WO2001061015A3 - Thermostable chimeric nucleic acid polymerases and uses thereof - Google Patents

Thermostable chimeric nucleic acid polymerases and uses thereof Download PDF

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Publication number
WO2001061015A3
WO2001061015A3 PCT/EP2001/001790 EP0101790W WO0161015A3 WO 2001061015 A3 WO2001061015 A3 WO 2001061015A3 EP 0101790 W EP0101790 W EP 0101790W WO 0161015 A3 WO0161015 A3 WO 0161015A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
chimeric nucleic
polymerases
acid polymerases
thermostable
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2001/001790
Other languages
German (de)
French (fr)
Other versions
WO2001061015A2 (en
Inventor
Dirk Loeffert
Andreas Missel
Jie Kang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qiagen GmbH
Original Assignee
Qiagen GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen GmbH filed Critical Qiagen GmbH
Priority to DE60133992T priority Critical patent/DE60133992D1/en
Priority to EP01953004A priority patent/EP1259624B1/en
Publication of WO2001061015A2 publication Critical patent/WO2001061015A2/en
Publication of WO2001061015A3 publication Critical patent/WO2001061015A3/en
Priority to US10/216,682 priority patent/US8637288B2/en
Anticipated expiration legal-status Critical
Priority to US14/108,863 priority patent/US20140154748A1/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Heat Sensitive Colour Forming Recording (AREA)

Abstract

Novel thermostable chimeric nucleic acid polymerases and methods for their generation and use are disclosed. It is shown that these chimeric nucleic acid polymerases, such as DNA polymerases, can be constructed using enzymatically active domains, isolated from different proteins or chemically synthesized. It is demonstrated that chimeric nucleic acid polymerases of the present invention possess the chemical and physical properties of their component domains (e.g., exonuclease activity, thermostability) and that the chimeric polymerases are thermostable.
PCT/EP2001/001790 2000-02-17 2001-02-16 Thermostable chimeric nucleic acid polymerases and uses thereof Ceased WO2001061015A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
DE60133992T DE60133992D1 (en) 2000-02-17 2001-02-16 THERMOSTABILE CHIMERIC NUCLEIC ACID POLYMERASES AND ITS USES
EP01953004A EP1259624B1 (en) 2000-02-17 2001-02-16 Thermostable chimeric nucleic acid polymerases and uses thereof
US10/216,682 US8637288B2 (en) 2000-02-17 2002-08-08 Thermostable chimeric nucleic acid polymerases and uses thereof
US14/108,863 US20140154748A1 (en) 2000-02-17 2013-12-17 Thermostable chimeric nucleic acid polymerases and uses thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US50615300A 2000-02-17 2000-02-17
US09/506,153 2000-02-17

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
US50615300A Continuation-In-Part 2000-02-17 2000-02-17

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US10/216,682 Continuation US8637288B2 (en) 2000-02-17 2002-08-08 Thermostable chimeric nucleic acid polymerases and uses thereof

Publications (2)

Publication Number Publication Date
WO2001061015A2 WO2001061015A2 (en) 2001-08-23
WO2001061015A3 true WO2001061015A3 (en) 2002-04-25

Family

ID=24013413

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/001790 Ceased WO2001061015A2 (en) 2000-02-17 2001-02-16 Thermostable chimeric nucleic acid polymerases and uses thereof

Country Status (5)

Country Link
US (2) US8637288B2 (en)
EP (2) EP1925669B1 (en)
AT (2) ATE491031T1 (en)
DE (2) DE60143632D1 (en)
WO (1) WO2001061015A2 (en)

