WO2001081596A3 - Methods for enhancing the expression of a protein of interest by recombinant host cells - Google Patents

Methods for enhancing the expression of a protein of interest by recombinant host cells Download PDF

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Publication number
WO2001081596A3
WO2001081596A3 PCT/US2001/013326 US0113326W WO0181596A3 WO 2001081596 A3 WO2001081596 A3 WO 2001081596A3 US 0113326 W US0113326 W US 0113326W WO 0181596 A3 WO0181596 A3 WO 0181596A3
Authority
WO
WIPO (PCT)
Prior art keywords
enhancing
expression
methods
interest
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2001/013326
Other languages
French (fr)
Other versions
WO2001081596A2 (en
Inventor
Si Lok
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zymogenetics Inc
Original Assignee
Zymogenetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zymogenetics Inc filed Critical Zymogenetics Inc
Priority to EP01928859A priority Critical patent/EP1276881B1/en
Priority to JP2001578667A priority patent/JP2003530886A/en
Priority to AU2001255671A priority patent/AU2001255671A1/en
Priority to DE60117375T priority patent/DE60117375T2/en
Priority to CA002407710A priority patent/CA2407710A1/en
Publication of WO2001081596A2 publication Critical patent/WO2001081596A2/en
Publication of WO2001081596A3 publication Critical patent/WO2001081596A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The increased use of nucleotide sequence data mining techniques has amplified the demand for efficient methods of producing recombinant proteins in eukaryotic cells. A strategy is provided for enhancing the synthesis of recombinant amino acid sequences by polymerizing expression cassettes in vitro before producing recombinant hosts.
PCT/US2001/013326 2000-04-26 2001-04-25 Methods for enhancing the expression of a protein of interest by recombinant host cells Ceased WO2001081596A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP01928859A EP1276881B1 (en) 2000-04-26 2001-04-25 Methods for enhancing the expression of a protein of interest by recombinant host cells
JP2001578667A JP2003530886A (en) 2000-04-26 2001-04-25 Method for improving expression of a target protein by recombinant host cells
AU2001255671A AU2001255671A1 (en) 2000-04-26 2001-04-25 Methods for enhancing the expression of a protein of interest by recombinant host cells
DE60117375T DE60117375T2 (en) 2000-04-26 2001-04-25 METHOD FOR INCREASING EXPRESSION OF AN INTERESTING PROTEIN BY RECOMBINANT WIRT CELLS
CA002407710A CA2407710A1 (en) 2000-04-26 2001-04-25 Methods for enhancing the expression of a protein of interest by recombinant host cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US55883000A 2000-04-26 2000-04-26
US09/558,830 2000-04-26

Publications (2)

Publication Number Publication Date
WO2001081596A2 WO2001081596A2 (en) 2001-11-01
WO2001081596A3 true WO2001081596A3 (en) 2002-04-18

Family

ID=24231180

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/013326 Ceased WO2001081596A2 (en) 2000-04-26 2001-04-25 Methods for enhancing the expression of a protein of interest by recombinant host cells

Country Status (7)

Country Link
EP (1) EP1276881B1 (en)
JP (1) JP2003530886A (en)
AT (1) ATE318317T1 (en)
AU (1) AU2001255671A1 (en)
CA (1) CA2407710A1 (en)
DE (1) DE60117375T2 (en)
WO (1) WO2001081596A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3176263A1 (en) 2007-08-10 2017-06-07 Toto Ltd. Method of producing recombinant mammalian cells
JP5800176B2 (en) * 2011-02-01 2015-10-28 国立大学法人広島大学 Method for controlling and amplifying the target gene amplification structure in mammalian cells
JP6770224B2 (en) 2015-01-16 2020-10-14 国立研究開発法人産業技術総合研究所 Gene expression system using stealth RNA and gene transfer / expression vector containing the RNA
JP6544565B2 (en) * 2015-04-28 2019-07-17 国立大学法人広島大学 Method and kit for enhancing expression of target gene in mammalian cells and use thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4403036A (en) * 1980-12-02 1983-09-06 University Of Iowa Research Foundation Genetic reagents for generating plasmids containing multiple copies of DNA segments
US5238820A (en) * 1985-11-05 1993-08-24 Genetics Institute, Inc. Multiply-amplifiable vectors for high level expression of exogenuos DNA

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4403036A (en) * 1980-12-02 1983-09-06 University Of Iowa Research Foundation Genetic reagents for generating plasmids containing multiple copies of DNA segments
US5238820A (en) * 1985-11-05 1993-08-24 Genetics Institute, Inc. Multiply-amplifiable vectors for high level expression of exogenuos DNA

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
ASSELBERGS F A M ET AL: "GENE CASSETTES FOR DIRECTIONAL INSERTION AT THE S L CLEAVAGE SITE IN THE SV40 REPLICATION ORIGINOF MAMMALIAN EXPRESSION VECTORS", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, SAN DIEGO, CA, US, vol. 243, no. 2, 1996, pages 285 - 288, XP000872270, ISSN: 0003-2697 *
HITOSHI IKEDA ET AL: "MULCOS: A VECTOR FOR AMPLIFICATION AND SIMULTANEOUS EXPRESSION OF TWO FOREIGN GENES IN MAMMALIAN CELLS", GENE, ELSEVIER BIOMEDICAL PRESS. AMSTERDAM, NL, vol. 71, no. 1, 15 November 1988 (1988-11-15), pages 19 - 27, XP000000302, ISSN: 0378-1119 *
KIM S C ET AL: "AMPLIFICATION OF CLONED DNA AS TANDEM MULTIMERS USING BSPMI-GENERATED ASYMMETRIC COHESIVE ENDS", GENE (AMSTERDAM), vol. 71, no. 1, 1988, pages 1 - 8, XP002188576, ISSN: 0378-1119 *
MONACO LUCIA ET AL: "An in vitro amplification approach for the expression of recombinant proteins in mammalian cells.", BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, vol. 20, no. 2, 1994, pages 157 - 171, XP001053058, ISSN: 0885-4513 *
SZYBALSKI W ET AL: "CLASS-IIS RESTRICTION ENZYMES - A REVIEW", GENE, ELSEVIER BIOMEDICAL PRESS. AMSTERDAM, NL, vol. 100, 1991, pages 13 - 26, XP002074512, ISSN: 0378-1119 *
TAKESHITA S ET AL: "TANDEM GENE AMPLIFICATION IN-VITRO FOR RAPID AND EFFICIENT EXPRESSION IN ANIMAL CELLS", GENE (AMSTERDAM), vol. 71, no. 1, 1988, pages 9 - 18, XP001042341, ISSN: 0378-1119 *

Also Published As

Publication number Publication date
EP1276881A2 (en) 2003-01-22
ATE318317T1 (en) 2006-03-15
JP2003530886A (en) 2003-10-21
WO2001081596A2 (en) 2001-11-01
EP1276881B1 (en) 2006-02-22
DE60117375T2 (en) 2006-10-19
CA2407710A1 (en) 2001-11-01
DE60117375D1 (en) 2006-04-27
AU2001255671A1 (en) 2001-11-07

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