WO2001029241A3 - Methods and vectors for site-specific recombination in plant cell plastids - Google Patents

Methods and vectors for site-specific recombination in plant cell plastids Download PDF

Info

Publication number
WO2001029241A3
WO2001029241A3 PCT/US2000/028620 US0028620W WO0129241A3 WO 2001029241 A3 WO2001029241 A3 WO 2001029241A3 US 0028620 W US0028620 W US 0028620W WO 0129241 A3 WO0129241 A3 WO 0129241A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
specific recombination
plant cell
site
vectors
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2000/028620
Other languages
French (fr)
Other versions
WO2001029241A2 (en
Inventor
Jeffrey M Staub
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Monsanto Co
Original Assignee
Calgene LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Calgene LLC filed Critical Calgene LLC
Priority to CA002387876A priority Critical patent/CA2387876A1/en
Priority to EP00972211A priority patent/EP1222296A2/en
Priority to BR0014858-0A priority patent/BR0014858A/en
Priority to AU10907/01A priority patent/AU1090701A/en
Publication of WO2001029241A2 publication Critical patent/WO2001029241A2/en
Publication of WO2001029241A3 publication Critical patent/WO2001029241A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Novel compositions and methods useful for genetic engineering of plant cells are provided. In particular, plastid constructs comprising recombining sites are provided. Such constructs find use in methods for site specific recombination in plant cell plastids. Plant cells and plants comprising the constructs described herein, as well as plant cells and plants produced by the methods of the present invention are of interest.
PCT/US2000/028620 1999-10-15 2000-10-16 Methods and vectors for site-specific recombination in plant cell plastids Ceased WO2001029241A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002387876A CA2387876A1 (en) 1999-10-15 2000-10-16 Methods and vectors for site-specific recombination in plant cell plastids
EP00972211A EP1222296A2 (en) 1999-10-15 2000-10-16 Methods and vectors for site-specific recombination in plant cell plastids
BR0014858-0A BR0014858A (en) 1999-10-15 2000-10-16 Methods and vectors for recombination of specific site in plant cell plastids
AU10907/01A AU1090701A (en) 1999-10-15 2000-10-16 Methods and vectors for site-specific recombination in plant cell plastids

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US15987699P 1999-10-15 1999-10-15
US60/159,876 1999-10-15
US22554200P 2000-08-16 2000-08-16
US60/225,542 2000-08-16

Publications (2)

Publication Number Publication Date
WO2001029241A2 WO2001029241A2 (en) 2001-04-26
WO2001029241A3 true WO2001029241A3 (en) 2001-11-29

Family

ID=26856401

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/028620 Ceased WO2001029241A2 (en) 1999-10-15 2000-10-16 Methods and vectors for site-specific recombination in plant cell plastids

Country Status (5)

Country Link
EP (1) EP1222296A2 (en)
AU (1) AU1090701A (en)
BR (1) BR0014858A (en)
CA (1) CA2387876A1 (en)
WO (1) WO2001029241A2 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7119250B2 (en) 1997-06-03 2006-10-10 The University Of Chicago Plant centromere compositions
US7193128B2 (en) 1997-06-03 2007-03-20 Chromatin, Inc. Methods for generating or increasing revenues from crops
US8937216B2 (en) 2007-03-09 2015-01-20 Monsanto Technology Llc Method of meristem excision and transformation

