1999-10-12 1999-10-12
US60/158,747 1999-10-12
US19465200P 2000-04-04 2000-04-04
US60/194,652 2000-04-04

Publications (2)

Publication Number Publication Date
WO2001027331A2 WO2001027331A2 (en) 2001-04-19
WO2001027331A3 true WO2001027331A3 (en) 2002-05-16

Family

ID=26855339

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/028441 Ceased WO2001027331A2 (en) 1999-10-12 2000-10-12 Detection of nucleic acid heteroduplex molecules by anion-exchange chromatography

Country Status (6)

Country Link
EP (1) EP1226275B1 (en)
JP (1) JP2004514874A (en)
AT (1) ATE408026T1 (en)
AU (1) AU1085601A (en)
DE (1) DE60040232D1 (en)
WO (1) WO2001027331A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6969587B2 (en) 2000-04-04 2005-11-29 Taylor Paul D Detection of nucleic acid heteroduplex molecules by anion-exchange chromatography
CN1279903C (en) * 2002-08-23 2006-10-18 江苏康缘药业股份有限公司 Preparation containing ginkgolide and its preparation process
GB2443505B (en) 2006-09-26 2008-12-31 Ge Healthcare Bio Sciences Nucleic acid purification method
JP6061381B2 (en) * 2011-01-12 2017-01-18 積水メディカル株式会社 Single nucleotide polymorphism detection method
US9481881B2 (en) 2011-01-12 2016-11-01 Sekisui Medical Co., Ltd. Eluent for ion-exchange chromatography, and method of analyzing nucleic acid chains
WO2012133834A1 (en) * 2011-03-31 2012-10-04 積水メディカル株式会社 Sample nucleic acid for single nucleotide polymorphism detection purposes, pcr primer for preparing sample for single nucleotide polymorphism detection purposes, and method for preparing sample for single nucleotide polymorphism detection purposes which can be used in ion exchange chromatographic analysis
EP3850096A1 (en) 2018-09-11 2021-07-21 Amgen Inc. Purification methods for guanine-rich oligonucleotides
CN111208215B (en) * 2018-11-21 2023-05-12 四川科伦药物研究院有限公司 Method for detecting impurity 2-mercaptobenzothiazole in ceftriaxone sodium
CN110484016B (en) * 2019-07-31 2021-01-05 辽宁科技大学 Yellow fluorescent dye and preparation method and application thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0507591A2 (en) * 1991-04-03 1992-10-07 The Perkin-Elmer Corporation Anion-exchange separation of nucleic acids
US5795976A (en) * 1995-08-08 1998-08-18 The Board Of Trustees Of The Leland Stanford Junior University Detection of nucleic acid heteroduplex molecules by denaturing high-performance liquid chromatography and methods for comparative sequencing
WO1999009203A1 (en) * 1997-08-18 1999-02-25 Transgenomic, Inc. Chromatographic method for mutation detection using mutation site specifically acting enzymes
WO1999022839A1 (en) * 1997-10-30 1999-05-14 Transgenomic, Inc. Method for high resolution liquid chromatographic separation of polynucleotides

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0507591A2 (en) * 1991-04-03 1992-10-07 The Perkin-Elmer Corporation Anion-exchange separation of nucleic acids
US5795976A (en) * 1995-08-08 1998-08-18 The Board Of Trustees Of The Leland Stanford Junior University Detection of nucleic acid heteroduplex molecules by denaturing high-performance liquid chromatography and methods for comparative sequencing
WO1999009203A1 (en) * 1997-08-18 1999-02-25 Transgenomic, Inc. Chromatographic method for mutation detection using mutation site specifically acting enzymes
WO1999022839A1 (en) * 1997-10-30 1999-05-14 Transgenomic, Inc. Method for high resolution liquid chromatographic separation of polynucleotides

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
HUBER C G: "Micropellicular stationary phases for high-performance liquid chromatography of double-stranded DNA", JOURNAL OF CHROMATOGRAPHY A, ELSEVIER SCIENCE, NL, vol. 806, no. 1, 8 May 1998 (1998-05-08), pages 3 - 30, XP004121165, ISSN: 0021-9673 *

Also Published As

Publication number Publication date
ATE408026T1 (en) 2008-09-15
DE60040232D1 (en) 2008-10-23
AU1085601A (en) 2001-04-23
JP2004514874A (en) 2004-05-20
WO2001027331A2 (en) 2001-04-19
EP1226275B1 (en) 2008-09-10
EP1226275A2 (en) 2002-07-31

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