WO2001070816A3 - Ecdysone receptor-based inducible gene expression system - Google Patents

Ecdysone receptor-based inducible gene expression system Download PDF

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Publication number
WO2001070816A3
WO2001070816A3 PCT/US2001/009050 US0109050W WO0170816A3 WO 2001070816 A3 WO2001070816 A3 WO 2001070816A3 US 0109050 W US0109050 W US 0109050W WO 0170816 A3 WO0170816 A3 WO 0170816A3
Authority
WO
WIPO (PCT)
Prior art keywords
gene expression
expression system
inducible gene
relates
ecdysone receptor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2001/009050
Other languages
French (fr)
Other versions
WO2001070816A2 (en
Inventor
Subba Reddy Palli
Marianna Zinovjevna Kapitskaya
Dean Ervin Cress
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rohm and Haas Co
Original Assignee
Rohm and Haas Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to DE60122685T priority Critical patent/DE60122685T2/en
Priority to AU2001249315A priority patent/AU2001249315A1/en
Priority to US10/239,134 priority patent/US20040033600A1/en
Priority to EP01922524A priority patent/EP1266015B1/en
Priority to MXPA02009157A priority patent/MXPA02009157A/en
Priority to JP2001569016A priority patent/JP5031967B2/en
Priority to CA2404253A priority patent/CA2404253C/en
Application filed by Rohm and Haas Co filed Critical Rohm and Haas Co
Priority to ARP010101339A priority patent/AR028276A1/en
Priority to US09/965,703 priority patent/US7091038B2/en
Publication of WO2001070816A2 publication Critical patent/WO2001070816A2/en
Publication of WO2001070816A3 publication Critical patent/WO2001070816A3/en
Anticipated expiration legal-status Critical
Priority to US11/677,968 priority patent/US7807417B2/en
Priority to AU2007200882A priority patent/AU2007200882B2/en
Priority to US11/841,495 priority patent/US7776587B2/en
Priority to US12/818,034 priority patent/US8202718B2/en
Priority to US12/859,940 priority patent/US20110059530A1/en
Priority to US13/463,527 priority patent/US8822754B2/en
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70567—Nuclear receptors, e.g. retinoic acid receptor [RAR], RXR, nuclear orphan receptors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
    • C07K14/721—Steroid/thyroid hormone superfamily, e.g. GR, EcR, androgen receptor, oestrogen receptor
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • High Energy & Nuclear Physics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Microbiology (AREA)
  • Endocrinology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

This invention relates to the field of biotechnology or genetic engineering. Specifically, this invention relates to the field of gene expression. More specifically, this invention relates to a novel inducible gene expression system and methods of modulating gene expression in a host cell for applications such as gene therapy, large scale production of proteins and antibodies, cell-based high throughput screening assays, functional genomics and regulation of traits in transgenic plants and animals.
PCT/US2001/009050 2000-03-22 2001-03-21 Ecdysone receptor-based inducible gene expression system Ceased WO2001070816A2 (en)

Priority Applications (15)

Application Number Priority Date Filing Date Title
AU2001249315A AU2001249315A1 (en) 2000-03-22 2001-03-21 Ecdysone receptor-based inducible gene expression system
US10/239,134 US20040033600A1 (en) 2001-03-21 2001-03-21 Ecdysone receptor-based inducible gene expression system
EP01922524A EP1266015B1 (en) 2000-03-22 2001-03-21 Ecdysone receptor-based inducible gene expression system
MXPA02009157A MXPA02009157A (en) 2000-03-22 2001-03-21 Novel ecdysone receptor-based inducible gene expression system.
JP2001569016A JP5031967B2 (en) 2000-03-22 2001-03-21 A novel ecdysone receptor-based inducible gene expression system
DE60122685T DE60122685T2 (en) 2000-03-22 2001-03-21 INDUCED GENE EXPRESSION SYSTEM BASED ON ECDYSON RECEPTORS
CA2404253A CA2404253C (en) 2000-03-22 2001-03-21 Novel ecdysone receptor-based inducible gene expression system
ARP010101339A AR028276A1 (en) 2000-03-22 2001-03-22 NEW INDUCIBLE GCNICA EXPRESSION SYSTEM BASED ON ECDISONA RECEIVER
US09/965,703 US7091038B2 (en) 2000-03-22 2001-09-26 Ecdysone receptor-based inducible gene expression system
US11/677,968 US7807417B2 (en) 2000-03-22 2007-02-22 Ecdysone receptor-based inducible gene expression system
AU2007200882A AU2007200882B2 (en) 2000-03-22 2007-02-28 Ecdysone receptor-based inducible gene expression system
US11/841,495 US7776587B2 (en) 2000-03-22 2007-08-20 Ecdysone receptor-based inducible gene expression system
US12/818,034 US8202718B2 (en) 2000-03-22 2010-06-17 Ecdysone receptor-based inducible gene expression system
US12/859,940 US20110059530A1 (en) 2000-03-22 2010-08-20 Novel Ecdysone Receptor-Based Inducible Gene Expression System
US13/463,527 US8822754B2 (en) 2000-03-22 2012-05-03 Ecdysone receptor-based inducible gene expression system

