WO2001040455A3 - Compositions and methods for increasing packaging and yields of recombinant adenoviruses using multiple packaging signals - Google Patents

Compositions and methods for increasing packaging and yields of recombinant adenoviruses using multiple packaging signals Download PDF

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Publication number
WO2001040455A3
WO2001040455A3 PCT/US2000/032235 US0032235W WO0140455A3 WO 2001040455 A3 WO2001040455 A3 WO 2001040455A3 US 0032235 W US0032235 W US 0032235W WO 0140455 A3 WO0140455 A3 WO 0140455A3
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WO
WIPO (PCT)
Prior art keywords
packaging
methods
yields
compositions
recombinant adenoviruses
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2000/032235
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French (fr)
Other versions
WO2001040455A2 (en
Inventor
Guangping Gao
James M Wilson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Pennsylvania Penn
Original Assignee
University of Pennsylvania Penn
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Pennsylvania Penn filed Critical University of Pennsylvania Penn
Priority to JP2001542523A priority Critical patent/JP2003523180A/en
Priority to EP00980747A priority patent/EP1234048A2/en
Priority to AU17968/01A priority patent/AU1796801A/en
Priority to CA002392537A priority patent/CA2392537A1/en
Priority to US10/169,544 priority patent/US6821512B1/en
Publication of WO2001040455A2 publication Critical patent/WO2001040455A2/en
Publication of WO2001040455A3 publication Critical patent/WO2001040455A3/en
Anticipated expiration legal-status Critical
Priority to US10/996,341 priority patent/US20070134203A1/en
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

A recombinant adenoviral vector which has multiple adenovirus packaging domains is provided. This vector has advantages over conventional adenoviral vectors in packaging plasmid vectors into adenoviral capsids. Methods of making and using this vector are described.
PCT/US2000/032235 1999-12-03 2000-11-27 Compositions and methods for increasing packaging and yields of recombinant adenoviruses using multiple packaging signals Ceased WO2001040455A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
JP2001542523A JP2003523180A (en) 1999-12-03 2000-11-27 Compositions and methods for increasing the packaging and yield of recombinant adenovirus using multiple packaging signals
EP00980747A EP1234048A2 (en) 1999-12-03 2000-11-27 Compositions and methods for increasing packaging and yields of recombinant adenoviruses using multiple packaging signals
AU17968/01A AU1796801A (en) 1999-12-03 2000-11-27 Compositions and methods for increasing packaging and yields of recombinant adenoviruses using multiple packaging signals
CA002392537A CA2392537A1 (en) 1999-12-03 2000-11-27 Compositions and methods for increasing packaging and yields of recombinant adenoviruses using multiple packaging signals
US10/169,544 US6821512B1 (en) 1999-12-03 2000-11-27 Compositions and methods for increasing packaging and yield of recombinant adenoviruses using multiple packaging signals
US10/996,341 US20070134203A1 (en) 1999-12-03 2004-11-23 Compositions and methods for increasing packaging and yields of recombinant adenoviruses using multiple packaging signals

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US16902599P 1999-12-03 1999-12-03
US60/169,025 1999-12-03

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US10/996,341 Continuation US20070134203A1 (en) 1999-12-03 2004-11-23 Compositions and methods for increasing packaging and yields of recombinant adenoviruses using multiple packaging signals

Publications (2)

Publication Number Publication Date
WO2001040455A2 WO2001040455A2 (en) 2001-06-07
WO2001040455A3 true WO2001040455A3 (en) 2002-01-17

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/032235 Ceased WO2001040455A2 (en) 1999-12-03 2000-11-27 Compositions and methods for increasing packaging and yields of recombinant adenoviruses using multiple packaging signals

Country Status (5)

Country Link
EP (1) EP1234048A2 (en)
JP (1) JP2003523180A (en)
AU (1) AU1796801A (en)
CA (1) CA2392537A1 (en)
WO (1) WO2001040455A2 (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6989264B2 (en) 1997-09-05 2006-01-24 Targeted Genetics Corporation Methods for generating high titer helper-free preparations of released recombinant AAV vectors
US7790449B2 (en) 2001-12-17 2010-09-07 The Trustees Of The University Of Pennsylvania Adeno-associated virus (AAV) serotype 8 sequences, vectors containing the same, and uses therefor
US8524446B2 (en) 2001-11-13 2013-09-03 The Trustees Of The University Of Pennsylvania Method for detecting adeno-associated virus
US9567607B2 (en) 1998-11-05 2017-02-14 Trustees Of The University Of Pennsylvania Adeno-associated virus serotype I nucleic acid sequences, vectors and host cells containing same
US9890365B2 (en) 2014-03-09 2018-02-13 The Trustees Of The University Of Pennsylvania Compositions useful in treatment of ornithine transcarbamylase (OTC) deficiency