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US6627424B1 (en) 2000-05-26 2003-09-30 Mj Bioworks, Inc. Nucleic acid modifying enzymes
DE10049211A1 (en) 2000-10-05 2002-04-18 Qiagen Gmbh Thermostable polymerase from Thermococcus pacificus
AU2002351193A1 (en) 2001-11-28 2003-06-10 Mj Bioworks Incorporated Parallel polymorphism scoring by amplification and error correction
JP4575160B2 (en) * 2002-07-25 2010-11-04 バイオ−ラッド ラボラトリーズ インコーポレーティッド Hybrid protein methods and compositions
US7560260B2 (en) 2002-07-25 2009-07-14 Bio-Rad Laboratories, Inc. Compositions with polymerase activity
US7960157B2 (en) 2002-12-20 2011-06-14 Agilent Technologies, Inc. DNA polymerase blends and uses thereof
JP4722035B2 (en) 2003-03-25 2011-07-13 アジレント・テクノロジーズ・インク DNA polymerase fusion and use thereof
US20060099567A1 (en) * 2004-04-08 2006-05-11 Biomatrica, Inc. Integration of sample storage and sample management for life science
US20080176209A1 (en) * 2004-04-08 2008-07-24 Biomatrica, Inc. Integration of sample storage and sample management for life science
CA2560513A1 (en) * 2004-04-08 2005-12-01 Biomatrica, Inc. Integration of sample storage and sample management for life science
GB2416538B (en) * 2004-07-26 2008-07-30 Bioline Ltd Chimeric DNA polymerase
US9481912B2 (en) * 2006-09-12 2016-11-01 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for detecting and identifying nucleic acid sequences in biological samples
US8652782B2 (en) * 2006-09-12 2014-02-18 Longhorn Vaccines & Diagnostics, Llc Compositions and methods for detecting, identifying and quantitating mycobacterial-specific nucleic acids
US11041215B2 (en) 2007-08-24 2021-06-22 Longhorn Vaccines And Diagnostics, Llc PCR ready compositions and methods for detecting and identifying nucleic acid sequences
US20140038174A1 (en) * 2011-04-26 2014-02-06 Longhorn Vaccines And Diagnostics, Llc Compositions and Methods for Detecting and Identifying Nucleic Acid Sequences in Biological Samples
DK2279263T3 (en) 2008-04-30 2013-11-11 Integrated Dna Tech Inc RNASE-H-BASED ASSAYS USING MODIFIED RNA MONOMERS
US9434988B2 (en) 2008-04-30 2016-09-06 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
US8911948B2 (en) 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
WO2010062776A2 (en) 2008-11-03 2010-06-03 Kapabiosystems Chimeric dna polymerases
EP2430195B1 (en) * 2009-05-11 2019-01-23 Biomatrica, INC. Compositions and methods for biological sample storage
US9845489B2 (en) 2010-07-26 2017-12-19 Biomatrica, Inc. Compositions for stabilizing DNA, RNA and proteins in saliva and other biological samples during shipping and storage at ambient temperatures
CA2806670A1 (en) 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures
US9725703B2 (en) 2012-12-20 2017-08-08 Biomatrica, Inc. Formulations and methods for stabilizing PCR reagents
US10023856B2 (en) * 2013-09-25 2018-07-17 Thermo Fisher Scientific Baltics Uab Enzyme composition for DNA end repair, adenylation, phosphorylation
EP2986719B1 (en) 2013-12-06 2020-07-29 Bio-Rad Laboratories, Inc. Fusion polymerases
CN106572650B (en) 2014-06-10 2021-08-31 生物马特里卡公司 Stabilizes thrombocytes at ambient temperature
US10364420B2 (en) 2015-12-07 2019-07-30 Bio-Rad Laboratories, Inc Dimeric reverse transcriptase
SG11201804776SA (en) 2015-12-08 2018-07-30 Biomatrica Inc Reduction of erythrocyte sedimentation rate
CN114829593B (en) * 2019-12-23 2023-11-14 深圳华大生命科学研究院 Chimeric DNA polymerase and application thereof
CN117050967B (en) * 2023-08-21 2024-04-09 南京诺唯赞生物科技股份有限公司 A method for improving GC balance of next-generation sequencing library

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Publication number Priority date Publication date Assignee Title
WO1992006200A1 (en) * 1990-09-28 1992-04-16 F. Hoffmann-La-Roche Ag 5' to 3' exonuclease mutations of thermostable dna polymerases
WO1997029209A1 (en) * 1996-02-09 1997-08-14 President And Fellows Of Harvard College Dna polymerase with modified processivity
EP0892058A2 (en) * 1997-07-09 1999-01-20 F. Hoffmann-La Roche Ag Mutant chimeric DNA polymerases
WO1999047649A2 (en) * 1998-03-13 1999-09-23 Roche Diagnostics Gmbh Polymerase chimeras
GB2344591A (en) * 1998-08-24 2000-06-14 Bioline Limited A chimaeric DNA polymerase
WO2001018213A1 (en) * 1999-09-09 2001-03-15 Dzieglewska, Hanna Chimeric thermostable dna polymerases

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WO2001061015A2 (en) 2000-02-17 2001-08-23 Qiagen Gmbh Thermostable chimeric nucleic acid polymerases and uses thereof
US6627424B1 (en) * 2000-05-26 2003-09-30 Mj Bioworks, Inc. Nucleic acid modifying enzymes
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Patent Citations (6)

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WO1992006200A1 (en) * 1990-09-28 1992-04-16 F. Hoffmann-La-Roche Ag 5' to 3' exonuclease mutations of thermostable dna polymerases
WO1997029209A1 (en) * 1996-02-09 1997-08-14 President And Fellows Of Harvard College Dna polymerase with modified processivity
EP0892058A2 (en) * 1997-07-09 1999-01-20 F. Hoffmann-La Roche Ag Mutant chimeric DNA polymerases
WO1999047649A2 (en) * 1998-03-13 1999-09-23 Roche Diagnostics Gmbh Polymerase chimeras
GB2344591A (en) * 1998-08-24 2000-06-14 Bioline Limited A chimaeric DNA polymerase
WO2001018213A1 (en) * 1999-09-09 2001-03-15 Dzieglewska, Hanna Chimeric thermostable dna polymerases

Also Published As

Publication number Publication date
DE60143632D1 (en) 2011-01-20
DE60133992D1 (en) 2008-06-26
US20030138805A1 (en) 2003-07-24
EP1925669A1 (en) 2008-05-28
ATE491031T1 (en) 2010-12-15
EP1259624A2 (en) 2002-11-27
EP1259624B1 (en) 2008-05-14
US20140154748A1 (en) 2014-06-05
WO2001061015A2 (en) 2001-08-23
US8637288B2 (en) 2014-01-28
EP1925669B1 (en) 2010-12-08
ATE395420T1 (en) 2008-05-15

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