WO2001045495A3 - Potassium channel stress-related proteins and methods of use in plants - Google Patents

Potassium channel stress-related proteins and methods of use in plants Download PDF

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Publication number
WO2001045495A3
WO2001045495A3 PCT/US2000/035356 US0035356W WO0145495A3 WO 2001045495 A3 WO2001045495 A3 WO 2001045495A3 US 0035356 W US0035356 W US 0035356W WO 0145495 A3 WO0145495 A3 WO 0145495A3
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WO
WIPO (PCT)
Prior art keywords
pcsrp
methods
plants
nucleic acid
potassium channel
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Ceased
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PCT/US2000/035356
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French (fr)
Other versions
WO2001045495A2 (en
Inventor
Manabu Ishitani
Ruoying Chen
Thielen Nocha Van
E Silva Oswaldo Da Costa
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BASF Plant Science GmbH
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BASF Plant Science GmbH
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Priority to AU29136/01A priority Critical patent/AU2913601A/en
Priority to DE60041006T priority patent/DE60041006D1/en
Priority to US10/169,038 priority patent/US7235713B2/en
Priority to EP00992189A priority patent/EP1280398B1/en
Publication of WO2001045495A2 publication Critical patent/WO2001045495A2/en
Publication of WO2001045495A3 publication Critical patent/WO2001045495A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1025Acyltransferases (2.3)
    • C12N9/1029Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

A transgenic plant transformed by a potassium channel stress related protein (PCSRP) coding nucleic acid, wherein expression of the nucleic acid sequence in the plant results in increased tolerance to environmental stress as compared to a wild type variety of the plant. Also provided are agricultural products, including seeds, produced by the transgenic plants. Also provided are isolated PCSRP, and isolated nucleic acid coding PCSRP, and vectors and host cells containing the latter. Further provided are methods of producing transgenic plants expressing PCSRP, methods of identifying novel PCSRP using antibodies, and methods of modifying the expression of PCSRP in plants.
PCT/US2000/035356 1999-12-22 2000-12-22 Potassium channel stress-related proteins and methods of use in plants Ceased WO2001045495A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU29136/01A AU2913601A (en) 1999-12-22 2000-12-22 Potassium channel stress-related proteins and methods of use in plants
DE60041006T DE60041006D1 (en) 1999-12-22 2000-12-22 STRESS-COUPLED CALIUM CHANNEL PROTEINS AND METHODS OF USE IN PLANTS
US10/169,038 US7235713B2 (en) 1999-12-22 2000-12-22 Potassium channel stress-related proteins and methods of use in plants
EP00992189A EP1280398B1 (en) 1999-12-22 2000-12-22 Stress-related potassium channel proteins and their uses in plants

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US17174599P 1999-12-22 1999-12-22
US60/171,745 1999-12-22

Publications (2)

Publication Number Publication Date
WO2001045495A2 WO2001045495A2 (en) 2001-06-28
WO2001045495A3 true WO2001045495A3 (en) 2002-01-10

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Family Applications (4)

Application Number Title Priority Date Filing Date
PCT/US2000/034972 Ceased WO2001045493A2 (en) 1999-12-22 2000-12-22 Transcription factor stress-related proteins and methods of use in plants
PCT/US2000/035100 Ceased WO2001045494A2 (en) 1999-12-22 2000-12-22 Pyrophosphatase stress-related proteins and methods of use in plants
PCT/US2000/035356 Ceased WO2001045495A2 (en) 1999-12-22 2000-12-22 Potassium channel stress-related proteins and methods of use in plants
PCT/US2000/034970 Ceased WO2001045492A2 (en) 1999-12-22 2000-12-22 Protein kinase stress-related proteins and methods of use in plants

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PCT/US2000/034972 Ceased WO2001045493A2 (en) 1999-12-22 2000-12-22 Transcription factor stress-related proteins and methods of use in plants
PCT/US2000/035100 Ceased WO2001045494A2 (en) 1999-12-22 2000-12-22 Pyrophosphatase stress-related proteins and methods of use in plants

Family Applications After (1)

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PCT/US2000/034970 Ceased WO2001045492A2 (en) 1999-12-22 2000-12-22 Protein kinase stress-related proteins and methods of use in plants

Country Status (7)

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US (7) US7164057B2 (en)
EP (5) EP1251731B1 (en)
AT (5) ATE324780T1 (en)
AU (4) AU2734001A (en)
DE (5) DE60041277D1 (en)
ES (5) ES2316400T3 (en)
WO (4) WO2001045493A2 (en)

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ATE357135T1 (en) 2007-04-15
DE60027772D1 (en) 2006-06-08
WO2001045492A3 (en) 2002-08-01
ES2279777T3 (en) 2007-09-01
US20070157343A1 (en) 2007-07-05
AU2734101A (en) 2001-07-03
DE60045115D1 (en) 2010-11-25
EP1244349A2 (en) 2002-10-02
ES2354574T3 (en) 2011-03-16
WO2001045493A2 (en) 2001-06-28
ATE418860T1 (en) 2009-01-15
AU2912301A (en) 2001-07-03
US7893322B2 (en) 2011-02-22
WO2001045495A2 (en) 2001-06-28
EP1251731A2 (en) 2002-10-30
WO2001045494A9 (en) 2002-05-30
US20090158461A1 (en) 2009-06-18
EP1797754B1 (en) 2010-10-13
ES2316400T3 (en) 2009-04-16
US20080052792A1 (en) 2008-02-28
DE60041006D1 (en) 2009-01-15
AU2734001A (en) 2001-07-03
WO2001045493A3 (en) 2002-11-07
EP1280398A2 (en) 2003-02-05
EP1251731B1 (en) 2008-12-31
ATE415812T1 (en) 2008-12-15
EP1280397A2 (en) 2003-02-05
DE60027772T2 (en) 2006-11-02
EP1244349B1 (en) 2006-05-03
EP1280398B1 (en) 2008-12-03
DE60041277D1 (en) 2009-02-12
US7902424B2 (en) 2011-03-08
US7485775B2 (en) 2009-02-03
EP1280397B1 (en) 2007-03-21
ES2258489T3 (en) 2006-09-01
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