WO2001068831A3 - Effective nuclear reprogramming in mammals - Google Patents

Effective nuclear reprogramming in mammals Download PDF

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Publication number
WO2001068831A3
WO2001068831A3 PCT/US2001/008371 US0108371W WO0168831A3 WO 2001068831 A3 WO2001068831 A3 WO 2001068831A3 US 0108371 W US0108371 W US 0108371W WO 0168831 A3 WO0168831 A3 WO 0168831A3
Authority
WO
WIPO (PCT)
Prior art keywords
oocyte
genetic material
mammals
introducing
donor genetic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2001/008371
Other languages
French (fr)
Other versions
WO2001068831A2 (en
Inventor
Steven Stice
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Georgia Research Foundation Inc
Original Assignee
University of Georgia Research Foundation Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Georgia Research Foundation Inc filed Critical University of Georgia Research Foundation Inc
Priority to AT01918733T priority Critical patent/ATE517990T1/en
Priority to EP01918733A priority patent/EP1356031B1/en
Priority to AU2001245776A priority patent/AU2001245776A1/en
Priority to CA2403344A priority patent/CA2403344C/en
Priority to DK01918733.5T priority patent/DK1356031T3/en
Publication of WO2001068831A2 publication Critical patent/WO2001068831A2/en
Anticipated expiration legal-status Critical
Publication of WO2001068831A3 publication Critical patent/WO2001068831A3/en
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Casting Devices For Molds (AREA)
  • Molds, Cores, And Manufacturing Methods Thereof (AREA)
  • Stored Programmes (AREA)

Abstract

The present invention provides methods of producing a cloned non-human mammalian nuclear transfer (NT) embryo and methods for producing a cloned non-human mammal. Embodiments of the methods include introducing donor genetic material into a metaphase I oocyte; introducing donor genetic material into a non-enucleated oocyte; introducing donor genetic material obtained from a donor cell that is at metaphase into an oocyte; introducing donor genetic material into an oocyte, and naturally activating the oocyte or the NT embryo; and introducing donor genetic material obtained from a donor cell that is at late G1 phase into an oocyte.
PCT/US2001/008371 2000-03-15 2001-03-15 Effective nuclear reprogramming in mammals Ceased WO2001068831A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AT01918733T ATE517990T1 (en) 2000-03-15 2001-03-15 EFFECTIVE NUCLEAR REPROGRAMMING IN MAMMALS
EP01918733A EP1356031B1 (en) 2000-03-15 2001-03-15 Effective nuclear reprogramming in mammals
AU2001245776A AU2001245776A1 (en) 2000-03-15 2001-03-15 Effective nuclear reprogramming in mammals
CA2403344A CA2403344C (en) 2000-03-15 2001-03-15 Effective nuclear reprogramming in mammals
DK01918733.5T DK1356031T3 (en) 2000-03-15 2001-03-15 Efficient core reprogramming in mammals

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US18968600P 2000-03-15 2000-03-15
US60/189,686 2000-03-15

Publications (2)

Publication Number Publication Date
WO2001068831A2 WO2001068831A2 (en) 2001-09-20
WO2001068831A3 true WO2001068831A3 (en) 2003-08-28

Family

ID=22698365

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/008371 Ceased WO2001068831A2 (en) 2000-03-15 2001-03-15 Effective nuclear reprogramming in mammals

Country Status (7)

Country Link
US (2) US6906238B2 (en)
EP (1) EP1356031B1 (en)
AT (1) ATE517990T1 (en)
AU (1) AU2001245776A1 (en)
CA (1) CA2403344C (en)
DK (1) DK1356031T3 (en)
WO (1) WO2001068831A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE360361T1 (en) * 2000-09-28 2007-05-15 Sangamo Biosciences Inc NUCLEAR REPROGRAMMING IN ISWI AND SIMILAR CHROMATIN REMODELING ATPASE
US7491534B2 (en) 2000-12-22 2009-02-17 Kirin Holdings Kabushiki Kaisha Methods for altering cell fate to generate T-cells specific for an antigen of interest
US20020142397A1 (en) 2000-12-22 2002-10-03 Philippe Collas Methods for altering cell fate
ATE502106T1 (en) * 2000-12-22 2011-04-15 Kyowa Hakko Kirin Co Ltd METHOD FOR CLONING NON-HUMAN MAMMALS USING REPROGRAMMED DONOR CHROMATIN OR DONOR CELLS
CN1379977A (en) * 2001-04-10 2002-11-20 陆长富 Method for constituting clonal embryo of mammal and human clonal embryo constitution
JP3842597B2 (en) * 2001-08-14 2006-11-08 家畜受精卵移植技術研究組合 G1 phase cell collection method and nuclear transfer method using the cell
US20080222745A1 (en) * 2007-03-07 2008-09-11 Utah State University Colcemid-Treatment of Oocytes to enhance Nuclear Transfer Cloning
JP2010517563A (en) * 2007-11-19 2010-05-27 ソウル ナショナル ユニバーシティ インダストリー ファウンデーション Method for improving production efficiency of somatic cell nuclear transfer offspring in canines
EP2232985A4 (en) * 2008-01-04 2011-02-16 Seoul Nat Univ Ind Foundation PROCESS FOR PRODUCING CLONED TRANSGENIC CANNONES

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998030683A2 (en) * 1997-01-10 1998-07-16 University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts Nuclear transfer with differentiated fetal and adult donor cells

Family Cites Families (19)

