WO2001081375A3 - Dna & protein binding miniature proteins - Google Patents

Dna & protein binding miniature proteins Download PDF

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Publication number
WO2001081375A3
WO2001081375A3 PCT/US2001/013023 US0113023W WO0181375A3 WO 2001081375 A3 WO2001081375 A3 WO 2001081375A3 US 0113023 W US0113023 W US 0113023W WO 0181375 A3 WO0181375 A3 WO 0181375A3
Authority
WO
WIPO (PCT)
Prior art keywords
dna
protein binding
miniature proteins
polypeptide
binding miniature
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2001/013023
Other languages
French (fr)
Other versions
WO2001081375A2 (en
WO2001081375A9 (en
Inventor
Shrader Alanna Schepartz
Jason W K Chin
Reena Zutshi
Stacey Erin Rutledge
Martin Joanne D Kehlbeck
Neal Joseph Zondlo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yale University
Original Assignee
Yale University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yale University filed Critical Yale University
Priority to EP01927291A priority Critical patent/EP1309680B1/en
Priority to AU2001253758A priority patent/AU2001253758A1/en
Priority to DE60118935T priority patent/DE60118935D1/en
Priority to JP2001578462A priority patent/JP2004528802A/en
Priority to CA002407377A priority patent/CA2407377A1/en
Publication of WO2001081375A2 publication Critical patent/WO2001081375A2/en
Anticipated expiration legal-status Critical
Publication of WO2001081375A9 publication Critical patent/WO2001081375A9/en
Publication of WO2001081375A3 publication Critical patent/WO2001081375A3/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/465—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from birds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention provides a protein scaffold, such as an avian pancreatic polypeptide, that can be modified by substitution of two or more amino acid residues that are exposed on the alpha helix domain of the polypeptide when the polypeptide is in a tertiary form.
PCT/US2001/013023 2000-04-24 2001-04-24 Dna & protein binding miniature proteins Ceased WO2001081375A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP01927291A EP1309680B1 (en) 2000-04-24 2001-04-24 Dna & protein binding miniature proteins
AU2001253758A AU2001253758A1 (en) 2000-04-24 2001-04-24 DNA and protein binding miniature proteins
DE60118935T DE60118935D1 (en) 2000-04-24 2001-04-24 DNA & PROTEIN BINDING MINIATURE PROTEINS
JP2001578462A JP2004528802A (en) 2000-04-24 2001-04-24 Small proteins that bind DNA and proteins
CA002407377A CA2407377A1 (en) 2000-04-24 2001-04-24 Dna & protein binding miniature proteins

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US19940800P 2000-04-24 2000-04-24
US60/199,408 2000-04-24
US24056600P 2000-10-16 2000-10-16
US60/240,566 2000-10-16
US26509901P 2001-01-30 2001-01-30
US60/265,099 2001-01-30
US27136801P 2001-02-23 2001-02-23
US60/271,368 2001-02-23

Publications (3)

Publication Number Publication Date
WO2001081375A2 WO2001081375A2 (en) 2001-11-01
WO2001081375A9 WO2001081375A9 (en) 2003-01-16
WO2001081375A3 true WO2001081375A3 (en) 2003-03-13

Family

ID=27498329

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/013023 Ceased WO2001081375A2 (en) 2000-04-24 2001-04-24 Dna & protein binding miniature proteins

Country Status (8)

Country Link
US (2) US7297762B2 (en)
EP (1) EP1309680B1 (en)
JP (1) JP2004528802A (en)
AT (1) ATE323763T1 (en)
AU (1) AU2001253758A1 (en)
CA (1) CA2407377A1 (en)
DE (1) DE60118935D1 (en)
WO (1) WO2001081375A2 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7495070B2 (en) 2000-04-24 2009-02-24 Yale University Protein binding miniature proteins
US6822073B2 (en) * 2001-12-20 2004-11-23 Kimberly-Clark Worldwide, Inc. Modular peptide-based reagent
US20040197893A1 (en) * 2002-10-16 2004-10-07 Carsten Schubert HDM2-inhibitor complexes and uses thereof
CA2544764A1 (en) * 2003-11-04 2005-05-19 Yale University Protein binding miniature proteins
WO2005059131A2 (en) * 2003-12-11 2005-06-30 Yale University Protein binding miniature proteins and uses thereof
JP2008508504A (en) * 2004-07-28 2008-03-21 エフ．ホフマン−ラ ロシュ アーゲー Pancreatic polypeptides as targets / markers for beta cell failure
WO2006093222A1 (en) * 2005-03-02 2006-09-08 Ajinomoto Co., Inc. Inhibitor for insulin polymer formation
AU2011253661B2 (en) * 2005-04-26 2013-06-13 Boehringer Ingelheim Rcv Gmbh & Co Kg Production of recombinant proteins by autoproteolytic cleavage of a fusion protein
EP1874825B1 (en) * 2005-04-26 2010-03-24 Sandoz AG Affinity ligands
WO2009020477A1 (en) * 2007-08-06 2009-02-12 Yale University Modified miniature proteins
PL391627A1 (en) 2010-06-25 2012-01-02 Adamed Spółka Z Ograniczoną Odpowiedzialnością Anticancer fusion protein
EP3782638A1 (en) 2013-03-13 2021-02-24 President and Fellows of Harvard College Stapled and stitched polypeptides and uses thereof
US10227384B2 (en) 2013-04-12 2019-03-12 Yale University Modified proteins and methods of use thereof
CN114805527A (en) * 2014-05-21 2022-07-29 哈佛大学的校长及成员们 RAS inhibitory peptides and their uses
CN109096375A (en) * 2018-08-31 2018-12-28 河南工业大学 A kind of solid phase synthesis process of the polypeptide domain of combination DNA

