WO2001036460A3 - Methods and means for regulation of gene expression - Google Patents

Methods and means for regulation of gene expression Download PDF

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Publication number
WO2001036460A3
WO2001036460A3 PCT/IB2000/001828 IB0001828W WO0136460A3 WO 2001036460 A3 WO2001036460 A3 WO 2001036460A3 IB 0001828 W IB0001828 W IB 0001828W WO 0136460 A3 WO0136460 A3 WO 0136460A3
Authority
WO
WIPO (PCT)
Prior art keywords
polypeptide component
regulation
methods
polypeptide
gene expression
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IB2000/001828
Other languages
French (fr)
Other versions
WO2001036460A2 (en
Inventor
Francesco Raffaele De
Riccardo Cortese
Petra Neddermann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Istituto di Ricerche di Biologia Molecolare P Angeletti SpA
Original Assignee
Istituto di Ricerche di Biologia Molecolare P Angeletti SpA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Istituto di Ricerche di Biologia Molecolare P Angeletti SpA filed Critical Istituto di Ricerche di Biologia Molecolare P Angeletti SpA
Priority to CA002391971A priority Critical patent/CA2391971A1/en
Priority to AU17230/01A priority patent/AU1723001A/en
Priority to EP00979852A priority patent/EP1232271A2/en
Publication of WO2001036460A2 publication Critical patent/WO2001036460A2/en
Publication of WO2001036460A3 publication Critical patent/WO2001036460A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/71—Fusion polypeptide containing domain for protein-protein interaction containing domain for transcriptional activaation, e.g. VP16
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A transcriptional activator which comprises a fusion protein, the fusion protein comprising at least two polypeptide components, a first polypeptide component which binds in a sequence specific manner to an operator sequence in DNA, and a second polypeptide component, comprising a Lux-R transcription factor regulatory domain which binds cognate Acy1HSL or an analogue thereof, whereby upon binding of the Acy1HSL or analogue the DNA binding function of the first polypeptide component is activated; the transcriptional activator additionally comprising a third polypeptide component which activates transcription in eukaryotic cells.
PCT/IB2000/001828 1999-11-17 2000-11-17 Methods and means for regulation of gene expression Ceased WO2001036460A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CA002391971A CA2391971A1 (en) 1999-11-17 2000-11-17 Methods and means for regulation of gene expression
AU17230/01A AU1723001A (en) 1999-11-17 2000-11-17 Methods and means for regulation of gene expression
EP00979852A EP1232271A2 (en) 1999-11-17 2000-11-17 Methods and means for regulation of gene expression

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9927191.8A GB9927191D0 (en) 1999-11-17 1999-11-17 Methods and means for regulation of gene expression
GB9927191.8 1999-11-17

Publications (2)

Publication Number Publication Date
WO2001036460A2 WO2001036460A2 (en) 2001-05-25
WO2001036460A3 true WO2001036460A3 (en) 2002-01-03

Family

ID=10864679

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2000/001828 Ceased WO2001036460A2 (en) 1999-11-17 2000-11-17 Methods and means for regulation of gene expression

Country Status (5)

Country Link
EP (1) EP1232271A2 (en)
AU (1) AU1723001A (en)
CA (1) CA2391971A1 (en)
GB (1) GB9927191D0 (en)
WO (1) WO2001036460A2 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ITMI20090946A1 (en) * 2009-05-28 2010-11-29 Novartis Ag EXPRESSION OF RECOMBINANT PROTEINS
US9428845B1 (en) 2010-12-28 2016-08-30 Warp Drive Bio, Inc. Identifying new therapeutic agents
TW201629069A (en) 2015-01-09 2016-08-16 霍普驅動生物科技股份有限公司 Compounds that participate in synergistic combination and their uses
EP3247378B8 (en) 2015-01-09 2023-08-23 Revolution Medicines, Inc. Macrocyclic compounds that participate in cooperative binding and medical uses thereof
EP4242304A3 (en) 2015-10-01 2024-02-07 Revolution Medicines, Inc. Methods and reagents for analyzing protein-protein interfaces
CN109641062A (en) * 2016-04-12 2019-04-16 华普卓威生物公司 For generating the composition and method of compound
US11479797B2 (en) 2016-10-28 2022-10-25 Ginkgo Bioworks, Inc. Compositions and methods for the production of compounds
WO2021050789A1 (en) 2019-09-10 2021-03-18 Obsidian Therapeutics, Inc. Ca2-il15 fusion proteins for tunable regulation
AU2020377925A1 (en) 2019-11-04 2022-05-05 Revolution Medicines, Inc. Ras inhibitors
TW202132316A (en) 2019-11-04 2021-09-01 美商銳新醫藥公司 Ras inhibitors
IL322454A (en) 2019-11-04 2025-09-01 Revolution Medicines Inc Ras inhibitors
CA3194067A1 (en) 2020-09-15 2022-03-24 Revolution Medicines, Inc. Ras inhibitors
KR20240017811A (en) 2021-05-05 2024-02-08 레볼루션 메디슨즈, 인크. RAS inhibitors for the treatment of cancer
AR127308A1 (en) 2021-10-08 2024-01-10 Revolution Medicines Inc RAS INHIBITORS