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Publication number Priority date Publication date Assignee Title
US4994384A (en) 1986-12-31 1991-02-19 W. R. Grace & Co.-Conn. Multiplying bovine embryos
US5057420A (en) 1987-06-05 1991-10-15 Granada Biosciences, Inc. Bovine nuclear transplantation
US5213979A (en) 1987-12-30 1993-05-25 W. R. Grace & Co.-Conn. In vitro culture of bovine embryos
IL94184A0 (en) 1989-05-01 1991-01-31 Granada Biosciences Inc In vitro maturation of bovine oocytes
US5160312A (en) 1990-02-09 1992-11-03 W. R. Grace & Co.-Conn. Cryopreservation process for direct transfer of embryos
CA2086330A1 (en) 1990-07-09 1992-01-10 Richard C. Fry Enhanced implantation, development and maintenance of embryos using leukaemia inhibitory factor
US5096822A (en) 1990-07-26 1992-03-17 W. R. Grace & Co.- Conn. Bovine embryo medium
US5453366A (en) 1990-07-26 1995-09-26 Sims; Michele M. Method of cloning bovine embryos
CA2072475A1 (en) 1991-07-01 1993-01-02 Tanja Dominko In vitro maturation of denuded bovine oocytes
US5480772A (en) 1993-02-03 1996-01-02 Brandeis University In vitro activation of a nucleus
US5496720A (en) 1993-02-10 1996-03-05 Susko-Parrish; Joan L. Parthenogenic oocyte activation
WO1995016770A1 (en) 1993-12-17 1995-06-22 Abs Global, Inc. Ungulate preblastocyst derived embryonic stem cells and use thereof to produce cloned transgenic and chimeric ungulates
GB9417831D0 (en) 1994-09-05 1994-10-26 Biotech & Biolog Scien Res Biological manipulation
GB9517779D0 (en) 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
GB9517780D0 (en) 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
BR9711204A (en) 1996-08-19 1999-08-17 Univ Massachusetts Embryonic cell lines or similar are undifferentiated produced by nuclear transplantation of hybrid species
US6235969B1 (en) * 1997-01-10 2001-05-22 University Of Massachusetts Cloning pigs using donor nuclei from non-quiescent differentiated cells
NZ337495A (en) 1997-03-06 2001-06-29 Infigen Inc Bovine primordial germ cells and their use in cloning
GB2340943B (en) 1998-08-28 2000-07-19 Bank Of England Improvements in and relating to sheet material inspection apparatus and methods

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998030683A2 (en) * 1997-01-10 1998-07-16 University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts Nuclear transfer with differentiated fetal and adult donor cells

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
CHASTANT-MAILLARD S. ET AL.: "Clonage et reprogrammation du noyau dans l'embryon préimplantatoire", GYNECOL OBSTET FERTIL., 28 September 2002 (2002-09-28), pages 649 - 658, XP008004095 *
KEEFER C L ET AL: "EFFECT OF FOLLICLE-STIMULATING HORMONE AND LUTEINIZING HORMONE DURING BOVINE IN VITRO MATURATION ON DEVELOPMENT FOLLOWING IN VITROFERTILIZATION AND NUCLEAR TRANSFER", MOLECULAR REPRODUCTION AND DEVELOPMENT, LISSS, NEW YORK, NY, US, vol. 36, 1993, pages 469 - 474, XP002937732, ISSN: 1040-452X *
KIKYO NOBUAKI ET AL: "Reprogramming nuclei: Insights from cloning, nuclear transfer and heterokaryons", JOURNAL OF CELL SCIENCE, ESSEX, GB, vol. 113, no. 1, January 2000 (2000-01-01), pages 11 - 20, XP002176693 *
MIYOSHI, K. ET AL.: "Development of porcine embryos reconstituted with somatic cells and enucleated metaphase I and II oocytes matures in a protein-free medium", DEVELOPMENTAL BIOLOGY, vol. 1, 11 July 2001 (2001-07-11), pages 12, XP002201739 *
POLEJAEVA I A ET AL: "CLONED PIGS PRODUCED BY NUCLEAR TRANSFER FROM ADULT SOMATIC CELLS", NATURE, MACMILLAN JOURNALS LTD. LONDON, GB, vol. 407, 7 September 2000 (2000-09-07), pages 86 - 90, XP000986094, ISSN: 0028-0836 *
POLEJAEVA I A ET AL: "NEW ADVANCES IN SOMATIC CELL NUCLEAR TRANSFER: APPLICATION IN TRANSGENESIS", THERIOGENOLOGY, LOS ALTOS, CA, US, vol. 53, no. 1, 1 January 2000 (2000-01-01), pages 117 - 126, XP000940942, ISSN: 0093-691X *
STICE S L ET AL: "CLONING: NEW BREAKTHROUGHS LEADING TO COMMERCIAL OPPORTUNITIES", THERIOGENOLOGY, LOS ALTOS, CA, US, vol. 49, 1998, pages 129 - 138, XP002935721, ISSN: 0093-691X *

Also Published As

Publication number Publication date
DK1356031T3 (en) 2011-10-31
US20010053550A1 (en) 2001-12-20
US7547818B2 (en) 2009-06-16
US6906238B2 (en) 2005-06-14
US20040187173A1 (en) 2004-09-23
CA2403344C (en) 2013-05-21
AU2001245776A1 (en) 2001-09-24
ATE517990T1 (en) 2011-08-15
CA2403344A1 (en) 2001-09-20
WO2001068831A2 (en) 2001-09-20
EP1356031A2 (en) 2003-10-29
EP1356031B1 (en) 2011-07-27

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