WO2001032898A3 - Recombinant sendai virus vector for introducing exogenous genes to airway epithelia - Google Patents

Recombinant sendai virus vector for introducing exogenous genes to airway epithelia Download PDF

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Publication number
WO2001032898A3
WO2001032898A3 PCT/JP2000/007737 JP0007737W WO0132898A3 WO 2001032898 A3 WO2001032898 A3 WO 2001032898A3 JP 0007737 W JP0007737 W JP 0007737W WO 0132898 A3 WO0132898 A3 WO 0132898A3
Authority
WO
WIPO (PCT)
Prior art keywords
vector
sendai virus
introducing exogenous
exogenous genes
virus vector
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2000/007737
Other languages
French (fr)
Other versions
WO2001032898A2 (en
Inventor
Yoshikazu Yonemitsu
Mamoru Hasegawa
Eric Wfw Alton
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Dnavec Research Inc
Original Assignee
Dnavec Research Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dnavec Research Inc filed Critical Dnavec Research Inc
Priority to HK02108796.7A priority Critical patent/HK1047137B/en
Priority to CA2389743A priority patent/CA2389743C/en
Priority to JP2001535580A priority patent/JP4838962B2/en
Priority to US10/111,356 priority patent/US7314614B1/en
Priority to AU10551/01A priority patent/AU1055101A/en
Priority to EP00971756A priority patent/EP1228232B1/en
Priority to DE60033048T priority patent/DE60033048T2/en
Priority to DK00971756T priority patent/DK1228232T3/en
Publication of WO2001032898A2 publication Critical patent/WO2001032898A2/en
Publication of WO2001032898A3 publication Critical patent/WO2001032898A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/18011—Paramyxoviridae
    • C12N2760/18811—Sendai virus
    • C12N2760/18841—Use of virus, viral particle or viral elements as a vector
    • C12N2760/18843—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Virology (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Physics & Mathematics (AREA)
  • Public Health (AREA)
  • Pulmonology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Provided are a recombinant Sendai virus vector for introducing exogenous genes to airway epithelia and a method for introducing exogenous genes using the vector. The recombinant Sendai virus vector enables efficient gene transfer to native mucus-layered airway epithelial cells by briefly contacting the vector with the cells. Furthermore, the vector can introduce genes to not only apical surfaces but also submucosal glands where CFTR primarily expresses. The vector can thus be used for gene therapy of CF, a CFTR-deficient disease.
PCT/JP2000/007737 1999-11-02 2000-11-02 Recombinant sendai virus vector for introducing exogenous genes to airway epithelia Ceased WO2001032898A2 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
HK02108796.7A HK1047137B (en) 1999-11-02 2000-11-02 Recombinant sendai virus vector for introducing exogenous genes to airway epithelia
CA2389743A CA2389743C (en) 1999-11-02 2000-11-02 Recombinant sendai virus vector for introducing exogenous genes to airway epithelia
JP2001535580A JP4838962B2 (en) 1999-11-02 2000-11-02 Recombinant Sendai virus vector for exogenous gene transfer into airway epithelial cells
US10/111,356 US7314614B1 (en) 1999-11-02 2000-11-02 Recombinant sendai virus vector for introducing exogenous genes to airway epithelia
AU10551/01A AU1055101A (en) 1999-11-02 2000-11-02 Recombinant sendai virus vector for introducing exogenous genes to airway epithelia
EP00971756A EP1228232B1 (en) 1999-11-02 2000-11-02 Recombinant sendai virus vector for introducing exogenous genes to airway epithelia
DE60033048T DE60033048T2 (en) 1999-11-02 2000-11-02 RECOMBINANT SENDAIVIRUS VECTOR FOR THE INTRODUCTION OF FOREIGN GENES TO THE EPITHELLE OF THE RESPIRATORY PATHS
DK00971756T DK1228232T3 (en) 1999-11-02 2000-11-02 Recombinant Sendai virus vector to introduce exogenous genes into airway epithelium

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US16305599P 1999-11-02 1999-11-02
US60/163,055 1999-11-02
JP35921899 1999-12-17
JP11-359218 1999-12-17

Publications (2)

Publication Number Publication Date
WO2001032898A2 WO2001032898A2 (en) 2001-05-10
WO2001032898A3 true WO2001032898A3 (en) 2001-09-07

Family

ID=26580919

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2000/007737 Ceased WO2001032898A2 (en) 1999-11-02 2000-11-02 Recombinant sendai virus vector for introducing exogenous genes to airway epithelia

