WO2001044496A3 - Protease-activated receptor (par) modulator assays - Google Patents

Protease-activated receptor (par) modulator assays Download PDF

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Publication number
WO2001044496A3
WO2001044496A3 PCT/EP2000/012673 EP0012673W WO0144496A3 WO 2001044496 A3 WO2001044496 A3 WO 2001044496A3 EP 0012673 W EP0012673 W EP 0012673W WO 0144496 A3 WO0144496 A3 WO 0144496A3
Authority
WO
WIPO (PCT)
Prior art keywords
par
protease
activated receptor
assays
modulator
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2000/012673
Other languages
French (fr)
Other versions
WO2001044496A2 (en
Inventor
Ludger Altrogge
Denis Monard
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis Forschungsstiftung
Original Assignee
Novartis Forschungsstiftung
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novartis Forschungsstiftung filed Critical Novartis Forschungsstiftung
Priority to AU31585/01A priority Critical patent/AU3158501A/en
Priority to US10/149,623 priority patent/US7148018B2/en
Priority to DK00991185T priority patent/DK1238283T3/en
Priority to DE60032632T priority patent/DE60032632T2/en
Priority to EP00991185A priority patent/EP1238283B1/en
Publication of WO2001044496A2 publication Critical patent/WO2001044496A2/en
Publication of WO2001044496A3 publication Critical patent/WO2001044496A3/en
Anticipated expiration legal-status Critical
Priority to CY20071100362T priority patent/CY1106384T1/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/72—Assays involving receptors, cell surface antigens or cell surface determinants for hormones
    • G01N2333/726—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Holo Graphy (AREA)
  • Optical Communication System (AREA)
  • Gyroscopes (AREA)

Abstract

There is provided a method of identifying a PAR cleaving molecule comprising the steps of: (a) providing an immobilized PAR cleavage peptide linked to a reporter molecule; (b) contacting the immobilized PAR cleavage peptide with a PAR cleaving molecule; and (c) detecting release of the reporter molecule.
PCT/EP2000/012673 1999-12-15 2000-12-13 Protease-activated receptor (par) modulator assays Ceased WO2001044496A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
AU31585/01A AU3158501A (en) 1999-12-15 2000-12-13 Protease-activated receptor (par) modulator assays
US10/149,623 US7148018B2 (en) 1999-12-15 2000-12-13 Protease-activated receptor (par) modulator assays
DK00991185T DK1238283T3 (en) 1999-12-15 2000-12-13 Assays for modulators of protease-activated receptors (PAR)
DE60032632T DE60032632T2 (en) 1999-12-15 2000-12-13 METHOD OF IDENTIFYING MODULATORS OF PROTEASE-ACTIVATED RECEPTORS (PAR)
EP00991185A EP1238283B1 (en) 1999-12-15 2000-12-13 Protease-activated receptor (par) modulator assays
CY20071100362T CY1106384T1 (en) 1999-12-15 2007-03-15 PROTEIN-ACTIVATED RECEPTOR MODIFIER (PAR) ASSAYS

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9929674.1 1999-12-15
GBGB9929674.1A GB9929674D0 (en) 1999-12-15 1999-12-15 Protease-activated receptor (PAR) modulator assays

Publications (2)

Publication Number Publication Date
WO2001044496A2 WO2001044496A2 (en) 2001-06-21
WO2001044496A3 true WO2001044496A3 (en) 2002-05-10

Family

ID=10866389

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/012673 Ceased WO2001044496A2 (en) 1999-12-15 2000-12-13 Protease-activated receptor (par) modulator assays

Country Status (11)

Country Link
US (1) US7148018B2 (en)
EP (1) EP1238283B1 (en)
AT (1) ATE349699T1 (en)
AU (1) AU3158501A (en)
CY (1) CY1106384T1 (en)
DE (1) DE60032632T2 (en)
DK (1) DK1238283T3 (en)
ES (1) ES2277866T3 (en)
GB (1) GB9929674D0 (en)
PT (1) PT1238283E (en)
WO (1) WO2001044496A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6346510B1 (en) 1995-10-23 2002-02-12 The Children's Medical Center Corporation Therapeutic antiangiogenic endostatin compositions
US20060063930A1 (en) * 2004-08-20 2006-03-23 Agoston Gregory E Compositions and methods comprising proteinase activated receptor antagonists
JP2011530515A (en) * 2008-08-05 2011-12-22 ベーリンガー インゲルハイム インターナショナル ゲゼルシャフト ミット ベシュレンクテル ハフツング Effector of PAR-2 activation and its use in modulating inflammation
WO2013070256A2 (en) * 2011-11-07 2013-05-16 The Scripps Research Institute Protease activated receptor-1 (par1) derived cytoprotective polypeptides and related methods
US10550089B2 (en) * 2016-05-12 2020-02-04 Heptares Therapeutics Limited Inhibitors of protease-activated receptor-2