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US19135500P 2000-03-22 2000-03-22
US60/191,355 2000-03-22
US26979901P 2001-02-20 2001-02-20
US60/269,799 2001-02-20

Related Child Applications (3)

Application Number Title Priority Date Filing Date
US10239134 A-371-Of-International 2001-03-21
US09/965,703 Continuation-In-Part US7091038B2 (en) 2000-03-22 2001-09-26 Ecdysone receptor-based inducible gene expression system
US11/677,968 Continuation US7807417B2 (en) 2000-03-22 2007-02-22 Ecdysone receptor-based inducible gene expression system

Publications (2)

Publication Number Publication Date
WO2001070816A2 WO2001070816A2 (en) 2001-09-27
WO2001070816A3 true WO2001070816A3 (en) 2002-08-29

Family

ID=26886975

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/009050 Ceased WO2001070816A2 (en) 2000-03-22 2001-03-21 Ecdysone receptor-based inducible gene expression system

Country Status (11)

Country Link
US (1) US7091038B2 (en)
EP (1) EP1266015B1 (en)
JP (1) JP5031967B2 (en)
CN (1) CN1304578C (en)
AR (1) AR028276A1 (en)
AT (1) ATE338135T1 (en)
AU (2) AU2001249315A1 (en)
CA (1) CA2404253C (en)
DE (1) DE60122685T2 (en)
MX (1) MXPA02009157A (en)
WO (1) WO2001070816A2 (en)

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US7563928B2 (en) 2003-02-28 2009-07-21 Intrexon Corporation Bioavailable diacylhydrazine ligands for modulating the expression of exogenous genes via an ecdysone receptor complex
US11555197B2 (en) 2016-07-25 2023-01-17 Greenvenus, Llc Control of phenotype in plants
US12612647B2 (en) 2017-02-28 2026-04-28 The Trustees Of The University Of Pennsylvania Compositions useful in treatment of spinal muscular atrophy

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US8105825B2 (en) * 2000-10-03 2012-01-31 Intrexon Corporation Multiple inducible gene regulation system
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EP1373470B1 (en) 2001-02-20 2013-04-24 Intrexon Corporation Novel substitution mutant receptors and their use in a nuclear receptor-based inducible gene expression system
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WO2002066614A2 (en) * 2001-02-20 2002-08-29 Rheogene Holdings, Inc. Chimeric retinoid x receptors and their use in a novel ecdysone receptor-based inducible gene expression system
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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996027673A1 (en) * 1995-03-03 1996-09-12 Novartis Ag Control of gene expression in plants by receptor mediated transactivation in the presence of a chemical ligand
EP0798378A2 (en) * 1996-03-26 1997-10-01 Akzo Nobel N.V. Estrogen receptor
WO1999002683A1 (en) * 1997-07-10 1999-01-21 The Salk Institute For Biological Studies Modified lepidopteran receptors and hybrid multifunctional proteins for use in regulation of transgene expression
WO1999010510A2 (en) * 1997-08-26 1999-03-04 Ariad Gene Therapeutics, Inc. Fusion proteins comprising a dimerization, trimerization or tetramerization domain and an additional heterologous transcription activation, transcription repression, dna binding or ligand binding domain
WO1999058155A1 (en) * 1998-05-14 1999-11-18 The Salk Institute For Biological Studies Formulations useful for modulating expression of exogenous genes in mammalian systems, and products related thereto
EP0965644A2 (en) * 1998-06-17 1999-12-22 Rohm And Haas Company Ligands for modulating the expression of exogenous genes via an ecdysone receptor complex