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE410680T1 (en) 2001-05-26 2008-10-15 One Cell Systems Inc SECRETION OF PROTEINS BY ENCAPSULATED CELLS
AU2002331669A1 (en) 2001-08-23 2003-03-10 The Wistar Institute Of Anatomy And Biology An organotypic intestinal culture and methods of use thereof
DK2573170T3 (en) 2001-12-17 2018-04-09 Univ Pennsylvania Sequences of adeno-associated virus (AAV) serotype 9, vectors containing them, and their use

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999053085A2 (en) * 1998-04-15 1999-10-21 The Research Foundation Of State University Of New York Selective regulation of adenovirus production

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999053085A2 (en) * 1998-04-15 1999-10-21 The Research Foundation Of State University Of New York Selective regulation of adenovirus production

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
GRABLE M ET AL: "CIS AND TRANS REQUIREMENTS FOR THE SELECTIVE PACKAGING OF ADENOVIRUS TYPE 5 DNA", JOURNAL OF VIROLOGY,US,NEW YORK, US, vol. 66, no. 2, 1 February 1992 (1992-02-01), pages 723 - 731, XP002012398, ISSN: 0022-538X *
HEHIR K M ET AL: "MOLECULAR CHARACTERIZATION OF REPLICATION-COMPETENT VARIANTS OF ADENOVIRUS VECTORS AND GENOME MODIFICATIONS TO PREVENT THEIR OCCURRENCE", JOURNAL OF VIROLOGY,US,THE AMERICAN SOCIETY FOR MICROBIOLOGY, vol. 70, no. 12, December 1996 (1996-12-01), pages 8459 - 8467, XP002911790, ISSN: 0022-538X *
KOCHANEK S ET AL: "A NEW ADENOVIRAL VECTOR: REPLACEMENT OF ALL VIRAL CODING SEQUENCES WITH 28 KB OF DNA INDEPENDENTLY EXPRESSING BOTH FULL-LENGTH DYSTROPHIN AND BETA-GALACTOSIDASE", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA,US,NATIONAL ACADEMY OF SCIENCE. WASHINGTON, vol. 93, 1 June 1996 (1996-06-01), pages 5731 - 5736, XP000652052, ISSN: 0027-8424 *
LANUTI, M. ET AL.: "Evaluation of an E1E4-delted adenovirus expressing the Herpes simplex thymidine kinase suicide gene in cancer gene therapy", HUMAN GENE THERAPY, vol. 10, 10 February 1999 (1999-02-10), pages 463 - 475, XP000995599 *
SANDIG, V. ET AL.: "Optimization of the helper-dependent adenovirus system for production and potency in vivo", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 97, no. 3, 1 February 2000 (2000-02-01), pages 1002 - 1007, XP000985990 *
SCHMID S I AND HEARING P: "Bipartite structure and functional independence of adenovirus 5 packaging elements", JOURNAL OF VIROLOGY,US,THE AMERICAN SOCIETY FOR MICROBIOLOGY, vol. 71, no. 5, May 1997 (1997-05-01), pages 3375 - 3384, XP002108134, ISSN: 0022-538X *
SCHMID S I ET AL: "Cellular components interact with adenovirus type 5 minimal DNA packaging domains", JOURNAL OF VIROLOGY,US,THE AMERICAN SOCIETY FOR MICROBIOLOGY, vol. 72, no. 8, August 1998 (1998-08-01), pages 6339 - 6347, XP002117828, ISSN: 0022-538X *
SEGGERN VON D J ET AL: "A HELPER-INDEPENDENT ADENOVIRUS VECTOR WITH E1, E3, AND FIBER DELETED: STRUCTURE AND INFECTIVITY OF FIBERLESS PARTICLES", JOURNAL OF VIROLOGY,US,THE AMERICAN SOCIETY FOR MICROBIOLOGY, vol. 73, no. 2, 1999, pages 1601 - 1608, XP000906914, ISSN: 0022-538X *