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991019000A1 (en) * 1990-06-01 1991-12-12 Gruppo Lepetit S.P.A. A MONOCLONAL ANTIBODY AGAINST THE GAG PROTEIN PRECURSOR p55 OF HUMAN IMMUNODEFICIENCY VIRUS
EP0518557A2 (en) * 1991-06-10 1992-12-16 Eli Lilly And Company Assay method for hydrolytic enzymes
WO1998018456A1 (en) * 1996-10-30 1998-05-07 The Regents Of The University Of California Protease-activated receptor 3 and uses thereof
WO1999050415A2 (en) * 1998-04-01 1999-10-07 Zymogenetics, Inc. Protease-activated receptor par4 (zchemr2)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5605809A (en) 1994-10-28 1997-02-25 Oncoimmunin, Inc. Compositions for the detection of proteases in biological samples and methods of use thereof
AU4904397A (en) 1996-10-17 1998-05-11 Chiron Corporation Protease regulator screening assay
US6243980B1 (en) 1997-03-07 2001-06-12 Tropix, Inc. Protease inhibitor assay

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991019000A1 (en) * 1990-06-01 1991-12-12 Gruppo Lepetit S.P.A. A MONOCLONAL ANTIBODY AGAINST THE GAG PROTEIN PRECURSOR p55 OF HUMAN IMMUNODEFICIENCY VIRUS
EP0518557A2 (en) * 1991-06-10 1992-12-16 Eli Lilly And Company Assay method for hydrolytic enzymes
WO1998018456A1 (en) * 1996-10-30 1998-05-07 The Regents Of The University Of California Protease-activated receptor 3 and uses thereof
WO1999050415A2 (en) * 1998-04-01 1999-10-07 Zymogenetics, Inc. Protease-activated receptor par4 (zchemr2)

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ALTROGGE LUDGER M ET AL: "An assay for high-sensitivity detection of thrombin activity and determination of proteases activating or inactivating protease-activated receptors.", ANALYTICAL BIOCHEMISTRY, vol. 277, no. 1, 1 January 2000 (2000-01-01), pages 33 - 45, XP002179398, ISSN: 0003-2697 *
CRONAN J E JR: "BIOTINATION OF PROTEINS IN-VIVO A POST-TRANSLATIONAL MODIFICATION TO LABEL PURIFY AND STUDY PROTEINS", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 265, no. 18, 1990, pages 10327 - 10333, XP002179397, ISSN: 0021-9258 *
ISHIHARA ET AL: "Protease - activated receptor 3 is a second thrombin receptor in humans", NATURE, MACMILLAN JOURNALS LTD. LONDON, GB, vol. 386, no. 6624, 3 April 1997 (1997-04-03), pages 502 - 506, XP002112285, ISSN: 0028-0836 *
KAHN M L ET AL: "GENE AND LOCUS STRUCTURE AND CHROMOSOMAL LOCALIAZATION OF THE PROTEASE-ACTIVATED RECEPTOR GENE FAMILY", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 273, no. 36, 1998, pages 23290 - 23296, XP000887171, ISSN: 0021-9258 *
VU T-K H ET AL: "MOLECULAR CLONING OF A FUNCTIONAL THROMBIN RECEPTOR REVEALS A NOVELPROTEOLYTIC MECHANISM OF RECEPTOR ACTIVATION", CELL, CELL PRESS, CAMBRIDGE, NA, US, vol. 64, 22 March 1991 (1991-03-22), pages 1057 - 1068, XP002058834, ISSN: 0092-8674 *

Also Published As

Publication number Publication date
WO2001044496A2 (en) 2001-06-21
US20030148921A1 (en) 2003-08-07
CY1106384T1 (en) 2011-10-12
AU3158501A (en) 2001-06-25
US7148018B2 (en) 2006-12-12
DE60032632T2 (en) 2007-11-15
ES2277866T3 (en) 2007-08-01
ATE349699T1 (en) 2007-01-15
DK1238283T3 (en) 2007-04-02
DE60032632D1 (en) 2007-02-08
EP1238283B1 (en) 2006-12-27
GB9929674D0 (en) 2000-02-09
PT1238283E (en) 2007-03-30
EP1238283A2 (en) 2002-09-11

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