WO2001059092A3 - Methods for generating hypermutable bacteria - Google Patents

Methods for generating hypermutable bacteria Download PDF

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Publication number
WO2001059092A3
WO2001059092A3 PCT/US2001/004339 US0104339W WO0159092A3 WO 2001059092 A3 WO2001059092 A3 WO 2001059092A3 US 0104339 W US0104339 W US 0104339W WO 0159092 A3 WO0159092 A3 WO 0159092A3
Authority
WO
WIPO (PCT)
Prior art keywords
bacteria
methods
mismatch
generating hypermutable
desirable output
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2001/004339
Other languages
French (fr)
Other versions
WO2001059092A9 (en
WO2001059092A2 (en
Inventor
Nicholas C Nicolaides
Philip M Sass
Luigi Grasso
Bert Vogelstein
Kenneth W Kinzler
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Johns Hopkins University
Original Assignee
Johns Hopkins University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Johns Hopkins University filed Critical Johns Hopkins University
Priority to AU2001234992A priority Critical patent/AU2001234992A1/en
Priority to DE60135312T priority patent/DE60135312D1/en
Priority to CA2398094A priority patent/CA2398094C/en
Priority to EP01907187A priority patent/EP1268765B1/en
Publication of WO2001059092A2 publication Critical patent/WO2001059092A2/en
Publication of WO2001059092A3 publication Critical patent/WO2001059092A3/en
Anticipated expiration legal-status Critical
Publication of WO2001059092A9 publication Critical patent/WO2001059092A9/en
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Bacteria are manipulated to create desirable output traits using dominant negative alleles of mismatch repair proteins. Enhanced hypermutation is achieved by combination of mismatch repair deficinecy and exogenously applied mutagens. Stable bacteria containing desirable output traits are obtained by restoring mismatch reparir activity to the bacteria.
PCT/US2001/004339 2000-02-11 2001-02-12 Methods for generating hypermutable bacteria Ceased WO2001059092A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU2001234992A AU2001234992A1 (en) 2000-02-11 2001-02-12 Methods for generating hypermutable bacteria
DE60135312T DE60135312D1 (en) 2000-02-11 2001-02-12 METHOD FOR THE PRODUCTION OF HYPERMUTIVE BACTERIA
CA2398094A CA2398094C (en) 2000-02-11 2001-02-12 Methods for generating hypermutable microbes
EP01907187A EP1268765B1 (en) 2000-02-11 2001-02-12 Methods for generating hypermutable bacteria

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US18192900P 2000-02-11 2000-02-11
US60/181,929 2000-02-11

Publications (3)

Publication Number Publication Date
WO2001059092A2 WO2001059092A2 (en) 2001-08-16
WO2001059092A3 true WO2001059092A3 (en) 2002-07-25
WO2001059092A9 WO2001059092A9 (en) 2002-10-24

Family

ID=22666395

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/004339 Ceased WO2001059092A2 (en) 2000-02-11 2001-02-12 Methods for generating hypermutable bacteria

Country Status (7)

Country Link
US (3) US7026119B2 (en)
EP (1) EP1268765B1 (en)
AT (1) ATE404665T1 (en)
AU (1) AU2001234992A1 (en)
CA (1) CA2398094C (en)
DE (1) DE60135312D1 (en)
WO (1) WO2001059092A2 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6365410B1 (en) * 1999-05-19 2002-04-02 Genencor International, Inc. Directed evolution of microorganisms
ATE404665T1 (en) * 2000-02-11 2008-08-15 Univ Johns Hopkins METHOD FOR PRODUCING HYPERMUTATING BACTERIA
WO2001088192A2 (en) 2000-05-12 2001-11-22 The Johns Hopkins University A method for generating hypermutable organisms
US6737268B1 (en) * 2000-11-14 2004-05-18 Morphotek, Inc. Method for generating genetically altered antigens
CA2473741C (en) * 2002-01-18 2015-12-22 Morphotek, Inc. A method for generating engineered cells for locus specific gene regulation and analysis
DE60335502D1 (en) * 2002-02-21 2011-02-03 Morphotek Inc Regulated vectors for controlling DNA hypermutability in eukaryotic cells
EP1572935A4 (en) * 2002-02-21 2006-09-13 Morphotek Inc Methods of making hypermutable cells using pmsr homologs
JP4555089B2 (en) 2002-11-15 2010-09-29 モーフオテク・インコーポレーテツド Method for producing high production amount of antibody from hybridoma created by in vitro immunization
WO2006032504A2 (en) * 2004-09-22 2006-03-30 Vrije Universiteit Brussel Method for introducing genetic mutations into plant cells
EP2115127A4 (en) * 2007-01-17 2010-04-07 Life Technologies Corp Methods and compositions for improving the health of cells in culture
JP5537420B2 (en) 2007-05-31 2014-07-02 ユニヴァーシティ オブ ワシントン Inducible mutagenesis of target genes
CN102911962B (en) * 2011-08-02 2017-03-22 中国科学院微生物研究所 Method for modifying microbes and for acquiring improved phenotype
ES2693593T3 (en) 2012-03-15 2018-12-12 Omeros Corporation Composition and method for the diversification of target sequences
US20190119667A1 (en) * 2017-09-16 2019-04-25 Massachusetts Institute Of Technology Methods for Directed Exaptation
CN108753814B (en) * 2018-06-11 2022-10-18 北京师范大学 Novel breeding method for accelerating species mutation