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7235716B2 (en) 1997-06-03 2007-06-26 Chromatin, Inc. Plant centromere compositions
US7227057B2 (en) 1997-06-03 2007-06-05 Chromatin, Inc. Plant centromere compositions
ES2558160T3 (en) 1998-12-09 2016-02-02 Phyton Holdings, Llc Glycoproteins that have human-type glycosylation
ATE478150T1 (en) 1999-10-26 2010-09-15 Stichting Dienst Landbouwkundi MAMMAL GLYCOSYLATION IN PLANTS
US7608445B1 (en) 1999-12-29 2009-10-27 Verenium Corporation Nitrilases, nucleic acids encoding them and methods for making and using them
US7300775B2 (en) 1999-12-29 2007-11-27 Verenium Corporation Methods for producing α-substituted carboxylic acids using nitrilases and strecker reagents
US7521216B2 (en) 1999-12-29 2009-04-21 Verenium Corporation Nitrilases and methods for making and using them
EP1276884A2 (en) * 2000-04-20 2003-01-22 Btg International Limited Transgenic plants
BRPI0206546B1 (en) 2001-01-19 2015-07-21 Phyton Holdings Llc Method for secretory production of human-type sugar chain glycoprotein using plant cell
EP1461439A1 (en) * 2001-12-20 2004-09-29 Sungene GmbH & Co. KGaA Method for the transformation of vegetable plastids
EP1458875A2 (en) * 2001-12-20 2004-09-22 Sungene GmbH & Co. KGaA Methods for the transformation of vegetal plastids
KR101070518B1 (en) 2002-03-19 2011-10-05 스티칭 디엔스트 랜드보위쿤디그 온데조에크 Gntiii expression in plants
CA2478297C (en) 2002-03-19 2013-05-14 Plant Research International B.V. Optimizing glycan processing in plants
AU2003251523A1 (en) 2002-06-13 2003-12-31 Diversa Corporation Processes for making (r)-ethyl 4-cyano-3-hydroxybutyric acid
CL2003002461A1 (en) 2002-11-27 2005-01-07 Dow Chemical Company Agroscien IMMUNOGLOBULIN THAT UNDERSTANDS AT LEAST ONE AFUCOSILATED GLICAN, COMPOSITION THAT CONTAINS IT, NUCLEOTIDIC SEQUENCE AND VECTOR THAT UNDERSTANDS IT, PROCEDURE TO PRODUCE IMMUNOGLOBULIN SAID IN PLANTS.
CA2517856A1 (en) 2003-03-03 2004-09-16 Rutgers, The State University Of New Jersey Removal of plastid sequences by transiently expressed site-specific recombinases
US7150993B2 (en) 2003-08-05 2006-12-19 Monsanto Technology Llc Method for excision of plant embryos for transformation
US7560611B2 (en) 2003-08-05 2009-07-14 Monsanto Technology Llc Method and apparatus for substantially isolating plant tissues
GB2447416B (en) * 2006-12-15 2010-08-25 Uni I Stavanger Methods and vectors for transformation of plastids and marker excision using site-specific recombination
EP2115153B1 (en) 2007-03-01 2013-06-05 BP Corporation North America Inc. Nitrilases, nucleic acids encoding them and methods for making and using them
KR101426576B1 (en) 2007-04-17 2014-08-07 스티칭 디엔스트 랜드보위쿤디그 온데조에크 Mammalian-type glycosylation in plants by expression of non-mammalian glycosyltransferases
EP2456894A4 (en) 2009-07-23 2013-09-25 Chromatin Inc Sorghum centromere sequences and minichromosomes

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997041228A2 (en) * 1996-05-01 1997-11-06 Pioneer Hi-Bred International, Inc. Use of the green fluorescent protein as a screenable marker for plant transformation
WO2001021768A1 (en) * 1999-09-21 2001-03-29 Rutgers, The State University Of New Jersey Site-specific recombination system to manipulate the plastid genome of higher plants

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997041228A2 (en) * 1996-05-01 1997-11-06 Pioneer Hi-Bred International, Inc. Use of the green fluorescent protein as a screenable marker for plant transformation
WO2001021768A1 (en) * 1999-09-21 2001-03-29 Rutgers, The State University Of New Jersey Site-specific recombination system to manipulate the plastid genome of higher plants