Cited By (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6995006B2 (en) 1997-09-05 2006-02-07 Targeted Genetics Corporation Methods for generating high titer helper-free preparations of released recombinant AAV vectors
US6989264B2 (en) 1997-09-05 2006-01-24 Targeted Genetics Corporation Methods for generating high titer helper-free preparations of released recombinant AAV vectors
US9567607B2 (en) 1998-11-05 2017-02-14 Trustees Of The University Of Pennsylvania Adeno-associated virus serotype I nucleic acid sequences, vectors and host cells containing same
US9790472B2 (en) 2001-11-13 2017-10-17 The Trustees Of The University Of Pennsylvania Method of detecting and/or identifying adeno-associated virus (AAV) sequences and isolating novel sequences identified thereby
US8524446B2 (en) 2001-11-13 2013-09-03 The Trustees Of The University Of Pennsylvania Method for detecting adeno-associated virus
US8906675B2 (en) 2001-11-13 2014-12-09 The Trustees Of The University Of Pennsylvania Adeno-associated virus (AAV) sequences and isolating novel sequences identified thereby
US9587250B2 (en) 2001-12-17 2017-03-07 Trustees Of The University Of Pennsylvania Adeno-associated virus (AAV) serotype 8 sequences, vectors containing same, and uses therefor
US10266846B2 (en) 2001-12-17 2019-04-23 The Trustees Of The University Of Pennsylvania Adeno-associated virus (AAV) serotype 8 sequences, vectors containing same, and uses therefor
US9493788B2 (en) 2001-12-17 2016-11-15 The Trustees Of The University Of Pennsylvania Adeno-associated virus (AAV) serotype 8 sequences, vectors containing same, and uses therefor
US8962332B2 (en) 2001-12-17 2015-02-24 The Trustees Of The University Of Pennsylvania Adeno-associated virus (AAV) serotype 8 sequences, vectors containing same, and uses therefor
US8318480B2 (en) 2001-12-17 2012-11-27 The Trustees Of The University Of Pennsylvania Adeno-associated virus (AAV) serotype 8 sequences, vectors containing same, and uses therefor
US9677089B2 (en) 2001-12-17 2017-06-13 The Trustees Of The University Of Pennsylvania Adeno-associated virus (AAV) serotype 8 sequences, vectors containing same, and uses therefor
US7790449B2 (en) 2001-12-17 2010-09-07 The Trustees Of The University Of Pennsylvania Adeno-associated virus (AAV) serotype 8 sequences, vectors containing the same, and uses therefor
US11396663B2 (en) 2001-12-17 2022-07-26 The Trustees Of The University Of Pennsylvania Adeno-associated virus (AAV) serotype 8 sequences, vectors containing same, and uses therefor
US11390883B2 (en) 2001-12-17 2022-07-19 The Trustees Of The University Of Pennsylvania Adeno-associated virus (AAV) serotype 8 sequences, vectors containing same, and uses therefor
US8962330B2 (en) 2001-12-17 2015-02-24 The Trustees Of The University Of Pennsylvania Adeno-associated virus (AAV) serotype 8 sequences, vectors containing same, and uses therefor
US10301650B2 (en) 2001-12-17 2019-05-28 The Trustees Of The University Of Pennsylvania Adeno-associated virus (AAV) serotype 8 sequences, vectors containing same, and uses therefor
US10590435B2 (en) 2001-12-17 2020-03-17 The Trustees Of The University Of Pennsylvania Adeno-associated virus (AAV) serotype 8 sequences, vectors containing same, and uses therefor
US10626382B2 (en) 2014-03-09 2020-04-21 The Trustees Of The University Of Pennsylvania Compositions useful in treatment of ornithine transcarbamylase (OTC) deficiency
US10781430B2 (en) 2014-03-09 2020-09-22 The Trustees Of The University Of Pennsylvania Compositions useful in treatment of ornithine transcarbamylase (OTC) deficiency
US10167454B2 (en) 2014-03-09 2019-01-01 The Trustees Of The University Of Pennsylvania Compositions useful in treatment of ornithine transcarbamylase (OTC) deficiency
US9890365B2 (en) 2014-03-09 2018-02-13 The Trustees Of The University Of Pennsylvania Compositions useful in treatment of ornithine transcarbamylase (OTC) deficiency
US11732246B2 (en) 2014-03-09 2023-08-22 The Trustees Of The University Of Pennsylvania Compositions useful in treatment of ornithine transcarbamylase (OTC) deficiency

Also Published As

Publication number Publication date
EP1234048A2 (en) 2002-08-28
CA2392537A1 (en) 2001-06-07
WO2001040455A2 (en) 2001-06-07
JP2003523180A (en) 2003-08-05
AU1796801A (en) 2001-06-12

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