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6191268B1 (en) 1993-12-17 2001-02-20 Dana-Farber Cancer Institute Compositions and methods relating to DNA mismatch repair genes
US5843757A (en) 1995-08-24 1998-12-01 The Johns Hopkins University Human JTV1 gene overlaps PMS2 gene
US5907079A (en) 1996-01-18 1999-05-25 Amgen Canada Inc. MSH2 disrupted mice develop lymphomas
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
AUPO974597A0 (en) 1997-10-10 1997-11-06 Rhone-Poulenc Agro Methods for obtaining plant varieties
US6146894A (en) * 1998-04-14 2000-11-14 The Johns Hopkins University Method for generating hypermutable organisms
ATE404665T1 (en) * 2000-02-11 2008-08-15 Univ Johns Hopkins METHOD FOR PRODUCING HYPERMUTATING BACTERIA
CA2399191C (en) * 2000-02-23 2010-12-14 The Johns Hopkins University Methods for generating hypermutable yeast
US6737268B1 (en) * 2000-11-14 2004-05-18 Morphotek, Inc. Method for generating genetically altered antigens

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
ALEXANDER ARONSHTAM ET AL: "Dominant negative mutator mutations in the mutL gene of Escherichia coli", NUCLEIC ACIDS RESEARCH,OXFORD UNIVERSITY PRESS, SURREY,GB, vol. 24, no. 13, 1996, pages 2498 - 2504, XP002168605, ISSN: 0305-1048 *
BORNSCHEUER U T ET AL: "Directed evolution of an esterase for the stereoselective resolution of a key intermediate in the synthesis of epothilones", BIOTECHNOLOGY AND BIOENGINEERING - COMBINATORIAL CHEMISTRY,US,WILEY, NEW YORK, NY, vol. 58, no. 5, 5 June 1998 (1998-06-05), pages 554 - 559, XP002144278, ISSN: 0006-3592 *
FIJALKOWSKA IWONA J ET AL: "Mutants in the Exo I motif of Escherichia coli dnaQ: Defective proofreading and inviability due to error catastrophe.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 93, no. 7, 1996, 1996, pages 2856 - 2861, XP002173814, ISSN: 0027-8424 *
FISHEL R ET AL: "THE HUMAN MUTATOR GENE HOMOLOG MSH2 AND ITS ASSOCIATION WITH HEREDITARY NONPOLYPOSIS COLON CANCER", CELL,US,CELL PRESS, CAMBRIDGE, NA, vol. 75, no. 5, 3 December 1993 (1993-12-03), pages 1027 - 1038, XP000608271, ISSN: 0092-8674 *
HORST JENS-PETER ET AL: "Escherichia coli mutator genes.", TRENDS IN MICROBIOLOGY, vol. 7, no. 1, January 1999 (1999-01-01), pages 29 - 36, XP002173813, ISSN: 0966-842X *
LOH TAMALETTE ET AL: "Mutational analysis of the MutH protein from Escherichia coli.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 276, no. 15, 13 April 2001 (2001-04-13), pages 12113 - 12119, XP002173812, ISSN: 0021-9258 *
PRUDHOMME M ET AL: "MISMATCH REPAIR GENES OF STREPTOCOCCUS-PNEUMONIAE HEXA CONFERS A MUTATOR PHENOTYPE IN ESCHERICHIA-COLI BY NEGATIVE COMPLEMENTATION", JOURNAL OF BACTERIOLOGY, vol. 173, no. 22, 1991, pages 7196 - 7203, XP001013041, ISSN: 0021-9193 *
STRATAGENE: "XL1-Red Competent Cells", INTERNET, 2001, XP002173919, Retrieved from the Internet <URL:http://www.stratagene.com/manuals/200129.pdf> [retrieved on 20010802] *

Also Published As

Publication number Publication date
EP1268765A2 (en) 2003-01-02
EP1268765B1 (en) 2008-08-13
US20060194236A1 (en) 2006-08-31
US20020068284A1 (en) 2002-06-06
US7892832B2 (en) 2011-02-22
ATE404665T1 (en) 2008-08-15
CA2398094C (en) 2013-01-08
US7026119B2 (en) 2006-04-11
WO2001059092A9 (en) 2002-10-24
CA2398094A1 (en) 2001-08-16
DE60135312D1 (en) 2008-09-25
US7695969B2 (en) 2010-04-13
US20080318321A1 (en) 2008-12-25
AU2001234992A1 (en) 2001-08-20
WO2001059092A2 (en) 2001-08-16

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