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DALE E C ET AL: "GENE TRANSFER WITH SUBSEQUENT REMOVAL OF THE SELECTION GENE FROM THE HOST GENOME", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA,US,NATIONAL ACADEMY OF SCIENCE. WASHINGTON, vol. 88, 1 December 1991 (1991-12-01), pages 10558 - 10562, XP002043803, ISSN: 0027-8424 *
DALE ET AL: "Intra- and intermolecular site-specific recombination in plant cells mediated by bacteriophage P1 recombinase", GENE,NL,ELSEVIER BIOMEDICAL PRESS. AMSTERDAM, vol. 91, 1990, pages 79 - 85, XP002094597, ISSN: 0378-1119 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7119250B2 (en) 1997-06-03 2006-10-10 The University Of Chicago Plant centromere compositions
US7193128B2 (en) 1997-06-03 2007-03-20 Chromatin, Inc. Methods for generating or increasing revenues from crops
US7456013B2 (en) 1997-06-03 2008-11-25 Chromatin, Inc. Plant centromere compositions
US8937216B2 (en) 2007-03-09 2015-01-20 Monsanto Technology Llc Method of meristem excision and transformation
US9006513B2 (en) 2007-03-09 2015-04-14 Monsanto Technology Llc Preparation and use of plant embryo explants for transformation
US11566252B2 (en) 2007-03-09 2023-01-31 Monsanto Technology, Llc Preparation and use of plant embryo explants for transformation

Also Published As

Publication number Publication date
WO2001029241A2 (en) 2001-04-26
BR0014858A (en) 2002-07-16
AU1090701A (en) 2001-04-30
CA2387876A1 (en) 2001-04-26
EP1222296A2 (en) 2002-07-17

Similar Documents

Publication Publication Date Title
WO2001029241A3 (en) Methods and vectors for site-specific recombination in plant cell plastids
WO2002074969A3 (en) Matrix attachment regions and methods for use thereof
WO2000063391A3 (en) Nucleic acid sequences to proteins involved in tocopherol synthesis
WO2005005643A3 (en) Chloroplast transformation of duckweed
EP1574573A3 (en) Compositions and methods for genetic modification of plants
WO2002042424A3 (en) Seed-preferred regulatory elements and uses thereof
PT1032680E (en) A NEW METHOD FOR THE INTEGRATION OF STRANGE DNA IN EUCARIOTIC GENOME
WO2002098210A3 (en) Chromosome doubling method
WO2002061072A3 (en) Transgenic plants containing altered levels of steroid compounds
IL110781A0 (en) Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
WO2001044457A3 (en) Dna constructs for expression of heterologous polypeptides in plants
WO2004005480A3 (en) Plastid genetic engineering via somatic embryogenesis
WO2001077161A3 (en) Gtp binding stress-related proteins and methods of use in plants
IL147385A0 (en) Methods and compositions for directed cloning and subcloning using homologous recombination
WO2001044459A3 (en) Method to enhance agrobacterium-mediated transformation of plants
WO2001025460A3 (en) Compositions and methods for plant genetic modification
WO2003083086A3 (en) High level expression of immunogenic proteins in the plastids of higher plants
WO1996007749A3 (en) Methods and compositions for inhibiting production of replication competent virus
WO2006055931A3 (en) Vectors for stable gene expression
AU2002252984A1 (en) Stress resistance as a selectable marker for transgenic plants
WO1999060108A3 (en) Transgenic animals produced by homologous sequence targeting
WO2004035797A3 (en) Tissue-specific promoters from plants
WO2001018192A3 (en) Synthetic vectors, transgenic plants containing them, and methods for obtaining them
PT1322756E (en) Manipulation of plant senescence using an i myb /i gene promoter and cytokinin biosynthesis genes
WO1999053037A3 (en) Optimized nucleotide sequence encoding organophosphorous hydrolase and methods of use for same

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

WWE Wipo information: entry into national phase

Ref document number: 10907/01

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 2387876

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 2000972211

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2000972211

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWW Wipo information: withdrawn in national office

Ref document number: 2000972211

Country of ref document: EP

NENP Non-entry into the national phase

Ref country code: JP