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5223409A (en) * 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
US5780225A (en) * 1990-01-12 1998-07-14 Stratagene Method for generating libaries of antibody genes comprising amplification of diverse antibody DNAs and methods for using these libraries for the production of diverse antigen combining molecules
US5427908A (en) * 1990-05-01 1995-06-27 Affymax Technologies N.V. Recombinant library screening methods
GB9015198D0 (en) * 1990-07-10 1990-08-29 Brien Caroline J O Binding substance
US5698426A (en) * 1990-09-28 1997-12-16 Ixsys, Incorporated Surface expression libraries of heteromeric receptors
DE69129154T2 (en) * 1990-12-03 1998-08-20 Genentech, Inc., South San Francisco, Calif. METHOD FOR ENRICHING PROTEIN VARIANTS WITH CHANGED BINDING PROPERTIES
PT100379B (en) * 1991-04-10 1999-01-29 Scripps Research Inst LIBRARIES OF HETERODYMERIC RECEPTORS USING FAGOMIDEOS
US5733743A (en) * 1992-03-24 1998-03-31 Cambridge Antibody Technology Limited Methods for producing members of specific binding pairs
US5624949A (en) * 1993-12-07 1997-04-29 Eli Lilly And Company Protein kinase C inhibitors
KR0148439B1 (en) * 1994-03-11 1998-11-02 마사까쓰 마쓰모또 1, 2 dioxetane derivatives
US5516637A (en) * 1994-06-10 1996-05-14 Dade International Inc. Method involving display of protein binding pairs on the surface of bacterial pili and bacteriophage
US5656725A (en) * 1995-05-12 1997-08-12 Apoptosis Technology, Inc. Peptides and compositions which modulate apoptosis
JP2978435B2 (en) * 1996-01-24 1999-11-15 チッソ株式会社 Method for producing acryloxypropyl silane

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
CHIN JASON W ET AL: "Methodology for optimizing functional miniature proteins based on avian pancreatic polypeptide using phage display.", BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, vol. 11, no. 12, 18 June 2001 (2001-06-18), pages 1501 - 1505, XP002196761, ISSN: 0960-894X *
CHIN, J.W. AND SCHEPARTZ, A.: "Concerted evolution of structure and function in a", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 28 March 2001 (2001-03-28), pages 2929 - 2930, XP002196758 *
MCCAFFERTY J ET AL: "PHAGE ANTIBODIES FILAMENTOUS PHAGE DISPLAYING ANTIBODY VARIABLE DOMAINS", NATURE (LONDON), vol. 348, no. 6301, 1990, pages 552 - 554, XP002196759, ISSN: 0028-0836 *
NYGREN PER-AKE ET AL: "Scaffolds for engineering novel binding sites in proteins.", CURRENT OPINION IN STRUCTURAL BIOLOGY, vol. 7, no. 4, 1997, pages 463 - 469, XP002196760, ISSN: 0959-440X *
WU HERREN ET AL: "Building zinc fingers by selection: Toward a therapeutic application.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 92, no. 2, 1995, 1995, pages 344 - 348, XP002196757, ISSN: 0027-8424 *
ZONDLO, N.J. AND SCHEPARTZ, A.: "Highly Specific DNA Recognition by a Designed Miniature Protein", J. AM CHEM. SOC., vol. 121, 1999, pages 6938 - 6939, XP002196756 *

Also Published As

Publication number Publication date
EP1309680A2 (en) 2003-05-14
US20080108789A1 (en) 2008-05-08
WO2001081375A2 (en) 2001-11-01
EP1309680B1 (en) 2006-04-19
US20030166240A1 (en) 2003-09-04
WO2001081375A9 (en) 2003-01-16
DE60118935D1 (en) 2006-05-24
AU2001253758A1 (en) 2001-11-07
US7297762B2 (en) 2007-11-20
ATE323763T1 (en) 2006-05-15
JP2004528802A (en) 2004-09-24
CA2407377A1 (en) 2001-11-01

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