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5283173A (en) 1990-01-24 1994-02-01 The Research Foundation Of State University Of New York System to detect protein-protein interactions
JPH11508121A (en) 1990-03-22 1999-07-21 ザ ソーク インスティテュート フォア バイオロジカル スタディーズ Insect retinoid receptor compositions and methods
AU734051B2 (en) * 1996-04-05 2001-05-31 Salk Institute For Biological Studies, The Hormone-mediated methods for modulating expression of exogenous genes in mammalian systems, and products related thereto
US5919667A (en) * 1996-06-20 1999-07-06 The Salk Institute For Biological Studies Modular assembly retroviral vectors and uses thereof
US6300488B1 (en) 1997-07-10 2001-10-09 The Salk Institute For Biological Studies Modified lepidopteran receptors and hybrid multifunctional proteins for use in transcription and regulation of transgene expression

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996027673A1 (en) * 1995-03-03 1996-09-12 Novartis Ag Control of gene expression in plants by receptor mediated transactivation in the presence of a chemical ligand
EP0798378A2 (en) * 1996-03-26 1997-10-01 Akzo Nobel N.V. Estrogen receptor
WO1999002683A1 (en) * 1997-07-10 1999-01-21 The Salk Institute For Biological Studies Modified lepidopteran receptors and hybrid multifunctional proteins for use in regulation of transgene expression
WO1999010510A2 (en) * 1997-08-26 1999-03-04 Ariad Gene Therapeutics, Inc. Fusion proteins comprising a dimerization, trimerization or tetramerization domain and an additional heterologous transcription activation, transcription repression, dna binding or ligand binding domain
WO1999058155A1 (en) * 1998-05-14 1999-11-18 The Salk Institute For Biological Studies Formulations useful for modulating expression of exogenous genes in mammalian systems, and products related thereto
EP0965644A2 (en) * 1998-06-17 1999-12-22 Rohm And Haas Company Ligands for modulating the expression of exogenous genes via an ecdysone receptor complex

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
CHRISTOPHERSON K S ET AL: "ECDYSTEROID-DEPENDENT REGULATION OF GENES IN MAMMALIAN CELLS BY A DROSOPHILA ECDYSONE RECEPTOR AND CHIMERIC TRANSACTIVATORS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 89, July 1992 (1992-07-01), pages 6314 - 6318, XP002921831, ISSN: 0027-8424 *
KAKIZAWA TOMOKO ET AL: "Ligand-dependent heterodimerization of thyroid hormone receptor and retinoid X receptor.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 272, no. 38, 1997, pages 23799 - 23804, XP002200320, ISSN: 0021-9258 *
KOELLE M R ET AL: "THE DROSOPHILA ECR GENE ENCODES AN ECDYSONE RECEPTOR, A NEW MEMBER OF THE STEROID RECEPTOR SUPERFAMILY", CELL, CELL PRESS, CAMBRIDGE, NA, US, vol. 67, no. 1, 4 October 1991 (1991-10-04), pages 59 - 77, XP001010154, ISSN: 0092-8674 *
LEID M ET AL: "PURIFICATION, CLONING, AND RXR IDENTITY OF THE HELA CELL FACTOR WITH WHICH RAR OR TR HETERODIMERIZES TO BIND TARGET SEQUENCES EFFICIENTLY", CELL, CELL PRESS, CAMBRIDGE, NA, US, vol. 68, 24 January 1992 (1992-01-24), pages 377 - 395, XP002007203, ISSN: 0092-8674 *
PERERA SRINI C ET AL: "An analysis of ecdysone receptor domains required for heterodimerization with ultraspiracle.", ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY, vol. 41, no. 2, 1999, pages 61 - 70, XP001077866, ISSN: 0739-4462 *
PERERA SRINI C ET AL: "Studies on two ecdysone receptor isoforms of the spruce budworm, Choristoneura fumiferana.", MOLECULAR AND CELLULAR ENDOCRINOLOGY, vol. 152, no. 1-2, 25 June 1999 (1999-06-25), pages 73 - 84, XP002200321, ISSN: 0303-7207 *

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