Country Status (11)

Country Link
EP (1) EP1228232B1 (en)
KR (1) KR100772276B1 (en)
CN (1) CN1252274C (en)
AT (1) ATE351915T1 (en)
AU (1) AU1055101A (en)
CA (1) CA2389743C (en)
DE (1) DE60033048T2 (en)
DK (1) DK1228232T3 (en)
ES (1) ES2280254T3 (en)
HK (1) HK1047137B (en)
WO (1) WO2001032898A2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7226786B2 (en) 1999-05-18 2007-06-05 Dnavec Research Inc. Envelope gene-deficient Paramyxovirus vector
US8211868B2 (en) 2000-11-27 2012-07-03 Dnavec Research Inc. Paramyxovirus vector encoding angiogenesis gene and use thereof

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2003092738A1 (en) * 2002-04-30 2005-09-08 株式会社ディナベック研究所 Drug or gene delivery composition with reduced hemagglutinin activity
CA2627485C (en) * 2005-10-28 2017-02-21 Katsuyuki Mitomo Gene transfer into airway epithelial stem cell by using lentiviral vector pseudotyped with rna virus or dna virus spike protein
US9145564B2 (en) * 2007-04-13 2015-09-29 National Institute Of Advanced Industrial Science And Technology Persistently infective Sendai virus vector
EP2617438B1 (en) 2010-09-15 2020-02-19 Tokyo Women's Medical University Middle ear mucous membrane-like cell sheet, production method therefor and utilization thereof

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK0863202T3 (en) 1995-11-01 2010-09-27 Dnavec Research Inc Recombinant Sendai virus

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
HASAN M. K. ET AL.: "CREATION OF AN INFECTIOUS RECOMBINANT SENDAI VIRUS EXPRESSING THE FIRELY LUCIFERASE GENE FROM THE 3' PROXIMAL FIRST LOCUS", JOURNAL OF GENERAL VIROLOGY, vol. 78, no. 11, 1997, pages 2813 - 2820, XP000886572, ISSN: 0022-1317 *
SAEKI Y. ET AL.: "Development and characterization of cationic liposomes conjugated with HVJ (Sendai virus): reciprocal effect of cationic lipid for in vitro and in vivo gene transfer.", HUMAN GENE THERAPY, vol. 8, 20 November 1997 (1997-11-20), pages 2133 - 2141, XP000992958 *
SAKAI Y. ET AL.: "ACCOMMODATION OF FOREIGN GENES INTO THE SENDAI VIRUS GENOME: SIZES OF INSERTED GENES AND VIRAL REPLICATION", FEBS LETTERS, vol. 456, 6 August 1999 (1999-08-06), pages 221 - 226, XP000887286, ISSN: 0014-5793 *
YONEMITSU Y. ET AL.: "Efficient gene transfer to airway epithelium using recombinant Sendai virus.", NATURE BIOTECHNOLOGY, vol. 18, no. 9, September 2000 (2000-09-01), pages 970 - 973, XP002165781, ISSN: 1087-0156 *
YONEMITSU Y. ET AL.: "HVJ (Sendai virus)-cationic liposomes: A novel and potentially effective liposome-mediated technique for gene transfer to the airway epithelium.", GENE THERAPY, vol. 4, no. 7, 1997, pages 631 - 638, XP000992923, ISSN: 0969-7128 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7226786B2 (en) 1999-05-18 2007-06-05 Dnavec Research Inc. Envelope gene-deficient Paramyxovirus vector
US8211868B2 (en) 2000-11-27 2012-07-03 Dnavec Research Inc. Paramyxovirus vector encoding angiogenesis gene and use thereof

Also Published As

Publication number Publication date
HK1047137A1 (en) 2003-02-07
KR100772276B1 (en) 2007-11-01
KR20020057990A (en) 2002-07-12
DE60033048T2 (en) 2007-08-23
ES2280254T3 (en) 2007-09-16
DE60033048D1 (en) 2007-03-08
HK1047137B (en) 2007-04-13
ATE351915T1 (en) 2007-02-15
CN1252274C (en) 2006-04-19
WO2001032898A2 (en) 2001-05-10
DK1228232T3 (en) 2007-03-26
EP1228232A2 (en) 2002-08-07
CA2389743C (en) 2010-02-23
CN1409767A (en) 2003-04-09
EP1228232B1 (en) 2007-01-17
CA2389743A1 (en) 2001-05-10
AU1055101A (en) 2001-05-14

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