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5196318A (en) * 1990-06-26 1993-03-23 The Texas A&M University System Precisely regulated expression of deleterious genes
WO1994028135A1 (en) * 1993-06-01 1994-12-08 University Of Warwick Process for activating gene expression in bacteria
WO2000009704A1 (en) * 1998-08-13 2000-02-24 Syngenta Limited Gene switch
WO2001002593A2 (en) * 1999-07-01 2001-01-11 Calgene Llc Control of gene expression in eukaryotic cells

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5196318A (en) * 1990-06-26 1993-03-23 The Texas A&M University System Precisely regulated expression of deleterious genes
WO1994028135A1 (en) * 1993-06-01 1994-12-08 University Of Warwick Process for activating gene expression in bacteria
WO2000009704A1 (en) * 1998-08-13 2000-02-24 Syngenta Limited Gene switch
WO2001002593A2 (en) * 1999-07-01 2001-01-11 Calgene Llc Control of gene expression in eukaryotic cells

Non-Patent Citations (12)

* Cited by examiner, † Cited by third party
Title
CHOI S.H. AND GREENBERG E.P.: "Genetic dissection of DNA binding and luminescence gene activation by the Vibrio fischeri LuxR protein", JOURNAL OF BACTERIOLOGY, vol. 174, no. 12, June 1992 (1992-06-01), pages 4064 - 4069, XP001002993 *
CHOI S.H. AND GREENBERG E.P.: "The C-terminal region of the Vibrio fischeri LuxR protein contains an inducer-independent lux gene activating domain", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 88, December 1991 (1991-12-01), pages 11115 - 11119, XP000876990, ISSN: 0027-8424 *
DEVINE J.H. ET AL.: "Nucleotide sequence of the luxR and luxI genes and structure of the primary regulatory region of the lux regulon of Vibrio fischeri ATCC 7744", BIOCHEMISTRY, vol. 27, 1988, pages 837 - 842, XP002152246, ISSN: 0006-2960 *
EBERHARD A. ET AL.: "Analogs of the autoinducer of bioluminescence in Vibrio fischeri", ARCHIVES OF MICROBIOLOGY, vol. 146, no. 1, 1986, pages 35 - 40, XP000884126, ISSN: 0302-8933 *
FUQUA C. ET AL.: "Census and consensus in bacterial ecosystems: the LuxR-LuxI family of quorum-sensing transcriptional regulators", ANNUAL REVIEW OF MICROBIOLOGY, vol. 50, 1996, pages 727 - 751, XP000857492, ISSN: 0066-4227 *
FUQUA W.C. AND WINANS S.C.: "A LuxR-LuxI type regulatory system activates Agrobacterium Ti plasmid conjugal transfer in the presence of a plant tumor metabolite", JOURNAL OF BACTERIOLOGY, vol. 176, no. 10, May 1994 (1994-05-01), pages 2796 - 2806, XP001010680 *
GYURIS J. ET AL.: "Cdi1, a human G1 and S phase protein phosphatase that associates with Cdk2", CELL, vol. 75, 19 November 1993 (1993-11-19), pages 791 - 803, XP000673597, ISSN: 0092-8674 *
HWANG I. ET AL.: "Modulating quorum sensing by antiactivation: TraM interacts with TraR ti inhibit activation of Ti plasmid conjugal transfer genes", MOLECULAR MICROBIOLOGY, vol. 34, no. 2, October 1999 (1999-10-01), pages 282 - 294, XP001002985 *
KAPLAN H.B. AND GREENBERG E.P.: "Overproduction and purification of the luxR gene product: transcriptional activator of the Vibrio fischeri luminescence system", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 84, October 1987 (1987-10-01), pages 6639 - 6643, XP002152249, ISSN: 0027-8424 *
ROBSON N.D. ET AL.: "Bacterial N-acyl-homoserine-lactone-dependent signalling and its potential biotechnological applications", TRENDS IN BIOTECHNOLOGY, vol. 15, no. 11, 1 November 1997 (1997-11-01), pages 458 - 464, XP004092668, ISSN: 0167-7799 *
SLOCK J. ET AL.: "Critical regions of the Vibrio fischeri LuxR protein defined by mutational analysis", JOURNAL OF BACTERIOLOGY, vol. 172, no. 7, July 1990 (1990-07-01), pages 3974 - 3979, XP000884181, ISSN: 0021-9193 *
ZHU J. AND WINANS S.C.: "Autoinducer binding by the quorum-sensing regulator TraR increases affinity for target promoters in vitro and decreases TraR turnover rates in whole cells", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE USA, vol. 96, April 1999 (1999-04-01), pages 4832 - 4837, XP002173293 *

Also Published As

Publication number Publication date
WO2001036460A2 (en) 2001-05-25
EP1232271A2 (en) 2002-08-21
AU1723001A (en) 2001-05-30
GB9927191D0 (en) 2000-01-12
CA2391971A1 (en) 2001-05-25

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